Rheb1在小鼠巨核-红系多能性干细胞发育中的作用

目的:研究Rheb1基因在小鼠巨核-红系多能性干细胞发育成熟中的作用及相关的机制。方法:利用Vav-Cre在造血系统中特异性敲除小鼠Rheb1(Vav1-Cre;Rheb1^fl/fl,Rheb1^Δ/Δ小鼠),采用流式细胞术检测Rheb1敲除组和对照组小鼠骨髓和外周血中红系细胞的比例;CFC克隆形成实验检测敲除组和对照组小鼠骨髓中巨核-红系多能性干细胞体外克隆形成能力;利用实时荧光定量PCR检测敲除组和对照组小鼠巨核-红系多能性干细胞中PU.1、GATA-1、GATA-2、CEBPα和CEBPβ的相对表达量;在培养基中加入雷帕霉素,检测野生型小鼠巨核-红系多能性干细胞体外克隆形成能力的变化。结果:Rheb1敲除后,小鼠骨髓中红系细胞发育受抑且应激能力减弱,小鼠骨髓中巨核-红系多能性干细胞体外克隆形成能力减弱,GATA-1的表达水平降低;雷帕霉素可以抑制野生型小鼠骨髓中巨核-红系多能性干细胞体外克隆的形成。结论:Rheb1基因在小鼠巨核-红系多能性干细胞发育中具有重要的调控作用,Rheb1可能通过mTOR信号通路调控小鼠巨核-红系多能性干细胞发育...     

前臂游离皮瓣修复口腔颌面部缺损84例

目的：总结提高前臂桡侧游离皮瓣成活率的经验。方法：选择血供良好的左前臂桡侧皮瓣,先从设计皮瓣远端内侧及外侧1／3边缘切开皮瓣的切取和显微外科的血管吻合方法,术后严密观察皮瓣的色泽变化。结果：84例皮瓣修复中,78例完全成活,成活率93％;6例坏死。结论：前臂皮瓣的切取及血管吻合方法的改进可使皮瓣保持血流平衡,提高游离皮瓣的成活率。     

从结构相似模型化合物进行天然产物结构鉴定-以番石榴叶黄酮苷类化合物鉴定为例

天然产物的结构鉴定主流手段是核磁共振波谱法,与结构相似模型化合物的核磁数据进行比较,确定未知化合物结构是一种重要的方式。论文从结构鉴定过程中有机分子可分拆解析,如何寻找相似模型化合物和实例例举3个方面,探讨相似模型化合物对于未知图谱结构鉴定的作用和意义,并建议在平时的结构分析过程中注重模型化合物数据的收集和积累。     

周围腺体组织瓣在乳腺癌保乳术中局部缺损修复中的应用

目的：研究周围腺体组织瓣在乳腺癌保乳术中局部缺损修复中的应用效果。方法选取行乳腺癌保乳术患者72例,分为A组和B组,各36例,A组进行皮肤直接缝合,B组进行周围腺体组织瓣转位整形,比较2组美容效果以及术后并发症情况。结果 A组进行皮肤直接缝合,不满意8例,不满意率为22.22%,显著高于于B组行周围腺体组织瓣转位整形的1例及2.78%,差异具有统计学意义（χ2=4.57,P<0.05）;B组进行周围腺体组织瓣转位整形方法美容效果优于A组进行皮肤直接缝合（χ2=4.57,P<0.05）。结论周围腺体组织瓣在乳腺癌保乳术中局部缺损修复中的应用效果良好,患者满意度高,具有更好的美容效果。     

