建立中医方剂数据挖掘系统的探讨

以历代方剂作为数据库的主要信息来源,对原始资料进行标准规范处理,建立中医历代方剂数据库;根据方药与病证相关的原理,围绕方/药群/药味-病/证/症的信息点,设计不同维度和层次的方药病证检索策略;运用数据挖掘技术,对历代方剂进行方证关联、类方系统、配伍药群、医家处方特征等的研究,以发现证法方药的规律和获得其新知识,最终实现构建资源共享的中医方药数据库及其数据挖掘的方剂信息研究平台。     

中医生精补血抗衰作用的实验探讨

为探讨中医精气血神理论在抗衰老中的实践意义，对体现填精益气补血宁神治法的生精补血汤的抗衰作用进行实验研究。观察到生精补血汤不同剂量能延长果蝇平均寿命，中、高剂量能增加最高寿命；显著提高果蝇产仔数；能显著降低小鼠血浆LPO和提高红细胞GSH水平；能显著抑制体外大鼠肝匀浆LPO生成。提示生精补血汤有抗衰效用和抗氧化剂作用。     

试论证候与复方的研究思路

在21世纪生命科学迅猛发展和回归自然的世界潮流中,中国传统医学的突破,有可能成为中华民族对整个人类的新的重大贡献。辨证论治是中医学中最具特色的部分,而理法方药是辨证论治原则     

中医周期疗法治疗不孕症用药规律分析

不孕症是当今社会不可忽视的问题．其发病率高，已成为世界性共同关注的疑难病证。世界卫生组织（WHO）曾宣布不孕症与心血管疾病、肿瘤列为当今影响人类生活和健康的3大主要疾病。统计资料显示全世界目前有不孕症患者约5000～8000万人，发达国家发病率约为5％～8％．发展中国家一些地区患病率可高达30％。WHO规定。男女双方若无不愿生育的愿望，同居1年以上．有正常的性生活且未采取避孕措施．仍未受孕者．称不孕症（infertility），目前我国多数学者仍主张以2年为标准。     

中药辅助米非司酮终止早孕的疗效观察

中药辅助米非司酮终止早孕的疗效观察谢鸣赵荣胜卞宜心杨卫星有报道，中药生化汤可提高米非司酮配伍前列腺素药物流产（药流）的完全流产率和减少其出血天数［１，２］。为进一步探讨中药提高药流疗效的可能性，我们对中药加味生化膏和安宫冲剂的临床疗效进行比较观察。一...     

柴胡-黄芩药对保肝作用有效部位的研究

目的探查柴胡-黄芩药对保肝作用的有效部位。方法采用乙醇沉淀法,将柴胡-黄芩药对水提液分为A、B、C、D四个部位,以血清ALT、AST为指标,观察柴胡-黄芩药对水提液及其分离部位合并液对CCl4急性肝损伤的保护作用;采用正交试验考察A、B、C、D各部位的保肝效应,最后对分析所得的最佳配伍进行效用再验证。结果柴胡-黄芩药对水提液及其分离部位合并液均能明显降低急性CCl4肝损伤模型大鼠血清ALT与AST（P〈0.05或P〈0.01）;正交试验表明,C和D对模型大鼠血清ALT与AST均有不同程度地降低作用（P〈0.05或接近0.05）,其最佳部位配伍为C＋D;重复验证实验表明,C＋D组能显著降低模型大鼠血清ALT与AST（P〈0.05）,其效应与柴胡-黄芩药对水提液相当。结论部位C、D是柴胡-黄芩药对保肝作用的主要部位。     

23例慢性压迫性颈脊髓病手术治疗的回顾研究

目的 探讨慢性压迫性颈脊髓病压迫程度及MR表现与术后功能恢复的相互关系。方法 选择三年中通过认真减压治疗的颈脊髓病23例,再经过1～3年的临床随访及MR复查。采用JOA评分法进行功能评价,并对MR图像进行测量与观察。结果 减压前、后椎管截面积分别为59.4mm~2和103mm~2;脊髓截面积分别为50.8mm~2和81.48mm~2;脊髓矢状径分别为5.6mm和 7.79mm;JOA评分分别为 6.5分和 9分。以上指标手术前、后比较均有显著性差异(P<0.001)。结论 慢性压迫性颈脊髓病通过手术减压可明显改善脊髓形态,同时改善脊髓功能。     

中医临证组方思路及其评析

问题提出 辨证施治是中医学中最具特色的部分。辨证施治的原则集中体现在临证过程中证法方药的有机统一,即辨明病证、确立相应治法、选择体现此治法的成方,进一步根据病证特点,确定对所选成方进行化裁的必要性:或直接用全方,或增减方中药味、调整剂量,务使方中的全部药味及其相互关系与病证(主要是病机)高度吻合。这是中医临证最基本,也是最重要的     

体外诱导骨髓间充质干细胞向肾小管上皮样细胞分化

Objective To investigate the differentiation of rat bone marrow mesenchymal stem cells (MSCs) to renal tubular epithelial-like cells under different conditions. Methods MSCs were obtained from rat marrow. MSCs were isolated by gradient density centrifugation and plastic adherence and then purified. Surface markers were identified with flow cytometry after amplification in vitro. The purified MSCs of the third passage were cultured respectively as follows: (1) control group: DMEM medium with fetal bovine serum(FBS). (2) all-trans retinoic acid (ATRA) group: DMEM medium with FBS, ATRA and ischemic reperfusion-injured kidney tissue homogenate. (3)combination group: DMEM medium with FBS, ATRA, ischemic reperfusion-injured kidney tissue homogenate, epidermal growth factor (EGF) and bone morphogenetic protein 7 (BMP-7). After 7 days, the MSCs were collected for alkaline phosphatase (AKP) staining, cytokeratin-18 and E-cadherin immunocytochemical analysis. Results The positive rates of the third passage MSCs in CD44, CD90 and CD29 were 97.8%±0.9%, 96.8%±1.4% and 97.6%±2.4%,respectively, but in CD11b/c and CD34 were only 13.2%±0.6% and 1.2%±0.5%. The MSCs in control group were spindle. The MSCs in ATRA group were round and elliptic. The MSCs in combination group became cobblestone-like cells after 7 days. AKP staining showed that tubular epithelial-like cells from MSCs in control group were negative, some above cells in ATRA group were positive and number of above cells increased in combination group. Compared with negative control group, the ratios of cytokeratin-18 positive cells in ATRA group and combination group were respectively increassed by 29.47%±1.08% and 47.52%±2.13% (all P<0.05), the ratios of E-cadherin positive cells in ATRA group and combination group were respectively increased by 14.88%±2.46% and 36.15%±1.13% (all P<0.05). Conclusion MSCs may differentiate by renal tubular epithelial-like cells under the induction of ischemic reperfusion-injured kidney tissue homogenate and ATRA in vitro, which are further differentiated under the combined induction of EGF and BMP-7.