全文获取类型
收费全文 | 12703篇 |
免费 | 962篇 |
国内免费 | 38篇 |
专业分类
耳鼻咽喉 | 128篇 |
儿科学 | 471篇 |
妇产科学 | 216篇 |
基础医学 | 1673篇 |
口腔科学 | 176篇 |
临床医学 | 1285篇 |
内科学 | 2568篇 |
皮肤病学 | 117篇 |
神经病学 | 1135篇 |
特种医学 | 484篇 |
外国民族医学 | 1篇 |
外科学 | 1787篇 |
综合类 | 283篇 |
一般理论 | 9篇 |
预防医学 | 1142篇 |
眼科学 | 305篇 |
药学 | 961篇 |
中国医学 | 45篇 |
肿瘤学 | 917篇 |
出版年
2023年 | 53篇 |
2022年 | 64篇 |
2021年 | 244篇 |
2020年 | 154篇 |
2019年 | 268篇 |
2018年 | 282篇 |
2017年 | 202篇 |
2016年 | 224篇 |
2015年 | 251篇 |
2014年 | 424篇 |
2013年 | 561篇 |
2012年 | 868篇 |
2011年 | 902篇 |
2010年 | 473篇 |
2009年 | 467篇 |
2008年 | 774篇 |
2007年 | 917篇 |
2006年 | 874篇 |
2005年 | 850篇 |
2004年 | 751篇 |
2003年 | 709篇 |
2002年 | 650篇 |
2001年 | 160篇 |
2000年 | 127篇 |
1999年 | 136篇 |
1998年 | 144篇 |
1997年 | 139篇 |
1996年 | 115篇 |
1995年 | 110篇 |
1994年 | 95篇 |
1993年 | 99篇 |
1992年 | 105篇 |
1991年 | 65篇 |
1990年 | 62篇 |
1989年 | 80篇 |
1988年 | 78篇 |
1987年 | 84篇 |
1986年 | 69篇 |
1985年 | 81篇 |
1984年 | 91篇 |
1983年 | 61篇 |
1982年 | 68篇 |
1981年 | 68篇 |
1980年 | 66篇 |
1979年 | 44篇 |
1978年 | 41篇 |
1977年 | 36篇 |
1974年 | 35篇 |
1973年 | 39篇 |
1972年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
Müllerian duct regression is first apparent in male pouch young of the tammar wallaby (Macropus eugenii) 6–7 days after birth and, as in eutherian mammals, is characterised by a condensation of the periductal mesenchyme into
a whorl around the ductal epithelial cells. A decrease in the density of the extracellular matrix was observed in the region
of the whorl. In contrast to eutherian mammals no changes were observed in the mean outer diameter of the Müllerian duct during
the early stages of regression. The time at which these mesenchymal changes occur corresponds to the period of Müllerian inhibiting
substance secretion in the postnatal tammar testis.
Accepted: 25 February 1997 相似文献
22.
Brigden Geoffrey Zanelli Giussepe Lahiri Avijit Raftery Edward 《European journal of nuclear medicine and molecular imaging》1990,16(11):795-799
A new, single bolus method of in vivo blood pool imaging using a technetium Tc99m phosphine isocyanide complex (DEPIC) which binds to pre-albumin was evaluated in volunteers (n=4) and patients (n=20). DEPIC was assessed for its safety and possible drug interactions. Its duration of action and quality of ventriculography were compared with imaging using standard in vivo red cell labelling (PYP) during two 3-h scanning periods 1 week apart. DEPIC had a mean plasma halflife of 3.3 h. The count rate over the left ventricle was initially 42% higher with DEPIC than with PYP. However, removal of DEPIC by the liver resulted in equivalent count rates by 1 h, and by 3 h PYP count rates were 22% higher than DEPIC. Immediately post injection mean (SD) difference in the left ventricular ejection fraction between the two methods was 2.4% (7.7%). Satisfactory DEPIC scans were obtained up to 2 h post injection, but by 3 h there was a mean difference of 13% (11.3%). DEPIC was found to be a safe alternative to red all labelling for blood pool angiography, suitable for routine work. The single bolus methodology and high initial count rates offer improved efficiency and a capability for truly emergency scanning. 相似文献
23.
Serum and Cerebrospinal Fluid Pharmacokinetics of Intravenous and Oral Lamivudine in Human Immunodeficiency Virus-Infected Children 总被引:3,自引:3,他引:0 下载免费PDF全文
Brigitta U. Mueller Linda L. Lewis Geoffrey J. Yuen Maureen Farley Amy Keller Joseph A. Church Jonathan C. Goldsmith David J. Venzon Marc Rubin Philip A. Pizzo Frank M. Balis 《Antimicrobial agents and chemotherapy》1998,42(12):3187-3192
We studied the pharmacokinetics of intravenously and orally administered lamivudine at six dose levels ranging from 0.5 to 10 mg/kg of body weight in 52 children with human immunodeficiency virus infection. A two-compartment model with first-order elimination from the central compartment was simultaneously fitted to the serum drug concentration-time data obtained after intravenous and oral administration. The maximal concentration at the end of the 1-h intravenous infusion and the area under the concentration-time curve after oral and intravenous administration increased proportionally with the dose. The mean clearance of lamivudine (± standard deviation) in the children was 0.53 ± 0.19 liter/kg/h (229 ± 77 ml/min/m2 of body surface area), and the mean half-lives at the distribution and elimination phases were 0.23 ± 0.18 and 2.2 ± 2.1 h, respectively. Clearance was age dependent when normalized to body weight but age independent when normalized to body surface area. Lamivudine was rapidly absorbed after oral administration, and 66% ± 25% of the oral dose was absorbed. Serum lamivudine concentrations were maintained above 1 μM for ≥8 h of 24 h on the twice daily oral dosing schedule with doses of ≥2 mg/kg. The cerebrospinal fluid drug concentration measured 2 to 4 h after the dose was 12% (range, 0 to 46%) of the simultaneously measured serum drug concentration. A limited-sampling strategy was developed to estimate the area under the concentration-time curve for concentrations in serum at 2 and 6 h. 相似文献
24.
