Synergistic Anti-inflammatory Effect of Radix Platycodon in Combination with Herbs for Cleaning-heat and Detoxification and Its Mechanism

Objective:To investigate the synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism for Fei(肺)-targeting.Methods: Forty Wistar rats were randomly divided into five groups(8 per group):the sham-operated group,model group, Radix Platycodon group,Flos Lonicera and Fructus Forsythia(LF) group,and Radix Platycodon,Flos Lonicera and Fructus Forsythia combination(PLF) group,using a random number table.A rat chronic obstructive pulmonary disease(COPD) model was established by passive smoking and intratracheal instillation of lipopolysaccharide(LPS).The treatments started from the 15th day of passive smoking for a total duration of 14 days.At the end of the treatment,changes in the following measurements were determined:lung histopathology, inflammatory cytokines including tumor necrosis factorα(TNF-α),transforming growth factorβ(TGF-β) and interleukin IL-1β(IL-1β) in bronchoalveolar lavage fluid(BALF),and mRNA expression of endogenous active substance intestinal trefoil factor 3(TFF3) in the lung tissue.Results:Light microscopy showed that compared with the sham-operated group,rats in the COPD model group had disrupted alveolar structure,collapsed local alveoli,significantly widened or even fused alveolar septa,and massive infiltration of inflammatory cells in the alveolar wall and interstitium.In addition,significant bronchial epithelium hyperplasia,partially shed epithelia, and marked inflammatory cell infiltration in the bronchial wall and its surrounding tissues were noticed.Electron microscopy showed that rats in the model group had degeneration of alveolar type II epithelial cell;reduction, breakage or even loss of cell surface microvilli;swollen mitochondria with disappearing cristae and vacuole-like structure;and,increased secondary lysosomes in alveolar macrophages.The TNF-α,TGF-βand IL-1βlevels and white blood cell(WBC) count in BALF were significantly increased(P<0.01 or P<0.05) and TFF3 mRNA expression in the lung tissue was significantly reduced(P<0.01).After treatment,the pathological morphology of lung injury was less severe in all three treatment groups.In addition,TGF-βand IL-1βand WBC count in BALF were decreased(P<0.01 or P<0.05),and TFF3 mRNA expression in the lung tissue was significantly increased in the PLF group(P<0.01).Compared with the LF group,the IL-1 p in BALF was significantly decreased (P<0.05),and TFF3 mRNA expression was significantly increased(P<0.05) in the PLF group.Conclusions:Radix Platycodon synergizes with herbs for cleaning-heat and detoxification in reducing inflammatory injury in a rat model of COPD.The synergistic anti-inflammatory effect is reflected in the improvement in pathological changes and in the reduction of IL-1 p levels in BALF.The mechanism of such synergistic action may be related to its effect on maintaining the TFF3 mRNA expression and Fei-targeting function.     

弥可保治疗糖尿病缺血性视神经病变的效果

目的评价弥可保治疗糖尿病缺血性视神经病变的疗效和安全性。方法将42例（60眼）糖尿病缺血性视神经病变病人随机分为两组。治疗组20例（30眼）采用弥可保500μg联合100 mg维生素B1肌肉注射,每天1次,10次为1个疗程,3个疗程后改为口服弥可保500μg和100 mg维生素B1,连用30 d;对照组22例（30眼）采用500μg维生素B12联合100 mg维生素B1肌肉注射,每天1次,10次为1个疗程,3个疗程后改为口服腺苷钴胺片500μg和100 mg维生素B1,连用30 d。观察治疗前后视力、眼底及视野变化。结果治疗组疗效明显优于对照组（u=3.716,P〈0.01）。结论弥可保治疗糖尿病缺血性视神经病变是一种安全、有效的方法。     

妊娠高血压综合征致皮质盲1例

患者女,22岁。因停经39周,下腹阵痛7 h,伴头痛,眼花2 h入院。既往病史:18岁,足月,顺产1女婴,已死亡。入院查体:体温36.0℃,脉搏72次/分,呼吸20次/分,血压170/110 mm Hg。妇科检查:符合正常,妇产科诊断为妊娠高血压综合征(子痫前期重度)。入院后行人工破膜,会阴侧切术,顺娩1女婴,给予解痉,抗炎治疗。     

