全文获取类型
收费全文 | 3002篇 |
免费 | 266篇 |
国内免费 | 68篇 |
专业分类
耳鼻咽喉 | 21篇 |
儿科学 | 51篇 |
妇产科学 | 50篇 |
基础医学 | 352篇 |
口腔科学 | 79篇 |
临床医学 | 381篇 |
内科学 | 294篇 |
皮肤病学 | 105篇 |
神经病学 | 179篇 |
特种医学 | 110篇 |
外科学 | 114篇 |
综合类 | 521篇 |
预防医学 | 220篇 |
眼科学 | 32篇 |
药学 | 453篇 |
1篇 | |
中国医学 | 127篇 |
肿瘤学 | 246篇 |
出版年
2024年 | 4篇 |
2023年 | 35篇 |
2022年 | 48篇 |
2021年 | 91篇 |
2020年 | 77篇 |
2019年 | 74篇 |
2018年 | 100篇 |
2017年 | 111篇 |
2016年 | 106篇 |
2015年 | 119篇 |
2014年 | 218篇 |
2013年 | 250篇 |
2012年 | 219篇 |
2011年 | 276篇 |
2010年 | 186篇 |
2009年 | 184篇 |
2008年 | 173篇 |
2007年 | 163篇 |
2006年 | 122篇 |
2005年 | 111篇 |
2004年 | 93篇 |
2003年 | 78篇 |
2002年 | 65篇 |
2001年 | 56篇 |
2000年 | 45篇 |
1999年 | 39篇 |
1998年 | 38篇 |
1997年 | 23篇 |
1996年 | 28篇 |
1995年 | 25篇 |
1994年 | 20篇 |
1993年 | 16篇 |
1992年 | 14篇 |
1991年 | 13篇 |
1990年 | 13篇 |
1989年 | 8篇 |
1988年 | 10篇 |
1987年 | 19篇 |
1986年 | 7篇 |
1985年 | 16篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1982年 | 10篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有3336条查询结果,搜索用时 46 毫秒
1.
Sebastian Mondaca Walid K. Chatila David Bates Jaclyn F. Hechtman Andrea Cercek Neil H. Segal Zsofia K. Stadler Anna M. Varghese Ritika Kundra Marinela Capanu Jinru Shia Nikolaus Schultz Leonard Saltz Rona Yaeger 《Clinical colorectal cancer》2019,18(1):e39-e52
Background
Treatment of advanced anal squamous cell cancer (SCC) is usually with the combination of cisplatin and 5-fluorouracil, which is associated with heterogeneous responses across patients and significant toxicity. We examined the safety and efficacy of a modified schedule, FOLFCIS (leucovorin, fluorouracil, and cisplatin), and performed an integrated clinical and genomic analysis of anal SCC.Patients and Methods
We reviewed all patients with advanced anal SCC receiving first-line FOLFCIS chemotherapy – essentially a FOLFOX (leucovorin, fluorouracil, and oxaliplatin) schedule with cisplatin substituted for oxaliplatin – in our institution between 2007 and 2017, and performed deep sequencing to identify genomic markers of response and key genomic drivers.Results
Fifty-three patients with advanced anal SCC (48 metastatic; 5 unresectable, locally advanced) received first-line FOLFCIS during this period; all were platinum-naive. The response rate was 48% (95% confidence interval [CI], 32.6%-63%). With a median follow-up of 41.6 months, progression-free survival and overall survival were 7.1 months (95% CI, 4.4-8.6 months) and 22.1 months (95% CI, 16.9-28.1 months), respectively. Among all patients with advanced anal SCC that underwent sequencing during the study period, the most frequent genomic alterations consisted of chromosome 3q amplification (51%) and mutations in PIK3CA (29%) and KMT2D (22%). No genomic alteration correlated with response to platinum-containing treatment. Although there were few cases, patients with human papillomavirus-negative anal SCC did not appear to benefit from FOLFCIS, and all harbored distinct genomic profiles with TP53, TERT promoter, and CDKN2A mutations.Conclusions
FOLFCIS appears effective and safe as first-line chemotherapy in patients with advanced anal SCC and represents an alternative treatment option for these patients. 相似文献2.
