高效液相色谱法测定好望角芦荟中芦荟苷的含量

目的：建立高效液相色谱法测定好望角芦荟中芦荟苷的含量。方法：流动相为乙腈-水（25：75），检测波长355nm，样品提取过滤后进行分析。结果：线性范围为0．4008～4．008μg·μL^-1，相关系数为r=0．9997，精密度为0．7％，加样回收率为99．7％。好望角芦荟中芦荟苷的含量为20．1％。结论：该法简便、灵敏，结果可靠准确，适用于芦荟中芦荟苷的含量测定。     

芦荟苷预处理对缺血再灌注大鼠心肌细胞凋亡的影响

目的研究芦荟苷(Barb)对急性心肌缺血再灌注损伤大鼠心肌细胞凋亡的影响。方法结扎大鼠左冠状动脉前降支复制心肌缺血再灌注损伤模型,实验分为假手术组,缺血再灌注组(IR),Barb高、低剂量预处理组。采用原位缺口末端标记法(TUNEL)检测心肌细胞凋亡指数(AI),酶联免疫吸附试验(ELISA)法测定心肌细胞肿瘤坏死因子(TNF)-α水平,化学法测定线粒体Ca2+-ATPase活性。结果与IR组比较,Barb组AI和TNF-α水平显著降低(P<0.01或P<0.05),Ca2+-ATPase活性明显增高(P<0.05)。结论Barb能明显抑制IR引起的心肌细胞凋亡,其作用可能与降低TNF-α水平和提高Ca2+-ATPase活性有关。     

芦荟苷预处理对缺血再灌注大鼠心肌细胞凋亡、TNF-α含量和Ca2+-ATPase活性的影响

摘要：目的研究芦荟苷（Barbaloin,Barb）对急性心肌缺血再灌注损伤大鼠心肌细胞凋亡的影响。方法结扎大鼠左冠状动脉前降支复制心肌缺血再灌注损伤模型,实验分为假手术组、缺血再灌注组（IR）、Barb高、低剂量预处理组。采用原位缺口末端标记法（TUNEL）检测心肌细胞凋亡指数（AI）,ELISA法测定血清TNF—α水平,化学法测定线粒体Ca^2＋-ATPase活性。结果与IR组比较,Barb组AI和TNF-α水平显著降低（P〈0．05或P〈0．01）,Ca^2＋-ATPase活性明显增高（P〈0．05）。结论Barb能明显抑制IR引起的心肌细胞凋亡,其作用可能与降低TNF-α水平和提高Ca2＋-ATPase活性有关。     

对药品中芦荟苷含量测定的改进建议

目的：建立了一种同时测定金嗓子喉片中没食子酸和绿原酸含量的二极管阵列检测器色谱（HPLC-PDA）方法。方法：采用资生堂C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.1%磷酸水为流动相进行梯度洗脱,流速为1.0 mL·min-1,柱温为25 ℃,检测波长设为270 nm（没食子酸）和330 nm（绿原酸）。结果：没食子酸与绿原酸浓度分别在10.02~80.16μg·mL-1（r=0.999 8）和5.973~47.78 μg·mL-1（r=0.999 7）范围内与峰面积呈良好的线性关系,平均回收率分别为100.3%（RSD=0.59%）和99.6%（RSD=0.85%）,待测液在室温的条件下,60 h之内保持稳定。结论：本方法专属性强,重复性好,结果准确,可用于金嗓子喉片中没食子酸、绿原酸的含量测定。     

Selective determination of aloin in different matrices by HPTLC densitometry in fluorescence mode

A novel method based on the fluorescence excited solely on aloin by a H₃BO₃ derivatizing procedure, allowed its rapid and selective determination among the co-occurring components in a variety of complex matrices as several Aloes dried extracts and related commercial products.     

