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Lu Lu Qinghui Meng Ming Cui Xiaofei Chu Shuyi Zhao Huiwen Xiao Jiali Dong 《国际放射医学核医学杂志》2016,40(2):85-90
Objective Lung cancer cells associated with radioresistance are likely to give rise to local recurrence and distant metastatic relapse,but little is known about its underlying mechanisms.In the present paper,the effects of the HPV16 E6 and HPV16 E7 oncoprotein on the radiosensitivity of lung cancer cell lines were investigated.Methods The HPV16 E6 or HPV16 E7 oncoprotein was expressed by a transient transfection with pcDNA3-HPV16 E6 or pcDNA3-HPV16 E7 expression vector.Human lung cancer H2179 cells and mouse lung cancer Lewis cells were exposed to a γ-ray radiation source,cellular survival was evaluated by using a colony formation assay.The expression of HPV16 oncoproteins E6/E7,extracellular signal-regulated kinases 1/2(ERK1/2) and AKT signaling was determined by Western blot assay.VEGF secretion was determined by ELISA.Results Both HPV16 oncoproteins E6 and E7 significantly decreased radiosensitivity of H2179 cells,associated with a promotion of the ERK1/2 and AKT phosphorylation.A decrease of reactive oxygen species(ROS) and an increase of VEGF levels were observed in the cells expressing the HPV16 oncoproteins E6 and E7.Furthermore,a similar reduction of radiosensitivity mediated by the HPV16 oncoproteins E6 and E7 was also observed in a mouse lung cancer Lewis cells.Conclusion The findings indicate that the HPV16 oncoproteins E6 and E7 negatively affects susceptibility of lung cancer cells to radiotherapy via regulation of the ERK1/2 and Akt signaling pathway and VEGF expression. 相似文献
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Biomarkers,such as chromosome aberration and micronuclei assays,prove to be reliable for facilitating clinical diagnosis in radiation accidents.In a radiation accident in India,chromosomal aberration,γ-H2AX,as well as other blood markers,were detected in accidentally exposed victims.This multi-parametric approach aided in confirming that individuals had been exposed by ionizing radiation.However,doses were impossible to estimate because of a 30-day delay in accident awareness.Exposure dose for victims was estimated using a dose-response curve previously established.Dose estimation,blood cell depletion kinetics,and no appearance of prodromal symptoms suggested that doses of exposure were low.Hematologic investigation,sampling time,and chromosome aberration scoring were all proposed according to data from the victims exposed to 60Co.Finally,knowledge regarding chromosome aberration analysis and the importance of international co-operation and assistance should be shared from this accident. 相似文献
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目的 观察补充维生素B2对急性低氧暴露小鼠外周血代谢组的影响.方法 采用随机数字表法将35只雄性昆明小鼠随机分为:正常对照组,低氧对照组,2倍、4倍及8倍维生素B2补充组,每组7只.以相应饲料(维生素B2含量分别为6 mg/kg、12 mg/kg、24 mg/kg和48 mg/kg)喂养2周后,除正常对照组外,其它各组均在模拟6000 m高度停留8 h,采集血浆,以核磁共振的方法分析其代谢组变化.结果 急性低氧暴露后,各组动物血浆代谢组在得分图中呈聚类型分布,且有先分离后同归的代谢模式变化轨迹,显示出补充不同剂量维生素B2后小鼠血浆代谢组逐渐恢复的趋势.代谢模式产生差别的原因是脂类、乳酸、丙氨酸、N-乙酰糖蛋白、谷氨酸、胆碱、牛磺酸、糖、肉碱、甘氨酸和肌酐等物质的水平发生了变化,表明相关的代谢途径发生了变化.结论 补充维生素B2使急性低氧暴露机体碳水化合物、脂肪、蛋白质代谢发生改变,而维生素B2可以改善碳水化合物代谢,并可能通过肉碱间接调节了脂肪代谢;同时还发现一些氨基酸代谢发生显著变化.
