窒息新生儿出生体重与血清同型半胱氨酸关系

目的探讨窒息新生儿出生体重对其血清中高同型半胱氨酸血症及低叶酸水平的关系。方法同期出生的新生儿分为正常组和窒息组,并分别分为正常体重及低体重组。应用酶联免疫吸附实验方法检测血清中Hcy水平。应用放射免疫法测定血中叶酸浓度。结果窒息组正常体重儿血清同型半胱氨酸为(10.50±2.19)μmol/L,低体重儿为(15.82±2.51)μmol/L,对照组正常体重儿血清同型半胱氨酸为(7.73±0.61)μmol/L,低体重儿为(7.45±0.43)μmol/L。在窒息组内低体重儿血清同型半胱氨酸水平明显高于正常体重儿(P<0.001),且窒息低体重组儿血清同型半胱氨酸水平明显高于正常对照组儿,不论其出生体重如何(P<0.001)。血清叶酸水平检测结果显示窒息组正常体重儿血清叶酸水平为(2.74±0.16)μg/L,低体重儿为(2.29±0.25)μg/L,对照组正常体重儿血清叶酸为(3.33±0.23)μg/L,低体重儿为(3.02±0.29)μg/L。窒息组患儿无论正常体重儿还是低体重儿其血清叶酸水平变化均不显著(P>0.05)。结论缺氧窒息合并低出生体重者血清同型半胱氨酸水平显著升高。     

香砂六君颗粒防治脾虚胃病的动物实验研究

目的 探讨香砂六君颗粒对脾虚胃病的防治机理。方法 采用酸性乙醇反复损伤，复金用饥饱失常、劳倦过度方法造成脾虚症和胃粘膜损伤的动物模型，并用香砂六君颗粒防治，观察胃粘膜屏障、免疫、胃肠运动等多项指标。结果 香砂六君颗粒有对抗胃粘膜上皮损伤，增加胃粘膜磷脂含量和胃粘膜粘液量；使外周血和胃粘膜CD4计数上升，CD8计数降低，CD4／CD8比值升高；升高MTL、GAS水平，降低SS水平，促进胃排空和降低小肠推进比。结论 香砂六君颗粒有保护胃粘膜屏障、调节免疫和胃肠运动功能的作用。     

量化健康教育在CCU住院病人健康教育中的应用

医院健康教育是护理人员通过各种教育形式使病人或家属获得预防疾病、促进康复、保持健康知识的护理活动[1]。它有多种教育形式,本文就量化健康教育与随机健康教育效果进行比较。1对象与方法1.1对象2002年1月—2004年1月,知道自己所患病情,能清楚表达自己意愿的200例CCU住院病人为对象。按随机数字表将200例病人分为实验组和对照组。实验组男68例,女40例,年龄(58.7±6.9)岁;初中及以下35例,高中及以上73例;高血压36例,急性心肌梗死13例,不稳定性心绞痛17例,恶性心律失常27例(行起搏器植入术7例),急性左心衰竭15例。对照组男60例,女32例,年…     

异位妊娠的腹腔镜手术临床分析

目的探讨电视腹腔镜手术治疗内出血异位妊娠的安全性和可行性。方法回顾分析2000年8月￣2004年8月该院电视腹腔镜手术治疗的15例伴有内出血的异位妊娠(出血量大于800mL,观察组)及同期30例无明显内出血异位妊娠病人的临床资料(对照组)。结果15例病人均手术成功,无中转开腹及并发症发生,两组手术时间及手术后住院时间均无明显差异。结论随着腹腔镜手术操作技巧、麻醉和监护技术的提高,腹腔镜手术治疗内出血甚至休克型异位妊娠在一定条件下是安全可行的。     

Ki-67抗原与膀胱癌相关性研究进展

<正>膀胱癌是我国泌尿外科肿瘤发病率和病死率均占第一位的肿瘤,约80％为浅表性移行细胞癌。但是,目前常规的病理学检查无法为临床提供指导性的治疗意见。细胞增殖在膀胱癌的发生、发展过程中起到重要作用。检测膀胱癌增殖活性对于判断其恶性程度、预见其生物学行为和选择适当的治疗方案具有重大意义。Kj-67抗原(简:Kj-67)是近年来受     

