中医治疗传染性非典型肺炎的优势

通过亲临非典一线临床工作，认识到中医治疗非典的优势是：中医药早期介入后，退热快，症状明显减轻；可避免激素应用或减少用量，纠正激素的副作用；采用活血化瘀、软坚散结等治疗方法，可及早阻止或减轻肺纤维化的形成；祛邪扶正辨证而施，恢复期以益气养阴法为主。     

The selective tyrosine kinase-inhibitor nilotinib alleviates experimentally induced cisplatin nephrotoxicity and heptotoxicity

This work tested the action of nilotinib, selective inhibitor of tyrosine kinase on cisplatin (CP)-induced damage of kidney and liver in rats. Rats were assigned to 4 groups, control, nilotinib, CP, and CP plus nilotinib. Assessment of kidney and liver function, lipid peroxidation and antioxidant markers, anti-apoptotic protein Bcl2, nuclear factor- kappa B (NF-κB) immunoreactivity, and caspase 3 activity were done. CP-induced damage evidenced by histopathological changes, deterioration of renal and liver function, imbalance in oxidants/antioxidants markers, decreased Bcl2, increased caspase 3 activity, and NF-κB nuclear expression in both organs. Nilotinib treatment with CP restored kidney and liver oxidants/antioxidant levels also increased Bcl2 and decreased NF-κB immunoreactivity were evident with nilotinib treatment. In conclusions these results demonstrated a protective effect of nilotinib in experimentally induced CP kidney and liver damage that could be mediated through combating oxidative stress, reducing inflammation and anti-apoptosis in the two organs.     

针刺和聚焦冲击波疗法对肩袖肌腱病的止痛作用:一项回顾性临床研究

ObjectiveThe purpose of the study is to evaluate whether the clinical effects of focused extracorporeal shockwave therapy (f-ESWT) and acupuncture anticipate the clinical response in patients with rotator cuff tendinopathy in the short term.DesignAn observational retrospective clinical study.Setting/LocationPhysical Medicine and Rehabilitation Unit, Sant'Andrea University Hospital in Rome, Italy.Subjects/InterventionsWe analyzed retrospectively thirty patients (22 females and 8 males) with rotator cuff tendinopathy.Fifteen patients underwent f-ESWT and acupuncture combined therapy (Group A) and fifteen patients underwent only f-ESWT therapy (Group B).Outcome measuresThe outcome measures were the Visual Analogic Scale (VAS), Assessment Shoulder and Elbow Scale (ASES) and Roles and Maudsley Score (RMS). The follow-ups were T0 (pre-treatment), T1 (3 weeks after the start of the treatment), and T2 (at 8 weeks). In order to compare groups, the ANOVA and Friedman tests were adopted.Group A patients had a more rapid and statistically significant improvement trend in VAS (P <0.001), ASES scale (P <0.001), and a higher level of satisfaction to treatment assessed by RMS (P<0.001) than group B in the short term.ConclusionsThe study showed that combined treatment decreases the recovery time of the shoulder involved in terms of pain and motor function in the short term. However, these results shall be confirmed by controlled randomized studies.     

Knockdown of Nestin inhibits proliferation and migration of colorectal cancer cells

Nestin, a member of type VI intermediate filament protein family, is widely expressed in mammalian nervous tissue and stem/precursor cells of non-neuronal normal tissues. Nestin has also been investigated to determine possible tumor-promoting functions. However, whether Nestin is involved in colorectal cancer (CRC) cells remains unclear. In this report, Nestin expression was upregulated in stromal cells of human CRC tissues. Endogenous Nestin expression in CRC cell lines SW480 and HCT116 was knocked down by a lentivirus. MTT and colony formation assays revealed that Nestin deletion significantly inhibits the proliferation of CRC cell lines; flow cytometer analysis showed that Nestin deletion causes cell cycle arrest at S phase. Transwell chamber and wound healing scratch assays also revealed that Nestin deletion suppresses cell migration. Our findings indicated that Nestin plays an essential role in CRC progression; thus, Nestin can be applied as a therapeutic target of CRC.     

沈丕安辨治狼疮性肾炎经验

沈丕安教授系上海市名中医,全国著名的风湿病专家,曾任中华中医药学会风湿病分会主任委员.从医近50载,擅长运用中医药治疗自身免疫性疾病,尤其是在运用中药治疗系统性红斑狼疮方面,有着独到的见解和丰富的经验,并结合现代药理研究甄选出具有较强免疫抑制作用的中药治疗狼疮性肾炎,可明显改善蛋白尿,疗效显著,不良反应少.笔者有幸师...     

栀子水提液对小鼠的肝毒性及健脾保肝方的预防作用

目的：探讨栀子对小鼠肝脏的毒性及健脾保肝方预防作用。方泼：①分别以高、中、低剂量的栀子水提液每天灌胃c57bl雄性小鼠,2周后逐批处死,观察肝脏病理学和血清生化指标的变化;②制作小鼠高剂量栀子肝损伤模型,同时以健脾保肝方配伍高剂量栀子的水提液灌胃,并与同期正常和单用健脾保肝方的小鼠对照,观察肝脏病理学和血清生化指标的变化。结果：①高剂量的栀子水提液（相当于临床成人剂量36g）应用6周,对肝细胞的损伤很明显,而中、低剂量的栀子则没有明显影响;高剂量组在第4周时ALT与第6周时AST活性较正常组明显升高（P〈0．05,P〈0．01）。②高剂量栀子水提液造成小鼠明显的肝细胞水样变和ALT、AST、TBiL等生化指标异常（P〈0．05）,而健脾保肝方配伍高剂量栀子的水提液造成的小鼠肝组织病理改变较轻,ALT、AST活性及TBiL含量较高剂量组低,但无统计学差异。结论：高剂量栀子水提液可以导致正常小鼠的肝损伤;健脾保肝方尚不足以对抗高剂量栀子的肝损伤。     

