大连市肠道病毒71型基因特征分析

目的 了解大连市手足口病病原体肠道病毒71型(EV71)的VP1区段的基因特征.方法 用RT-PCR方法从手足口病患者临床标本中扩增EV71的VP1基因全序列,利用软件进行同源性分析和构建系统发生树.结果 测序获得的9株病毒基因与C4亚型代表株具有较高的核苷酸和氨基酸序列同源性.在系统进化树上,9株病毒基因与C4基因亚型代表株处于同一分支.结论 大连市EV71病毒株属C4亚型.     

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??Abstract??Objective??To study the changes of shape?? size and signal intensity of pituitary gland in idiopathic growth hormone deficiency??IGHD??in adolescent. Methods??Clinical data of pituitary MRI of 100 puberty with IGHD were chosen from the Endocrine Department of Peking Union Medical College Hospital from January 2005 to January 2010??Compared these results with the normal. Results??There were significant difference of the superior shape of the pituitary and the MRI signal between the study group and control group. The height of pituitary gland and the width of pituitary stalk in the study group were significantly smaller than the control group ??P < 0.01??. There were three major imaging features of the pituitary MRI in IGHD cases?? the pituitary hypoplasia?? the posterior lobe of pituitary dystopia or disappear and the pituitary stalk thinning or disappear. Conclusion??There is a remarkable change of the pituitary in IGHD cases.It is necessary to combine the MRI of the pituitary with the clinical and laboratory findings to diagnose IGHD.     

电针针刺经筋结点结合康复疗法治疗中风后肩关节半脱位的疗效观察

目的:观察电针针刺经筋结点结合康复疗法对中风后肩关节半脱位的治疗效果.方法:采用随机分组的方法,将患者分为治疗组和对照组,每组各20例.两组均进行康复训练,治疗组在康复训练的基础上加电针针刺经筋结点,观察两组治疗效果并进行对比分析.结果:治疗组显效14例,有效5例,总有效率95％,与对照组(75％)比较,有显著性差异(P＜0.05).结论:电针针刺经筋结点结合康复疗法治疗中风后肩关节半脱位疗效明显.     

滋养层细胞表面蛋白2及其在肿瘤中的表达

滋养层细胞表面蛋白2( TROP2)是一种细胞表面糖蛋白,因为仅在滋养层细胞表面高表达而得名.最近研究发现TROP2蛋白在很多人类上皮性肿瘤中也存在高表达,而且与患者的预后有关,但其具体的作用机制目前仍不清楚.     

HLA-G诱导T细胞免疫耐受的实验研究

用W6/ 3 2单抗对JEG 3细胞进行免疫荧光染色 ,可见在JEG 3细胞膜表面有高强度的黄绿色荧光。HLA G+的JEG 3细胞作为刺激细胞 ,观察其对淋巴细胞增殖反应的影响 ,结果JEG 3细胞不能刺激淋巴细胞增殖。采用经典的单向混合淋巴细胞培养的方法 ,以转染及未转染HLA G分子的K5 62细胞作为抑制细胞 ,按一定的比例加入反应体系 ,结果表明 ,转染HLA G的K5 62细胞能抑制淋巴细胞的增殖反应 ,该抑制以刺激细胞∶反应细胞∶抑制细胞的比例为 1∶1∶2时效果最明显 ,抑制率为 5 4 1% (P <0 0 1) ,转染空质粒和未转染HLA G的K5 62细胞均无明显抑制作用。外周血淋巴细胞与JEG 3细胞共同孵育 ,碘化丙啶 (PI)染色 ,流式细胞仪观察 ,结果发现 ,HLA G分子能诱导淋巴细胞的凋亡。     

舒密NW160型物流传输系统的改进

本文针对舒密NW160型物流传输系统在实际使用中遇到的问题,给出了相应的改进方法,取得了较好的效果.     