Lipid extract from completely sporoderm‐broken germinating Ganoderma sinensis spores elicits potent antitumor immune responses in human macrophages

Ganoderma sinensis has been used widely in Oriental countries for the prevention and treatment of various diseases including cancer. Previous studies have shown that the lipid extract from Ganoderma exhibits direct cytotoxicity against tumor cells. Here, it is reported that the lipid extract from germinating G. sinensis spores, at lower concentrations that have no direct tumoricidal activity, induce potent antitumor immune responses in human monocytes/macrophages. Upon stimulation with the lipid extract, monocytes/macrophages exhibited markedly increased production of proinflammatory cytokines and surface expression of costimulatory molecules. Conditioned medium from stimulated cells effectively suppressed the growth of tumor cells. Apparently, the lipid extract triggered macrophage activation via a mechanism different from that associated with LPS. Moreover, it was observed that the lipid extract could partially re‐establish the antitumor activity of the immunosuppressive tumor‐associated macrophages. These results indicated that in addition to its direct tumoricidal activity, the lipid extract from G. sinensis spores could exert antitumor activity by stimulating the activation of human monocytes/macrophages. Copyright © 2008 John Wiley & Sons, Ltd.     

Kindergarten children's representation and understanding of written number symbols

Two samples of kindergarten children's representation and understanding of written number symbols were examined in two time points in one academic year. About 85% of Chinese five year olds (mean = 5 years 10 months) were able to use conventional number symbols to represent the quantity of 30 or larger. At the end of the kindergarten year, 94% of Chinese six year olds (mean = 6 years 4 months) were able to use conventional number symbols to represent the quantity of 100 or larger. Some Chinese six year olds had problems in representing written addition and subtraction tasks. Children's ability to represent written number symbols, ability to represent written addition and subtraction formula and their performance on written addition and subtraction were closely related. The performance of children in a university-affiliated childcare center was better than that of the center serving working-class families in Time 1, but the performance reversed at the end of the kindergarten year.     

经鼻肠管肠内营养在胃癌根治术中的应用

目的探讨经鼻肠管肠内营养(EN)在胃癌根治术患者中的应用效果。方法在72例行胃癌根治术患者中,34例术后行肠外营养(PN)治疗,38例应用鼻肠管于术后早期行肠内营养(EN)治疗。结果术后EN组的血红蛋白和血清蛋白与PN组基本相同,而AKP、GGT和TB明显低于PN组。结论经鼻肠管的EN比PN更适合胃癌根治术患者的营养支持治疗。     

增强子Ⅰ对乙型肝炎病毒DNA疫苗免疫应答的影响

目的研究乙型肝炎病毒(HBV)自身增强子Ⅰ(enhancerⅠ,ENHⅠ)对HBVDNA疫苗免疫应答的影响。方法采用PCR法以HBVadr亚型全基因DNA序列为模板分别扩增表面抗原(HBsAg)和HBsAg-ENHⅠ基因片段,重组到载体VR1012中,构建两种HBVDNA疫苗,转染COS-7细胞及HepG2细胞并免疫BALB/c小鼠。采用蛋白印迹、ELISA、ELISPOT等方法检测其在COS-7和HepG2细胞内的表达及小鼠的体液及细胞免疫应答效果。结果转染的HepG2和COS-7细胞均表达HBsAg;连接ENHⅠ的HBVDNA疫苗转染HepG2细胞后HBsAg表达量明显升高,两种疫苗转染COS-7细胞表达HBsAg无明显差异;免疫小鼠后第2周产生HBsAb及HBsAg特异性细胞毒T淋巴细胞(CTL),两种疫苗免疫产生的HBsAb及HBsAg特异性CTL无明显差异。结论ENHⅠ可使HBVDNA疫苗转染HepG2细胞表达HBsAg明显增加,对转染COS-7细胞表达HBsAg及接种BALB/c小鼠引起的免疫应答无明显影响。     

HPLC法测定欣康胶囊中次乌头碱含量

目的测定欣康胶囊中次乌头碱的含量。方法采用反相高效液相法,以C18为固定相,甲醇-水-三乙胺(75∶25∶0.05)为流动相,检测波长为232nm。结果平均回收率为94.65%,RSD为3.54%。结论方法简便,准确。