The effect of vascular endothelial growth factor on the healing of ischaemic skin wounds.

The effect of exogenous vascular endothelium growth factor (VEGF) on wound healing in an ischaemic skin flap model was evaluated in this study. Seventy-two Sprague-Dawley rats were used. Normal incisional wound and H-shaped double flaps were used as the wound models. The study was divided into two parts. In Part I, VEGF protein levels were determined from the incisional and H-shaped ischaemic wounds at 12 and 24 h, postoperatively. In Part II, tensile strength and immunohistochemical stains were examined to determine the level of microvessel density (MVD) at 1 and 2 weeks, postoperatively in simple incisional wounds, ischaemic wounds, and ischaemic wounds following 1 ml (1 microg/ml) exogenous VEGF injections into the subcutaneous tissue. The results showed a significantly higher level of VEGF protein in the ischaemic wounds than the incisional wounds. Tensile strength was statistically higher in the incisional wound group and in the ischaemic flap wounds with VEGF treatment compared to the ischaemic flaps with no treatment at 1 week, postoperatively (p>0.05). MVD data indicated that ischaemic wound repair with VEGF treatment had significantly higher MVD than the normal incisional wounds and ischaemic wounds without treatment. We conclude that exogenous application of VEGF can increase early angiogenesis and tensile strength in the ischaemic wound.     

抗人生长激素免疫血清的制备和鉴定

Using small dosage of human growth hormone to immunize rabbit or guinea pig, it is able to induce anti-hGH antibody formation with high titre and high affinity that could be applied to hGH RIA. In the present study five rabbits and three guinea pigs were immunized with 125-200 micrograms and 250-285 micrograms per animal of hGH respectively, followed by boosters of 10-20 or 160-250 micrograms of hGH at 2-4 week intervals for 6 or 3 months. Blood was drown 1-2 weeks before each booster for determination of antibody formation. Antibody titre and affinity were successively observed and specificity of antibody was determined for the final bleeding. It was shown that titres of immune sera from guinea pigs were much higher than those of rabbit immune sera, but vice versa for antibody affinity. This might be due to larger immunogen dose used for guinea pigs than for rabbits. Fourteen different peptide hormones were tested in reference to cross-immunoreactivity to anti-hGH antibody. It could be demonstrated that the major cross-reactive hormones are hFSH and hLH, and hTSH also reacts to rabbit anti-hGH immune sera at a lesser degree. These cross reactivities are obviously owing to the molecular homogeneities between hGH and these hormones especially of their alpha-subunits.     

紫外分光光度法测定对乙酰氨基酚泡腾片含量

本文用紫外分光光度法在２５７±１ｎｍ波长处测定对乙酰氨基酚泡腾片的含量，其浓度与吸收度的线性关系良好，相关系数ｒ＝０．９９９９８。平均回收率为９９．９４％，变异系数为０．２０％。本法简便、快速、结果准确。     

4-[(4-氯苯基)(5-氟-2-羟基苯基)亚甲氨基]丁酸酯类的合成及其抗惊厥活性

作者设计并合成了8个γ-氨基丁酸(GABA)的衍生物(Ⅳ_(a-b)),使GABA的酯通过亚胺键,连接在一个易于通过血脑屏障的亲脂性载体上。合成的8个标题化合物,均用NIH提出的抗癫痫药物开发程序进行评价,结果化合物Ⅳ_b和Ⅳ_f表现出有效的抗惊厥活性。     

Problems in the design of anticancer agents

Theoretically, two predominant paths for obtaining more selective anticancer agents may be envisaged. These are: (a) to make compounds which distribute only or preferentially in cancer cells; (b) to make compounds that are able selectively to kill or to differentiate cancer cells. Although in the last two decades research into new anticancer drugs has not produced satisfactory results, there is solid ground on which novel strategies can be developed, mainly based on a much greater biological knowledge of human tumours. This article does not review all the possible approaches that may be followed, but simply discusses some ideas and problems mainly taken from the current research of our laboratory.     

台湾产奇特长唇瓢虫雄性外生殖器与两新种描述：...

