Icariin Improves Cognitive Impairment after Traumatic Brain Injury by Enhancing Hippocampal Acetylation

Objective

To examine the effect of icariin (ICA) on the cognitive impairment induced by traumatic brain injury (TBI) in mice and the underlying mechanisms related to changes in hippocampal acetylation level.

Methods

The modifified free-fall method was used to establish the TBI mouse model. Mice with post-TBI cognitive impairment were randomly divided into 3 groups using the randomised block method (n=7): TBI (vehicle-treated), low-dose (75 mg/kg) and high-dose (150 mg/kg) of ICA groups. An additional sham-operated group (vehicle-treated) was employed. The vehicle or ICA was administrated by gavage for 28 consecutive days. The Morris water maze (MWM) test was conducted. Acetylcholine (ACh) content, mRNA and protein levels of choline acetyltransferase (ChAT), and protein levels of acetylated H3 (Ac-H3) and Ac-H4 were detected in the hippocampus.

Results

Compared with the sham-operated group, the MWM performance, hippocampal ACh content, mRNA and protein levels of ChAT, and protein levels of Ac-H3 and Ac-H4 were signifificantly decreased in the TBI group (P<0.05). High-dose of ICA signifificantly ameliorated the TBI-induced weak MWM performance, increased hippocampal ACh content, and mRNA and protein levels of ChAT, as well as Ac-H3 protein level compared with the TBI group (P<0.05).

Conclusion

ICA improved post-TBI cognitive impairment in mice by enhancing hippocampal acetylation, which improved hippocampal cholinergic function and ultimately improved cognition.

     

彩色多普勒超声对缺血性眼病的临床诊断意义

目的:探讨彩色多普勒超声对缺血性眼病的临床诊断意义。方法:采用IU22型超声诊断仪对40例已确诊的单眼缺血性眼病患者40眼,对照组采用患者的另一相对健康眼40眼及正常人40例40眼,进行颈内动脉、眼动脉、视网膜中央动脉检查。结果:缺血性眼病患者的患眼的眼动脉、视网膜中央动脉的收缩期血流峰值速度(peak systolic velocities,PSV)、舒张末期血流速度(end diustolic velocities,EDV)、血管内径(blood vessel diameter,BVD)明显低于患者的健康眼及正常人的眼(P<0.05);阻力指数(resistance index,RI)则明显增加(P<0.05);三组比较,颈内动脉测量结果,患者患眼及健康眼较正常人组仅阻力指数明显增加(P<0.05),其它检测结果无统计意义。结论:缺血性眼病患者患眼眼动脉,视网膜中央动脉PSV,EDV,BVD均下降,RI增加。彩色多普勒对缺血性眼病是一种比较敏感的检测手段。     

基于“互联网+”的医共体共享中药房服务患者满意度调查与分析△

目的:调查和分析基于"互联网+"的医共体共享中药房患者满意度及其影响因素,为评价和提高共享中药房服务质量提供参考。方法:采用问卷调查的形式收集医共体内196名就诊患者的满意度信息,并对其进行统计分析。结果:196名患者就诊总体满意度为79.93%,其中116名患者接受过共享中药房服务,其就诊总体满意度为81.90%,共享中药房服务总体满意度为88.79%,通过多重线性回归分析发现代煎服务中药口感和配送及时性对共享中药房服务总体满意度存在显著性影响(P <0.05)。结论:医共体共享中药房服务患者满意度总体状况较好,共享中药房在改善基层医疗卫生机构中药服务能力的同时,还要重视质量监管体系的完善,提升服务质量。     

Clinical characteristics and surgical outcomes of ependymomas in the upper cervical spinal cord: a single-center experience of 155 consecutive patients

Neurosurgical Review - Ependymomas occurring in the upper cervical spinal cord (above the level of the C4 segment) are rare entities with great therapeutic challenges. This study was aimed to...     

清热疏肝调周法治疗女性面部痤疮62例

根据中医理论及临床经验,作者认为湿热内蕴,肝气郁结,冲任失调为女性痤疮多发的重要病机,治疗以清热疏肝调周为法,并有针对性地对患者进行心理疏导及心理干预,使其压力得以缓解,心情舒畅,收效良好。     

黄煌经方内科医案(七)——便秘治验3则

便秘为中医临床常见症,可见于多种疾病。其病虽小,但患者关注度较高。黄煌老师擅长识人辨体,临证或从体质辨证切入,或从心神调节入手,选用相应经方治疗,取效显著。现试析黄师治疗本病验案3则,供同道参考。     

恩度联合奈达铂胸腔内灌注治疗恶性胸腔积液疗效观察

目的探讨恩度联合奈达铂胸腔内灌注治疗恶性胸腔积液的有效性和安全性。方法将60例恶性胸腔积液患者随机分为试验组和对照组,2组患者均先采用胸腔穿刺置管闭式引流胸腔积液,试验组30例采用恩度（45mg）联合奈达铂（40mg）进行胸腔内灌注,而对照组30例仅采用奈达铂（40mg）进行胸腔内灌注,观察2组的疗效和毒副反应。结果试验组有效率为76．67％,优于对照组的36．67％,差异有统计学意义（P〈0．05）;2组主要毒副反应发生率比较差异无统计学意义（P均〉0．05）;试验组生活质量改善率为73．33％,高于对照组的43．33％,差异有统计学意义（P〈0．05）。结论恩度联合奈达铂胸腔内灌注治疗恶性胸腔积液安全有效。     

脓毒血症患者免疫调理治疗研究

目的探讨胸腺肽α1联合血必净对脓毒血症患者免疫功能的影响。方法72例脓毒症患者随即分成治疗组和对照组,对照组参照拯救脓毒症运动中所建议经典治疗方法,治疗组经典治疗基础上加胸腺肽α1联合血必净进行免疫调理治疗,比较两组患者治疗前后APACHEⅡ评分,血浆IL-6水平,T淋巴细胞亚群构成变化,及外周血CD14+单核细胞人类白细胞抗原(HLA-DR)水平变化。结果治疗组患者经免疫调理治疗后APACHEⅡ评分显著降低,其CD14+单核细胞HLA-DR和T细胞亚群CD4+、Th/Ts显著提高,IL-6表达水平明显下降,与对照组比较差异具有显著性(P<0.05)。结论胸腺肽α1联合血必净治疗脓毒症患者,有助于增强机体的免疫防御作用,并抑制过度炎症反应。     

白血病患者的p53基因缺失及临床意义

目的探讨白血病患者的p53基因缺失情况及其临床意义。方法使用I-FISH回顾性分析37例复杂核型异常及25例非复杂核型异常的白血病患者的p53基因异常的情况。结果 37例复杂核型异常的白血病患者p53基因的缺失频率为46.01%（17/37）,25例非复杂核型异常的缺失频率为4.00%（1/25）,两者之间差异有统计学意义（P〈0.01）。其中13例伴复杂核型的急性白血病患者完全缓解率（CR）15.40%,中位生存时间（MST）105d。结论 I-FISH可以快速、准确、灵敏的检测出p53基因的缺失,伴复杂核型的白血病患者p53基因缺失频率较高,CR率低,MST较短。