沙利度胺对鼠子宫内膜异位病灶血管生成作用的研究

目的:探讨沙利度胺(thalidomide)对子宫内膜异位症(endometriosis,EMs)病灶生长和血管生成的影响,为从抗血管途径治疗EMs提供依据.方法:将EMs患者在位子宫内膜种植于重度复合性免疫缺陷病(severe combined immunodeficiency disease, SCID)小鼠皮下,建立EMs鼠模型.接种后第3周给予治疗,治疗组(n=10)腹腔注射沙利度胺50mg/kg/d,对照组(n=10)腹腔注射等体积PBS,连用14d;每隔3d测量异位病灶体积一次;病灶组织采用免疫组化法测定病灶微血管密度(microvessel density,MVD)及血管内皮生长因子(VEGF)的表达.结果:SCID小鼠皮下种植内异症模型内膜存活率高且观察方便;治疗组病灶体积增长有缩小趋势,但与对照组相比差异无统计学意义(P＞0.05);治疗组MVD显著低于对照组(P＜0.05);治疗组和对照组VEGF的表达差异无统计学意义(P＞0.05).结论:沙利度胺对EMs异位病灶的血管生成有明显抑制作用;抑制VEGF的表达可能不是沙利度胺抑制EMs血管生成的主要原因.     

自体动静脉内瘘血栓形成的影响因素

目的：了解动静脉内瘘血栓形成的原因，提出防治措施。方法：81例维持性血液透析患有15例内瘘发生血栓形成。分析病因、性别、年龄、血压、血脂对血栓形成的影响。结果：在内瘘使用的早期(三年内)易发生血栓，内瘘血栓发生率为18．52％。老年、男性、糖尿病、血压低、血脂高的患内瘘易发生血栓．结论：定时监测血管通路，适量使用促红索，缓慢提高血红蛋白，恰当地应用抗凝治疗；减慢透析超滤速度，防止透后低血压；采取阶梯式穿刺法，透析后采取点状压迫，防止力量过大，压迫时问过长和对患及家属进行保护内瘘的宣教均是预防内瘘发生血栓的重要措施。     

百蕊草药材与标准汤剂量值传递规律研究及分析

目的 建立百蕊草Thesii Herba标准汤剂的UPLC特征图谱及其指标成分的含量测定方法;研究百蕊草药材与标准汤剂特征图谱的变化,并分析指标成分百蕊草素I在药材-标准汤剂的量值传递规律。方法 采用Waters Acquity UPLC BEH Shield RP18色谱柱（150 mm×2.1 mm,1.7μm）,流动相为乙腈-0.2%甲酸水溶液,梯度洗脱,体积流量0.4 mL/min,检测波长330、270 nm,柱温30℃,建立特征图谱与含量测定方法。以出膏率、含量及转移率、特征图谱共有峰传递数为主要评价指标,分析百蕊草标准汤剂的量值传递规律。结果 19批百蕊草药材、标准汤剂与各自的对照特征图谱相似度分别为0.986～1.000、0.989～1.000。百蕊草标准汤剂特征图谱标定6个共有峰,药材中的5个共有峰全部转移至标准汤剂并被指认,其中4个共有峰采用对照品比对确认,分别为5-O-阿魏酰奎宁酸（峰2）、4-O-阿魏酰奎宁酸（峰3）、百蕊草素I（峰5）、山柰酚-3-O-(2″-O-α-鼠李糖基-6″-O-丙二酰基)-β-葡萄糖苷（峰6）。19批标准汤剂的平均出膏率为27.45%...     

Methylenetetrahydrofolate Reductase Genotypes, Dietary Habits and Susceptibility to Stomach Cancer

OBJECTIVE To study the relation among methylenetetrahydrofolate reductase (MTHFR) C677T genotypes, dietary habits and the risk of stomach cancer (SC).METHODS A case-control study was conducted with 107 cases of SC and 200 population-based controls in Chuzhou district, Huaian, Jiangsu province, China. The epidemiological data were collected, and DNA of peripheral blood leukocytes was obtained from all of the subjects..MTHFR genotypes were detected by PCR-RFLP. RESULTS (1) The prevalence of the MTHFR C/T or T/T genotypes was found to be significantly different between controls (68.5%) and SC cases (79.4%,P=0.0416), the increased risk had an adjusted OR of 1.79 (95?:1.01-3.19). (2) Among subjects who had a low intake of garlic or Chinese onion, MTHFR C/T or T/T genotypes significantly increased the risk of developing SC. Among non-tea drinkers or among subjects who had a frequent intakeof meat, the carriers of the MTHFR C/T or T/T genotypes had a higher risk of SC than individuals with the C/C type MTHFR. CONCLUSION The polymorphism of MTHFR C677T was associated with increased risk of developing SC, and that individuals with differing genotypes may have different susceptibilities to SC, based on their exposure level to environmental factors.     

