变应性鼻炎血清学特异性IgE检测的结果分析

目的:调查本地区变应性鼻炎患者变应原血清特异性IgE阳性率,比较不同年龄和性别患者血清特异性IgE阳性率的差别。方法:对145例变应性鼻炎患者进行血清吸入性和食入性变应原特异性IgE检测,并将受检者按年龄及性别分组,分别进行阳性率比较。结果:145例变应性鼻炎患者变应原特异性IgE总阳性94例,阳性率64.8%。其中,单一变应原阳性45例(47.9%),两种及以上变应原阳性49例(52.1%);以吸入性变应原为主。男性与女性变应原特异性IgE阳性率比较差异无统计学意义(P>0.05);成年人与青少年阳性率比较差异有统计学意义(P<0.05)。结论:本地区变应性鼻炎患者中吸入性变应原是引起发病的主要原因,其中户尘螨粉尘螨阳性率最高,占87.4%。特异性IgE阳性率与年龄有关,青少年较成年人阳性率高。     

紫癜风(过敏性紫癜)诊治方案应用分析

目的 通过对国家中医药管理局重点专科血液病协作组紫癜风(过敏性紫癜)协作分组各单位提供的紫癜风诊疗方案进行梳理,得出各单位的共识.方法 采用定性分析方法,对紫癜风的临床疗效、中医治疗方法、治疗难点等进行系统分析.结果与结论 对中医治疗紫癜风的难点提出了应对思路.分析整理后的经过多名专家认可的总结诊疗方案可以在全国推广使用.     

O/W型丹皮酚微乳处方设计及质量评价

目的研究O/W型丹皮酚微乳的处方工艺,并建立其含有量的测定方法。方法选用丁香油为油相,Tween80、Tween20、Poloxamer188、OP-10、SolutolHS 15为表面活性剂,PEG400、甘油、1,2-丙二醇、乙醇、异丙醇、正丁醇为助表面活性剂,通过绘制伪三元相图,探讨各因素对微乳形成的影响,优选微乳处方,并初步考察丹皮酚微乳理化性质,用HPLC法测定微乳中丹皮酚的含有量。结果丹皮酚微乳稳定,平均粒径为63.4 nm,20℃恒温下丹皮酚微乳的黏度为23.38 mPa.s,电导率为31μs/cm,折光率为1.444 9。HPLC测定线性关系良好,方法平均回收率为99.22%,RSD为1.43%。结论 O/W型丹皮酚微乳制剂稳定,测定方法可靠,重复性好。     

人纤维介素蛋白2在昆虫杆状病毒系统中的表达及活性检测

目的:利用杆状病毒表达系统在昆虫细胞(Sf9细胞)中表达人纤维介素蛋白2(hfgl2)凝血酶原酶并检测其蛋白活性.方法:首先将PCR扩增的目的基因hfgl2和pENTR/D-TOPO载体连接,再将pENTR/D-TOPO-hfgl2质粒与BaculoDirectTM线性DNA进行体外基因重组,采用脂质体转染法将重组DNA转染到生长良好的Sf9细胞中进行病毒包装,经过对重组杆状病毒三轮扩增后,用RT-PCR和Western blotting从基因和蛋白水平检测hfgl2表达情况,一期凝固试验检测其蛋白活性.结果:重组杆状病毒感染后,Sf9细胞停止生长,体积变大,细胞开始悬浮,破裂,呈现颗粒样外观.RT-PCR扩增和Western blotting从基因和蛋白水平证实Sf9细胞能够表达hfgl2,蛋白分子量约为63kDa.同时,在细胞上清液中检测到了可溶性hfgl2的表达,蛋白分子量约为50kDa.一期凝固试验证明表达的跨膜型hfgl2具有凝血活性.结论:hfgl2在昆虫杆状病毒表达系统中成功表达并具有生物学活性,为进一步研究hfgl2跨膜型以及可溶性蛋白功能提供了工具,亦可应用于开发重型肝炎蛋白芯片或ELISA诊断试剂盒.     

Nipple Adenoma in an Adolescent

Abstract: Nipple adenoma is a rare tumor of the breast which usually appears in middle age women and rarely in adolescents. We describe a 15‐year‐old female diagnosed with nipple adenoma. Nipple adenoma is a benign neoplasia which should be recognized to avoid confusion with breast cancer.     

配合物显色光度法测定土壤中微量铅

研究了双硫腙分光光度法测定土壤中铅的方法。采用双硫腙为显色剂,控制溶液的p H为8.5,在513nm波长处测定吸光度,利用标准曲线求出铅含量。讨论了溶液p H、反应时间、络合剂用量对吸光度的影响。     

脑静脉窦血栓形成的磁共振诊断价值分析

脑静脉窦血栓形成远比脑动脉血栓少见，由于其临床表现缺乏特异性，常导致误诊。磁共振成像（MRI）和磁共振血管成像（MRA）的临床应用，为脑静脉窦血栓形成的诊断提供了新的非创伤性影像检查手段。本文回顾性分析我院自2006年8月～2007年11月12例脑静脉窦血栓形成患者的MRI表现，旨在分析探讨MRI对脑静脉窦血栓形成的影像诊断价值。     

Technetium-99m DTPA renography and angiography in renal artery stenoses of varying severity

Thirty-five hypertensive patients, consecutively studied between 1986 and 1991, were retrospectively reviewed, to compare Technetium-99m (^99mTc) DTPA renal scanning for renal artery stenosis with angiography. Ten patients were on chronic angiotensin converting enzyme (ACE) inhibitor medication and 20 underwent angioplasty of their stenoses. In patients not on ACE inhibitors the renal scan specificity was 83%, sensitivity 82%, negative predictive value 89% and positive predictive value 74%. In the 10 patients on ACE inhibitors the specificity was 67% (relatively more stenoses of < 50% were reported in this group), sensitivity 100%, negative predictive value 100% and positive predictive value 60%. Low levels of function (<10mL/min/1.73m²) in 9 kidneys did not militate against diagnoses that correlated well with angiography. Hence, renal scanning, particularly with ACE inhibition, is efficacious in the evaluation of possible renal artery stenosis. With a mean follow-up period of 29 months after percutaneous transluminal angioplasty (PTA), four patients had clinical improvement of hypertension control. Each had pre-PTA total glomerular filtration rates (GFR) of approximately 75mL/min/1.73m². No improvement was detected in cases with pre-PTA total GFR of <50mL/min/1.73m². This may be the level of renal function below which PTA therapy or surgery will not provide benefit.