鹿鞭线粒体DNA指纹鉴定

目的探讨鹿鞭及牛鞭线粒体细胞色素b基因部分序列片段特征,建立中药材鹿鞭敏感、特异的DNA分子标记学鉴定方法。方法碱变性法提取新鲜鹿鞭及牛鞭,采用引物设计软件PrimerPremier5.0对鹿鞭及牛鞭的细胞色素b基因序列分析,用于鉴定正品鹿鞭的特异性引物,经聚合酶链式反应(PCR)扩增,对鹿鞭进行DNA指纹鉴定。结果对鹿鞭进行mtDNA鉴定图谱显示,真品鹿鞭有306bp条带,伪品没有。结论用此方法对鹿鞭进行鉴定,可准确辨别正品与伪品。重现性、稳定性好,简便准确而可行。鹿鞭mtDNA具有特异性指纹特征,所得鹿鞭DNA指纹特征图谱可用于鹿鞭的鉴定。     

Syk及HER2基因在胃癌组织中的表达及意义

目的:观察脾酪氨酸激酶(Syk)与人表皮生长因子受体2(HER2)蛋白在胃癌组织中的表达,探讨其与胃癌发生发展的关系及其临床意义。方法:用免疫组化法检测55例胃癌及20例癌旁组织中Syk与HER2蛋白表达;分析在不同组织学类型、TNM分期、淋巴结转移、浆膜浸润、远处转移等胃癌组织中Syk及HER2蛋白的表达情况。结果:55例胃癌中Syk、HER2的表达率分别为21.8%(12/55)和29.1%(16/55)。20例癌旁组织中的表达率分别为85.0%(17/20)和0。胃癌组织Syk蛋白表达阳性率显着低于癌旁组织(P<0.01);而HER2蛋白在胃癌的表达率高于癌旁组织(P<0.01)。Syk、HER2蛋白表达在胃癌的不同组织学分类、肿瘤TNM分期以及有无淋巴结转移、浆膜浸润和远处转移差异有统计学意义(P<0.05),而不同年龄、性别间差异无统计学意义。分化程度越差、浸润越深、出现淋巴结转移及远处转移、TNM分期越高HER2阳性率越高,而Syk则在低分化中出现表达缺失。结论:Syk、HER2基因检测可作为评估胃癌恶性生物学行为及预后的重要指标;并为靶向治疗提供理论依据。     

钬激光治疗输尿管结石并发狭窄的处理策略

目的：探讨腔内钬激光治疗输尿管结石并发狭窄的处理策略.方法：收集36例输尿管镜下钬激光切开治疗输尿管结石并发狭窄患者的临床资料.结果：35例手术成功,1例单纯壁间段狭窄者予钬激光切开后仍无法上行,手术终止.术后共随访12～45个月,平均（23.8±9.5）个月.30例治愈或好转,有效率为83.3%（30/36）.6例无效（5例术后1～3个月结石复发,复行腔内激光手术,随访18～24个月,其中4例治疗有效,1例效果不佳.结论：经尿道钬激光碎石术（TUL）在治疗上尿路结石时可同时处理输尿管狭窄,是一种安全有效的手段.钬激光切开治疗狭窄的疗效（85.2%,23/27）要好于硬性扩张（77.8%,7/9）,但两者比较无统计学意义（P＞0.05）根据术中具体情况实施操作可能更具实用价值,不仅能够减少操作所需时间,且预后理想.我们倾向性认为中下段狭窄的预后（93.1%,27/29）较上段（57.1%,4/7）较好（P＜0.05）,但其处理方法选择标准及处理策略仍有待进一步探讨和完善.     

子宫内膜癌组织中基质金属蛋白酶-7及其组织抑制剂-2的表达

目的探讨子宫内膜癌(EC)组织中基质金属蛋白酶-7(MMP-7)及其组织抑制剂-2(TIMP--2)的表达,分析其与EC侵袭、转移的关系。方法选取64例EC组织标本及同期子宫内膜不典型增生组织标本20例、正常子宫内膜20例为对照组。用免疫组织化学方法检测MMP-7、TIMP-2的表达。结果 MMP-7在EC中阳性表达强度显著高于正常子宫内膜(P0.05);与不典型增生组比较差异无统计学意义(P0.05);MMP-7的阳性表达强度在不典型增生组高于正常内膜组(P0.05);MMP-7与手术-病理分期、肌层浸润深度、组织学分级及淋巴结的转移相关(P0.05)。TIMP-2在EC中阳性表达强度显著高于正常子宫内膜组和不典型增生组(P0.05);正常子宫内膜组和不典型增生组比较TIMP-2的表达无统计学意义(P0.05);TIMP-2仅与有无淋巴结转移有关(P0.05)。MMP-7和TIMP-2在EC组织中的表达呈正相关性(r=0.654,P0.001)。结论 MMP-7与TIMP-2在EC组织中均呈高表达;且子宫内膜不典型增生组MMP-7的表达强度明显高于正常内膜组,可能与EC前病变以及EC的发生发展、浸润和转移相关。     

Clinical Observations on 46 Cases of Globus Hystericus Treated with Modified Banxia Houpu Decoction

Objective:To observe the therapeutic effect and mechanism of modified Banxia Houpu decoction on globus hystericus.Methods:The 95 patients with globus hystericus were randomly divided into a treatment group of 46 cases treated with modified Banxia Houpu decoction and a control group of 49 cases treated with Manyanshuning(Granula for Clearing the Throat).In addition,a normal group of 24 healthy people was set up.SCL-90 scale was adopted to observe the therapeutic effect,evaluate the psychological state of pat...     

