冠心病患者血中白介素-1β、肿瘤坏死因子α和血脂含量变化及意义

目的 :探讨白介素 1β(IL 1β)、肿瘤坏死因子α(TNF α)与血脂的关系及其在冠心病 (CHD)发病机制中的作用。方法 :对 6 4例患者行冠状动脉造影 ,其中CHD组 33例 ,非CHD组 31例。测定两组血清中总胆固醇(TC)、甘油三酯 (TG)、高密度脂蛋白胆固醇 (HDL C) ,IL 1β和TNF α。 结果 :CHD组血TC、TG、IL 1β、TNF α显著高于非CHD组 (P <0 .0 1) ,而HDL C显著低于非CHD(P <0 .0 1) ;CHD组TNF α与TC呈显著正相关 (r =0 .86 2 ,P <0 .0 1) ,非CHD组TNF α与TC不相关。结论 :IL 1β、TNF α与血脂异常之间存在一定联系 ,三者可能均参与了CHD发生、发展过程     

证明心脏病与基因有关“心脏病第一家族”功不可没

美国有这样一个不幸的家族：家族中有11人先后患上心脏病，他们有的活了下来，有的被病魔夺走了生命。对于斯特芬森家族来说，每个成员都明白，不幸随时可能降临到自己头上。尽管如此，这个家族还是本着积极乐观的态度，勇敢献身科学实验。     

肺炎衣原体通过上调酰基辅酶A:胆固醇酰基转移酶1诱导人单核细胞株THP-1源性泡沫细胞形成的实验研究

Objective To investigate the expression changes of acyl-coenzyme A: cholesterol acyhransferase 1 (ACAT1) on Chlamydia pneumoniae (C. pn) induced foam cell formation. Methods Human monocytic cell line (THP-1) was induced into macrophages by 160 nmol/L phorbol myristate acetate (PMA)for 48 h, and were randomly allocated into four groups: negative control group (50 μg/ml LDL for 48 h) ; positive control group (50 μg/ml ox-LDL for48 h) ; C. pn infection group (50 μg/ml LDL plus 1× 105,4×105,5×105and 1×106 IFU C.pn for 48 h or 1×106 IFU C.pn for 0,24,48 and 72h); ACAT inhibitor 58-035 plus C. pn infection group (1, 5, 10 μg/ml ACAT inhibitor 58-035 pretreatment for 1 h,50 μg/ml LDL and 1 × 106 IFU C. pn for 48 h). The mRNA and protein expressions of ACATI were determined by RT-PCR and Western blot, respectively. Lipid droplets in cytoplasm were observed by oil red 0 staining. The contents of intracellular cholesteryl esters were detected by enzyme-fluorescence. Results The mRNA and protein expressions of ACATI were significantly up-regulated in positive control cells compared those in negative control cells and further upregulated by C. pn infection in a time-dependent and concentration-dependent manner (all P ＜ 0.05). There were significantly increases in the accumulation of lipid droplets and the ratio of cholesteryl ester to total cholesterol in positive control cells as compared with negative control cells and these were further aggravated by C. pn (at the concentrations of 5× 105 and 1×106IFU for 48 h) and C. pn infection induced increases in the accumulation of lipid droplets and the ratio of cholesteryl ester to total cholesterol could he significantly attenuated by ACAT inhibitor 58-035 (all P ＜ 0.05). Conclusion Chlamydia pneumoniae induces THP-1-derived foam cell formation by up-regulating the expression of ACATI.     

肺癌患者单个核细胞产生白细胞介素－1能力的初步研究

肺癌患者单个核细胞产生白细胞介素－１能力的初步研究焦光宇何平马宗生张晓晔１．对象：４０例肺癌、１１例胸部良性疾病均为我院住院患者，３０名正常对照者为健康体检者。肺癌患者均经病理证实，其中鳞癌１９例、腺癌１４例、小细胞癌５例；ＴＮＭ分期：Ⅰ期１４例，Ⅱ...     

