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131.
132.
李东升 《人人健康:医学导刊》2012,(15):64
印度99岁老人辛格·乔吉德尔,不久前在荷兰举行的世界元老运动员田径锦标赛上,以20.8秒的成绩击败所有90岁以上的对手,荣获100米赛冠军。在谈起养生秘诀时,他透露说":不喝酒,不抽烟,不吃肉,每天喝四公升奶,每天锻炼四小时。"曾经被评为中国"长寿之王"的贵州仡佬族老人龚来发今年已131岁,他一生务农,不喝酒,不服药,每天只吃两餐大米和玉米做的混和饭。而已经105岁的女寿星王玉 相似文献
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134.
高血压脑出血( hypertensive intracerebral hemorrhage,HICH)是指继发于高血压( HT)的原发于脑实质的出血性疾病,其发病率高,为(50~80)/10万人[1],发病快,短时间内危及生命。及时有效清除血肿,解除脑组织受压,减轻脑组织损伤是治疗的关键。对于出血30 mL以上或深部脑出血未形成脑疝或中线移位未达到1 cm者,应用传统方法对其行开颅血肿清除、去骨瓣减压手术治疗,一般创伤大,手术时间长,给患者带来一定不良影响,并且需二次手术行颅骨修补,对患者身心及经济负担均带来影响。而微创手术治疗HICH疗效确切,并缩短了手术时间,减少了住院费用,使脑出血病死率不断下降,在临床医学上获得了广泛的应用。我们应用软硬通道穿刺针微创手术治疗HICH 115例,探讨软硬通道在治疗不同部位脑出血中的应用。 相似文献
135.
竹叶总黄酮对缺血再灌注损伤大鼠心功能的影响 总被引:1,自引:1,他引:0
目的:探讨竹叶总黄酮对缺血再灌注损伤大鼠心功能的影响。方法:采用左冠状动脉结扎的方法复制大鼠缺血再灌注损伤模型。分别测定假手术组、模型组、辛伐他汀组和竹叶总黄酮组的左室收缩压(LVSP),左室舒张末压(LVEDP)及左室最大压力上升及下降速率(±dp/dtm ax)并记录心律失常发生的类型和频率。结果:与模型组比较,竹叶总黄酮组、辛伐他汀组LVSP和+dp/dtm ax均显著升高(P<0.01),LVEDP和心律失常的发生均减少(P<0.01,P<0.05);竹叶总黄酮组+dp/dtm ax显著高于辛伐他汀组(P<0.05)。结论:竹叶总黄酮能够保护缺血再灌注损伤大鼠的心功能。 相似文献
136.
BACKGROUND: In vitro cultures of neural stem cells have shown that estrogen can regulate beta-amyloid precursor protein (β-APP) metabolism and reduce amyloid-beta production.
OBJECTIVE: To investigate the effects of long-term oral administration of compound nylestriol or low-dose 17beta-estradiol on β-APP and mRNA expression in the hippocampus of ovariectomized (OVX) rats.
DESIGN, TIME AND SETTING: This randomized and controlled experiment was performed at the Animal Laboratory and Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University between April 2003 and May 2004. MATERIALS: According to body mass, 50 six-month-old female Sprague-Dawley rats were randomly divided into five groups (n = 10 per group): normal control, sham operation, OVX model, 17beta-estradiol (Sigma, USA), and compound nylestriol tablet (Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University) groups.
METHODS: Rats in OVX plus 17beta-estradiol and OVX plus compound nylestriol tablet groups underwent ovariectomy. On the second day after surgery, rats were intragastrically given 17beta-estradiol (100 μg/kg), once per day or compound nylestriol tablet (0.5 mg/kg) and levonorgestrel (0.15 mg/kg) every 2 days. MAIN
OUTCOME MEASURES: β-APP expression in the hippocampus of OVX rats was determined using immunohistochemistry (SABC method) and β-APP mRNA expression was analyzed by in situ hybridization. The results were quantitatively analyzed using cell counting and average optical density.
RESULTS: The number and optical density of β-APP-positive neurons in every subregion of the hippocampus of OVX rats was dramatically increased compared with normal and sham operation groups following 35 weeks of administration (P 〈 0.05). Levels of β-APP were decreased following oral administration of compound nylestriol or 17beta-estradiol. In situ hybridization showed that long-term estrogen deficiency and oral administration of compound nylestriol or 17beta-estradiol did not alter the number of β-APP mRNA-positive neurons.
CONCLUSION: The results show that long-term estrogen deficiency results in an increase of expression of β-APP though no changes in the expression of β-APP mRNA are detected. Replacement of estrogen with low-dose 17 beta-estradiol or compound nylestriol tablet inhibits the expression of β-APP in the hippocampus to the same extent. 相似文献
OBJECTIVE: To investigate the effects of long-term oral administration of compound nylestriol or low-dose 17beta-estradiol on β-APP and mRNA expression in the hippocampus of ovariectomized (OVX) rats.