Kayoko Minakata Masako Suzuki Hideki Nozawa Kunio Gonmori Kanako Watanabe Osamu Suzuki 《Forensic Toxicology》2006,24(2):83-87
Platinum (Pt) levels were determined in various tissues and body fluids obtained from a patient who died 181 days after cisplatin
overdosing. The symptoms of cisplatin overdose, however, might have almost disappeared by day 40, and the patient’s death
was ascribed to the recurrence of malignant lymphoma. Determination of Pt derived from cisplatin was performed by electrospray
ionization mass spectrometry (ESI-MS) using silver (Ag) as internal standard. Pt and Ag complexed with diethyldithiocarbamate
(DDC) in wetashed blood, and tissue solutions were extracted into isoamyl alcohol, and then acidified with oxalic acid. By
injecting an aliquot of the isoamyl alcohol layer into a mass spectrometer in the direct flow injection mode, the quantitation
was performed using the signals of Pt(DDC)3
+ and Ag(DDC)2
+ at m/z 639 and 403, respectively. The Pt levels ranged from 25ng/ml in blood to 2050ng/g wet weight in the liver of the patient,
indicating that Pt remained at high levels in tissues, even after a period as long as 181 days after cisplatin overdosing. 相似文献
3.
BACKGROUND/PURPOSE: Sensitive skin is a condition associated with reduced tolerance to environmental factors and/or the application of topical products, such as cosmetics. Its pathophysiology has not been fully elucidated and few data are available on its prevalence. The aim of this study was to investigate possible correlation between objective sensitivity and skin surface microrelief. METHODS: During an epidemiological survey conducted for a campaign promoted by International Society of Plastic Dermatology in Italy, 243 adult healthy subjects of both sexes with no evident dermatological disorder but positive to the lactic acid stinging test, were submitted to cyanoacrylate stratum corneum stripping from the volar forearm for the determination of the irregularity of the skin surface microrelief (irregularity skin index (ISI)). RESULTS: A significant correlation was found between intensity of symptoms in stingers and ISI (r(s)=-0.47; P<0.001). CONCLUSION: Sensitive skin is common in the healthy population. ISI can contribute towards the identification of subjects with sensitive skin and the development of more specific skin treatments for this prevalent condition. 相似文献
4.
[目的]研究超敏C反应蛋白(hs-CRP)、总抗氧化状态(TAS)联合血脂检测在早老性痴呆症诊断中的应用价值:[方法]选择浦东新区精神卫生中心早老性痴呆专科门诊患者54例,作超敏C反应蛋白、总抗氧化状态与血脂检测。[结果]与对照组比较,实验组hs-CRP、TAS差异非常显著,t1=4.55,t2=2.79,P1〈0.001,P2〈0.01;血脂中甘油三酯、低密度脂蛋白胆固醇、载脂蛋白B、Lp(a)差异显著;t1=3.01,P1〈0.01,t2=2.21,P2〈0.05,t3=2.64,P3〈0.01,t4=1.91,P4〈0.05。[结论]超敏C反应蛋白、总抗氧化状态联合血脂(甘油三酯、低密度脂蛋白胆固醇、载脂蛋白B、Lp(a))检测对实验室诊断早老性痴呆症具有较好敏感性和特异性,临床应用前景乐观。 相似文献
5.
将临床细菌培养及其药物敏感试验结果定期进行统计、分析、反馈,既是医院分级管理和医院感染管理规范的有关内容和要求,更为临床诊断、治疗提供可靠的科学依据。 相似文献
6.
103例角膜溃疡的病因和病原及药物敏感试验的统计分析 总被引:7,自引:0,他引:7
对1995年1—12月间我院门诊103例角膜溃疡的病因、病原和药物敏感试验进行统计分析。病因:外伤59例(57.28%),继发感染34例(33.01%),原因不明8例(7.77%),戴接触镜2例(1.94%)。病原:真菌35例(33.98%),绿脓杆菌18例(17.48%),金黄色葡萄球菌15例(14.56%),表皮葡萄球菌9例(8.74%),淋球菌2例(1.94%)。对19例真菌性角膜溃疡同时进行细菌培养,有7例为混合感染,占36.84%。对几种主要检出菌的药物敏感试验表明:它们对普通抗菌素均可产生一定的耐药性 相似文献
7.
