Lysophosphatidic acid induces human natural killer cell chemotaxis and intracellular calcium mobilization

We have investigated the effects of the bioactive lipid lysophosphatidic acid (LPA) on several functions of activated human natural killer (NK) cells. Flow cytometric and immunoblot analyses show that these cells express LPA(1, )LPA(2) and LPA(3). LPA but not its precursor phosphatidic acid (PA) induces the chemotaxis of NK cells, an activity that is inhibited by prior treatment of the cells with pertussis toxin (PTX). In addition, LPA induces the mobilization of intracellular calcium, an effect that is markedly inhibited by PTX, but is not inhibited by the addition of EGTA. PA also induces calcium flux in NK cells, but with much lower efficacy than LPA. Cross-desensitization experiments demonstrate that LPA and PA utilize different receptors. Moreover, LPA or PA but not sphingosine 1-phosphate, enhances IFN-gamma secretion by activated NK cells. Our results may shed some light on the findings that activated NK cells are found at the sites of tumor growth.     

h1- and h2-calponins are not essential for norepinephrine- or sodium fluoride-induced contraction of rat aortic smooth muscle

To investigate the controversial issue concerning the role of calponin in smooth muscle contraction, this study examined the relationship between smooth muscle calponin and the contraction of aortic rings from different strains of rats: Sprague-Dawley (SD), Wistar, and Wistar Kyoto (WKY). Western blot analysis demonstrated that h1- and h2-calponins are present in aortic smooth muscle from adult SD rats but not Wistar or WKY rats. Nevertheless, h1-calponin is detectable in stomach from Wistar rats, although at a much lower level compared with that in the SD rat stomach. This suggests that a repressed expression of the gene, instead of a simple null mutation, may have caused its absence from the aortic smooth muscle. Despite the presence or absence of calponin, the aortic smooth muscles from the different strains of rats all develop contractions in response to the physiological agonist norepinephrine (NE) and following activation with the plasma membrane receptor-independent NaF induction. The data indicate that h1- and h2-calponins are not essential for NE- and NaF-induced contractions in aortic smooth muscle. The calponin-positive adult SD rat aorta was found to be more sensitive in contractile response to NE and NaF inductions compared with the calponin-negative rat aortae. This may imply a potential modulator function of calponin in the contraction of smooth muscle, whereas other contractile protein isoform differences between these rat strains may also play a role.This revised version was published online in September 2005 with corrections to the Cover Date.     

金针菇子实体多糖提取物对人肝癌SMMC—7721细胞的抑增殖作用

金针菇子实体经热水提取，乙醇沉淀，胰蛋白酶水解，Ｓｅｖａｇ法去除蛋白质，乙醇分级沉淀等处理得金针菇子实体多糖。研究了该多糖对人肝部ＳＭＭＣ－７７２１细胞生长曲线，有丝分裂指数及线粒体活性的影响。结果表明该多糖对体外培养的人肝癌ＳＭＭＣ－７７２１细胞具一定抑制作用。     

猫运动皮层内篮状细胞的立体重构,递质性质及突触分布

用细胞内HRP染色的方法显示该神经元位于猫运动皮层十字沟后,在第Ⅱ/Ⅲ层之间(软膜以下438μm处),胞体截面积为15.6×28.1μm~2,呈多极形,树突呈串珠状,向四周扩展,无侧棘,轴突由胞体下方伸出,返折向上,包绕胞体周围,以软膜平行的吻尾方向行走为主,有一分枝向白质方向延伸.从立体重构图的不同角度观察,其形态不对称,如沿X轴方向旋转60°后,其形态呈扁平形,用胶体金免疫电镜观察,该神经元的递质性质为GABA能的.胞体及树突上分布有非对称型和对称型的突触,对称型的突触中有的是GABA能的,有的是非GABA能的.其轴突有髓鞘包绕,末梢与其它神经元的胞体形成一处以上相连或不相连的对称型突触,也可与其它树突形成对称型的突触.根据该神经元的形态,递质性质及突触分布的特征,可确认它是篮状细胞.     