DESIGN, TIME AND SETTING: This randomized and controlled experiment was performed at the Animal Laboratory and Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University between April 2003 and May 2004. MATERIALS: According to body mass, 50 six-month-old female Sprague-Dawley rats were randomly divided into five groups (n = 10 per group): normal control, sham operation, OVX model, 17beta-estradiol (Sigma, USA), and compound nylestriol tablet (Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University) groups.
METHODS: Rats in OVX plus 17beta-estradiol and OVX plus compound nylestriol tablet groups underwent ovariectomy. On the second day after surgery, rats were intragastrically given 17beta-estradiol (100 μg/kg), once per day or compound nylestriol tablet (0.5 mg/kg) and levonorgestrel (0.15 mg/kg) every 2 days. MAIN
OUTCOME MEASURES: β-APP expression in the hippocampus of OVX rats was determined using immunohistochemistry (SABC method) and β-APP mRNA expression was analyzed by in situ hybridization. The results were quantitatively analyzed using cell counting and average optical density.
RESULTS: The number and optical density of β-APP-positive neurons in every subregion of the hippocampus of OVX rats was dramatically increased compared with normal and sham operation groups following 35 weeks of administration (P 〈 0.05). Levels of β-APP were decreased following oral administration of compound nylestriol or 17beta-estradiol. In situ hybridization showed that long-term estrogen deficiency and oral administration of compound nylestriol or 17beta-estradiol did not alter the number of β-APP mRNA-positive neurons.
CONCLUSION: The results show that long-term estrogen deficiency results in an increase of expression of β-APP though no changes in the expression of β-APP mRNA are detected. Replacement of estrogen with low-dose 17 beta-estradiol or compound nylestriol tablet inhibits the expression of β-APP in the hippocampus to the same extent. 相似文献
137.
目的 观察脐血间充质干细胞移植治疗糖尿病足的疗效和安全性.方法 将脐血间充质干细胞多点注射到自愿接受干细胞移植的2例糖尿病足患者的病变下肢,细胞数(3~7)×107/L,每点0.3~0.5 ml,每点间距3 cm×3 cm,肌肉组织丰富的部位可分层注射.移植术后第1天~3个月定期观察患者的临床症状及各项指标的变化并进行综合分析.结果 脐血间充质干细胞移植后3个月进行评价,2例患者下肢疼痛均明显缓解、皮温升高、皮肤凉感消失、间歇性跛行明显改善、足部溃疡愈合.踝肱指数、经皮氧分压较治疗前有明显升高,CT血管造影显示治疗后病变下肢均有丰富的侧支血管生成.移植后2例患者均未出现严重并发症和明显不良反应,无一例患者行截肢术.结论 脐血间充质干细胞移植治疗糖尿病下肢缺血性血管病是一种安全、有效的手段,可使部分患者免除截肢. 相似文献
138.
目的 探讨pyk2小干扰RNA表达载体对小鼠脑胶质瘤G422细胞增殖、侵袭能力的影响.方法 用以pGenesil-1质粒为载体,以pyk2为靶基因,构建短发夹状小干扰RNA表达载体.用pyk2短发夹状小干扰RNA表达载体转染体外培养的人脑胶质瘤G422细胞,反转录聚合酶链反应和Western blot法检测pyk2基因和蛋白表达的改变,噻唑蓝(MTT)比色法检测转染后细胞的增殖情况,Transwell小室法测定转染细胞体外侵袭能力.结果 构建了pGenesil-1-pyk2重组质粒,并成功转染G422细胞.逆转录-聚合酶链反应(RT-PCR)显示pyk2 siRNA转染G422细胞后显著降低pyk2mRNA的表达,以48 h为著,下降近70%;Western blot证实转染后pyk2蛋白的表达下降近65%;G422细胞的活力降低为(67.1±5.2)%;Transwell小室测定转染细胞体外侵袭能力明显下降.结论 pGenesil-1-pyk2 siRNA重组质粒明显下调pyk2在胶质瘤细胞中的表达,并抑制肿瘤细胞增殖,抑制其侵袭能力. 相似文献
139.
HPLC法测定生脉饮中五味子醇甲的含量 总被引:1,自引:0,他引:1
目的:建立一种生脉饮中五味子醇甲的高效液相色谱测定方法.方法:Hypersilbds(250mm×4.6mm,5μm)为色谱柱:用甲醇-水(62:38)为流动相;以外标法按峰面积计算.结果:五味子醇甲在8.4-84μml范围呈良好的线性关系,该法回收率为99.62%.结论:该法简便快捷,结果准确,可用于生脉饮中五味子醇甲的含量测定和质量控制. 相似文献
140.
心房颤动(房颤)为临床上常见的心律失常之一,据Framinhan的研究报告提示,其人群发病率在1%左右,且随着年龄增长其发病率增加。房颤能够引发心力衰竭、血栓栓塞、中风等危险,严重威胁人类健康。1997年以来,消融肺静脉治疗房颤成为一种新的可行的治疗手段。我科于2005年11月20日对2例阵发性心房颤动(paroxysmal atrial fibrillation,PAF)患者成功进行了单Lasso指导下肺静脉电隔离术,取得了很好的效果。报道如下. 相似文献