Effects of gliclazide on myocardial protection of isolated type 2 diabetic rat heart afforded by ischemic preconditioning

The myocardial protection afforded by ischemic preconditioning(IPC) can alleviate ischemia-reperfusion injury in normal rat heart.However,this myocardial protection is seldom studied in the type 2 diabetic rat with myocardial ischemia disease.In this study,we aimed to evaluate the effects of ATP-sensitive potassium channels(KATP channels) on IPC in the isolated type 2 diabetic rat heart and the role of the sulfonylurea gliclazide.Methods Streptozotocin(STZ)-induced type 2 diabetic male Wistar rats with or without gliclazide(64 mg /kg body weight,orally) and age-matched non-diabetic control rats were used for all studies.The isolated hearts were perfused with Langendorff’s system under the constant flow,pressure and temperature conditions with Kreb’s-Henseleit solution(K-H).After 5 minutes of balance perfusion,these rats were randomly divided into six groups:non-diabetic control rats without IPC(CIR);non-diabetic control rats with IPC(CIP);diabetic rats without IPC(DIR);diabetic rats with IPC(DIP);gliclazide-treated diabetic rats without IPC(GIR);and gliclazide-treated diabetic rats with IPC(GIP).Groups CIR,DIR,and GIR were subjected to 30-min global ischemia and 60-min reperfusion for induction of ischemia /reperfusion injury.Groups CIP,DIP,and GIP were given three cycles of 5-min ischemia and 5-min reperfusion as IPC,and then ischemia /reperfusion injury program was implemented.Extent of ischemia /reperfusion injury was measured in terms of the release of lactate dehydrogenase(LDH),creatine kinase(CK),and creatin kinase-MB(CKMB) in coronary effluent.After perfusion,Kir6.2 and SUR2A mRNA expressions in the myocardial tissue were characterized by fluorescent quantitative real-time PCR method,and Kir6.2 and SUR2A protein expressions were assessed by immunohistochemistry.Result In non-diabetic control rats,the release of LDH,CK,and CK-MB in coronary effluent markedly decreased with IPC compared with No-IPC(P < 0.05),but not in diabetic rats.However,in gliclazide-treated diabetic rats,IPC-induced decrease in the release of LDH,CK,and CK-MB was restored compared with No-IPC(P < 0.05).The expressions of Kir6.2 both at mRNA and protein levels in CIP were significantly higher than those in CIR.There was no significant difference in the expression of Kir6.2 and SUR2A both at mRNA and protein levels between DIP and DIR.However,the expression of Kir6.2 both at mRNA and protein levels was significantly higher in GIP than in GIR.No significant difference was detected in the mRNA expression level of SUR2A between the six groups.The expression of SUR2A at protein level was significantly higher in CIP than in CIR and in GIP than in GIR.Conclusions The cardioprotective effect of IPC is abolished in the isolated type 2 diabetic rats compared with non-diabetic control rats.However,to some extent,gliclazide can improve the myocardial protection of IPC against ischemia /reperfusion injury,thus suggesting that it is mediated mainly by KATP channels at mRNA or protein level,which provides a basis for further investigating the effects of KATP channels on IPC.     

罕见的胸壁Askin瘤一例

患者女,13岁。自1999年7月初在无诱因下感右背部阵发性刺痛,向右肩背部放射,但无发热、咳嗽、疲乏、盗汗、咯血、气促等症状。体检:发育正常,无病容。浅表淋巴结未及,右背部在第7～8后肋部稍隆起,可扪及肿块,质硬、轻压痛、无波动感。右背部隆起处叩音浊,呼吸音下降,其他无异常。实验室检查:除碱性磷酸酶(261Ｕ/Ｌ)和乳酸脱氢酶(384Ｕ/Ｌ)异常增高外,余均在正常范围。Ｘ线肺后前位平片示右肺中下部有一巨大圆形阴影,在圆形阴影下的肺纹、肺门血管阴影均清晰可辨,无移位现象,肿块与右心缘分开,余肺野及膈角均正常。右侧位片示肿块呈Ｄ形,紧贴…     

奉贤县举行第十届健康教育周活动

上海地区虽不是肺吸虫病流行区，但近年来本地区盛行进食海鲜，不少酒楼、饭店出售呛虾、呛蟹。有些虾、蟹、淡水螺等是肺吸虫的第一或第二宿主，人们进食这些含有囊蚴的生虾、生蟹而感染肺吸虫病。本报告5例极喜食呛虾、呛蟹在3个月内多次进食各种海鲜(包括呛虾、呛蟹)，在1个月前后发病，现将病情报告如下：     