静脉用右旋糖酐氢氧化铁注射液治疗肾性贫血疗效观察

目的观察大剂量静脉给予右旋糖酐氢氧化铁注射液(科莫非)治疗慢性肾衰患者铁缺乏的有效性与安全性。方法对2004-01~2005-02中国医科大学第二临床学院肾内科46例患者根据公式计算总补铁量,应用科莫非进行补铁治疗。于用药前及首次用药后2周、停药后2周检测血红蛋白(Hb)、红细胞压积(HCT)、红细胞(RBC)、网织红细胞(Ret)、血清铁蛋白(SF)及转铁蛋白饱和度(TSAT)等指标,同时观察有无不良反应。结果(1)46例患者中铁贮存低下者18例,占40%;相对铁缺乏者28例,占60%。(2)首剂用药后2周及总量应用结束停药后2周与用药前相比,Hb、HCT、RBC、Ret、SF及TSAT均显著升高,P<0.05;总量应用结束停药后2周Hb、HCT、RBC、SF及TSAT与首剂用药后2周相比进一步升高,P<0.05,而Ret稍下降,P>0.05,差异无统计学意义。(3)46例中显效26例,有效17例,总有效率93.5%,无效0例。无不良反应发生。结论静脉给予大剂量右旋糖酐氢氧化铁注射液治疗慢性肾衰患者的铁缺乏,疗效确切,方便可行,起效较快,未发生明确的副反应。     

2007年贵州省从江县碘缺乏病健康教育效果评价

Objective To evaluate the effect of health education in controlling the iodine deficiency diserders(IDD) in order to provide reference data for the further prevention and control. Methods Each village of 3 towns in Congjiang County was selected in 2007, where the health education lasting for 10 months had been implemented in the school students of 3-6 grade and the villagers. The school students of 3-6 grade and 30 housewives in the villagers were investigated for their IDD control knowledge, the salt consuming conditions as well as the sales of both rough and fine salt at a salt retail site in each village before and after the health education was implemented. Results The awareness rate of the knowledge of IDD control in the students and housewives was 91.4% (581/636) and 78.3% (282/360), respectively after intervention, which significantly increased (χ2= 532.044, 326.117, both P < 0.01) compared with the rate of 28.2% (184/652) and 11.4% (41/360) before intervention. The proportion of consuming fine salt was 91.8%(146/159) and 95.6%(86/90), significantly inereased(χ2= 236.623, 135.350, both P < 0.01) compared with 6.1%(10/163) and 7.8% (7/90) found before intervention. The selling proportion of fine salt at the salt retail site in the village was 60.0%(900/1500), significantly increased(χ2= 824.176, P < 0.01) compared with 10.0%(150/1500) before intervention. Conclusions Health education and promotion is solid foundation for effectively controlling IDD, through which the students and villagers are actively and voluntarily involved in the program and hence have formed good living and hygiene habits, thus expected effect has been obtained.     

贵州省从江县翠里村精神与智力障碍病例调查

目的了解贵州省从江县翠里村“精神与智力障碍”人群发病情况,为确诊与病因调查提供线索。方法对“精神与智力障碍”人群进行体格检查及流行病学个案调查,对目标人群(育龄妇女和8～10岁儿童)进行尿碘、甲状腺肿大率、智商以及居民家中食盐含碘量检测。结果病人家族中无近亲婚史和有关疾病家族史,无遗传、家庭聚集现象,人群分布主要是男性,年龄分布主要在20岁以上人群,22人中有21人具有克汀病病人表现,8～10岁儿童甲状腺肿大率(甲肿率)5.88%,尿碘中位数184.9μg/L,智商值(IQ)均数86.8;育龄妇女尿碘中位数174.6μg/L,盐碘均值23.9mg/kg。结论该村目前无克汀病发病的基础,该村34例“精神与智力障碍”病人有可能是过去发病的克汀病病人。     

