亚硒酸钠诱导白内障的机理研究

在pH8.0-8.5条件下,谷胱甘肽、氧或过氧化氢过量时,微量的10~(-4)mol·L~(-1)亚硒酸钠能使鲁米诺产生稳定的化学发光。发光强度依赖于酸度、谷胱甘肽浓度、氧及过氧化氢的浓度。超氧化物歧化酶(SOD)对鲁米诺发光有明显的抑制作用。SeO_3~(2-)在眼内催化产生活性氧可能是产生白内障的原因。     

松果体和褪黑激素通过下丘脑影响大鼠腹腔巨噬细胞化学发光(英文)

目的:研究松果体和褪黑激素(Mel)是否通过下丘脑影响腹腔巨噬细胞功能.方法:松果体切除术;腹腔巨噬细胞化学发光测定;下丘脑地诺前列酮放射免疫测定;下丘脑注射Mel.结果:松果体切除后腹腔巨噬细胞化学发光值降低,下丘脑地诺前列酮含量升高,16:00 ip Mel(10 μg kg~(-1)d~(-1)×7d)可使其恢复,并升高正常大鼠腹腔巨噬细胞化学发光值,降低其下丘脑地诺前列酮含量.腹腔巨噬细胞化学发光值与下丘脑地诺前列酮含量的变化存在负相关(相关系数,r=-0.78,P<0.01).于下丘脑注射Mel 2μg,能提高正常大鼠和松果体切除大鼠腹腔巨噬细胞化学发光值.结论:下丘脑是松果体Mel影响腹腔巨噬细胞功能的主要作用部位之一.     

生姜对超氧阴离子及羟自由基的清除作用

生姜对次黄嘌呤-黄嘌呤氧化酶体系产生的超氧阴离子及紫外线照射H₂O₂体系产生的羟自由基均有显著清除作用。提示生姜对活性氧的清除可能是其重要的药理机制之一。     

镍作业工人细胞免疫功能、血清硒和过氧化脂质含量的变化

９６名镍作业工人测定结果：非特异酯酶阳性细胞百分率（ＡＮＡＥ＋％）为７６．８％±８．０％，Ｔ细胞亚群ＣＤ２、ＣＤ４、ＣＤ８阳性细胞百分率（ＣＤ＋２％、ＣＤ＋４％、ＣＤ＋８％）及ＣＤ＋４／ＣＤ＋８比值分别为６５．６％±１０．５％、５６．３％±１２．１％、３４．３％±８．２％和１．７６±０．６；酵母多糖刺激的外周血多形核白细胞化学发光（ＰＭＮ－ＣＬ）本底和峰值分别为４８±２３和３０７３±６８４ＣＰＳ／１０６ＰＭＮ；血清硒和丙二醛含量分别为１．２２±０．２３和４．７６±０．８８μｍｏｌ／Ｌ。与非镍作业的地区对照组比较，Ｔ细胞ＣＤ＋８％增高，ＣＤ＋４／ＣＤ＋８比值下降，化学发光的本底值降低，峰值增加，血清Ｓｅ含量下降，丙二醛含量升高。分析镍作业工人工龄与后三项指标变化的关系，工龄大于２０年与小于１０年有统计学上明显差别。这些观察指标为镍作业人员医学观察增加新的监护指标提供依据。     

Additive effects of earthworm extract with HpD-laser on the enhancement of reactive oxygen production and the inhibition of[~3H]—thymidine.uptake in mouse ascitic tumor cells

The effects of combined use of earthworm extract(912)and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S_(180) tumor cells were studied throughchemiluminescence measurement and[~3H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[~3H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O_2~ and H_2O_2 in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.     

(一)表没食子儿茶素没食子酸酯对活性氧自由基的清除作用机制

本文研究了(一)表没食子儿茶素没食子 酸酯[(—)-EGCG]清除O_(?)和·OH的半数清除率SC_(50)、清除速率常数k和清除反应的化学计量因子n,用体外模型分析了药物对活性氧自由基的清除机制和(—)-EGCG自由基的启动及结构特点,推论药物的清除反应中心为B、D和A环,每分子药物可捕捉6个O_(?)或·OH,与化学计量因子n=6相符。     

地尔硫对冠心病的抗氧化作用

目的：观察地尔硫对冠心病病人的抗脂质过氧化及抗氧自由基作用。方法：冠心病病人３０例为治疗组（男性２１例，女性９例，年龄５８±ｓ６ａ），给予地尔硫３０ｍｇ，ｐｏ，ｔｉｄ，共４ｗｋ。健康人组３０例（男性１８例，女性１２例，年龄５８±９ａ）。结果：治疗组在给药前较健康人组的血清ｏｘＬＤＬ，ＬＰＯ，ＳＣＬ，ＬＣＬ值显著升高，Ｐ＜０．０１，ＧＳＨ－Ｐｘ显著降低，Ｐ＜０．０１，ＳＯＤ活力两者无明显差异，Ｐ＞０．０５。治疗组用药后与用药前相比ｏｘＬＤＬ，ＬＰＯ，ＳＣＬ，ＬＣＬ值显著下降，Ｐ＜０．０１或＜０．０５，ＧＳＨ－Ｐｘ显著升高，Ｐ＜０．０１，治疗组治疗后与健康人组相比，除ＬＣＬ外，其他指标皆无明显差异，Ｐ＞０．０５。结论：地尔硫是一良好的抗氧化剂。     

Priming effect of RANTES on eosinophil oxidative metabolism

Background RANTES has been shown to possess chemotactic activity for eosinophils, which have also been considered to play a role in allergic inflammation through reactive oxygen species. Thus, in this study, we examined the effect of RANTES on radical oxygen products from eosinophils.
Methods Purified eosinophils by CD16-negative selection or an eosinophilic cell line (EoL-1) were incubated with or without RANTES (2.5 x 10⁻⁶). To the mixture of eosinophils and luminol, calcium ionophore (A23187) or opsonized zymosan (OZ) was added, and radical oxygen products were determined by luminol-dependent chemiluminescence for 600 s.
Results Eosinophil-mediated radical oxygen products of untreated eosinophils produced with A23187 gave a peak value of 14.09 + 2.40 (mean±SE, n = 12) relative light units (RLU) and an integrated value of 3232.20 + 513.09 RLU. However, with treatment with RANTES, a peak value of 18.66 + 2.40 RLU and an integrated value of 5301.05 ±561.02 RLU were obtained. Eosinophil oxidative metabolism-induced A23187 or OZ was apparently augmented by the preincubation with RANTES. In addition, the radical oxygen products of EoL-1 showed similar results.
Conclusions Thus, we concluded that RANTES may play an important role the pathogenesis of allergic inflammation through its involvement in eosinophil activation, as evidenced by oxygen products, as well as in selective eosinophil infiltration as selective eosinophil chemoattractant.     

C3b-Mediated chemiluminescence of human lymphocytes

Department of Microbiology, S. M. Kirov Medical Institute, Gor'kii. (Presented by Academician of the Academy of Medical Sciences of the USSR Yu. A. Vladimirov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 108, No. 11, pp. 587–589, November, 1989.