散发性戊型肝炎病毒感染的诊断

用基因工程重组的戊型肝炎病毒基因结构区第二码框架和第二读码框架具有免疫表位的嵌合抗原，建立了间接酶联免疫法，检测散发性急性肝炎病人血清中抗－ＨＥＶＩｇＧ和ＩｇＭ抗体。在４６例急性肝炎病人中出抗－ＨＥＶＩｇＧ抗体阳性７例，阳性率为１５．２２％，７例ＩｇＧ抗体阳性中，有５例ＩｇＭ抗体也阳性，占７１．４％。     

Frequent rearrangement of chromosomal bands 1p22 and 11q13 in squamous cell carcinomas of the head and neck

We report the finding of clonal structural chromosome abnormalities in short-term cultures from 15 squamous cell carcinomas of the head and neck region. When the distribution of chromosomal breakpoints in these 15 tumors and in the 16 head and neck carcinomas previously described are assessed, a marked clustering is seen at bands 1p22 and 11q13, which are rearranged in eight and nine tumors, respectively. No other band was involved in aberrations in more than five tumors. Cytogenetic evidence of gene amplification was seen in four tumors, three times in the form of homogeneously staining regions (twice located in 11q13), and in one tumor as double minutes. Among the candidate genes for such amplification are BCLI, INT2, and HSTI, all of which map to 11q13, and NRAS, which maps to 1p22. All these oncogenes have previously been shown to be amplified in subsets of head and neck carcinomas. We conclude that bands 1p22 and 11q13 are nonrandomly involved in chromosomal rearrangements in head and neck carcinomas and suggest that activation of oncogenes located in these bands may proceed via cytogenetic mechanisms.     

注意缺陷多动障碍儿童罗夏测验的反应特征分析

目的:探讨注意缺陷多动障碍(Attention deficit hyperactivity disorder,ADHD)儿童在罗夏测验中的表现特征及可能的投射机制。方法:采用国内修订版罗夏测验(Rorschach Inkblot Test,RIT),结合艾森克人格问卷(EPQ)对40名ADHD男童和40名对照组儿童进行了测试。结果:ADHD儿童在RIT变量Zf、COP、3r 2/R、Zd、X %、Sum6的得分低于对照组(P<0.05);而在变量AG、Lambda、Sh、X-%、SCZI、DEPI、CDI的得分高于对照组(P<0.05);两组儿童在RIT多个变量和EPQ各分量表之间存在相关。结论:ADHD儿童在RIT中表现出较多的在客体关系、情绪特质、自我功能和认知功能方面的缺陷,说明RIT可投射出ADHD儿童内心世界的一些特点,包括自我意识不良、人际关系不良、攻击性、情绪问题、认知过程和思维能力问题。     

系统性红斑狼疮患者脱氧核糖核酸酶1基因表达研究

目的　研究脱氧核糖核酸酶 1(deoxyribonuclease ,DNASE1)基因表达及 m RNA剪接形式与系统性红斑狼疮 (systemic lupus erythematosus,SL E)的关联性。　方法　以实时荧光定量聚合酶链反应 (real- time PCR)方法检测 DNASE1的 m RNA表达水平 ,以毛细管电泳技术分析 m RNA编码区替代剪接体 ,结合单核苷酸多态性 (single nucleotide polymorphisms,SNPs)单倍型了解基因结构对表达的影响。　结果　SL E患者 DNASE1基因表达水平显著高于正常对照 (P<0 .0 0 1) ,未发现 SL E疾病活动性指数积分与基因表达水平存在相关性 ,但性别分析显示女性患者 DNASE1基因表达水平高于男性患者 (P<0 .0 1)。 8名正常人与 18例患者的毛细管电泳结果显示 ,患者与正常人替代剪接谱系不同 ,且 380 bp处存在明显条带 ,具有不同单倍型的 SL E患者替代剪接谱系亦有差异。　结论　 SL E患者 DNASE1基因表达异常 ,并存在与正常人不同的 m RNA剪接体。 DNASE1基因与 SL E发病相关     

大鼠心脏穿刺采血方法的改进

目的:介绍一种大鼠活体重复采血方法。方法:大鼠腹腔麻醉后,仰卧位沿剑突下斜行向上刺入2.5~3.0cm入心,抽血。60只大鼠,每间隔10天采血2.0ml,共4次。测定其Hb、RBC、WBC、Hct、ALT、AST、TP、BUN等指标,同时心脏切片行病理学检查。结果:采用本法进行大鼠心脏取血,成功率97.8%。各次标本的实验室指标均无显著性差异(P>0.05)。20只大鼠第4次采血后剖胸,未见胸腔脏器出血、粘连、血肿等并发症。心脏切片未见心肌组织学损伤。结论:该技术操作简便,定位准确,可重复采血,动物存活率高。尤其用于自身对照,可减少动物用量,提高实验准确性。