冠状动脉慢血流现象临床危险因素及形态学研究

目的探寻冠状动脉慢血流现象(CSFP)的临床危险因素,了解冠状动脉形态对心肌梗死溶栓治疗试验(TIMI)血流的影响,推测CSFP的发病机制。方法回顾性收集2017年1月至2019年7月于兰州大学第一医院心脏中心行冠状动脉造影无狭窄病变的患者256例,根据TIMI血流帧数将其中血流减慢的患者设为CSFP组(100例),将血流正常患者设为对照组(156例),按年龄和性别匹配。收集患者临床数据和冠状动脉造影数据,采用二元logistic回归模型分析CSFP的危险因素,并采用Pearson相关分析检验冠状动脉形态学参数与平均校正的TIMI帧数(cTFC)的相关性。结果CSFP组同型半胱氨酸[(22.07±7.27)mmol/L比(18.16±6.63)mmol/L]、中性粒细胞百分比[(44.40±15.60)%比(35.43±12.49)%]、淋巴细胞百分比[3.65%(1.32%,24.65%)比1.80%(1.02%,6.22%)]、平均血小板体积(MPV)[(13.17±1.82)fl比(12.20±1.57)fl]、血小板分布宽度(PDW)[(14.17±2.17)f l比(13.21±2.42)f l]与对照组相比更高,三酰甘油[(3.05±1.37)mmol/L比(3.44±1.36)mmol/L]、纤维蛋白原[(2.65±0.68)g/L比(2.86±0.66)g/L]与对照组相比更低,术中血压更低,冠状动脉平均直径更大,平均远端分支数更少,差异均有统计学意义(均P<0.05)。二元logistic回归模型拟合显示:同型半胱氨酸(OR 1.077,95%CI 1.034~1.123,P<0.001)、中性粒细胞百分比(OR 1.064,95%CI 1.032~1.097,P<0.001)、PDW(OR 1.194,95%CI 1.051~1.356,P=0.006)、MPV(OR 1.338,95%CI 1.127~1.588,P=0.001)是预测CSFP的独立危险因素。相关性分析显示,冠状动脉平均直径与平均cTFC呈显著正相关(r=0.583,P<0.05);冠状动脉平均远端分支数与平均cTFC呈显著负相关(r=-0.508,P<0.05)。结论同型半胱氨酸、中性粒细胞百分比、PDW和MPV是预测CSPF的独立危险因素。冠状动脉平均直径和远端分支数均与冠状动脉平均cTFC密切相关。炎症可能是CSFP发生的关键机制。     

HPLC-MS3分析心叶淫羊藿有效部位的化学成分

目的：研究心叶淫羊藿Epimedium brevicornum有效部位中的化学成分。方法：乙醇提取,大孔树脂柱纯化得有效部位；运用HPLC-MS³联用技术分析化合物结构。结果：从心叶淫羊藿有效部位中鉴定出9个化合物。结论：HPLC-MS³能简单、快速地分析心叶淫羊藿黄酮苷类化学成分。     

Antimalarial activity of Bidens pilosa L. (Asteraceae) ethanol extracts from wild plants collected in various localities or plants cultivated in humus soil

Bidens pilosa (Asteraceae), a medicinal plant used worldwide, has antimalarial activity as shown in previous work. This study tested ethanol extracts from wild plants collected in three different regions of Brazil and from plants cultivated in various soil conditions. The extracts were active in mice infected with P. berghei: doses of < or =500 mg/kg administered by oral route reduced malaria parasitaemia and mouse mortality; higher doses were found to be less effective. Tested in vitro against three P. falciparum isolates, two chloroquine resistant and one mefloquine resistant, the plants cultivated under standard conditions, and in humus enriched soil, were active; but the wild plants were the most active. Analysis using thin layer chromatography demonstrated the presence of flavonoids (compounds considered responsible for the antimalarial activity) in all plants tested, even though at different profiles. Because B. pilosa is proven to be active against P. falciparum drug-resistant parasites in vitro, and in rodent malaria in vivo, it is a good candidate for pre-clinical tests as a phytotherapeutic agent or for chemical isolation of the active compounds with the aim of finding new antimalarial drugs.     

Evaluation of the antiherpetic activity of standardized extracts of Achyrocline satureioides

Traditionally, Achyrocline satureioides or 'marcela' has been used in South America for the treatment of several disorders. For the present study, three spray-dried extracts (N1, N2 and N3) were used, all of them prepared with 50% of an hydroethanolic extract rich in flavonoid compounds and 50% of blends of different adjuvants. The cytotoxic concentration which causes destruction in 50% monolayer cells (CC50) was 62.5 microg/ml for the three extracts. The antiviral activity was evaluated by using two different strains of herpes simplex virus (HSV-1) and the best results were obtained with KOS strain and N2 extract. Studies concerning the mechanism of the antiherpetic activity demonstrated that N2 extracts showed no virucidal effect or activity on cellular receptors. HSV-1 DNA synthesis was not inhibited. The antiherpetic activity occurred between the second and ninth hour of the virus replication cycle, probably indicating a perturbation on late stages of this cycle.     

Trypanocidal activity of chemical constituents from Lychnophora salicifolia Mart

The ethyl acetate extract from leaves plus inflorescences of L. salicifolia showed significant trypanocidal activity against trypomastigote forms of T. cruzi, which was due to the flavonoid quercetin-7,3',4'-trimethyl ether and the sesquiterpenoid lychnopholic acid. Despite the use of some species of Lychnophora in folk medicine for pain relief, crude extracts from L. salicifolia Mart. showed no antinociceptive activity in the mouse writhing test.