MicroRNA-139-5p inhibits cell proliferation and invasion by targeting insulin-like growth factor 1 receptor in human non-small cell lung cancer

Introduction: Increasing evidence suggested that microRNAs (miRNAs) play a critical role in tumorigenesis. Decreased expression of miRNA-139-5p has been observed in various types of cancers. However, the biological function of miRNA-139-5p in non-small cell lung cancer (NSCLC) is still largely unknown. Methods: Quantitative real-time PCR (qRT-PCR) was used to explore the expression level of miRNA-139-5p in NSCLC tissues and cell lines. Then, we investigated the role of miRNA-139-5p to determine its potential roles on lung cancer cell proliferation, migration and invasion in vitro. A luciferase reporter assay was performed to confirm the target gene of miRNA-139-5p and the results were validated in renal cancer cells. Results: miRNA-139-5p was significantly decreased in NSCLC tissues and cell lines. Over-expression of miRNA-139-5p could inhibit lung cancer cell proliferation, migration, and invasion in vitro. Furthermore, we identified insulin-like growth factor 1 receptor (IGF1R) as a target of miR-139-5p and miR-139-5p function as a tumor suppressor via targeting IGF1R in NSCLC. Conclusions: Our results indicated that miR-139-5p acts as a tumor suppressor in NSCLC partially via down-regulating IGF1R expression.     

Efficacy of artemether and artesunate in mice infected with praziquantel non-susceptible isolate of Schistosoma japonicum

Praziquantel is currently the only drug of choice for the treatment of human Schistosoma japonicum infections, and praziquantel-based chemotherapy has been proved to be generally effective to control the morbidity and reduce the prevalence and intensity of S. japonicum infections. However, the potential emergence of praziquantel resistance in S. japonicum seriously threatens the elimination of this neglected tropical disease in China. The purpose of this study was designed, in mouse animals, to evaluate the in vivo efficacy of artemether and artesunate against praziquantel non-susceptible S. japonicum. Mice infected with a praziquantel non-susceptible isolate and a praziquantel-susceptible isolate of S. japonicum were treated with artemether and artesunate at a single oral dose of 300 mg/kg given once on each of days 7–8 and 35–36 post-infection to assess the efficacy against juvenile and adult worms. Administration with artemether and artesunate at a single oral dose of 300 mg/kg on each of days 7–8 post-infection resulted in total worm burden reductions of 72.8 and 73.5 % in mice infected with praziquantel-susceptible S. japonicum, and 77.9 and 74.1 % in mice infected with the non-susceptible isolate (both P values >0.05), while the same treatments given on days 35–36 post-infection reduced total worm burdens by 71.4 and 69.6 % in mice infected with the susceptible isolate, and 75.3 and 69.6 % in mice infected with the non-susceptible parasite (both P values >0.05). It is concluded that there is no evidence for reduced susceptibility of artemether and artesunate in praziquantel non-susceptible S. japonicum.     

神经外科术后静脉栓塞症发病率及早期诊断

目的对神经外科术后患者静脉栓塞症（深静脉血栓和肺栓塞）发病率及早期诊断进行前瞻性研究。方法37例神经外科术后患者均按照静脉血栓预防指南进行了弹力袜和（或）四肢间隙气动压迫的预防性治疗。在术后平均12d进行血管彩色超声检查,并通过增强CT扫描明确诊断。结果本组研究中下肢深静脉血栓发生率为13．5％,在发生深静脉血栓患者中肺栓塞发生率为60％,所有静脉血栓患者在临床上均没有症状。结论在神经外科术后患者中静脉血栓症的发病率很高,在采用了预防措施后部分患者依然不能避免,早期预防和早期发现对于减少神经外科术后静脉栓塞症非常重要。     

成肌细胞-PLGA支架复合物修复比格犬半月板损伤的实验研究

目的 探讨成肌细胞种植于聚乳酸聚乙酸共聚物（PLGA）支架上,软骨定向诱导后植入比格犬体内修复半月板缺损的可能性.方法 将来源于比格犬的成肌细胞培养传代至第4代,收集后以5.0×106细胞/cm3材料体积密度将细胞接种于PLGA支架中,含50 ng/ml软骨源性形态发生蛋白-2（CDMP-2）和20 ng/ml转化生长因子β1（TGF-β1）软骨诱导培养基培养14 d.然后将细胞-支架复合体植入24只比格犬半月板缺损模型中,术后4、8、12周取材,采用大体观察、组织学、生物化学和生物力学作为评估指标.结果 术后4、8、12周各项检测结果显示PLGA支架材料逐渐降解吸收,而成肌细胞可逐渐合成分泌新生胶原,并最终形成半月板样纤维组织,而对照各组缺损区则仅见少许纤维组织样的修复组织.结论 利用成肌细胞经体外扩增及细胞因子刺激后,以PLGA支架为载体,按一定的细胞密度复合培养后,植入关节腔内修复半月板局部缺损的方法,是一种较为可行的修复半月板损伤的方法.     