A rehabilitation program including foot sensory stimulation, balance and gait training with limited vision was performed in 24 patients with clinically defined sensory ataxia. There were 15 patients with bilateral somatosensory loss related to chronic neuropathy and nine patients with unilateral loss-related to multiple sclerosis. After training, balance control assessed using the Berg Balance Test improved similarly in both groups, and Romberg's sign disappeared in some patients, suggesting an improvement in dynamic balance and in the proprioceptive contribution. Conversely, balance assessed on a static force platform remained similar in the open-eyes condition and improved in the closed-eyes condition only in patients with unilateral sensory loss. These results show that ataxic patients can improve their balance with better results in dynamic conditions and that the relative contribution of proprioceptive and visual inputs may depend on the extent of somatosensory loss. 相似文献
8.
目的:探讨钙激活钾通道(KCa)、延迟整流型钾通道(Kdr)和ATP敏感型钾通道(KATP)在哮喘豚鼠气道高反应中的作用。 方法: 采用离体气管环张力实验,观察加入特异性钾通道阻断剂后,与不加阻断剂相比气管环对组胺反应的量效曲线的差异。 结果: (1)加入KCa阻断剂TEA后,对照组气管环对组胺的反应变化不显著,而哮喘组气管环对10-4mol/L、10-3mol/L组胺的收缩反应均显著低于不加TEA的气管环(P<0.01),量效曲线明显下移;(2)加入Kdr阻断剂4-AP后,对照组气管环对10-3mol/L组胺的最大收缩反应明显下降(P<0.05),组胺的量效曲线下移,哮喘组气管环对10-4mol/L、10-3mol/L组胺的收缩反应均低于不加4-AP的气管环(P<0.01),且下降程度较对照组大(P<0.05),量效曲线明显下移;(3)加入KATP阻断剂glibenclamide后,对照组和哮喘组气管环对组胺的量效曲线与不加glibenclamide的气管环相比变化均无明显差异。 结论: 在豚鼠哮喘模型中,KCa和Kdr活性的降低在气道高反应的发生中起介导作用,KATP作用不明显。 相似文献
9.
目的利用抑制消减杂交(suppressionsubtractivehybridization,SSH)和cDNA表达谱芯片筛选早期活化的肝星状细胞(hepaticstellatecell,HSC)和大鼠正常肝脏组织、轻度肝纤维化组织、重度肝纤维化组织中差异表达的基因。方法从SSH构建的大鼠轻度肝纤维化、重度肝纤维化2个差异cDNA文库中挑选1000条上调显著的基因,与正常大鼠4136条基因克隆制作成一张芯片,筛选早期活化的肝星状细胞相关基因。结果获得与HSC早期活化相关的上调基因有202条,下调基因有80条,其中血清和糖皮质激素调节蛋白激酶(serumandglucocorticoidsensitivekinase,SGK)在正常大鼠基因克隆和2周及8周SSH上调差异基因中均呈上调信号。结论联合应用SSH和cDNA表达谱芯片是筛选和鉴定不同样本中差异表达基因的快速、经济和有效的方法;SGK可能作为多种细胞信号传导通路和细胞磷酸化级联反应的一个功能性交汇点,参与了肝星状细胞的早期活化和信号传导。 相似文献
10.
超敏C反应蛋白及血脂水平与急性冠状动脉综合征患者6个月内预后的关系 总被引:3,自引:2,他引:1
目的:探讨超敏C-反应蛋白(hs-CRP)、血脂水平与急性冠状动脉综合征(ACS)患者6个月内预后的关系。方法:分别测定201例非ST段抬高的ACS患者入院时的hs-CRP和血脂水平,随访6个月,终点为冠状动脉事件(冠心病性死亡、非致死性急性心肌梗死、急诊再血管化、因不稳定型心绞痛引起的再住院)。hs-CRP≥3mg/L、总胆固醇(TC)/高密度脂蛋白胆固醇(HDL-C)≥4.7mmol/L为异常。结果:201例患者中发生冠心病猝死5例,非致死性心肌梗死16例,急诊再血管化4例,不稳定型心绞痛所致再住院27例。hs-CRP异常组较hs-CRP正常组冠状动脉事件发生率显著增高(RR1.90,95%CI1.09~3.32,P<0.05)。血脂异常组较正常组冠状动脉事件发生率亦显著增高(RR1.70,95%CI1.02~2.83,P<0.05)。血脂及hs-CRP均异常组较均正常组冠状动脉事件发生率显著升高(RR3.43,95%CI1.35~8.73,P<0.01)。结论:hs-CRP或(和)血脂水平异常的ACS患者6个月内冠状动脉事件发生率显著升高,并以二者均异常者为著,因此联合检测可能有助于识别冠心病二级预防中的高危个体。 相似文献