Dynamic observation on IgG and its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis

Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Era). Methods Forty Balb/c mice of 12-14 week old and 20-25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and IgG2b in the sera of mice increased obviously on 2-18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095±0.033,0.022±0.001,0.023±0.003 respectively, as compared with the value on 0 week(0.030±0.013,0.012±0.004,0.013±0.004), the difference being statistically significant(q=2.95,4.87,2.81 respectively, P < 0.01 or P < 0.05); levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2-18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031±0.004,0.136±0.002,0.114±0.002 respectively, as compared with the value on 0 week(0.192±0.007, 0.175±0.013,0.024±0.003), the difference being statistically significant (q =5.16,4.93,5.32 respectively, P < 0.01 or P < 0.05). Conclusion Helper T cell(TH) Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.     

细粒棘球绦虫重组BCG-Eg95疫苗对感染小鼠脾细胞凋亡的影响

目的:探讨细粒棘球绦虫重组BCG-Eg95疫苗免疫和Eg原头节攻击后小鼠脾细胞凋亡的变化.方法:将细粒棘球绦虫重组BCG-Eg95疫苗采用皮下注射、鼻腔内接种、口服灌胃和肌肉注射4种途径分别免疫BALB/c鼠,免疫后8周用Eg原头节进行攻击感染,感染后18周杀鼠取脾,分离脾细胞,流式细胞仪检测脾细胞的凋亡发生率,同时设有BCG和PBS对照.结果:疫苗接种组的脾细胞凋亡发生率明显低于感染对照组;口服和肌肉注射组的脾细胞凋亡发生率显著低于皮下和鼻腔内接种组.结论:Eg原头节感染可引起小鼠脾细胞发生凋亡,细粒棘球绦虫重组BCG-Eg95疫苗接种可抑制感染鼠脾细胞的凋亡,疫苗口服和肌肉注射是两种较好的免疫途径.     

Dynamic observation on IgG and its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis

Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Era). Methods Forty Balb/c mice of 12-14 week old and 20-25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and IgG2b in the sera of mice increased obviously on 2-18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095±0.033,0.022±0.001,0.023±0.003 respectively, as compared with the value on 0 week(0.030±0.013,0.012±0.004,0.013±0.004), the difference being statistically significant(q=2.95,4.87,2.81 respectively, P < 0.01 or P < 0.05); levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2-18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031±0.004,0.136±0.002,0.114±0.002 respectively, as compared with the value on 0 week(0.192±0.007, 0.175±0.013,0.024±0.003), the difference being statistically significant (q =5.16,4.93,5.32 respectively, P < 0.01 or P < 0.05). Conclusion Helper T cell(TH) Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.     

Dynamic observation on IgG and its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis

Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Era). Methods Forty Balb/c mice of 12-14 week old and 20-25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and IgG2b in the sera of mice increased obviously on 2-18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095±0.033,0.022±0.001,0.023±0.003 respectively, as compared with the value on 0 week(0.030±0.013,0.012±0.004,0.013±0.004), the difference being statistically significant(q=2.95,4.87,2.81 respectively, P < 0.01 or P < 0.05); levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2-18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031±0.004,0.136±0.002,0.114±0.002 respectively, as compared with the value on 0 week(0.192±0.007, 0.175±0.013,0.024±0.003), the difference being statistically significant (q =5.16,4.93,5.32 respectively, P < 0.01 or P < 0.05). Conclusion Helper T cell(TH) Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.     

Dynamic observation on IgG and its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis

Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Era). Methods Forty Balb/c mice of 12-14 week old and 20-25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and IgG2b in the sera of mice increased obviously on 2-18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095±0.033,0.022±0.001,0.023±0.003 respectively, as compared with the value on 0 week(0.030±0.013,0.012±0.004,0.013±0.004), the difference being statistically significant(q=2.95,4.87,2.81 respectively, P < 0.01 or P < 0.05); levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2-18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031±0.004,0.136±0.002,0.114±0.002 respectively, as compared with the value on 0 week(0.192±0.007, 0.175±0.013,0.024±0.003), the difference being statistically significant (q =5.16,4.93,5.32 respectively, P < 0.01 or P < 0.05). Conclusion Helper T cell(TH) Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.     

Dynamic observation on IgG and its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis

Objective To dynamically observe changes of IgG, its subclasses and IgE in sera of mice by immunization with mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis (Era). Methods Forty Balb/c mice of 12-14 week old and 20-25 g weight were intranasally vaccinated by the vaccine, 4 mice were killed randomly by the weight on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, sera were gathered from the eyeball to measure IgG, its subclasses and IgE by routine ELISA. Results Levels of IgG, IgG2a and IgG2b in the sera of mice increased obviously on 2-18 weeks, reached the highest level on 10, 4 and 4 weeks respectively, the value was 0.095±0.033,0.022±0.001,0.023±0.003 respectively, as compared with the value on 0 week(0.030±0.013,0.012±0.004,0.013±0.004), the difference being statistically significant(q=2.95,4.87,2.81 respectively, P < 0.01 or P < 0.05); levels of IgG1, IgG3 and IgE in the sera of mice decreased remarkably on 2-18 weeks,came to the lowest level on 4,2,6 weeks respectively, the value was 0.031±0.004,0.136±0.002,0.114±0.002 respectively, as compared with the value on 0 week(0.192±0.007, 0.175±0.013,0.024±0.003), the difference being statistically significant (q =5.16,4.93,5.32 respectively, P < 0.01 or P < 0.05). Conclusion Helper T cell(TH) Ⅰ response is induced in mice by mixed recombinant of BCG-Em Ⅱ/3 and BCG-Em14-3-3 vaccine on early immunization.     

