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Robbert-Jan Roozeman Lasse Murtomäki Kyösti Kontturi 《Journal of electroanalytical chemistry (Lausanne, Switzerland)》2005,575(1):9-17
A recently introduced setup to measure the dynamic interfacial tension of expanding drops was used to compare the adsorption behaviour of a series of lipids at the electrified water∣dichloroethane interface. Phospholipids with saturated carbon chains of different length (DMPC, DPPC, DSPC, DAPC, DBPC), an unsaturated phospholipid (DOPC) and an ethanolamine (DSPE) were compared. It was found that the adsorption decreases with increasing chain length. Also, the increase of the flow rate reduces the degree of adsorption effectively. On the timescale of the experiments, the DSPE, DAPC and DBPC adsorption showed no potential dependence, whereas the adsorption of DOPC was stronger than that of the saturated lipids. Adsorption was modelled using the Langmuir adsorption isotherm; the potential dependence of adsorption is discussed. 相似文献
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人体经络静电荷检测研究 总被引:3,自引:0,他引:3
在经络静电荷检测基础上,研制了高灵敏微型电荷传感器系统。在112名正常自愿者的十二经脉五腧合穴和啼阳明胃经静电荷检测,发现正常状态下经络呈负电性(-7.87×10^-3--.61×10^-13C),经脉失衡度〈9.06%;而在病理状态下(胃疾患)454例,经脉负电性小于正常人,失衡度在足三里穴达24.09%,稳定性差。又以胃电图离度(CV)分析,正常人空腹时,CV〈20-30%。胃疾患者CV〉30 相似文献
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Calis Sema Jeyanthi Ramasubbu Tsai Tsuimin Mehta Rahul C. DeLuca Patrick P. 《Pharmaceutical research》1995,12(7):1072-1076
Purpose. The interaction of salmon calcitonin (sCT) and poly (d,l-lactide-co-glycolide) was detected during preparation and evaluation of microspheres. The purpose of this study was to quantitate the extent and nature of the interaction.
Methods. Blank microspheres were prepared by an aqueous emulsification solvent extraction technique. Adsorption studies were carried out at six concentrations of sCT and three concentrations of microspheres. Adsorption isotherms were constructed using the Langmuir and Freundlich treatments.
Results. Adsorption at 1 mg/ml sCT concentration resulted in almost complete depletion of the peptide from the adsorption medium with the time to reach maximum adsorption decreasing with increasing microsphere concentration. At sCT concentrations below 100 µg/ml, a true equilibrium occurred in 1 hour or less while at higher concentrations (up to 350 µg/ml), a transient equilibrium was reached in 1 to 2 hours, followed by further adsorption of the peptide. The adsorption followed the Langmuir isotherm at concentrations below 200 µg/ml, indicating formation of a monolayer. Multilayer interaction, described by the Freundlich isotherm, occurred at higher concentrations and resulted in complete depletion of sCT from the adsorption medium. The affinity constant during monolayer formation was 0.09 and the plateau surface concentration was 5.1 µg/mg. The multilayer peptide-peptide adsorption showed a lower affinity (0.025) but higher capacity (24 µg/mg) than the monolayer peptide-polymer adsorption.
Conclusions. The results show that poly (d,l-lactide-co-glycolide) microspheres have a high adsorption capacity for sCT which must be considered in formulating a controlled delivery product of this peptide. 相似文献
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从制备方法着手综述了近年来聚苯胺/聚合物导电材料研制开发的最新成果,并简介了聚苯胺/聚合物导电材料的应用情况。 相似文献
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Dodd Steven W. Havel Henry A. Kovach Paul M. Lakshminarayan Chitra Redmon Martin P. Sargeant Charlene M. Sullivan Gary R. Beals John M. 《Pharmaceutical research》1995,12(1):60-68
Mixing pharmaceutical preparations of soluble neutral regular insulin solution (NRI) and neutral protamine Hagedorn (NPH) crystalline insulin suspension leads to a reduction in the measurable amount of soluble insulin in the formulation supernatant. However in spite of the loss in soluble insulin, the time-actions of these components have been shown, in clinical trials, to be unaffected. The interaction between these different physical forms of insulin has been studied using reversed-phase HPLC, isothermal titrating calorimetry, and Doppler electrophoretic light scattering analysis. Sorbent surface and solution perturbation studies revealed that the NRI adsorbs to the surface of the NPH crystal with an equilibrium constant ranging from 104 M–1 to 107 M–!, depending on the protamine concentration, pH, ionic strength, and temperature. This adsorption behavior suggests that the binding is mediated by electrostatic interactions arising between the positively-charged NPH crystal and the negatively-charged NRI hexamer. Doppler electrophoretic light scattering results, used to probe the pH-dependent surface charge of NPH and soluble insulin hexamer, support the conclusion that electrostatic interactions mediate the adsorption process. Adsorption studies under physiological conditions indicate that the elevated temperature and ionic strength, in a subcutaneous depot, are sufficient to lead to the dissociation of the NRI/NPH complex that exists in these NPH mixture formulations. 相似文献
8.
Surface-related triggering of the neutrophil respiratory burst. Characterization of the response induced by IgG adsorbed to hydrophilic and hydrophobic glass surfaces 总被引:1,自引:0,他引:1 下载免费PDF全文
L LIU H ELWING A KARLSSON G NIMERI C DAHLGREN 《Clinical and experimental immunology》1997,109(1):204-210
Hydrophilic and hydrophobic glass surfaces precoated with human albumin, fibrinogen, or IgG were investigated with respect to their ability to activate the neutrophil NADPH-oxidase. We found that IgG-coated surfaces induced a substantial and prolonged neutrophil production of reactive oxygen species (ROS). When a hydrophilic surface was used to support protein binding, a somewhat lower neutrophil response (around 35%) was obtained, compared with the response induced by IgG on a hydrophobic surface. The production of ROS was completely eliminated when cytochalasin B was added to the measuring system, suggesting the involvement of the cell cytoskeleton in the activation process. The relation between the intra- and extracellular generation of ROS was further assessed, and we found that most of the ROS produced were released from the cells, in agreement with a model in which the activating surfaces induce a ‘frustrated’ phagocytic response. Serum totally inhibited ‘frustrated’ phagocytosis provided that the IgG molecules were sticking to a hydrophilic surface. 相似文献
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Zhanjun Zhang Jing Huang Xizum Wu Wenzhi Zhang Songying Chen 《Journal of electroanalytical chemistry (Lausanne, Switzerland)》1998,444(2):169-172
The electrochemical reduction of adrenochrome on a glassy carbon electrode was investigated by ac impedance in a constant ionic strength McIlvaine buffer solution. When the frequency was lower than 60 kHz, —Z″ against Z′ showed straight lines whose inclined angles with the real axis were within the range 71–76° in the diagrams of the complex impedance plane obtained at different do potentials. In the corresponding admittance diagrams, distorted semicircles appeared. The electrode reaction involves adsorption of electroactive species and the effect of concentration polarization cannot be neglected. Based upon the admittance model of the equivalent circuit, the optimal estimated values of some model parameters were obtained by using a Gauss-Newton algorithm and non-linear curve fitting. The characteristics of the electrode process are also discussed. 相似文献
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