清燥救肺汤加减治疗依那普利所致咳嗽临床研究

目的 :观察清燥救肺汤加减治疗依那普利所致咳嗽疗效。方法 :设清燥救肺汤组与复方甘草片组对照观察疗效及对血压的影响。结果 :与对照组比较 ,疗效有显著差异 ,P <0 .0 1;血压有明显改善 ,P <0 .0 5。结论 :清燥救肺汤用于治疗依那普利所致咳嗽疗效肯定。     

胃久灵抗溃疡、抗胃炎作用及毒性的初步研究

以中药验方胃久灵对大白鼠的两种急性溃疡模型、一种慢性溃疡模型及两种胃炎模型用药治疗,证明胃久灵确有抗溃疡抗胃炎作用.其中对慢性溃疡的治疗作用较为突出.经急、慢性毒性实验,未见对重要脏器有任何毒性损害.     

基于系统药理学对山茱萸“救脱”原理的物质基础研究

目的：根据经典中医文献及现代研究探索山茱萸发挥"救脱"功能的原理，发掘潜在的治疗"脱证"等疾病的未被开发的活性化合物。方法：利用中药系统药理学数据库与分析平台（TCMSP）获取山茱萸的化学成分，并根据Lipinski规则（LR）、口服生物利用度（Oral bioavailability，OB）以及药物相似性（Drug-likeness，DL）对潜在的生物活性成分进行分析鉴定；利用BATMAN-TCM对获得的活性成分进行生物信息学分析。结果：从山茱萸中获得226种化学成分，并根据LR、OB及DL特性筛选出潜在有效成分19个。并分析这些有效成分相作用的靶点，构建h-c-t-d网络关系。结论：发现山茱萸可能是通过硬脂酸、亚麻酸、亚麻油酸等化合物，影响ALOX5AP、BDKRB2、PTGS2、SCN5A等靶点，通过提高血压，强心，镇痛的协同作用，达到救脱的目的。     

《素问纠略》作者、版本考

《素问纠略》一书的作者及版本历来说法不一。《明史》记为杨慎,但考查杨慎文集《升庵全集》《升庵外集》均不见此书;丹波元胤《中国医籍考》认为此书应是朱丹溪所作。本文就此提出疑问,考查杨慎著述的主客观条件。首先,从史传及杨慎诗文中可知,他曾研习医籍,知晓医理,其医学著作《男女脉位图说》序文更反映了他欲纠辨古人的愿望,是为他著《素问纠略》的主观条件或内部条件;其次,杨慎晚年贬谪至西南地区,恶病缠身,曾学医自疗,且喜好上山采药,这为他创作《素问纠略》提供了外部条件;加之同僚何宇度、名医何时希的记载为旁证,杨慎著《素问纠略》三卷是成立的。而针对杨慎文集不见此书,一卷本著者题为朱丹溪、周木等人的事实,本文认为是版本不一所致。即:名为《素问纠略》的医书实有两个版本:其一为前述杨慎所著的三卷本,已经亡佚;其二为周木辑佚或假托元代朱震亨所作的《素问纠略》一卷本,现存抄本。由此,《素问纠略》作者及版本问题得以澄清。     

崔应珉教授治疗肝癌学术思想探讨

崔应珉教授认为,肝癌的病理基础为脾虚气滞、痰瘀毒结,而脾气亏虚是癌变发生的关键.治疗中从脾论治,以健脾益气为主,结合理气化湿等多种治法,创健脾益气方,临证辨证加减施治,同时结合调畅情志、饮食摄生等配合治疗,在肝癌的治疗中获得了较好的疗效.     

A REPORT OF THE TREATMENT OF 103 CASES WITH POSTPARTUM RETENTION OF URINE

The acupuncture treatment with Zi Wu Dao Jiu needling was used to treat thepostpartum retention of urine and produced a therapeutic effect better than that of urethral catheteri-zation. The remarkable effectiveness rate in patients with a clinical course less than 4 days was 99％.The acupuncture at Huiyang (BL 35) point might stimulate pudendal nerve and relax muscles of uri-nary bladder and urethra to facilitate the discharge of urine.     