细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后脾细胞亚群的动态观察

目的 动态观察细粒棘球绦虫重组BCG-Eg95疫苗免疫小鼠后脾细胞亚群的变化.方法 将120只Balb/c小鼠按体质量随机分为3组:鼻腔内接种组、口服灌胃组、对照组.将疫苗分别采用鼻腔内接种和口服灌胃免疫实验组小鼠,对照组单次接种10μl磷酸盐缓冲液(PBS)于小鼠鼻腔黏膜.在免疫后0、2、4、6、8、10、12、14、16、18周各剖杀4只小鼠,取脾脏,分离脾细胞,流式细胞仪检测脾CD4+、CD8+T淋巴细胞亚群的百分比.结果 不同组的小鼠脾细胞CD4+、CD8+亚群百分比比较差异有统计学意义(F值分别为21.56、22.08,P＜0.05),不同周数的小鼠脾细胞CD4+、CD8+亚群百分比比较差异有统计学意义(F值分别为5.75、6.29.P＜0.01).鼻腔内接种组的脾CD4+、CD8+T细胞亚群分别在免疫后6-18、12周升高,在免疫后10、12周达最高水平,其值分别为0.348±0.013、0.090±0.003.与0周(0.230±0.022、0.069±0.015)比较差异有统计学意义(q值分别为7.32、5.32,P＜0.01或＜0.05);口服灌胃组脾细胞CD4+、CD8+T细胞亚群分别在免疫后6～16、8-18周增加,分别在免疫后10、16周达最高水平,其值分别为0.405±0.006、0.096±0.004,与0周(0.230±0.022、0.069±0.015)比较差异有统计学意义(q值分别为7.53、5.35,P＜0.01或＜0.05).结论 CD4+、CD8+T细胞亚群在细粒棘球绦虫重组BCG-Eg95疫苗诱导的小鼠免疫应答中起重要作用.     

多房棘球绦虫混合重组BCG-Em Ⅱ/3和BCG-Em14-3-3疫苗免疫小鼠后IgG及其亚类和IgE的动态观察

目的 动态观察多房棘球绦虫混合重组BCG-Em Ⅱ/3和BCG-Em14-3-3疫苗免疫小鼠后IgG及其业类和IgE的变化.方法 12～14周龄、体质量20～25 g的雌性Balb/c小鼠40只,鼻腔内接种上述疫苗,在0、2、4、6、8、10、12、14、16和18周按体质量随机剖杀4只小鼠,经眼球取血,常规酶联免疫吸附试验法测定血清中IgG及其亚类和IgE水平.结果 Balb/c小鼠的血清IgG、IgG2a和IgG2b水平均在免疫后第2～18周升高,并分别在免疫后第10、4和4周达到最高水平,其值分别为0.095±0.033、0.022±0.001和0.023±0.003,与0周(0.030±0.013、0.012±0.004和0.013±0.004)比较,差异有统计学意义(q值分别为2.95、4.87和2.81,P<0.05或<0.01);血清IgG1、IgG3和IgE水平均在免疫后2～18周降低,并分别在免疫后4、2和6周达较低水平,其值分别为0.031±0.004、0.136±0.002和0.114±0.002,与0周(0.192±0.007、0.175±0.013和0.024±0.003)比较,差异有统计学意义(q值分别为5.16、4.93和5.32,P<0.05或<0.01).结论 多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗在免疫早期即可诱导小鼠产生辅助性T细胞(TH)Ⅰ型保护性免疫反应.     

多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗抑制小鼠脾细胞的凋亡

目的:多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠并以Em原头节攻击后探讨以上疫苗对小鼠脾细胞凋亡的变化.方法:将混合重组BCG疫苗采用皮下注射和鼻腔内接种分别免疫Balb/c鼠8 wk后,用多房棘球绦虫原头节进行攻击感染,感染18 wk杀鼠取脾并分离脾细胞,流式细胞仪检测脾细胞的凋亡发生率,同时设有空载体、BCG和PBS对照.结果:疫苗接种组的脾细胞凋亡发生率明显低于感染对照组.结论:泡球蚴感染可引起小鼠脾细胞凋亡,而多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗可在一定程度上抑制感染鼠脾细胞的凋亡.     

致尿路感染大肠杆菌致病性的初步研究

用血凝试验对85例尿路感染患者尿液标本,79例其它患者脓汁、痰液等标本,以及36例正常粪便标本中大肠杆菌株的血凝特性进行测定。发现致尿路感染大肠杆菌株的甘露糖抵抗血凝(MRHA)反应阳性率为44.7%,明显高于正常人粪便标本(阳性率2.8%)相似文献