尼龙线皮下潜行缝合法治疗髌骨骨折128例

我院采用尼龙线皮下潜行缝合法治疗髌骨骨折,收到满意疗效。现将资料完整的128例,整理报告如下。临床资料本组128例中男74例,女54例;年龄24～76岁;右膝81个,左膝47个;髌骨骨折类型(均为闭合性):横断型95例,粉碎型33例;骨折块分离情况:原位15例;0.5～2cm58例,2～4cm47例,>4cm8例;膝关节腔内积血情况:<10ml21例,11～40ml52例,>40ml55例。治疗方法1.患者取平卧位,患肢膝部术野常规消毒铺单。不需用止血带。将消好毒的尼龙线分成等长的两段,分别穿入大三角针内备用(注意尼龙线应用非有机类溶液消毒,如用0.5～1‰新洁尔灭液浸泡1小时)。2.在髌骨…     

喉癌细胞增殖能力与放疗疗效的关系

早期喉癌放射治疗目前已经逐渐成为重要的治疗手段,但却有10%～35%的复发率影响着患者的喉保存率及5年生存率[1]。肿瘤细胞增殖能力是影响放疗疗效的一个重要因素,我们应用单克隆抗体ＭＩＢ1对喉癌组织进行了免疫组织化学染色,分析了喉癌细胞增殖能力与放射线治疗疗效的关系,报道如下。一、研究对象和方法1病例选择:选择1985～1995年间诊断为喉鳞癌,并行单纯放射治疗无效的30例喉癌患者,按ＴＮＭ及生长部位可比原则随机选择40例单纯放射治疗有效的喉癌患者。其中Ｔ1期57例,Ｔ2期12例,Ｔ3期1例。喉癌按照1987年国际抗癌联盟(ＵＩＣＣ)喉癌分…     

针刺配合运动疗法结合桂枝葛根汤加减治疗落枕心得

在临床实践过程中,发现针刺配合运动疗法,结合中药辨证施治治疗落枕疗效较好,希望该治疗方法在临床上得到广泛应用,把传统疗法发扬光大,为广大患者解除痛苦。     

IRAK-4在大鼠缺血性脑损伤早期的表达及意义

目的 探讨白细胞介素-1受体相关激酶-4(interleukin-1 receptor-associated kinases,IRAK-4)在大鼠大脑缺血性损伤早期的表达及意义.方法 应用大脑中动脉线栓法(MCAO)建立大鼠局灶永久性脑梗死模型,随机分为正常对照组、假手术组、缺血组、干预组.采用RT-PCR、Western blot、免疫荧光、神经功能5分评定及TTC染色等方法,检测大鼠额顶叶皮层组织IRAK-4在脑缺血性损伤早期的mRNA及蛋白表达变化、神经功能缺损程度及脑梗死体积变化,并统计各组死亡率.结果 大鼠大脑缺血性损伤早期额顶叶皮层组织IRAK-4 mRNA表达:3 h开始增加,12 h达到高峰,24 h仍维持在较高水平;IRAK-4蛋白表达:3 h开始增加,6 h达到高峰,12 h仍维持在较高水平,24 h下降与正常对照组无统计学意义;IRAK-4抑制能明显缩小脑梗死体积、改善神经功能、降低死亡率.结论 大鼠额顶叶皮层组织IRAK-4在脑缺血性损伤早期表达迅速上调,可能参与了脑缺血后炎性反应的调控,早期抑制其功能可以发挥显著脑保护作用.

Abstract：

Objective To investigate the expression and significance of Interleukin - 1 ReceptorAssociated Kinases 4( IRAK -4) in rat ischemic brain injury. Method Models of focal cerebral infarct were established by occlusion of middle cerebral artery ( MCAO ). Rats were randomly divided into normal control group, sham surgery group, ischemic group, treated group. The mRNA and protein expressions of IRAK-4 in cerebral cortex were detected by RT- PCR method, Western blot analysis and laser scanning confocal microscope(LSCM). The neurological deficits were evaluated in 0 ～ 4 scales. The infarct size was measured by TTC staining. The mortality of each group was also calculated. Results The mRNA and protein expressions of IRAK -4 in cerebral cortex increased after ischemic injury 3 hours and decreased gradually after reaching the highest level at 12 h or 6 h( P ＜0.01 ) ,after 24 h the concentration of protein showed no significant differences compared with normal control group ( P ＞ 0.05 ), but mRNA is still at a high level ( P ＜ 0.05 ) ;the inhibition of IRAK -4 could induce the cerebral infarct, neurological deficits and mortality rate. Conclusions Brain ischemic injury increased the expression of IRAK - 4 in cerebral cortex,and inhibition of neuronal IRAK-4 by IRAK- 1/4 inhibitor at the early stage could effectively protect the neuron against death. The mechanism may be related with down - regulation of the inflammation response resulted from stroke.     

