异基因外周血干细胞移植治疗苯中毒性再生障碍性贫血

目的　研究异基因外周血造血干细胞移植治疗苯中毒致重型再生障碍性贫血 (SAA)。方法　用HLA配型全相合的女性供者异基因外周血造血干细胞移植 (Allo PBSCT)治疗 1例苯中毒致SAA男性患者。输入单个核细胞数为 9.4 1× 10 8 kg、CD34 阳性细胞为 12 .4 9× 10 6 kg、粒 -巨噬细胞系祖细胞集落数为 8.2× 10 5 kg。预处理方案由环磷酰胺加全身照射和抗淋巴细胞球蛋白组成 (环磷酰胺总量 12 0mg kg ,全身照射为 8Gy ,抗淋巴细胞球蛋白总量 6 0mg kg)。为防止植入失败 ,移植后第 18天再次输入供者白细胞层 ,其中单个核细胞数为 9.0 2× 10 8 kg、CD34 阳性细胞为 10 .6 2× 10 6 kg、粒 -巨噬细胞系祖细胞集落数为 6 .3× 10 5 kg,并用环孢菌素A和甲氨蝶呤预防移植物抗宿主病。结果　输入单个核细胞后白细胞最低值为 0 ,血小板最低值为 3× 10 9 L ;中性粒细胞于移植后第 2 1天开始大于0 .5× 10 9 L ,血小板于移植后第 2 8天开始大于 5 0× 10 9 L。移植后第 4个月出现皮肤局限性慢性移植物抗宿主病。受者染色体转为 4 6 ,XX ;ABO血型由B型转为O型。结论　此例为国内首例采用Allo PBSCT治疗苯中毒致SAA并获得成功 ,为苯中毒治疗开辟了新的途径。     

广州市直管厂职业健康监护结果宏观分析

广州市直管厂职业健康监护结果宏观分析刘薇薇本文就１９９３～１９９５年广州市直管厂职业健康监护进行分析如下。１对象与方法１．１对象随机抽出２６４间工厂，选择１９９３～１９９５年广州市直管厂有害作业工人体检中的资料，三年各有１６１２９人，１６４４９人和２...     

利用Ovid平台整合网络资源的方法

介绍了Ovid平台为用户所提供的网络资源链接技术Link Solver的使用方法.通过这一技术的使用,图书馆可以根据本馆用户的需求特点,对各种网络资源进行有效的整合,从而帮助用户方便地获取所需信息.     

从美国《医学索引》版本谈馆藏检索工具的订购策略

本文探讨了停购IM的可行性,指出在维持印刷版检索工具的同时,还应扩大利用已引进的MEDLINE光盘数据库.在检索工具电子版发展速度很快、印刷版还要并存相当长时期的今天,图书馆如何对待这两种类型的检索工具值得探索.     

基础医学整合课程教学效果评价的公因子分析

目的　提取当前我国基础医学整合课程教学效果评价的公因子,分析和解释公因子的实际意义。方法　对我国15所高等医学院校的本科学生进行基础医学整合课程教学效果评价的问卷调查,采用探索性因子分析法进行筛选和归类。利用SPSS 22.0统计软件进行因子分析。结果　评价整合课程教学效果的四大公因子分别是教学过程的有效性、教学组织的合理性、教学内容的教育性、教学方法的创新性。结论　基础医学整合课程改革应高度关注基础理论学习与实践能力培养的同步进行,注重理论教学与实验教学的有效衔接,重视对学生综合素质的教育作用,灵活运用多种教学手段和方法。     

丹参中主要活性成分生物合成的研究进展

丹参酮类和丹参酚酸类化合物是丹参中两类主要的活性成分,其含量水平是丹参药效的决定因素,直接体现了药材的品质。为深入解析丹参的品质内涵以提升丹参品质,学者们开展了一系列研究以阐明丹参中活性成分的生物合成机制及累积特征。近年来,随着更高效的基因组、代谢组等研究手段的应用,丹参活性成分的生物合成研究获得了快速发展,本研究对该领域研究进展进行综述,为丹参的活性成分及其药材品质的深入研究提供理论依据。     

Study on the mechanisms of electroacupuncture for promoting gastrointestinal motility in rats with diabetic gastroparesis

Objective

To observe the effect of electroacupuncture (EA) at Zusanli (ST 36) on the gastrointestinal motility and the ultrastructures of pacemaker cells [the interstitial cells of Cajal (ICC)] in diabetic gastroparesis (DGP) rats and explore the mechanism of EA for DGP.

Methods

A total of 50 Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, with 10 rats in each group. Group A was the blank control; a single intraperitoneal injection of 2% streptozotocin (STZ) was performed in rats of group B, group C, group D and group E, with high glucose and high fat diet for 8 weeks to establish the DGP rat models. Group B was the model group and the rats did not receive any treatment; group C was EA at acupoint group and the rats received EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); group D was EA at non-acupoint group and the rats received EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); group E was metoclopramide group and the rats were treated by intragastric administration of metoclopramide. Blood glucose was detected using ONE TOUCH blood glucose meter; gastric emptying rate and small intestine migration rate were measured using intragastric phenol red; ultrastructures of gastric antrum ICC were detected by transmission electron microscopy.

Results

The differences of blood glucose between group B, group C, group D, group E versus that of group A were statistically significant after modeling (P<0.01); after treatment, the differences of blood glucose between group D, group E versus that of group C were statistically significant (P<0.05, P<0.01); the gastric emptying rate of rats in group B was statistically significant different from that in group A (P<0.01); the gastric emptying rate of rats in group C was statistically significant different from that in group B (P<0.01). The migration rates of rats’ small intestines in group B, group C, group D and group E were all statistically significant different from that in group A (P<0.01); the migration rate of rats’ small intestines in group C was statistically significant different from that in group B (P<0.01). The ultrastructure of rat’s ICC in group B showed apoptosis compared with that in group A; rat‘s ICC in group C had complete basement membrane, more cytoplasm mitochondria, Golgi and rough endoplasmic reticulum, showing clear structure, occasional mitochondria swelling and gap junctions with adjacent smooth muscle cells; there were no significant differences between group D, group E versus group B.

Conclusion

EA at Zusanli (ST 36) plus other acupoints can regulate the blood glucose and promote gastrointestinal motility in DGP rats, and the mechanism may be related to repairing the damaged ICC structure.

     

基于经典测量理论和项目反应理论的妇幼保健机构患者安全文化量表研究

目的编制出适用于妇幼保健机构的患者安全文化量表。方法于2016年1-2月,在北京市的3个区县级妇幼保健机构对429名医务人员进行问卷调查,采用基于经典测量理论和项目反应理论的13种统计方法筛选条目。结果量表被调整至11个维度(含46个条目),各维度的克朗巴赫α系数在0.525-0.896。结论经典测量理论和项目反应理论各有优劣势,应结合使用;该量表可用于评价妇幼保健机构的患者安全文化水平。