HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells

Objective Lung cancer cells associated with radioresistance are likely to give rise to local recurrence and distant metastatic relapse,but little is known about its underlying mechanisms.In the present paper,the effects of the HPV16 E6 and HPV16 E7 oncoprotein on the radiosensitivity of lung cancer cell lines were investigated.Methods The HPV16 E6 or HPV16 E7 oncoprotein was expressed by a transient transfection with pcDNA3-HPV16 E6 or pcDNA3-HPV16 E7 expression vector.Human lung cancer H2179 cells and mouse lung cancer Lewis cells were exposed to a γ-ray radiation source,cellular survival was evaluated by using a colony formation assay.The expression of HPV16 oncoproteins E6/E7,extracellular signal-regulated kinases 1/2(ERK1/2) and AKT signaling was determined by Western blot assay.VEGF secretion was determined by ELISA.Results Both HPV16 oncoproteins E6 and E7 significantly decreased radiosensitivity of H2179 cells,associated with a promotion of the ERK1/2 and AKT phosphorylation.A decrease of reactive oxygen species(ROS) and an increase of VEGF levels were observed in the cells expressing the HPV16 oncoproteins E6 and E7.Furthermore,a similar reduction of radiosensitivity mediated by the HPV16 oncoproteins E6 and E7 was also observed in a mouse lung cancer Lewis cells.Conclusion The findings indicate that the HPV16 oncoproteins E6 and E7 negatively affects susceptibility of lung cancer cells to radiotherapy via regulation of the ERK1/2 and Akt signaling pathway and VEGF expression.     

基层医院放射科CR系统QA、QC浅谈

CR(computed radiography,计算机X线成像)系统的广泛应用,明显提高了放射科X线图像质量,减少了胶片浪费,提高了经济效益和工作效率.     

Long Non-coding RNAs in Response to Genotoxic Stress

Long non-coding RNAs(lncRNAs) are increasingly involved in diverse biological processes.Upon DNA damage,the DNA damage response(DDR) elicits a complex signaling cascade,which includes the induction of lncRNAs.LncRNA-mediated DDR is involved in non-canonical and canonical manners.DNA-damage induced lncRNAs contribute to the regulation of cell cycle,apoptosis,and DNA repair,thereby playing a key role in maintaining genome stability.This review summarizes the emerging role of lncRNAs in DNA damage and repair.     

骨肉瘤侵犯骨骺及关节的低场MR表现

目的 探讨骨肉瘤对骨骺和/或关节侵犯的低场MRI表现.方法 复习12例骨肉瘤的MR影像学特点,并与X线平片进行对比分析.结果 12例中,7例骨骺未闭合的患者中有5例越过骺板侵及骨骺,5例骨骺闭合的患者中有4例侵及骨性关节面.MRI显示其病变范围均明显大于X线片.结论 MRI是判断骨肉瘤患者骨骺和/或关节受侵情况的可靠方法,是术前不可缺少的补充检查.     

红光照射对人体抗疲劳能力的影响

目的 采用红光照射后,检测心肌酶谱、凝血系列和血常规相关分子的含量,研究红光照射对人体抗疲劳能力和耐力的影响.方法 将200名实验对象分为实验组和对照组,每组100例.实验组受试者在力竭运动后接受红光照射,每次照射30min,剂量为30J/cm2,每天1次,持续2周;对照组受试者在相同条件下进行假照射.第3、7、14天后分别测定实验组和对照组受试对象血内心肌酶谱AST、ADH、CK、CK-MB、HBDH,血常规WBC、HB、RBC、PLT和凝血系列PT、APTT、FIB、TT及血钙等各项指标的含量.结果 与对照组相比,经红光照射后实验组血常规中的HB、RBC和Ca2 含量明显增高,其他指标未见明显改变.结论 红光照射可明显提高人体的耐力和抗疲劳能力.     

莱姆病的防治

莱姆病在林区较多见,深入了解并积极预防是基层,特别是边远、林区部队的重要任务.莱姆病为蜱传伯氏疏螺旋体所致之自然疫源性疾病,潜伏期3～32天,典型表现为叮咬部位出现的慢性游走性红斑,同时可伴有低热、头痛及全身乏力等一些感冒样症状,后期可累及心脏、关节、皮肤和神经系统等.通常特异性IgM抗体多在游走红斑发生后2～4周出现,6～8周达高峰,4～6个月降至正常水平,特异性IgG抗体多于病后6～8周开始升高,4～6个月达高峰,可持续数年以上.     

经鼻、食管引流及覆膜支架植入术治疗食管-胸腔瘘

目的 探索经鼻、食管引流及覆膜支架植入术治疗食管-胸腔瘘的可行性及临床价值.方法 7例食管胸腔瘘患者采用5 F猪尾巴多侧孔导管经鼻、食管行胸腔引流,并利用食管覆膜内支架封堵食管瘘口.术后经引流导管定时冲洗脓腔,复查脓腔造影.结果 患者一次性植入引流导管及堵瘘支架均获得成功;引流持续12～22 d,平均15 d;拔管前引流管造影提示脓腔明显变小或闭塞、液气胸得到明显控制,拔管后食管造影示瘘口封堵满意、支架完全复张,未见明显移位、狭窄等征象.结论 经鼻、食管引流及覆膜支架植入术治疗食管胸腔瘘,技术上可行,操作简易、安全,临床疗效肯定.     