杞枣口服液治疗小儿厌食90例

目的对杞枣口服液治疗小儿厌食脾胃气虚型的临床有效性及安全性进行初步观察。方法观察3家医院儿科门诊符合小儿厌食西医诊断标准及中医脾胃气虚型标准者90例,服用杞枣口服液,疗程1月。结果符合方案集87例,杞枣口服液治疗厌食疗效评价总有效率82.7%;中医证候疗效总有效率95.4%。全分析集90例,杞枣口服液治疗厌食疗效评价有效率80.0%;中医证候疗效有效率92.2%。结论杞枣口服液有健脾益肾之效,对于厌食脾胃气虚型患儿有一定疗效。     

耳甲电针对冷应激大鼠脂蛋白代谢的调节作用

ObjectiveTo explore whether auricular concha eletroacupuncture (ACEA) is effective in regulating lipid-lipoprotein metabolism in rats submitted to cold stress.MethodsThirty-six adult male Sprague–Dawley rats were placed in four groups(9 rats in each group), the rats in three groups of which were submitted to cold stress for fourteen days, the last one of which was a control group. After the cold stress process, in those three groups, the rats of one group were with no treatment (stress only), two were treated with either ACEA or auricular margin eletroacupuncture (AMEA) repeated for fourteen days. On the 14th day, all the rats were sacrificed after all experimental procedure for blood sampling. Blood glucose, triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels were tested by using the collected serum. Plasma lecithin-cholesterol acyltransferase (LCAT) was measured with ELISA kit.ResultsACEA down-regulated the TG level (P < 0.05) and LDL-C level (P < 0.05), and up-regulated HDL-C level (P < 0.05) and LACT level (P < 0.05). AMEA did not regulate the bio-markers.ConclusionACEA played an important role in regulating lipid-lipoprotein metabolism in rats submitted to cold stress.     

Fuzheng Huayu Formula (扶正化瘀方) Prevents Rat Renal Interstitial Fibrosis Induced by HgCl2 via Antioxidative Stress and Down-regulation of Nuclear Factor-Kappa B Activity

Objective: To investigate the mechanism of action of Fuzheng Huayu Formula (扶正化瘀方, FZHY) against renal interstitial fibrosis (RIF) relating to oxidative injury and nuclear factor-kappa B (NF-κB) activity. Methods: Thirty-two Sprague-Dawley rats were randomly divided into 3 groups: normal group, model group and FZHY treatment group. The RIF model was induced by oral administration of HgCl2 at a dose of 8 mg/kg body weight once a day for 9 weeks. Meanwhile, rats in FZHY treatment group orally took FZHY at a dose of 4.0 g/kg rat weight for 9 weeks. The content of hydroxyproline (Hyp) and collagen deposition in kidney were observed. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), the content of glutathione (GSH) and malondialdehyde (MDA) of kidney were tested. The expressions of inhibitor-κappa B (IκB), phospho-IκB (p-IκB), tumor necrosis factor-α (TNF-α), matrix metalloproteinase-2 (MMP-2) and α-smooth muscle actin (α-SMA) were analyzed by Western blot. α-SMA expression was also observed by immunofluorescent staining. MMP-2 activity was measured by gelatin zymography. NF-κB activation was determined by electrophoretic mobility shift assay. Results: Renal interstitial fibrosis was induced by HgCl2, demonstrated by remarkably increased Hyp contents and excessive collagen deposition in kidney (P<0.01). FZHY significantly inhibited renal interstitial collagen deposition and reduced Hyp content of the HgCl2-treated rats (P<0.01). GSH content decreased obviously, and MDA content increased significantly in HgCl2-treated rats compared with that of normal rats (P<0.01). FZHY significantly increased GSH content and decreased MDA content in the model rats (P<0.01). The expression α-SMA was increased in model rats compared with that of normal rats, FZHY significantly decreased its expression (P<0.01). The expressions of p-IκB and TNF-α and MMP-2, MMP-2 activity, and NF-κB activation were increased in model group compared with that in normal group (P<0.01), FZHY significantly decreased NF-κB activation, MMP-2 activity and p-IκB and TNF-α expressions (P<0.01). Conclusions: FZHY could protect kidney from oxidative injury intoxicated by HgCl2, and antagonized oxidative stress-stimulated NF-κB activity through inhibition of IκB phosphorylation in the interstitial fibrotic kidney, these effects importantly contributed to FZHY action mechanism against renal interstitial fibrosis.     

中风解毒方对急性脑缺血大鼠脑梗死面积和神经功能缺损的影响

观察中风解毒方对大鼠局灶性脑缺血再灌注损伤的保护作用。采用大鼠大脑中动脉(MCA)线栓法建立局灶性脑缺血／再灌流模型。设阴性对照组,中风解毒方低、中、高剂量组和补阳还五汤阳性对照组;观察各组大鼠大脑梗死面积及神经功能缺损。结果：中风解毒方能缩小梗死面积、改善神经功能缺损评分,与补阳还五汤作用相似。提示中风解毒方能减轻局灶性脑缺血再灌流损伤,有脑保护作用。