临床药师参与1例肝衰竭患者糖皮质激素治疗的分析与药学监护

目的 探讨临床药师参与患者药物治疗方案的制订和药学监护的方法。方法 结合1例乙型肝炎相关慢加急性肝功能衰竭患者的糖皮质激素治疗方案,分析糖皮质激素治疗的机制、应用时机、剂量疗程,并对相关不良反应进行药学监护。结果 临床药师的意见被采纳,患者经治疗后病情趋于好转。结论 临床药师参与患者治疗及药学监护,协助医生进行给药方案的调整,有助于药物治疗的安全性和有效性。     

聚丙烯酰胺水凝胶注射隆乳后的并发症及治疗

目的探讨聚丙烯酰胺水凝胶（polyacrylamide hydrogel,PAHG）注射隆乳后的并发症及治疗方法。方法对l74例术前行乳房超声或磁共振成像（MRI）检查以确定PAHG分布范围。采用乳晕切口行组织分离,生理盐水灌洗,清除PAHG,切除异常组织;其中对继发乳房畸形者选择性地以胸大肌后间隙假体置人进行修复87例（50％,48例I期,39例Ⅱ期）。结果术后随访3～6个月,绝大部分症状得到缓解。77例（44．3％）对乳房外形、手感满意;10例（5．7％）对手感不太满意;79例（45．4％）出现乳房下垂或扁平胸;8例（4．6oA）出现较重的乳房畸形。结论PAHG注射隆乳术后出现并发症者,应尽早清除,治疗方法得当可取得较好疗效,但完全满意者较少。穿刺盲视抽吸的方法不可取,以开放式手术为佳,有强烈要求及条件允许者可行乳房假体置人重建术。     

对国家基本药物集中采购价格政策的逆向思考与建议

目的解析基本药物集中采购过程中存在主要问题及其原因,并针对价格问题提出有效的解决方案,以确保基本药物的质量与正常供应,有效降低患者负担,保障患者用药安全。方法对6家三级医院的基本药物集中采购实施情况进行调查,对存在主要突出问题进行归纳分析,找出其内在原因并提出相应解决方案。结果基本药物集中采购工作进展较顺利,但在实施过程中也存在因实行最低价中标原则导致部份产品价格过低而产生药品质量与药品供应难以保障等诸多问题。结论基本药物集中采购的价格政策不能简单地以最低价中标,而应当科学地、全面地分析制定策略。建议实行平均价格区间内低价中标,适当放宽差率控制,既能降低药品价格,也能避免恶性竞争,药品质量和生产供应也得到保障,同时医疗机构也愿意更多地使用,从而真正减轻患者负担,缓解看病贵的难题。     

供应驱动角度的双向转诊协同体系构建

双向转诊是有效配置区域上下级医疗机构间的医疗服务资源,构建合理分层就诊体系的重要举措.从供应驱动角度分析了双向转诊协同概念,指出了存在问题,提出了构建方法：完善供应链体系;协调供需平衡;信息实时共享;政策引导支持;与需求驱动互补等.     

HP5500心脏彩超故障维修一例

本文分析了HP5500心脏彩超的工作原理,介绍了1例显示器黑屏故障的原因及维修方法。     

强脉冲光治疗面部毛细血管扩张症108例疗效分析

目的:探讨强脉冲光治疗后,面部不同程度毛细血管扩张症患者的治疗效果和最终满意度。方法:采用Lumenis One激光与强脉冲光操作系统对不同程度毛细血管扩张症患者进行治疗,观察局部毛细血管和总体皮肤改善情况,并在疗程结束后1个月进行满意度回访。结果:经5次治疗(1个疗程)后,轻、中度毛细血管扩张症有效率达100%,重度有效率88.9%,总有效率98.1%,患者满意度为99.1%。结论:强脉冲光治疗面部毛细血管扩张症疗效明显,同时改善皮肤衰老状态,患者满意度高。