富硼菊花栽培技术研究

本文通过对菊花施用不同处理的速乐TM和持力硼TM，比较硼肥对菊花中重要的指标绿原酸、槲皮素、木犀草素及总黄酮含量的影响。结果表明：喷施1200倍浓度的速乐硼TM对菊花的中各组分的含量影响与对照（喷清水）相比有显著提高。     

MMED 6000M监护仪开关电源原理及维修

本文介绍了开关电源的组成和工作原理，结合监护仪开关电源的故障实例，分析了故障原因，并最终解决故障。     

俯卧位双切口治疗三踝骨折应用体会

目的 俯卧位下踝关节后外侧入路辅助内侧切口治疗三踝骨折的应用体会。 方法 选取2015年9月—2018年11月安徽省滁州市第一人民医院骨科收治的三踝骨折患者39例，根据Lauge-Hanse分型分为旋后外旋型24例，旋前外展型10例，旋前外旋型3例，旋后内收型1例，垂直压缩型1例，所有患者均采用俯卧位下后外侧入路辅助内侧切口内固定治疗。手术体位均采用俯卧位，后外侧入路先复位固定外踝，外踝复位后，由于后侧关节囊的牵张作用，复位固定后踝相对容易，术中采用“拉钩实验”检查下胫腓联合的稳定性，必要时在外踝处行3层皮质骨螺钉固定下胫腓联合，内固定顺序是外踝、后踝，最后辅助内侧切口复位固定内踝，处理外踝骨折时钢板放置于腓骨后侧或外侧。术中辅以C型臂X线机荧屏监视证实三踝骨折的解剖复位及内固定牢靠。术后随访观察骨折复位、愈合及踝关节功能情况。 结果 39例患者均得到随访，平均随访13(4~26)个月。术后半年全部采用Baird-Jackson踝关节评分系统进行评分，超过95分者10例(25.6％)，介于91~95分者23例(59.0％)，5例评分介于81~90分之间，1例评分低于80分，总优良率为84.6％。 结论 俯卧下后外侧入路辅助内侧切口治疗三踝骨折，操作简单方便，可直视下复位固定后踝骨折块，同时外踝及内踝也能获得良好显露、复位与固定，临床疗效确切，适用于三踝骨折手术。      

毛细管气相色谱法测定化妆品中对羟基苯甲酸酯的含量

目的建立毛细管气相色谱法测定化妆品中的对羟基苯甲酸甲酯和对羟基苯甲酸乙酯。方法采用溶液直接进样法测定,DB-17毛细管柱,FID检测器,分流比10∶1。结果两种防腐剂在10～200μg·mL-1浓度范围内呈良好线性关系,相关系数分别为0.9992、0.9993,两种化合物的加标回收率为95%～98%。结论本法耗时少,分离度高,用标准曲线法测定,方法简单可靠。     

Different effects of soil bacterial communities affected by biocontrol agent YH-07 on tomato Fusarium wilt inhibition

Bio-organic fertilizers based on biocontrol microorganisms have been widely applied to suppress soilborne diseases and improve crop yields. Studies on beneficial biocontrol agents have promoted the development of the bio-organic fertilizers in China. Our previous study demonstrated that a biocontrol agent, Erythrobacter sp. YH-07, can inhibit the growth of the plant pathogen Fusarium oxysporum. In the present study, we investigated the effects of this biocontrol agent on tomato wilt and used the illumina-based sequencing approach to characterize the variations in soil bacterial communities in a potted experiment. The aim of our study was to explore the potential correlation among bacterial communities, Fusarium wilt suppression, and soil properties after application of the biocontrol agent YH-07. The results showed that application of Erythrobacter sp. YH-07 effectively controlled outbreaks of tomato Fusarium wilt. The illumina MiSeq sequencing showed that Proteobacteria was the predominant phylum in the soil samples. Bacterial community composition and structure varied under different soil treatments, e.g., the relative abundance of Erythrobacter and Salinimicrobium was significantly increased in the YH treatment, and Acidobacteria were decreased in the YH treatment compared with the CK treatment. Additionally, the correlation results showed that the soil organic matter and available phosphorus and potassium were higher after YH-07 application, and they were positively correlated with bacterial community. The redundancy analysis showed Erythrobacter and Acidobacteria were the dominant genera after YH and CK treatments, respectively, and correlations with tomato Fusarium wilt incidence were negative and positive, respectively.

The model plant tomato was used to verify the effect and mechanism of a novel discovered strain YH-07 which is a potential agent for biological control of soil borne disease named tomato Fusarium wilt.     

一测多评法同时测定乳癖消片中5种指标性成分的研究

目的:建立一测多评(QAMS)法来测定乳癖消片中5种指标性成分的含量。方法:采用HPLC法,以岛津Inertsil ODS-3(250 mm×4.6 mm,5μm)为色谱柱;流动相为乙腈-水溶液,梯度洗脱;流速:1.0 ml·min^-1;检测波长为可变波长(0 min,230 nm;18 min,203 nm;26.5 min,275 nm;35 min,203 nm);柱温:30℃;进样量:10μl。以连翘苷为参照物建立5种指标性成分之间的相对校正因子,分别采用外标法和一测多评法测定乳癖消片中的5种指标性成分的含量,并对两种方法所得结果进行比较。结果:在一定的线性范围内,连翘苷与芍药苷、人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的相对校正因子为0.411,2.277,3.744,2.776,且在不同试验条件下重现性良好。一测多评法的计算值与外标法测定值间差异无统计学意义(P>0.05)。结论:所建立的QAMS法可用于测定乳癖消片中5种指标性成分的含量。