代谢综合征大鼠中血清瘦素和肿瘤坏死因子α与胰岛素抵抗的关系

目的探讨瘦素与肿瘤坏死因子-α(TNF-α)在代谢综合征的发生发展过程中扮演的角色.方法高糖高脂饮食喂养SD大鼠12周后分别测血糖、血脂、血清胰岛素的水平,胰岛素抵抗(IR)的程度用稳态模型(Homa-IR)的IR指数(IAI)评估,血清中瘦素的水平用放免法测定TNF-α用ELISA方法检测,另取大鼠腹内脂肪组织,用免疫组化的方法观测脂肪细胞中瘦素表达的情况.结果高糖高脂饮食喂养12周的大鼠体重、空腹血糖、血脂以及空腹血清胰岛素的水平均高于正常对照组(P＜0.01),IAI与正常对照组相比有显著性升高[(4.44±0.27)∶(5.45±0.51),P＜0.05],血清瘦素和TNF-α与正常对照组相比也有明显升高[(0.15±0.05)∶(0.41±0.23),P＜0.01].结论高糖高脂饮食可诱导大鼠代谢综合征模型的建立,IR与血清瘦素和TNF-α的关系密切,瘦素和TNF-α的水平升高可作为代谢综合征的临床特征之一,以此通过代谢综合征的发展情况预测心血管疾病的发生和发展.     

贵州省未达到消除碘缺乏病目标地区的流行现状调查

目的了解贵州省碘缺乏病(IDD)防治现状。方法按国家IDD监测方案,抽取贵州省5个地区11个县对学校儿童甲状腺肿大率、尿碘及碘盐等进行横断面调查。结果8～10岁学生平均甲状腺肿大率9.66%,最低2.8%,最高15.78%,年龄组间差异有显著意义,居民户盐碘中位数32mg/kg,碘盐覆盖率99.47%,合格率92.11%,儿童尿碘中位数263.9μg/L,其中<100μg/L占6.1%,<300μg/L占52.5%,≥300μg/L占41.31%。结论贵州省碘盐覆盖率、合格率较往年明显上升,儿童尿碘水平趋于更为合理,但8～10岁学生甲状腺肿大率仍处于较高水平。     

Fas/FasL途径在氟致人神经母细胞瘤SH-SY5Y细胞凋亡中的作用

目的 探讨Fas/FasL途径在氟致人神经母细胞瘤SH-SY5Y细胞凋亡中的作用.方法 用不同剂量氟化钠[NaF,0(对照)、20、40、80 mg/L].SH-SY5Y细胞进行染毒,24 h后检测细胞存活率、凋亡率和Fas、FasL mRNA表达;选择40 mg/L NaF组,观察在Fas受体激动剂(CH11)或拮抗剂(ZB4)作用下细胞凋亡率及Fas、FasL mRNA表达水平的改变.结果 40、80 mg/L组细胞存活率[(84.63±2.57)%、(69.04±5.63)%]明显低于对照组(100.00%),组间比较差异有统计学意义(P<0.01);细胞凋亡率随染毒剂量的升高呈上升趋势.40、80 mg/L组细胞凋亡率[(8.54±1.95)%、(17.94±2.71)%]明显高于对照组[(3.32±1.33)%],组间比较差异有统计学意义(Jp<0.05);NaF能不同程度地上调Fas、FasL mRNA表达,使Fas/β-actin[40mg/L组(0.94±0.51)、80 mg/L组(0.99±0.12)]和FasL/β-actin[40 mg/L组(0.96±0.42)、80 mg/L组(0.99±0.24)]比值增加.与对照组[Fas/β-actin(0.50±0.33)、FasL/β-actin(0.58±0.23)]比较,差异均有统计学意义(P<0.05).在对细胞凋亡率和Fas、FasL mRNA表达水平的影响中,NaF与CH11之间存在协同作用(,值分别为32.89、18.46、14.69,P<0.01),NaF与ZB4之间存在拈抗作用(F值分别为5.73、24.26、10.17,P<0.05或<0.01).结论 NaF可诱导SH-SY5Y细胞凋亡,Fas/FasL途径在NaF诱导SH-SY5Y细胞凋亡中起重要作用.