视盘血管炎15例报告

视盘血管炎１５例报告广州部队武汉部医院眼科金中秋，许亚宏，尹禾武汉市六七二医院眼科曾祥冰本组报告１５例Ｉ６眼视盘血管炎，其中１型即视神经乳头水肿型４眼，１１型即视网膜静脉阻塞型１２眼。所有病例进行了视力、眼底及荧光造影检查．属于１１型的患者进行了血压...     

碘盐防治对改善缺碘地区儿童智力的调查分析

众多资料研究证实缺碘地区儿童智力明显低于非缺碘地区。为了解碘盐防治后缺碘地区儿童智力水平,验证防治效果,对我县实施碘盐防治措施20余年的缺碘地区什字乡(下称防治区)学龄儿童进行了智力调查,并以未经防治的缺碘地区石岔乡(下称未防治区)和非缺碘地区黄锋堡乡作对照。对象和方法一、一般情况:什字乡位于六盘山东侧腹地(?)区。是原地甲病重病区。水碘范围0.1～     

职业性锰暴露工人健康状况调查分析

对１８２名职业性暴露于锰的电焊工人和１５４名无锰及其它重金属暴露史的对照工人进行健康状况调查，发现暴露组神经系统改变发生率为２６．９６，比数比为２．６２，并且锰暴露年数与神经系统改变呈剂量－反应关系。     

共同性斜视术后的回退问题

共同性斜视矫正术后,近期为双眼正位,但时间长后又能出现以下几种变化:①眼位回退到原斜视方向,②眼位向原斜视方向的相反方向移行;③保持双眼正位。如果我们能将引起眼位变化的因素加以分析,以指导我们手术,会提高斜视矫正手术成功率。现我们将我院的48例共同性斜视(共同性外斜30例,共同性内斜18例)矫正术3年后的情况报告如下:对象和方法一、一般资料;本组病例系1987年12月     

天蚕素A-蛙皮肽杂合肽基因的表达及产物抗菌活性测定

目的 研究天蚕素A-蛙皮肽杂合肽基因[CA（1-8）-MA（1-12）]抗菌肽的抗菌活性。方法 将天蚕素A-蛙皮肽杂合肽基因抗菌肽的人工基因克隆到具有自切割功能的表达载体pTYB12上，并在大肠杆菌（E.coli)BL21(DE3)中进行表达，表达产物用几丁质树脂进行了亲和吸附，自切割后洗脱得到抗菌肽，进行透析以去除盐类物质，最后用大肠杆菌与金黄色葡萄球菌进行活性检测。结果 天蚕素A-蛙皮肽杂合肽[CA（1-8）-MA（1-12）]对大肠杆菌与金黄色葡萄球菌均有一定的抗菌活性。结论 天蚕素A-蛙皮肽杂合肽具有抗菌活性。其抑瘤活性的研究还有待于进一步研究。     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

不同门奇断流术式对门静脉高压症犬食管下段血管再生的影响

Objective To explore the effect on blood vessel regeneration of distal esophagus in ca-nines of portal hypertension with liver cirrhosis after different procedures with paraesophagastrie devascular-ization. Methods Portal hypertension models were produced in canines by subcutaneous injection of 60% CC14 combined with food restriction. Forty eight model canines were randomly divided into 4 groups: group A, traditional paraesophagastrie devascularization;group B, selective paraesophagastric devascularization;group C, paraesophagastric devascularization plus distal esophageal transaction and reanastomosis with sta-pler;group D,control group. Every group had 12 canines which were randomly divided into A1 ,B1 ,C1 ,D1, A2,B2,C2 and D2 groups after the operation. Canines in groups A1 ,B1 ,C1 and D1 were executed at first month after the operation,and those in groups A2, B2, C2 and D2 were executed at the 6th month after the operation. The distal esophagus was segmented when the canines were executed. The upside and downside of the stoma in group C were distinguished and signed as Ca and Cb. Immunohistochemieal methods were used to detect VEGF,CD34 and FVⅧ-Rag,blood vessel-related factors,in lower esophagus. Results The expres-sion levels of VEGF and CD34 MVD,FⅧ-Rag MVD in groups A1 ,B1 and C1 were lower than those in group D1 one month after the operation (P <0.05). All the indices in group B1 were lower than those in groups A1 and Clb,but higher than those in group Cla (P <0.05). The indices in group Cla were lower than those in group C1b (P < 0.05 ). The indices in groups A2 and B2 were higher than those in groups A1 and B1, re-spectively at the 6th month after the operation (P <0.05). They were higer in outer membrane in group C1a than in group C2a (P <0.05). The indices in group C2b were higher than those in group Clb (P<0.05). Conclusion At the first month after operation, the expression of blood vessel-related factors was declined in three different procedures of paraesophagastric devascularization,which indicated that all three different pro-cedures could diminish the vessels in distal esophagus. The effect in group C was most obvious among all groups. At the 6th month after traditional paraesophagastrie devascularization and selective paraesophagastrie devascularization, the expression of blood vessel-related factors in distal esophagus was declined. But there were no significant changes in the expression of blood vessel-related factors in distal esophagus after parae-sophagastric devascularization plus distal esophageal transaction and reanastomosis with stapler. It indicated that the blood vessel regeneration in paraesophagastric devascularization pins distal esophageal transaction and reanastomosis with stapler was inferior to that in other procedures.     

山丘型地区消灭血吸虫病后彻底灭螺价值的探讨

消灭钉螺的目的是为了消灭血吸虫病。但是,已经消灭血吸虫病的地区,是否还要彻底灭螺,这是当前防治血吸虫病工作中迫切需要解决的重要课题。消灭血吸虫病标准规定:“在流行地区范围内,需要连续三年查不到钉螺”才算消灭,因此,不少地区虽然早已消灭血吸虫病,仍然对残存钉螺或已经无螺但环境仍然适合钉螺孳生的广大地区,全面开展预防性的药物灭螺,不但造成药物的严重浪费,而且污染环境,破坏生态平衡,这种作法是否还要坚持下去,值得慎重考虑。为了探讨防