消痛救心散联合消心痛治疗冠心病心绞痛50例疗效观察

目的观察消痛救心散治疗冠心病心绞痛的临床效果。方法80例冠心病心绞痛患者随机分为治疗者50例和对照组30例,治疗组应用消痛救心散联合消心痛治疗,对照组单用消心痛治疗,观察2组疗效。结果治疗组心绞痛治疗有效45例（90．0％）,对照组有效19例（63．3％）。2组比较差异有统计学意义（P〈0．01）。治疗组心电图疗效有效33例（66．0％）,对照组有效10例（33．3％）。2组比较差异亦有统计学意义（P〈0．05）。结论消痛救心散治疗冠心病心绞痛效果满意,值得临床推广应用。     

自拟九味茶治疗代谢综合征临床观察

目的观察自拟九味茶治疗代谢综合征的临床疗效。方法将60例代谢综合征患者随机分为治疗组和对照组（各30例）,治疗组予自拟九味茶治疗,对照组予卡托普利片治疗,疗程均为3个月。观察两组治疗前后体质量指数、血压、空腹血糖（FBG）、血胆固醇（TG）、三酰甘油（TC）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）及中医证候积分等变化。结果治疗组治疗后体质量指数、收缩压、舒张压均较治疗前降低（P〈0.01）,而对照组治疗后仅收缩压降低（P〈0.05）。治疗组体质量指数下降幅度大于对照组（P〈0.01）。治疗组治疗后总胆固醇、三酰甘油较治疗前降低（P〈0.01）,且三酰甘油低于对照组（P〈0.05）。治疗组治疗后头晕、乏力、胸闷、心悸、口干、头痛等积分及总积分减少（P〈0.01）,且除失眠外,两组各项指标积分及总积分均低于对照组（P〈0.05,P〈0.01）。结论自拟九味茶具有改善代谢综合征的作用。     

Identification of metabolites of crude and processed Fructus Corni in rats by microdialysis sampling coupled with electrospray ionization linear quadrupole ion trap mass spectrometry

A microdialysis (MD) sampling coupled with electrospray ionization linear quadrupole ion trap mass spectrometry (LTQ-MSⁿ) method has been developed for rapid and sensitive analysis of rat microdialysate metabolite profile of Fructus Corni, a well-known Traditional Chinese Medicine (TCM). The purified samples were separated by a reversed-phase HPLC with C₁₈ column under a gradient elution. Parent compounds and metabolites of crude and processed Fructus Corni of Jiu Zheng Pin (JZP, JZP is produced after steaming the crude drug pre-steeped in wine) were detected by the on-line MSⁿ detector in negative scan model. The identification of the metabolites and their structural elucidation were performed by comparing the changes in molecular mass and defining sites of biotransformation based on the accurate MSⁿ spectral information of diagnostic fragment ions. In this work, we used such strategies for the identification of the parent compounds and metabolites of crude and processed Fructus Corni in rats, and seven parent compounds and three new metabolites of Fructus Corni were found in rats for the first time. This study provides important structural information regarding to the metabolism of crude Fructus Corni and its JZP. Furthermore, this work also demonstrated the possibilities of using microdialysis sampling coupled with LC-MSⁿ approach for identification of bioactive compounds from TCM in vivo.     

Effect of Tong Luo Jiu Nao on Aβ-degrading enzymes in AD rat brains

Ethnopharmacological relevance

Tong Luo Jiu Nao (TLJN) is a modern Chinese formula based on Traditional Chinese Medicine theory that has been used to treat ischemic cerebral stroke and vascular dementia. TLJN belongs to the ethnopharmacological family of medicines. In this study, we investigated the mechanism of the TLJN effect on Alzheimer's disease (AD).

Aim of the study

To investigate the effect of TLJN on β-amyloid-degrading enzymes and learning and memory in the AD rat brain.

Materials and methods

AD rats whose disease was induced by Aβ_25-35 injection into the bilateral hippocampus CA1 region were subjected to intragastric administration of various preparations. The experimental animals were healthy male Sprague-Dawley rats which were randomly divided into normal, sham, model, TLJN min, TLJN max and donepezil hydrochloride groups. Spontaneous alternation and passive avoidance behavior, which are regarded as measures of spatial learning and memory, were investigated using Y-maze testing. Western blotting and immunohistochemistry were used to observe the therapeutic effect of TLJN on the deposits of amyloid plaque and on the expression of synaptophysin, insulin-degrading enzyme and neprilysin.

Results

Y-maze results showed that the AD model group presented with spatial learning and memory impairments. Hematoxylin-eosin and Congo red staining indicated neuronal impairment and deposits of amyloid plaque in the model group and these results were consistent with their learning and memory deficits in the Y-maze. The TLJN-treated groups exhibited prolonged a cavity delitescence, decreased arm entries and improvement in learning and memory. Moreover, the structure of the neurons of the treated groups was restored and the expression of synaptophysin increased in both the hippocampus and cortex. In addition, their levels of insulin-degrading enzyme and neprilysin in the cortex and hippocampus were upregulated and the amyloid plaque was decreased.

Conclusion

TLJN can improve learning and memory, up-regulate insulin-degrading enzyme and neprilysin levels, promote the degrading of Aβ and clear amyloid plaque from the AD rat brain. In future, TLJN may have significant therapeutic potential in the treatment of AD patients.