高效液相色谱法测定芦荟中芦荟苷的含量

本文建立了芦荟中芦荟苷含量测定的高效液相色谱方法 ,考察了芦荟中芦荟苷含量与其产地的关系。色谱柱 :HypersilODS柱 (4 6mm× 2 0 0mm ,5 μm) ;流动相 :甲醇 水 (4 0 :6 0 ,含有 0 1%冰醋酸 ) ;流速 :1 0mL·min- 1 ;检测波长 :35 9nm ;柱温 :30℃。结果表明 :在 0 0 72 6 - 0 72 6 μg范围内芦荟苷与峰面积呈良好线性关系 ,相关系数为 0 9998,回归方程为Y =1 92 0 2× 10 6X - 180 1 9。芦荟苷在甲醇溶液中 48小时内稳定 ,仪器精密度为 1 2 % ,方法精密度为 4 9%。 12个不同产地的芦荟样品中 ,芦荟苷含量在 6 16 0 - 319 1μg·g- 1 范围内。本方法快速简便 ,重现性好 ,芦荟苷含量由于生态环境的影响而差异显著     

芦荟甙的伏安行为及其应用研究

芦荟甙在0.1mol·L^-1 H₂SO₄底液中,出现一灵敏的示波极谱导数峰,峰电位为-0.75V(vsSCE),峰电流与芦荟甙浓度在2.0×10^-7～6.0×10^-6mol·L^-1范围内成线性关系,用于芦荟原胶中芦荟甙的测定。用线性扫描和循环伏安法等手段研究体系的伏安行为,特别是吸附性。实验表明,该体系属具有吸附性的可逆过程。     

Barbaloin: A concise report of its pharmacological and analytical aspects

Barbaloin is C-glucoside of aloe emodin anthrone which is found in the plant name as Aloe vera is a perennial succulent (Liliaceal), also called the healing plant. Barbaloin have variety of pharmacological activity such as strong inhibitory effect on histamine release, anti-inflammatory, cathartic, antiviral, antimicrobial, anticancer, antioxidant activity and alternative for pharmaceutical or cosmetic applications. The peak amount of barbaloin was reached about 3 h after oral administration. Concentration of barbaloin in Aloe vera leaves was shown to depend on the leaf part, age, and position of the leaf. Young leaves contain more barbaloin compared to old one. Various researches have been done on barbaloin but still the relationship between the barbaloin and its overall effect has not been clarified. A more specific perceptive of the pharmacological activities of barbaloin is required to develop for pharmaceutical purpose. Many attempts have been made regarding its isolation, biological activity to examine their effects, and clarify their functional mechanism. This review gives a brief idea about its uses, ethnomedicinally and commercially important analytical techniques and their pharmacological activities.     

HPLC法测定芦荟保健制品中芦荟苷的含量

目的:建立芦荟保健制品中芦荟苷含量的测定方法。方法:高效液相色谱法,以Hypersil-C18(4.6 mm×150 mm,5μm)为色谱柱,甲醇-水(55:45)为流动相,检测波长为356 nm,流速1.0 ml/min,柱温25℃。结果:芦荟苷在0.01~0.1 mg/ml范围内与峰面积呈良好的线性关系,r=0.9992,芦荟苷平均加标回收率为97.3%,RSD为1.13%(n=9)。结论:试验结果表明本方法可靠性高,准确性和重现性好,适用于芦荟保健制品中芦荟苷的质量控制。     

高效液相色谱法测定维吾尔药则海甫片中芦荟苷的含量

目的:建立以高效液相色谱法测定维吾尔药则海甫片中芦荟苷含量的方法。方法:色谱柱为ShimadzuODSVP,流动相为甲醇-0.4%磷酸水溶液(43∶57),检测波长为360nm。结果:芦荟苷在12.4~74.4μg.mL-1浓度范围内呈良好的线性关系(r=0.9999),平均回收率为99.93%,(RSD=1.23%,n=9)。结论:本方法简便、快速、准确、重现性好,能控制该制剂的质量。