薄膜过滤法在局部给药制剂微生物限度检查中的应用

应用薄膜过滤法检查局部给药制剂的微生物限度。以克霉唑阴道片、聚维酮碘栓、双唑泰栓和硝酸咪康唑乳膏为例，采用薄膜过滤法对细菌数，霉菌及酵母菌数，控制菌进行方法学验证。3次独立的平行试验，试验组的菌回收率均不低于70％；控制菌检查，阳性菌检出，阴性菌未检出；可采用薄膜过滤法测定。对同类局部给药制剂的微生物限度有借鉴作用。     

凝血酶加去甲肾上腺素口服治疗肝硬化上消化道出血24例疗效观察

我院自1995年以来共收治肝硬化合并上消化道出血病人48例，随机分为凝血酶加去甲肾上腺素口服24例（治疗组），垂体后叶素加止血敏静滴24例（对照组），现报道如下。临床资料48例均为住院病人，均经B超、胃镜等检查确诊为肝硬化合并上消化道出血，其中治疗组24例，男13例，女11例，年龄36～7O岁，病史5～21年，出血量8O0～2200ml。对照组24例，男14例，女Ic例，年龄4O～68岁，病史3～19年，出血量600～2000ml。治疗方法除用一般保肝药物，治疗组用凝血酶IOOO”十生理盐水ZOml，去甲肾上腺素3mg十生理盐水ZOml口服，均3次／日；对照组…     

心脏彩超在儿童PSVT中的应用价值

阵发性室上性心动过速（PSVT）是儿童常见的心律失常，是由心房或房室交界处异位兴奋灶快速释放冲动所产生的一种心律失常，病因较多，大多数无器质性心脏病，少部分可由于先天性心脏病、心肌炎、心内膜弹力纤维增生症、     

罗红霉素缓释颗粒微生物限度检验方法的探讨

目的建立罗红霉素缓释颗粒的微生物限度检查方法。方法采用常规法测定霉菌和酵母菌数；用离心薄膜过滤法，即pH7．0氯化钠-蛋白胨缓冲液300mL，每次100mL进行冲洗，测定细菌数；用pH7．0氯化钠-蛋白胨缓冲液500mL，每次100mL进行冲洗，进行控制菌的检查。结果该药对白色念珠菌和黑曲霉菌无抑制作用；离心薄膜过滤法可消除该药物对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌的抑制作用，回收率达80％以上。结论采用离心薄膜过滤法可有效的控制其药物质量，建立的方法准确可靠。     

依达拉奉治疗急性脑梗塞的疗效观察

目的 观察依达托奉治疗急性脑梗塞的疗效.方法 采用随机分组方法将60例诊断明确的脑梗塞患者分为治疗组及对照组,对照组用常规治疗方法,治疗组在此基础上加用依达拉奉30mg静脉滴注,每日2次,2周后进行疗效评定.结果 治疗组总有效率为83.3%,明显优于对照组的66.7%（P〈0.05）,同时治疗组无明显不良反应.结论 依达拉奉治疗急性脑梗塞安全有效,值得临床推广.     

胶质母细胞瘤MGMT基因启动子甲基化与蛋白表达

Objective To study the correlation of MGMT gene promoter methylation and protein expression and their regional variation in different specimens obtained from different regions within the tumor in patients with newly diagnosed glioblastoma. Methods Two to four samples in the same tumor were collected from different regions in 30 patients with newly diagnosed glioblastoma patients. In five patients among them,mutispecimens were obtained under assistance of neuronavigation system during the operation. In all samples,MGMT promoter profile were analyzed by Methylation - specific polymerase - Chain - reaction analysis, MSP ,while MGMT protein expression was detected in tissue sections by immunohistochemistry,IHC. Results MGMT promoter methylation was detected in 43. 56% (44/101) specimens. MGMT protein expression in tissue sections was assessed and scored:(1 :no or positive tumor cells < 10%, 2: ≥ 10% ≤50% ,3: > 50%) ,The rate of MGMT staining with a score 1,2,3 in all of tumor sections was 32. 67% ,43.56% ,23. 76% respectively. No significant correlation between MGMT protein expression and promoter methylation(x2 =2. 905, P =0.088) was found. The regional heterogeneity of MGMT protein expression within the same tumor was in 57% (17/30) patients ;and the regional heterogeneity of gene promoter methylation was in37%(11/30)patients. Conclusions MGMT promoter methylation is probably not the only modulating element in MGMT protein expression. The heterogeneity of MGMT protein expression and its promoter methylation in the same tumor questions their guiding significance in making therapeutic scheme for individual patients with malignant glioma in clinical practice.     

补虚通腑降逆法治疗顽固性呃逆23例

通过对现代及古代医学中有关呃逆证的病因病机的阐述,进一步探讨顽固性呃逆的中医治疗方法,认为采用中医辨证论治的方法,运用指压穴位及补虚、通腑、降逆等疗法治疗顽固性呃逆,可取得较之西医更为满意的疗效。以期对临床治疗顽固性呃逆提供一定的借鉴．     

光量子氧透射液体疗法治疗急性脑梗死36例

<正>2002-01～2002-07笔者所在科收治急性脑梗死患者66例。随机分为光量子氧透射液体疗法组36例(治疗组),男22例,女14例,年龄45～73岁,平均(57.5±6.3)岁;常规组30     

HPLC法测定阿奇霉素片的含量

目的:建立HPLC法测定阿奇霉素片的含量.方法:采用Shodex Asahipak ODP-50(4.6 mm×250mm,5μm)C18柱,流动相为0.04 moL·L-1磷酸氢二钾溶液(称取6.97 g磷酸氢二钾,加水750 mL使溶解,用1moL·L-1的氢氧化钾溶液调节pH值至11.0±0.1再加水稀释至1 000 mE)-乙腈(40:60),流速为1.0 mE·min-1,检测波长为215 mn;柱温40℃;结果:阿奇霉素的线性范围为2.187～8.399 g·L-1,平均回收率为99.9%(RSD 0.15%).结论:本方法简便、迅速、灵敏度高、重现性好,可用于测定阿奇霉素片的含量.