叶下珠复方治疗慢性乙型肝炎54例临床观察

应用叶下珠复方治疗慢性乙型病毒性肝炎５４例，与灭澳灵１０例作对照。结果显示叶下珠复方组治疗３个月（其中１０例治疗６个月）．临床症状体征显著改善，肝功能复常或明显改善者达８２．６％；ｅ抗原及ＨＢＶ—ＤＮＡ转阴率分别为４６．２％（２４／５２）和４６．３％（１９／４１）；疗效优于灭澳灵组（上述３项指标分别为２０％，１０％，１００％；Ｐ＜０．０１和Ｐ＜０．０５）。叶下珠复方组治疗３个月后ｅ抗原及ＤＮＡ未阴转中１０例延长疗程至６个月，结果ｅ抗原及ＨＢＶ—ＤＮＡ转阴分别为６例；ｅ抗原阴转患者中大部分伴随ＨＢＶ—ＤＮＡ阴转（８５．７％）及肝功能复常或改善（１００％）。ｅ抗原及ＮＢＶ—ＤＮＡ的阴转率似在成年患者及病程短者较高；而与中医证型似无明显关系。研究表明叶下珠复方治疗慢性乙型病毒性肝炎具有一定的疗效，延长疗程似可提高疗效，尚需进一步研究。     

巴金森氏病智力损害与年龄、病程、运动障碍的关系

巴金森氏病病人的年龄、起病年龄、病程、运动障碍的严重程度与其智力损害的关系至今仍存有争议。为此用定量的临床评定方法、标准化的韦氏成人智力测验对31名连续收选的特发性巴金森氏病进行了研究。结果发现缓动的严重程度与智力损害明显相关,而震颤严重程度与智力损害呈反相关。病人的年龄、起病年龄、病程及强直的程度与智力损害无明显相关。这些结果提示引起缓动的皮层下脑损害同样可能引起智力损害,而有明显震颤的巴金森氏病可能属于该病的早期或属于智力损害较轻的一种巴金森氏病亚型。     

Novel porous aortic elastin and collagen scaffolds for tissue engineering

Decellularized vascular matrices are used as scaffolds in cardiovascular tissue engineering because they retain their natural biological composition and three-dimensional (3-D) architecture suitable for cell adhesion and proliferation. However, cell infiltration and subsequent repopulation of these scaffolds was shown to be unsatisfactory due to their dense collagen and elastic fiber networks. In an attempt to create more porous structures for cell repopulation, we selectively removed matrix components from decellularized porcine aorta to obtain two types of scaffolds, namely elastin and collagen scaffolds. Histology and scanning electron microscopy examination of the two scaffolds revealed a well-oriented porous decellularized structure that maintained natural architecture of the aorta. Quantitative DNA analysis confirmed that both scaffolds were completely decellularized. Stress-strain analysis demonstrated adequate mechanical properties for both elastin and collagen scaffolds. In vitro enzyme digestion of the scaffolds suggested that they were highly biodegradable. Furthermore, the biodegradability of collagen scaffolds could be controlled by crosslinking with carbodiimides. Cell culture studies showed that fibroblasts adhered to and proliferated on the scaffold surfaces with excellent cell viability. Fibroblasts infiltrated about 120 microm into elastin scaffolds and about 40 microm into collagen scaffolds after 4 weeks of rotary cell culture. These results indicated that our novel aortic elastin and collagen matrices have the potential to serve as scaffolds for cardiovascular tissue engineering.     

针刺、自主牵引、药物外敷治疗腰椎间盘突出症疗效观察

目的 :观察针刺、自主牵引、药物外敷治疗腰椎间盘突出症 (简称治疗组 )的疗效。方法 :随机分为针刺、自主牵引、药物外敷组和单纯针刺组进行对比观察。结果 :治疗组总有效率 94 .1% ,明显高于对照组 75 .8% ,经统计学处理 ,P <0 .0 5 ,具有显著性差异。结论 :治疗组疗效优于对照组     

诱导型一氧化氮合酶和内皮型一氧化氮合酶在胃癌中的表达关系及临床意义

目的检测诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)在小鼠正常胃组织及原位移植人胃癌组织SGC-7901中的表达情况,探讨严重联合免疫缺陷(SCID)小鼠胃癌不同阶段时iNOS和eNOS含量变化的相关性研究及临床意义。方法采用苏木精-伊红染色法进行检测。结果可见eNOS和iNOS在10只正常胃组织的相关性比较弱,30只胃癌组织中,25只iNOS表达较正常胃组织明显升高差异有统计学意义(83%和38%,P<0.05),iNOS与胃癌的发生发展有关系。25只胃癌组织中有17只eNOS表达略高于正常胃癌组织(57%和20%,P<0.05)。iNOS与eNOS在胃癌组织中的表达有相关性(P<0.01)。结论eNOS和iNOS之间可能存在相互作用共同参与了胃癌的发展转移及浸润。     