横U形钢丝捆扎配合镍钛记忆合金髌骨爪治疗髌骨粉碎性骨折的临床疗效

目的 探讨横U形钢丝捆扎配合镍钛记忆合金髌骨爪治疗髌骨粉碎性骨折的临床疗效.方法 将52例髌骨粉碎性骨折患者分别采用单纯镍钛记忆合金髌骨爪(对照组)和横U形钢丝捆扎配合镍钛记忆合金髌骨爪(研究组)治疗.比较两种术式治疗粉碎性髌骨骨折的临床优良率及骨块移位率.结果 研究组临床治疗优良率为96.15％,明显高于对照组的84.62％(χ2=3.97,P＜0.05).研究组骨块移位率为3.85％,明显低于对照组的23.08％(χ2=4.13,P＜0.05).结论 横U形钢丝捆扎配合镍钛记忆合金髌骨爪治疗髌骨粉碎性骨折能达到牢固固定,符合生物固定治疗理念,满足早期功能锻炼,减少术后并发症,尽快恢复膝关节功能.     

重视医学人文教育 培养全面发展人才

时代的进步,社会的发展,医学科技的突飞猛进培养了一批又一批高精尖的专业人才,专业知识和技能却仅能成就一个只会行医看病的"人",而非"医者"。当前,医患关系日趋紧张,而就目前医学院校的医学生培养模式来看,人文教育存在着明显不足,培养全面发展的高素质医学人才刻不容缓,而关键在于人文素质教育与专业教育的内在统一。医学研究和服务的对象是有血有肉,有灵魂、思想、情感的人,医学为解除人类的病痛、使人类能够拥有由内而外的喜悦和幸福感而诞生,医学的发展不仅为了延长寿命更是为了提高生活质量,因此,医学和人文的脱离势必不利于整个人类的可持续发展,实行医学与人文相统一的全方位的教育改革势在必行,以社会主义核心价值观为导向,将医学人文教育贯穿医学人才培养的始终,渗透到医疗行为的方方面面,以期实现人性化的医疗。     

高效液相色谱法测定卡托普利缓释片中卡托普利含量

目的建立测定卡托普利缓释片中卡托普利缓释片含量的高效液相色谱法。方法色谱柱为Agilent Eclipse XDB-C18柱(250 mm×4.6 mm,5μm),0.01 mol/L磷酸二氢铵水溶液(用磷酸调p H至3.0±0.05)-甲醇-乙腈(65∶25∶15)为流动相,流速为1.0 m L/min,检测波长为215 nm。结果卡托普利质量浓度在0.2~2.0μg/m L范围内与峰面积线性关系良好(r=0.999 9),平均回收率为99.34%,RSD=0.52%(n=9)。结论该方法样品分离度效果好,测定结果准确、可靠、重复性好,可用于卡托普利缓释片中卡托普利的含量测定。     

鳞毛蕨属植物中间苯三酚类化学成分质谱裂解和波谱学规律

鳞毛蕨属Dryopteris Adanson植物中的间苯三酚类化学成分因具有抗病毒和抗肿瘤等药理作用,而备受关注。通过对国内外文献研究与分析,对鳞毛蕨属植物中间苯三酚类化学成分的质谱裂解数据和波谱学规律等方面进行了较全面的综述,为该类化学成分的结构鉴定提供了参考。     

肺错构瘤

病历摘要患者男性 ,77岁 ,农民。左前胸稳痛及不适 3日。近 3日来在无明显诱因下感觉左前胸隐痛及呼吸不畅 ,疼痛与咳嗽无关联 ,呈持续性 ,但无缓解期。近多年来除略有咳嗽(嗜烟 )外 ,无咯血、痰血、胸闷等症状 ,亦无类似现象发生过。 6年前因咳嗽、咯血检查发现右上肺结核 ,当时痰液结核捍菌检查为阳性 ,经规范化抗痨治疗 6个月 ,痰菌阴转 ,情况良好 ,此后未再复查过。有嗜烟史 5 0多年 (80 0支 /年 )。同时嗜酒 (黄酒、啤酒 ) ,但量不多。既往除偶有感冒外无其他疾病史。入院体检 :体温 :37℃ ;心律 :80次 / min,律齐 ;呼吸 :2 0次 /min;…     

抗丙型肝炎病毒抗体胶体金免疫层析法的建立

目的研制一种检测丙型肝炎病毒(Hepatitis C Virus,HCV)血清抗体的胶体金双抗原夹心免疫层析检测法。方法将丙肝重组抗原与胶体金连接形成免疫金,质控线与检测线分别包被HCV单抗与丙肝重组抗原,在样品垫上滴加10μl血清和90μl PBS溶液展开。通过加入6种非丙型肝炎阳性血清测试试纸条的特异性;将试纸条用国家血清盘质控品进行灵敏度测试;放置3种温度环境下10周,每周进行2次抽检,进行稳定性测试。结果本检测方法特异性、灵敏度且稳定性均较好,并可在10~15 min内判读结果,与酶联免疫法检测相比,检验符合率能达到较高水平。结论该法能够快速检测人血清中抗HCV抗体。