本文描述了奇特长唇瓢虫Shiro(?)lla mirol(?)ilis Sasaji的雄性外生殖器与本属两新种:附肢长唇瓢虫Sh.oppe(?)nd(?)a sp.n.,阿里山长唇瓢虫Sh.(?) sp.n.,均产于台湾.种类检索如下:1(2).后基线伸达第一腹板1/2略强:阳基侧叶长,无触须状附肢;鞘翅淡黄棕色,具七个黑色斑纹;体 长1.82 mm………………………………………………………阿里山长唇瓢虫Sh.(?)sp.n.2(1).后基线后缘远超第一腹板长度的1/2;阳基侧叶短,具触须状附肢:3(4).前胸背板棕色,具两个暗棕色模糊斑点;鞘翅棕色,具九个黑斑;体长2.12～2.59 m………… …………………………………………………………………………附肢长唇瓢虫Sh.(?)sp.n.4(3).前胸背板淡黄棕色,无斑点;鞘翅淡黄棕色,具六个黑斑,侧面的一对长形;体长2.00～2.26mm …………………………………………………………………………奇特长唇瓢虫Sh.(?)sasaji     

Vascular pharmacology of methylene blue in vitro and in vivo: a comparison with NG-nitro-L-arginine and diphenyleneiodonium.

1. The vascular effects of the soluble guanylyl cyclase inhibitor, methylene blue as well as the nitric oxide (NO) synthase inhibitors, NG-nitro-L-arginine (L-NOARG) and diphenyleneiodonium (DPI) were studied in rat isolated aortic rings and conscious, unrestrained rats. 2. Acetylcholine (ACh) and sodium nitroprusside (SNP) caused concentration-dependent relaxation of preconstricted aortic rings. Both methylene blue (1 x 10(-5) M) and L-NOARG (3 x 10(-5) M) abolished ACh-induced relaxation; however, methylene blue but not L-NOARG shifted the concentration-response curve of SNP to the right. 3. In conscious rats, i.v. infusion of methylene blue (1.1 x 10(-5) mol kg-1 min-1), at a concentration which reduced the aortic tissue level of cyclic GMP by 50%, did not significantly alter mean arterial pressure (MAP) and heart rate (HR). In contrast, i.v. bolus injection of L-NOARG (1.5 x 10(-4) mol kg-1) markedly increased MAP and decreased HR. 4. Both ACh and SNP dose-dependently decreased MAP in conscious rats. Methylene blue did not alter the magnitude or duration of ACh- or SNP-induced depressor responses. L-NOARG, on the other hand, significantly though incompletely, reduced the magnitude and duration of the depressor response to ACh but not SNP. The depressor response to ACh or SNP was not altered by pretreatment with indomethacin (1.4 x 10(-5) mol kg-1) or capsaicin (3.3 x 10(-4) mol kg-1). 5. NG-nitro-L-arginine methyl ester (L-NAME) also caused dose-dependent increases in MAP in conscious rats.(ABSTRACT TRUNCATED AT 250 WORDS)     

腋淋巴结阴性乳腺癌TGFβl和VEGF表达与预后的相关性研究

目的：探讨转化生长因子(TGF)βl和血管内皮生长因子(VEGF)与腋淋巴结阴性乳腺癌(ANNBC)临床病理及强后的关系。方法：应用免疫组化技术(LDP法)，对56例随访资料完整的女性ANNBC根治标本进行TGFβl和VEGF检测，分析其表达与ANNBC的预后关系。结果：TGFβl和VEGF、表达与ANNBC的年龄、肿瘤大小、病理类型、组织学分级等一般临床病理因素无相关(P＞0．05)，而与肿瘤复发、转移密切相关，TGFβl和VEGF阳性表达的肿瘤复发转移率明显高于阴性表达者(P＜0．01)；TGFβl和VEGF表达呈正相关(P＜0．05)；Cox比例风险模型多因素分析表明，TGFβl和VEGF是影响ANNBC的预后因子，TGFβl和VEGF表达的ANNBC病人预后差。结论：①测定TGFβl和VEGF的表达，对判断ANNBC病人预后及指导治疗有较大的帮助；②TGFβl和VEGF阳性表达与ANNBC血管生成密切相关，是ANNBC复发、转移的重要促进因素。