GLP-1及其类似物对减缓骨质疏松作用的研究进展

骨质疏松是糖尿病的并发症之一,故降低血糖的同时也不能忽视对骨质疏松的防治。近年来的一些研究发现,胰高血糖素样肽-1（glucagon like peptide 1,GLP-1）不仅在控制血糖方面发挥重要作用,而且在减缓糖尿病合并骨质疏松方面也有疗效。GLP-1及其类似物可改善机体高血糖状态,抑制骨质吸收;并通过破骨细胞核因子κ B受体活化因子配体（receptor activator for nuclear factor-κ B ligand,RANKL）通路,阻止破骨细胞的成熟,减缓破骨作用;还可调节成骨细胞Wnt通路,影响Wnt／β-catenin比例,促进成骨细胞的分化与成熟,有利于骨质沉积;在间充质干细胞（bone mesenchymal stem cells,BMSC）的分化亦起到调节作用,促使其向成骨细胞分化,减少向脂肪细胞分化;GLP-1及其类似物可以减少晚期糖基化终末产物（advanced glycationend products,AGEs）在骨胶原中沉积,避免破骨细胞的过度增值与分化,此外GLP-1及其类似物还通过其他途径保护骨代谢,减缓骨质疏松的进展。     

Long-range protein electron transfer observed at the single-molecule level: In situ mapping of redox-gated tunneling resonance

A biomimetic long-range electron transfer (ET) system consisting of the blue copper protein azurin, a tunneling barrier bridge, and a gold single-crystal electrode was designed on the basis of molecular wiring self-assembly principles. This system is sufficiently stable and sensitive in a quasi-biological environment, suitable for detailed observations of long-range protein interfacial ET at the nanoscale and single-molecule levels. Because azurin is located at clearly identifiable fixed sites in well controlled orientation, the ET configuration parallels biological ET. The ET is nonadiabatic, and the rate constants display tunneling features with distance-decay factors of 0.83 and 0.91 A(-1) in H(2)O and D(2)O, respectively. Redox-gated tunneling resonance is observed in situ at the single-molecule level by using electrochemical scanning tunneling microscopy, exhibiting an asymmetric dependence on the redox potential. Maximum resonance appears around the equilibrium redox potential of azurin with an on/off current ratio of approximately 9. Simulation analyses, based on a two-step interfacial ET model for the scanning tunneling microscopy redox process, were performed and provide quantitative information for rational understanding of the ET mechanism.     

骨巨细胞瘤的综合分期法的临床价值

目的评价骨巨细胞瘤的临床、Ｘ线、病理学表现及三者结合综合分期的意义。方法收集经手术病理证实的骨巨细胞瘤７８例,以其临床、Ｘ线、病理特征为依据,进行临床分症,Ｘ线分型、病理分级三结合回顾性观察分析,将骨巨细胞瘤综合分为三期。结果三项均符合者６４例,占８２．０％。对Ⅰ、部分Ⅱ期的治疗选择瘤体刮除烧灼加植骨,部分Ⅱ期采用瘤体整块切除加植骨治疗共５６例,随访５年４例复发。对Ⅲ期患者采取截肢或瘤体整块切除加骨水泥充填,５年生存率５０．０％（６／６）。结论骨巨细胞瘤的三结合综合分期法对选择适当的治疗方案有重要的临床指导意义。     

手术助产分娩与儿童智能发育关系的配对双盲研究

对剖宫产、胎头吸引、臀位牵引等手术助产分娩的3～7岁儿童采用配对双盲法探讨其对儿童智能发育的影响.结果表明:胎头吸引术对儿童的记忆能力和思维能力有显著影响,而剖宫产术和臀位牵引术对儿童的智能发育无明显影响.     

肝纤维化中医药治疗临床探讨

从肝纤维化这一概念入手,参考中医传统典籍中胁痛、积聚、鼓胀、肝着、肝积等病证特征,认为临床以痰瘀阻络为主要病理表现,但临床治疗不能一味攻痰逐瘀,应注重攻补兼施,同时兼顾兼证,辨证论治.