中药寒热属性的现代研究进展

中药寒热属性主要是指从药物作用于人体所获得的不同疗效总结出来的用药理论,因此认为凡是能减轻或消除热证的药物,一般属于寒性或凉性,凡是能减轻或消除寒证的药物,一般属于热性或温性[1]。中药寒热属性是中医辨证论治、     

两种β-环糊精对苯环壬酯手性识别的串联四极杆质谱研究

目的研究β-环糊精和2,3,6-三甲氧基-β-环糊精对手性药物苯环壬酯的立体选择性识别作用。方法分别将苯环壬酯与两种β-环糊精等体积混合形成包合物后直接进样,采用串联四级杆质谱法,在正离子检测方式下,分析其相对丰度,观察结合性,分析两种环糊精对手性分子苯环壬酯的立体识别能力。结果β-环糊精对苯环壬酯的手性识别作用不明显;2,3,6-三甲氧基-β-环糊精对苯环壬酯对映异构体具有很强的立体识别能力。结论 2,3,6-三甲氧基-β-环糊精与苯环壬酯之间具有显著的立体选择性相互作用。     

“从肠论治”对慢性阻塞性肺疾病模型大鼠肺组织病理学影响

目的:建立慢性阻塞性肺疾病(COPD)模型,观察"从肠论治"对COPD模型大鼠肺组织病理结构变化,寻找中医"从肠论治"肺病理论的形态学证据。方法:采用气管注脂多糖加熏香烟联合造模方法建立COPD大鼠模型,随机分为正常组、模型组、治肺组、治肠组及肺肠同治组。正常组、模型组灌胃生理盐水,各给药组灌胃相应中药,连续14天。采用常规光镜、电镜方法比较各组肺组织病理学变化。结果:与正常组相比,模型组具备COPD的病理特征。与模型组比较,治肠组肺组织病变有所减轻。与治肺组比较,肺肠同治组的病变减轻最明显。结论:通利大肠,或在治肺的基础上增加通利大肠,均能减轻COPD肺组织损伤,"从肠论治"COPD存在形态学证据。     

《伤寒论》“阳微结”历代文献梳理及证治探讨

"阳微结"一词始见于《伤寒论》第148条,多数医家认为该条文中的"阳微结"一词是指阳明腑结之微或指阳气衰微而结,并将其中"结"字解释为大便秘结,然而,笔者认为"结"的含义在"阳微结"之中当有狭义和广义之分,狭义当指"大便秘结",而广义上则有胆气郁结之意义,以此再来审视"阳微结"之涵义,其本质当是少阳经气郁结,胆气不疏。     

急性中心性浆液性脉络膜视网膜病变三维光相干断层扫描图像特征

中心性浆液性脉络膜视网膜病变(CSC)是常见的眼底病变,荧光素眼底血管造影(FFA)可观察到CSC视网膜色素上皮(RPE)水平的"墨渍"状,"炊烟"状等不同状态的渗漏[1].二维光相干断层扫描(OCT)可显示CSC视网膜神经上皮层和RPE脱离反光带等视网膜横截面图像特征[2],但二维OCT只能反映单一视网膜切面,无法提供整个视网膜神经上皮层脱离区域的RPE脱离状况.     

急性中心性浆液性脉络膜视网膜病变三维光相干断层扫描图像特征

中心性浆液性脉络膜视网膜病变(CSC)是常见的眼底病变,荧光素眼底血管造影(FFA)可观察到CSC视网膜色素上皮(RPE)水平的"墨渍"状,"炊烟"状等不同状态的渗漏[1].二维光相干断层扫描(OCT)可显示CSC视网膜神经上皮层和RPE脱离反光带等视网膜横截面图像特征[2],但二维OCT只能反映单一视网膜切面,无法提供整个视网膜神经上皮层脱离区域的RPE脱离状况.