25.
Werner E Bischoff Brian K Tucker Michelle L Wallis Beth A Reboussin Michael A Pfaller Frederick G Hayden Robert J Sherertz 《Infection control and hospital epidemiology》2007,28(10):1148-1154
OBJECTIVE: Transmission of Staphylococcus aureus via air may play an important role in healthcare settings. This study investigates the impact of barrier precautions on the spread of airborne S. aureus by volunteers with experimentally induced rhinovirus infection (ie, the common cold). DESIGN: Prospective nonrandomized study. SETTING: Wake Forest University School of Medicine (Winston-Salem, NC).Participants. A convenience sample of 10 individuals with nasal S. aureus carriage selected from 593 students screened for carriage. INTERVENTION: Airborne S. aureus dispersal was studied in the 10 participants under the following clothing conditions: street clothes, surgical scrubs, surgical scrubs and a gown, and the latter plus a face mask. After a 4-day baseline period, volunteers were exposed to a rhinovirus, and their clinical course was followed for 12 days. Daily swabs of nasal specimens, pharynx specimens, and skin specimens were obtained for quantitative culture, and cold symptoms were documented. Data were analyzed by random-effects negative binomial models. RESULTS: All participants developed a common cold. Incidence rate ratios (IRRs) indicated that, compared with airborne levels of S. aureus during sessions in which street clothes were worn, airborne levels decreased by 75% when surgical scrubs were worn (P<.001), by 80% when scrubs and a surgical gown were worn (P<.001), and by 82% when scrubs, a gown, and a face mask were worn (P<.001). The addition of a mask to the surgical scrubs and gown did not reduce the airborne dispersal significantly (IRR, 0.92; P>.05). Male volunteers shed twice as much S. aureus as females (incidence rate ratio, 2.04; P=.013). The cold did not alter the efficacy of the barrier precautions. CONCLUSIONS: Scrubs reduced the spread of airborne S. aureus, independent of the presence of a rhinovirus-induced cold. Airborne dispersal of S. aureus during sessions in which participants wore surgical scrubs was not significantly different from that during sessions in which gowns and gowns plus masks were also worn. 相似文献
26.
Geoffrey Thiele Molly Bicak Helen Grierson Patrick Lai David Purtilo 《Journal of immunological methods》1987,100(1-2):249-259
An enzyme-linked immunosorbent assay (ELISA) was used to measured IgG antiboody titers againt a synthetic peptide whose sequence was derived from the glycine-alanine repeating region of Epstein-Barr virus nuclear associated antigen 1 (EBNA-1). Antibody titers were determined in sera from 15 normal subjects, sera from 21 normal male siblings of X-linked lymphoproliferative syndrome (XLP) patients, from 20 XLP patients comprising a total of 42 samples, and ten samples before and ten samples after gamma-globulin therapy in ten patients with XLP. Data analysis demonstrated that while there are differences between the ELISA and ACIF, they appear to measure a similar response as demonstrated by their correlation coefficient (0.77) and the GMT to EBNA observed by both methods. No cross-reactivity of cytomegalovirus antibodies to the EBNA-1 peptide was observed by immunobv using adsorption against AD-169 infected MRC-5 cells.. However, non-specific binding was observed if samples were not pre-incubated in a 10% goat serum PBS-Tween 20 solution. This pre-treatment removed the non-specific binding that falsely elevated GMT in approximately 15% of both normal and XLP samples in ELISA. The ELISA system appears to be a sensitive, reproducible and objective test that may be useful for assessing the antibody responses of patients to the EBNA-1 protein. 相似文献
27.
28.
29.
Electrophysiological evidence of P2X2 receptor expression in the neurons of intracardiac and paratracheal ganglia 总被引:1,自引:0,他引:1
Objective: To investigate the expression of P2X receptors on rat intracardiac and paratracheal ganglion neurons. Methods: For preparation of intracardiac neurons, hearts were excised, the atria were separated and the medial region containing intracardiac ganglia was isolated and cut into pieces. For preparation of paratracheal neurons, the tracheas were removed and the superficial membranous layer containing paratracheal ganglia was rapidly isolated. Intracardiac and paratracheal ganglion neurons were dissociated after digestion by collagenase and trypsin. Whole-cell patch clamp recording was used to identify the pharmacological properties of P2X receptors in cultured neurons. Results:Neurons from these two ganglia responded to ATP with a rapidly activating, sustained inward current, αβ-meATP failed to evoke any re- sponses in paratracheal ganglion neurons while a few of intracardiac ganglion neurons responded to αβ- meATP with a tiny sustained inward current. ADP and UTP had no effect on intracardiac neurons. Lowering pH potentiated ATP responses in neurons from these two ganglia whereas increasing pH inhibited ATP responses. Co-application of Zn^2+ potentiated ATP responses in intracardiac and paratracheal ganglion neurons. Conclusion: The receptor subtypes involved in intracardiac and paratracheal ganglia appear to be homomeric P2X2, while heteromeric P2X2/3 could not be completely excluded from intracardiac neurons. 相似文献
30.