党中勤治疗便秘经验

目的:总结党中勤教授治疗便秘的经验。方法:通过跟师学习,分析病例,进行总结。结果:党师指出魄门亦为五脏使,便秘虽属大肠传导失常,但与脾、肺、肝、肾等脏功能失调密切相关,便秘之病不可一概用大黄、芒硝之属,应究其源而辨证论治。结论:党师提出的培中土补益气血、开肺金通腑行便、解肝郁复木疏泄、温肾水开窍后阴等治疗便秘的思路及经验方药,临床疗效显著。     

柴胡皂苷a影响大鼠急性脊髓损伤后机体炎症水平表达的实验研究

Objective: To investigate the effect of saikosaponina on the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the early stage of acute spinal cord injury in rats. Methods: Thirty SD rats were randomly divided into sham operation group, simple injury group, saikosaponin a 5 mg / kg group, 10 mg / kg group, 20 mg / kg group, respectively. The acute spinal cord injury model was made by modified ^,Allen^,s heavy blow method. The rats in the injury group were injected with normal saline only, and the drug groups were intraperitoneally injected with the different dosages. All the animals were sacrificed at 24 h after the operation. The spinal cord and the vena cava sera of the injured segment were taken for 10 minutes and 2 minutes, respectively. The levels of TNF-αand IL-6 in the spinal cord tissue and serum were detected by enzyme-linked immunosorbent assay（ELISA）. Results: TNF-αand IL-6 in the spinal cord tissue and serum of the rats in the pure injury group were higher than those in the sham group (P <0.05). The levels of TNF-αand IL-6 in spinal cord tissue and serum of rats treated with saikosaponin a 5 mg / kg, 10 mg / kg and 20 mg / kg were lower than those of the injury group, and the difference was statistically significant (P < 0.05). The expressions of TNF-α and IL-6 in the spinal cord tissue of rats treated with 10 mg / group and 20 mg / kg group the difference were not statistically significant (P> 0.05). The levels of TNF-α and IL-6 in serum of rats treated with 10mg / kg and 20 mg/kg were lower than those of 5mg/kg group (P> 0.05). Conclusion: Saikosaponina could reduce the secondary immune inflammatory response by affecting the early expression of inflammatory factors to improve the body’s immune function in acute spinal cord injury. The10mg/kg group is superior to the other groups, and the drug may partially pass through the spinal blood barrier.     

p53和Bcl-2基因蛋白综合表达与喉癌放疗敏感性相关性研究

目的 根据喉癌细胞自身的生物学特性,探讨Bcl-2和雄基因蛋白综合表达与放射治疗敏感性的相互关系。方法 应用抗Bcl-2和p53基因蛋白单克隆抗体,采用DACO CSA System法染色技术,对两组共70例喉鳞癌组织标本进行了免疫组织化学染色分析,将Bcl-2和p53基因表达分3个级别,应用Cramer（修正）列联系数（C）进行检验,探讨各自及综合表达强度与喉癌放疗敏感性间的相关性。结果 可将喉癌Bcl-2和p53基因表达分为强度、中度及阴性表达。基因表达强度与放疗敏感性问具有相关性,综合表达相关性更高。结论 Bcl-2和p53基因蛋白综合表达与放疗敏感性间具有明显相关性,可以把喉癌Bcl-2和p53基因蛋白综合表达作为喉癌放射治疗敏感性预后判定及指导临床治疗的参考指标。     

医学科研的顶层设计——以“恰玛古胶囊干预大肠癌FOLFIRI方案所致腹泻的临床观察”为例

我国部分医学科研存在缺乏有效宏观调控、资源配置不合理、科研力量分散等问题,而将"顶层设计"引入医学科研领域有望改善该问题。以"恰玛古胶囊干预大肠癌FOLFIRI方案所致腹泻的临床观察"为例,浅谈医学科研顶层设计的重要性及必要性,并从其三个环节:科研总体目标和方向、科研组织实施、科研质量控制来阐述顶层设计的实施方法。     

复方骨肽注射液穴位注射治疗颈椎病、腰椎间盘突出、骨关节骨性关节炎等退行性病变和骨质疏松症疼痛临床效果观察

目的：证明复方骨肽注射液穴位注射治疗颈椎病、腰椎间盘突出、骨关节骨性关节炎等退行性病变和骨质疏松症疼痛效果显著方法,复方骨肽注射液穴位注射治疗退行性病变和骨质疏松症疼痛患者77例：结果：复方骨肽注射液穴位注射治疗后止痛效果显著结论：复方骨肽注射液穴位注射治疗操作简单效果显著值得推广应用。