Septal Dyskinesia and Global Left Ventricular Dysfunction in Pediatric Wolff‐Parkinson‐White Syndrome with Septal Accessory Pathway

LV Dysfunction in WPW Syndrome. Introduction: Echocardiographic studies have shown that some patients with Wolff‐Parkinson‐White (WPW) syndrome have myocardial dyskinesia in the segments precociously activated by an accessory pathway (AP). The aim of the present study was to determine the extent to which the AP contributes to global left ventricular (LV) dysfunction. Methods: Electrophysiological and echocardiographic data from 62 children with WPW (age at diagnosis = 5.9 ± 4.2 years) were retrospectively analyzed. Results: The left ventricular ejection fraction (LVEF) of patients with septal APs (53 ± 11%) was significantly lower than that of patients with right (62 ± 5%) or left (61 ± 4%) APs (P = 0.001). Compared to patients with normal septal motion (n = 56), patients with septal dyskinesia (n = 6) had a reduced LVEF (61 ± 4% and 42 ± 5%, respectively) and an increased LV end diastolic dimension (P < 0.001 for both comparisons). Multivariate analysis identified septal dyskinesia as the only significant risk factor for reduced LVEF. All 6 patients with septal dyskinesia had right septal APs, and a preexcited QRS duration that was longer than that of patients with normal septal motion (140 ± 18 ms and 113 ± 32 ms, respectively; P = 0.045). After RFA there were improvements in both intraventricular dyssynchrony (septal‐to‐posterior wall motion delay, from 154 ± 91 ms to 33 ± 17 ms) and interventricular septal thinning (from 3.0 ± 0.5 mm to 5.3 ± 2.6 mm), and a significant increase in LVEF (from 42 ± 5% to 67 ± 8%; P = 0.001). Conclusion: The dyskinetic segment activated by a right septal AP in WPW syndrome may lead to ventricular dilation and dysfunction. RFA produced mechanical resynchronization, reverse remodeling, and improvements in LV function. (J Cardiovasc Electrophysiol, Vol. 21, pp. 290–295, March 2010)     

A global internet survey of health promotion training

This paper describes a global internet survey, undertaken onbehalf of WHO, Geneva, to determine the nature and extent ofhealth promotion education and training. Forty-three responseswere elicited, mainly from within the European region. Despitedifferences in organisation and levels of training there wassome consistency in the approach and philosophy to the educationprogrammes across the globe. A fuller more comprehensive surveymay help to address some of the issues raised in this research.     

美托洛尔治疗小儿慢性充血性心力衰竭疗效观察

目的 探讨美托洛尔治疗小儿慢性充血性心力衰竭的疗效.方法 在常规应用强心、利尿、扩血管药物及对症治疗基础上,给予患儿加服美托洛尔,从小剂量[0.2～0.5 mg/(kg·d)]开始,4周内达最大量[2mg/(kg·d)].结果 用药3个月时,治疗组总有效率为95%,对照组为56%,两组间差异有显著性(P<0.05).治疗组心率、心胸比率较对照组明显降低,射血分数值明显升高,治疗组疗效优于对照组(P<0.05).结论 常规抗心力衰竭药物加美托洛尔可明显改善慢性充血性心力衰竭患儿心功能.     

Balo病同心圆硬化的MRI、质子波谱和活检病理对照研究

目的 描述Balo病同心圆硬化的MRI和质子波谱（^1H－MRS）特征。方法 报告了3例经活检病理证实的Balo病同心圆硬化的MRI表现，其中1例同时进行了^1H－MRS检查。结果 3例病人中，2例多发、1例单发。病灶均位于中央白质区，呈典型的同心圆形改变，增强扫描呈典型层状“洋葱皮样”增强。 ^1H-MRS示病变区N-乙酰天冬氨酸（NAA）降低、胆碱增高并出现异常脂质峰。结论 Balo氏同心圆硬化MRI和^1H－MRS表现具有特征性，MRI应作为本病的首选检查方法。