除草剂丁草胺的致突变性研究

用微核试验,骨髓细胞和睾丸生殖细胞染色体畸变分析等方法对丁草胺的致突变性进行了测定,结果全部试验均为阴性,提出丁草胺没有诱变活性,它可能是一种没有明显遗传危害作用的除草剂。     

Chicken Embryonic Femur Cultued in Vitro

ＣｈｉｃｋｅｎＥｍｂｒｙｏｎｉｃＦｅｍｕｒＣｕｌｔｕｒｅｄｉｎＶｉｔｒｏＣｈｉｃｋｅｎＥｍｂｒｙｏｎｉｃＦｅｍｕｒＣｕｌｔｕｒｅｄｉｎＶｉｔｒｏＸＵＲｏｎｇ－ｈｕｉ（徐荣辉）；ＺＨＵＹａ－ｐｉｎｓ（朱雅萍）；ＣＨＡＩＢｅｎ－ｆｕ（柴本甫）（Ｓｈａｎｇ...     

丹参注射液对鸡胚额骨分离细胞培养生长影响的组织化…

实验用鸡胚额骨分离细胞体外培养结合组织化学技术观察丹参注射液对成骨细胞样细胞增殖、分化和成骨功能的影响。发现：（１）丹参能明显促进成骨细胞样细胞的分裂和增殖。（２）丹参能促进成骨细胞样细胞提前出现伪足伸缩运动和胞外吐作用，使丹参组的培养液内出现大量细小漂浮性颗粒，该颗粒对酸性粘多糖染色呈阳性反应。（３）成骨细胞样细胞经发育后能分泌直径约为３μｍ的小光状颗粒。这种颗粒显酸性粘多糖着色（早期），胶原和     

上海市松江区洞泾镇老年慢性肾脏病筛查评估及并发症现状分析

目的了解上海市松江区洞泾镇老年慢性肾脏病(chronic kidney disease,CKD)筛查评估及并发症现状。方法收集2017—2018年接受老年人免费健康体检的数据和2018年心脑血管疾病监测系统数据。以估算肾小球滤过率(eGFR)60mL×min~(-1)×1.73 m~(-2)定义为CKD患者。对比2017年和2018年eGFR均值和CKD检出率,同时对CKD进展情况进行评估,比较2018年CKD并发症的检出情况。结果 1 850名老年人中2018年CKD的检出率(7.78%)高于2017年(4.38%),2018年eGFR均值(90.76±21.89 mL×min~(-1)×1.73 m~(-2))低于2017年(95.71±21.92 mL×min~(-1)×1.73 m~(-2));男性GFR分期改变的比例高于女性,75岁及以上人群eGFR较基线值下降≥25%的比例和GFR分期改变的比例均高于60～74岁人群;2018年CKD患者、eGFR较基线值下降≥25%和GFR分期改变的人群贫血和高同型半胱氨酸血症的检出率均更高;2018年CKD患者心脑血管疾病的发病率更高,以上对比差异均具有统计学意义(P0.05)。结论高龄人群是社区CKD筛查的重点人群,同时应在社区免费体检项目中增加CKD筛查及其并发症相关指标的检测和监测。     

OSTEOGENIC POTENTIAL OF RABBIT DERMAL FIBROBLASTS CULTURED IN VITRO: A HISTOCHEMICAL AND RADIOAUTO-GRAPHICAL STUDY

ＯＳＴＥＯＧＥＮＩＣＰＯＴＥＮＴＩＡＬＯＦＲＡＢＢＩＴＤＥＲＭＡＬＦＩＢＲＯＢＬＡＳＴＳＣＵＬＴＵＲＥＤＩＮＶＩＴＲＯ：ＡＨＩＳＴＯＣＨＥＭＩＣＡＬＡＮＤＲＡＤＩＯＡＵＴＯ┐ＧＲＡＰＨＩＣＡＬＳＴＵＤＹＣＨＡＩＢｅｎ－ｆｕ（柴本甫），ＴＡＮＧＸｕｅ－...     

EFFECT OF RADIX SALVIAE MILTIORRHIZAE ON GROWTH OF ISOLATED CELLS FROM EMBRYONIC CHICKEN FRONTAL BONE CULTURED IN VITRO (A HISTOCHEMICAL STUDY) Ⅱ.THE DEVELOPMENT AND MATURATION OF OSTEOBLAST-LIKE CELLS

The isolated osteoblast-like cells from embryonic chicken frontal bone werecultured in vitro and histochemical methods adopted to observe the effect of RadixSalviac Miltiorrhizae (RSM) on proliferation, differentiation, and osteogenic capacity ofthese cells. It was found that: 1. The mitosis and proliferation of the osteoblast-like cellscould be accelerated by RSM, resulting in increased density of the cells in RSM groupas compared with the control. 2. After 48 h, the pseudopodia stretched out and drew backactively in osteoblast-like cells in RSM group. Small particles produced in the cells weresecreted through exocytosis to the extracellular medium. However, in the control group,the capacity to form and secrete these particles was limited. These particles showed posi-tive Alcian blue staining in Alcian blue-Sirius red reaction, so they were acidmucopolysaccharide particles. 3. The osteoblast-like cells could secrete vesicular particles 3micra in diameter. These vesicular particles could be stained with Alcian blue in earlystage, then they could be stained with Sirius red, and finally by Alizarin red S. Thesevesicular particles could aggregate and fuse around the cell colonies, forming bonenodules and bone flakes. The quantity and volume of the bone nodules and flakes inRSM group were larger than in the control group. 4. The bone nodules and flakes couldbe labeled vitally with tetracycline, and show strong yellow fluorescence under thefluorescence microscope. Therefore, these substances were the newly formed bone sub-stances.     

丹参对家兔桡骨骨折愈合的影响

48只家兔在左前臂旋前圆肌肌止远端造成3 mm宽缺损的桡骨标准骨折,随机等分为丹参组与对照组。于术后第3、5、7、9、13、17、21及25天每组各处死家兔3只,制作石蜡标本,经HE染色和picrosirius染色,作可见光和偏振光观察。发现:1.在炎性反应阶段丹参使扩张充血的毛细血管提前出现,使创伤局部血供提前得到改善。2.在清扫阶段,丹参组血肿内含铁血黄素较对照组提前出现,说明清扫提前进行。3.在细胞活动以及纤维骨骨痂、原始骨性骨痂、继发骨性骨痂的出现时间方面丹参组均比对照组有提前。说明通过丹参的活血化瘀作用,使骨折愈合的各个阶段都较对照组提前,从而促进了骨折愈合。     

SCANNING ELECTRON MICROSCOPIC STUDY OF FETAL CHICKEN CALVARIAL OSTEOBLAST-LIKE CELLS CULTURED IN VITRO

Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. With passage of culture time, there werechanges in configuration so that the spindle-shaped cells and the globular cells turnedgradually into squamous cells, in quantity which increased greatly to produce confluenceand multi-layer formation of cells, and in function as evidenced by emergence ofintracytoplasmic granules, reflecting collagen synthesis.