Effects of mixture of lidocaine and ropivacaine at different concentrations on the central nervous system and cardiovascular toxicity in rats

Background Lidocaine and ropivacaine are often combined in clinical practice to obtain a rapid onset and a prolonged duration of action. However, the systemic toxicity of their mixture at different concentrations is unclear. This study aimed to compare the systemic toxicity of the mixture of ropivacaine and lidocaine at different concentrations when administered intravenously in rats. Methods Forty-eJght male WJstar rats were randomly divided into 4 groups （n=12 each）： 0.5% ropJvacaine （group Ⅰ）; 1.0% ropivacaine and 1.0% lidocaine mixture （group Ⅱ）; 1.0% ropivacaine and 2.0% lidocaine mixture （group Ⅲ）; and 1.0% lidocaine （group Ⅳ）. Local anesthetics were infused at a constant rate until cardiac arrest. Electrocardiogram, electroencephalogram and arterial blood pressure were continuously monitored. The onset of toxic manifestations （seizure, dysrhythmia, and cardiac arrest） was recorded, and then the doses of local anesthetics were calculated. Arterial blood samples were drawn for the determination of local anesthetics concentrations by high-performance liquid chromatography. Results The onset of dysrhythmia was later significantly in group IV than in group Ⅰ, group Ⅱ, and group Ⅲ （P 〈0.01）, but there was no significant difference in these groups （P 〉0.05）. The onset of seizure, cardiac arrest in group Ⅰ （（9.2±1.0） min, （37.0±3.0） min） was similar to that in group Ⅱ（（9.1±0.9） min, （35.0±4.0） min） （P 〉0.05）, but both were later in group Ⅲ （（7.5±0.7） min, （28.0±3.0） min） （P 〈0.05）. The onset of each toxic manifestation was significantly later in group Ⅳ than in group Ⅰ （P 〈0.01）. The plasma concentrations of the lidocaine-alone group at the onset of dysrhythmia （DYS）, cardiac arrest （CA） （（41.2±6.8） min, （59.0±9.0） min） were higher than those of the ropivacaine alone group （（20.5±3.8） min, （38.0±8.0） min） （P 〈0.05）. The plasma concentrations of ropivacaine inducing toxic manifestation were not significantly different among groups Ⅰ, Ⅱ, and Ⅲ（P 〉0.05）. Conclusions The systemic toxicity of the mixture of 1.0% ropivacaine and 2.0% lidocaine is the greatest while that of 1.0% lidocaine is the least. However, the systemic toxicity of the mixture of 1.0% ropivacaine and 1.0% lidocaine is similar to that of 0.5% ropivacaine alone.     

CT增强延时扫描在重复肾输尿管诊断中的应用

目的探讨CT增强延时扫描对重复肾、输尿管的诊断价值及临床应用。方法16例重复肾、输尿管的患者行多层螺旋CT平扫、增强、延迟扫描,采用三维重建技术对原始数据进行重建,获得泌尿系统立体图像。结果CT增强、延时扫描能够明确显示重复肾、输尿管结构、位置和功能。结论CT增强延时扫描对重复肾、输尿管诊断及临床治疗具有重要的应用价值。     

重复性异位妊娠56例临床分析

目的探讨重复异位妊娠发病的病因及预防。方法回顾性分析56例重复异位妊娠的妊娠次数及首次异位妊娠盆腔情况、发生部位、治疗方法与重复异位妊娠的关系。结果56例重复异位妊娠中妊娠次数≤3次9例,5例妊娠次数多于6次,97．73％存在盆腔拈连。92．59％输卵管切除后重复异位妊娠发生在对侧,而保守治疗后重复异位妊娠仅20．69％发生在对侧。两者问差异有显著性（P〈0．001）。结论重复异位妊娠的发生与妊娠次数,输卵管炎症及前次异位妊娠的治疗方法有关。     

铁代谢标志物与2型糖尿病的关系:基于中国健康和营养调查数据的分析

目的:探讨铁代谢标志物(血清铁蛋白、可溶性转铁蛋白受体、血红蛋白和转铁蛋白)与2型糖尿病(T2DM)的关系.方法:基于中国健康和营养调查2009年的调查数据,最终纳入8337名成年人作为研究对象,使用Logistic回归分析铁代谢标志物与T2DM之间的关系.进行性别分层,探讨不同性别人群铁代谢标志物水平与T2DM患病风...