系统生物技术——组学时代的代谢工程

高通量实验技术的广泛应用产生了海量数据,利用生物信息学工具整合这些数据,加深了人们对细菌生理活动规律的认识,系统生物学应运而生.系统生物学的出现使代谢工程从局部通路水平上升到整体水平,代谢工程也因此进入了新的发展阶段--系统生物技术时代.系统生物技术通过整合各个层次组学数据,建立数学模型,或通过比较不同菌株或同一菌株在不同条件下基因组、转录组、蛋白组或代谢组的差异以阐明生命活动规律,在此基础上,对影响表型的靶基因进行改造,得到符合预期的表型.随着新的高通量实验技术的不断运用以及生物信息学的发展,系统生物技术必将取得更大进步.     

《常见危重综合征救治》

本书是一本全面介绍ICU常见危重病综合征的专业书籍,邀请多年在第一线工作的专家、教授,依据他们各自的临床实践经验和体会,结合国内外相关的医学新进展而撰写。依据系统分10章。每个综合征自成一节,内容包括概述、机制、病理解剖、临床表现、诊断、鉴别     

"不怕死"与"很健康"

一次文友笔会时,一位年轻的诗人慷慨激昂地说:国人缺乏一种视死如归的气概,如果人人都不畏惧死亡,那我们还有什么宏愿不能成真?那你做得怎么样呢?有人半开玩笑地问。可以说,我是不怕死的。如果我是一个军人,战争时我一定会赴汤蹈火,冲     

Optimization of the system of medical service of students of Cadet Schools of the Ministry of Defense of the Russian Federation

One of the factors of the successful military career guidance Cadet schools students is preserving and promoting their health. Medical support of children and adolescents aged 10-17 years should include the full range of medical and preventive measures defined for this group. The state of providing outpatient care for pupils at the Cadet School in St. Petersburg was studied. These results show that full medical care in accordance with the standards can be based only on children's health clinics. It is important that the organization of medical support pupils cadet schools should be cooperate with civilian health care.     

带状疱疹83例误诊原因分析

带状疱疹是由水痘—带状疱疾病毒引起的皮肤科常见疾病。其主要的病理损害,一是受累神经的严重炎症性浸润,继而导致受侵犯神经节内神经细胞变性、坏死;二是皮肤的水泡。迅速抑制神经节和相应的感觉神经纤维的充血、水肿和坏死,防止粘连形成,达到迅速镇痛、改善皮损,缩短病程及防止后遗症的发生是治疗的关键。因而,尽早明确诊断,     

Estimation of accumulated dose of radiation by method of ESR-spectrometry of dental enamel of mammals

ESR-spectrometry was used to investigate radiation-induced paramagnetic centers in enamel of mammals: carnivores (polar bear and fox), ungulates (reindeer, European bison, moose), and man. Values at half the microwave power saturation of the radiation signal, P_1/2, evaluated at room temperature, was found to range from 16 to 26 mW for animals and man. A new approach to discrimination of the radiation induced signal from the total ESR spectrum of reindeer enamel is proposed. ‘Dose-response’ dependencies of enamel of different species mammals were measured within the dose range from 0.48 up to 10.08 Gy. Estimations of ‘radiosensitivity’ enamel of carnivores and ungulates showed good agreement with radiosensitivity enamel of man by ESR method.     

中草药治疗奶牛胎衣不下的效果体会

胎衣不下指奶牛在产后胎衣不能在正常时间内顺利排下,本文阐述了造成病因的病理,以及提出了中草药治疗此病的方法。     

耳廓软骨开窗法治疗耳廓假性囊肿25例体会

目的介绍耳廓软骨开窗法治疗耳廓假性囊肿的步骤、优点和操作体会。方法手术在局麻下进行,切口均选在耳廓前面,沿与耳轮大致平行方向作一贯穿囊肿之切口,将皮肤与囊肿表层软骨分离,切开囊肿表层软骨,吸净囊液,再以眼科剪将表层囊壁软骨大部分切除,以碘伏棉签轻拭囊腔,酒精棉签脱碘,缝合皮肤切口,适度加压包扎。术后换药1～2次,6～8d拆线。结果25例患者切口均Ⅰ期愈合,无感染或其它并发症。随访6～36个月,均无复发,耳廓无增厚硬化或其他畸形,外观效果满意。结论耳廓软骨开窗法治疗耳廓假性囊肿是一种疗效肯定的方法。     

从髌股关节的生物力学评价髌骨骨折的治疗

从髌股关节的生物力学分析176例髌骨骨折。髌骨骨折后疗效的好坏与髌股关节的生物力学特点及髌骨功能恢复的程度密切相关。治疗髌骨骨折的原则应是保留髌骨，并充分恢复其解剖关系。