人血栓调节蛋白基因真核表达质粒的构建

目的：构建人血栓调节蛋白(human thrombomodulin，hTM)基因的真核表达质粒，为研究hTM在抗血栓、抗炎症、抗血管增生等方面的作用及其临床应用奠定基础。方法：用PCR法扩增出hTMcDNA表达片段。把载体质粒pcDNA3．1( )／neo和hTM片段用EcoRⅠ HindⅢ双酶切，T4DNA连接酶进行连接。结果：重组质粒在大肠杆菌菌株DH5α内扩增。提取、纯化后用HindⅢ、EcoRⅠ酶切鉴定及测序鉴定证明hTMcDNA表达片段已被完整、正确的插入到pcDNA3．1( )／neo质粒中。结论：成功构建了hTM基因的真核表达质粒pcDNA-hTM。     

激光扫描共聚焦显微镜在缺血性心脏病研究中的应用

激光扫描共聚焦显微镜 (ｌａｓｅｒｓｃａｎｎｉｎｇｃｏｎｆｏｃａｌｍｉｃｒｏｓｃｏｐｅ ,ＬＳＣＭ)是紫外和可见激光双重扫描相结合的显微镜 ,由于用点光源代替场光源 ,使探测点与照明点共扼 ,有效地抑制了同一焦平面上的杂散荧光以及来自非焦平面的荧光 ,使横向分辨率比普通光学显微镜和荧光显微镜明显升高。它同时具有深度识别能力 (最大深度为 2 0 0 - 40 0 μｍ)及纵向分辨率 ,因而可以看到较厚的生物标本中的深层的荧光分布细节 ,可以逐层获得高反差、高分辨率、高灵敏度、无损伤的二维光学切片 ,从而具有细胞ＣＴ的功能。ＬＳＣＭ…     

盐酸沙格雷酯早期干预治疗周围动脉疾病的疗效评价

目的观察盐酸沙格雷酯(安步乐克)在肢体慢性缺血性疾病早期应用的临床疗效。方法肢体慢性缺血性疾病163例(共219条肢体)患者,口服安步乐克300mg/日(分3次),共3个月。比较治疗前后患者临床症状和踝肱指数的变化,以了解安步乐克的治疗效果。结果患者服药前后的跛行距离[(200～300)m vs (500～800)m]、平均透皮组织氧分压(62.5mmHg vs 65.4mmHg)及踝肱指数(0.7～0.9 vs 0.8～0.9)均有不同程度的提高,治疗前后比较差异有明显统计学意义(P<0.01)。结论安步乐克在周围动脉疾病的早期治疗中,效果显著,可明显减轻症状。     

胆道镜联合钬激光经T管窦道治疗肝内外胆管残余结石

目的:探讨胆道镜联合钬激光经T管窦道碎石治疗肝内、外胆管残余结石的临床价值和安全性。方法:回顾性分析2010年2月—2011年6月经术后T管窦道置入胆道镜联合钬激光碎石治疗300例肝内、外胆管残余结石患者的临床资料。结果:292例经胆道镜下钬激光碎石治疗结石全部取净,碎石时间4~12 min,碎石次数1~5次,13例经5次碎石;8例患者结石位于III,IV级肝管碎石未成功,带管2个月后经等离子体冲击波碎石,6例患者成功取出结石,2例患者结石未取出。碎石总数568枚,结石排净率97.3%(292/300)。术中无胆道大出血、穿孔、胆管壁灼伤、胆瘘等严重并发症发生;15例术后出现低热。268例随访6~18个月,平均10个月,未发现结石复发及残留,无胆道狭窄。结论:胆道镜联合钬激光碎石可显著提高肝内、外胆管残余结石的治疗效果,是一种简便、安全、有效的方法,值得临床应用推广。     

由OPTN突变引起的成人型原发性开角型青光眼

原发性开角型青光眼 (ＰＯＡＧ)是主要的致盲眼病 ,全世界约有 3千多万患者。目前已知有 8个基因位点与青光眼有关 ,其中 2个基因已被确定 :ＣＹＰ1Ｂ1基因编码细胞色素Ｐ450 1Ｂ1酶 ,与原发性先天性青光眼有关 ;ＭＹＯＣ/ＴＩＧＲ基因编码ｍｙｏｃｉｌｉｎ ,与青少年型ＰＯＡＧ有关。作者报道一个与成人型ＰＯＡＧ有关的基因 ,已被人类基因组织命名委员会确定 ,称为ＯＰＴＮ ,其编码的蛋白产物为ｏｐｔｉｎｅｕｒｉｎ ,含义是引起视神经病变的蛋白。作者所在的实验组于 1 998年通过对一正常眼压性青光眼家系的基因连锁分析 ,发现了定位于 1…     