Abstract:
Objective To observe the effects of vitamin B2 supplementation on plasma metabonome of the mice which exposed to acute hypoxia. Methods Thirty-five male Kunming mice were randomly and averagely divided into 5 groups, among which the control and hypoxia control groups were fed the fodder containing 6, 12, 24, 48 mg/kg vitamin B2 respectively. All groups were exposed to simulated hypoxia environment (equivalent to 6000 meters above sea level) for 8 hours except control group. Nuclear magnetic resonance spectrometer was used to test the change of metabonome from collected plasma. Results After acute hypoxia exposure, the metabonome pattern in all groups showed clustering distribution in scores plot and the metabonome changes were along the trail from segregation to regression. These indicated a gradual recover tendency on plasma metabonome when different doses of vitamin B2 supplemented. The changes of lipids, lactic acid,alanine, N-acetyl-glycoprotein (NAC), glutamate, choline, taurine, glucose, carnitine, glycine and creatinine were among the factors contributing significantly to the difference of metabonome pattern and indicated the changes of related metabolic pathway. Conclusions The metabonomes of carbohydrate, lipid and protein of acute hypoxia mice are improved by vitamin B2 supplementation.Vitamin B2 is helpful to improve carbohydrate metabolism and indirectly accommodate lipid metabolism through carnitine. Vitamin B2 supplementation also results in the significant change of amino acid metabonome. 相似文献
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目的 初步探索皮下应用Gd-DTPA-白蛋白的阳性磁共振淋巴造影检测隐匿转移性淋巴结的价值.方法 建立腘窝隐匿转移性淋巴结新西兰兔模型,分析隐匿转移性淋巴结在平扫及皮下注射0.10 mmol/肢Gd-DTPA-白蛋白后24 h时的MRI特征,并与病理检查对照.结果 12只兔的腘窝隐匿转移性淋巴结模型成功建立,其淋巴结大小为(6.7±0.2)mm.平扫时隐匿转移性腘窝淋巴结表现为T1WI上等信号,T2WI上高信号,与正常淋巴结相似.Gd-DTPA-白蛋白增强24 h,正常淋巴结呈明显、均匀的强化,而隐匿转移性淋巴结呈不均匀强化,强化形式有环状(4枚淋巴结)、云絮状(3枚)和不规则偏心"充盈缺损"(3枚),另有2枚淋巴结不强化.在脂肪抑制T1WI像上淋巴管呈明亮高信号,转移性淋巴结的引流淋巴管明显迂曲扩张.结论 皮下应用Gd-DTPA-白蛋白的阳性MR淋巴造影可清晰显示淋巴结和淋巴管,可用于检测隐匿转移性淋巴结. 相似文献
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1病历简介
患者,男,43岁。因间断性排尿困难半月余,伴尿频、尿急、尿痛入院。患者于12年前因骨盆骨折合并尿道断裂在我院行尿道修补术,术后排尿功能良好。术后第3年出现排尿困难,经2次尿道扩张术后,排尿正常。查体:体温37.5℃,心、肺、腹无异常。外科情况:双脊肋角不饱满,双肾区轻微扣击痛,沿输尿管走行无压痛及扣击痛,下腹部正中可见8.0cm长的纵向愈合伤口。 相似文献
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One of the factors of the successful military career guidance Cadet schools students is preserving and promoting their health. Medical support of children and adolescents aged 10-17 years should include the full range of medical and preventive measures defined for this group. The state of providing outpatient care for pupils at the Cadet School in St. Petersburg was studied. These results show that full medical care in accordance with the standards can be based only on children's health clinics. It is important that the organization of medical support pupils cadet schools should be cooperate with civilian health care. 相似文献
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V. A. Serezhenkov I. A. Moroz G. A. Klevezal A. F. Vanin 《Applied radiation and isotopes》1996,47(11-12)
ESR-spectrometry was used to investigate radiation-induced paramagnetic centers in enamel of mammals: carnivores (polar bear and fox), ungulates (reindeer, European bison, moose), and man. Values at half the microwave power saturation of the radiation signal, P1/2, evaluated at room temperature, was found to range from 16 to 26 mW for animals and man. A new approach to discrimination of the radiation induced signal from the total ESR spectrum of reindeer enamel is proposed. ‘Dose-response’ dependencies of enamel of different species mammals were measured within the dose range from 0.48 up to 10.08 Gy. Estimations of ‘radiosensitivity’ enamel of carnivores and ungulates showed good agreement with radiosensitivity enamel of man by ESR method. 相似文献
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海马头部浅沟消失对海马硬化诊断价值的探讨 总被引:3,自引:0,他引:3
目的 探讨海马头部浅沟消失对海马硬化的诊断价值。方法 对 18例经组织学检查证实的海马硬化患者的MRI检查资料和 18例年龄相匹配的对照组进行回顾性分析 ,观察海马头部浅沟的显示情况、海马头部大小和信号改变。结果 18例海马硬化患者中 ,16例硬化侧海马头部浅沟消失 ,1例硬化侧海马头部浅沟明显变浅 ,几乎消失 ,1例硬化侧海马头部浅沟存在。硬化侧海马头部均有萎缩 ,并在T2 WI和液体衰减恢复 (FLAIR)成像呈高信号。海马头部浅沟消失对海马硬化诊断的敏感性为 88.9% ,特异性为 10 0 %。结论 海马头部浅沟消失是诊断海马硬化的一个可靠征象 ,结合患侧海马有萎缩性改变和T2 加权成像信号增高 ,可肯定诊断海马硬化。 相似文献
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目的观察、探讨湿润烧伤膏(MEBO)治疗婴儿湿疹的临床疗效。方法将84例婴儿湿疹患儿随机分为治疗组(44例)和对照组(40例),治疗组外涂湿润烧伤膏治疗,对照组外涂湿疹膏治疗,连续治疗1周后,观察两组疗效。结果治疗组有效率为97.73%,对照组有效率为82.50%;两组疗效经秩和检验,差异具有统计学意义(P0.05);随访1个月,治疗组未见复发,对照组有2例患儿复发。结论湿润烧伤膏治疗婴儿湿疹疗效好,无毒副作用,安全可靠。 相似文献