岳阳市1999年常规免疫接种率监测报告系统评价

目的　了解岳阳市 1999年常规免疫接种率监测系统工作情况。　方法　根据《全国常规免疫接种率监测方案》提供的方法对该市 1999年基础免疫接种率报告数据进行分析和评价。　结果　县级报告率 95 % ,及时报告率89.5 % ,乡级报告率 89.4% ;口服脊髓灰质炎疫苗、卡介苗、百白破混合制剂、麻疹疫苗报告接种率均在 86 .2 %～ 99.8%之间 ,乙肝疫苗报告接种率在 18.7%～ 99.1%之间 ;全市估计接种率 BCG75 .2 %、OPV6 9.6 %、DPT6 9.3%、MV77.9%、HBV2 5 .3% ;一些县报告接种率可信性较低。　结论　加强生物制品、流动儿童免疫和监测系统的管理至关重要 ,提高乡以下报告率和报告质量是完善接种率监测系统的关键。     

螺旋CT和彩色多普勒血流显像检查对胰腺癌可切除性的评估价值

Objective To determine the value of helical computed tomography (HCT) and color doppler flowing imaging (CDPI) in evaluating the resectability of pancreatic cancer. Methods The clinical data of 114 patients with pancreatic cancer who had been admitted to the Affiliated Hospital of North Sichuan Medical College from January 1995 to December 2002 were retrospectively analyzed. The values of HCT and CDPI in assessing the resectability of pancreatic cancer were determined according to the results of operation and pathological examina-tion. All the data were analyzed by chi-square test and Fisher exact probability. Results Of all patients, 109 were examined by HCT, 97 by CDPI and 96 by HCT+CDPI. For patients examined by HCT, the resection rates of pancreatic head cancer, pancreatic body and tail cancer and total pancreatic cancer were 45.3% (39/86), 26.3% (5/19) and 0 (0/9), respectively. The resection rate of pancreatic head cancer was higher than that of pancreatic body and tail cancer (χ2=8.965, P<0.05). With the increase of tumor size, the invasion rate and metastasis rate were increased and the resection rate was decreased (z=6.15, 5.35, 7.18, P<0.01). The sensitivity rate and specificity rate were 77.8% and 82.2% of HCT, 73.3% and 80.6% of CDPI, 90.6% and 92.4% of HCT+CDPI in assessing the resectability of pancreatic cancer. The values of Kappa identity test of HCT, CDPI and HCT+CDPI were 0.58, 0.52 and 0.82, respectively. Conclusions Combined application of HCT and CDPI can further improve the accuracy in assessing the resectability of pancreatic cancer.     

四川女排防守准备姿势的初步研究(文摘)

我国女子排球的后排防守移动能力在近年重大国际比赛中暴露出与日本、美国、南朝鲜等强队的差距很大。有认为我国女排防守失误率高的主要原因是反应移动速度慢,并提出防守移动能力与速度性素质关系密切。本文利用高速摄影技术来研究运动员的防守准备姿势与防守移动速度的关系,为提高防守能力找出较为合理的防守准备姿势。     

螺旋CT和彩色多普勒血流显像检查对胰腺癌可切除性的评估价值

Objective To determine the value of helical computed tomography (HCT) and color doppler flowing imaging (CDPI) in evaluating the resectability of pancreatic cancer. Methods The clinical data of 114 patients with pancreatic cancer who had been admitted to the Affiliated Hospital of North Sichuan Medical College from January 1995 to December 2002 were retrospectively analyzed. The values of HCT and CDPI in assessing the resectability of pancreatic cancer were determined according to the results of operation and pathological examina-tion. All the data were analyzed by chi-square test and Fisher exact probability. Results Of all patients, 109 were examined by HCT, 97 by CDPI and 96 by HCT+CDPI. For patients examined by HCT, the resection rates of pancreatic head cancer, pancreatic body and tail cancer and total pancreatic cancer were 45.3% (39/86), 26.3% (5/19) and 0 (0/9), respectively. The resection rate of pancreatic head cancer was higher than that of pancreatic body and tail cancer (χ2=8.965, P<0.05). With the increase of tumor size, the invasion rate and metastasis rate were increased and the resection rate was decreased (z=6.15, 5.35, 7.18, P<0.01). The sensitivity rate and specificity rate were 77.8% and 82.2% of HCT, 73.3% and 80.6% of CDPI, 90.6% and 92.4% of HCT+CDPI in assessing the resectability of pancreatic cancer. The values of Kappa identity test of HCT, CDPI and HCT+CDPI were 0.58, 0.52 and 0.82, respectively. Conclusions Combined application of HCT and CDPI can further improve the accuracy in assessing the resectability of pancreatic cancer.     

合理利用能量器械,提高手术效果和安全性

各种能量器械的应用推动了妇科微创手术的发展和普及。目前临床常用的能量器械包括:电能量、超声和激光等,不同的能量器械工作原理是不同的。妇科临床医生应该要熟练掌握不同能量器械的工作原理和特点。规范使用、合理使用能量器械始终是保证手术安全的惟一法宝。