HPLC法测定桃仁膝康丸中芍药苷的含量

目的建立桃仁膝康丸中芍药苷的含量测定方法。方法采用HPLC法测定芍药苷的含量。色谱柱Agilent C18柱(4.6mm×250mm,5μm),流动相:乙腈-水(13∶87),流速1.0 mL/min,柱温30℃,检测波长230nm。结果芍药苷进样量在0.6128～3.0640μg范围内呈良好的线性关系(r=0.9999),平均加样回收率为99.28%,RSD=1.59%。结论本法操作简便,重现性好,可用于桃仁膝康丸的质量控制。     

复肾康丸的薄层色谱鉴别

目的建立复肾康丸的质量控制方法。方法采用薄层色谱法对制剂中的黄芪、丹参、川芎进行定性鉴别。结果薄层色谱法可鉴别出黄芪、丹参、川芎。结论薄层色谱法专属性强，简便可行，可用于复肾康丸的质量控制。     

桃红通瘀丸的质量控制标准研究

目的 采用薄层鉴别法和高效液相色谱法建立桃红通瘀丸质控方法用于产品质量控制.方法 采用薄层色谱法定性鉴别桃红通瘀丸中的川芎、北柴胡、醋延胡索,采用高效液相色谱法测定桃红通瘀丸桃仁中苦杏仁苷的含量.结果 采用薄层色谱法确定了川芎、北柴胡、醋延胡索定性鉴别方法;采用高效液相色谱法建立了产品含量测定方法,苦杏仁苷在15.83...     

肾康丸的鉴别

目的建立肾康丸中黄芪、山楂、金樱子的鉴别方法。方法采用TLC法,在硅胶G薄层板上。以氯仿-甲醇-水(13：7：2)为开展开剂鉴别了黄芪;以正己烷．氯仿-乙酸乙酯(20：5：8)为展开剂鉴别了山楂;以甲苯．乙酸乙酯．甲酸(5：4：1)为展开剂鉴别了金樱子。结果TLC色谱可用于肾康丸中黄芪、山楂、金樱子的鉴别。结论所建立的方法专一、简便、可靠,可用于肾康丸的质量控制。     

康肾丸质量控制的研究

目的：建立康肾丸的质量控制方法。方法：采用薄层色谱法（TLC）对方中主要药材黄芪、虫草菌丝粉、防风分别进行定性鉴别；采用高效液相色谱法（HPLC）测定黄芪甲苷的含量。结果：定性鉴别方法重现性好，专属性强；含量测定的加样回收率为99．39％，RSD为0．59％。结论：本方法简单、易行、精密度高、重复性好，能够有效地控制康肾丸的质量。     

麒麟丸的薄层色谱鉴别

目的：研究建立麒麟丸质量控制方法。方法：采用薄层色谱法对麒麟丸中的主药淫羊藿、白芍进行鉴别。结果：薄层色谱具有鉴别特征斑点,易于识别,重现性好。结论：本实验制定薄层色谱方法可应用于麒麟丸的质量控制。     

薄层色谱法控制白驳丸质量

目的建立黄芪、当归在白驳丸中的薄层鉴别方法.方法采用薄层色谱法对复方制剂白驳丸中的黄芪、当归进行鉴别.结果白驳丸中的黄芪、当归在薄层色谱中斑点清晰,易于识别.结论薄层色谱法控制白驳丸质量准确可靠.     

太极升降丸的薄层色谱鉴别

目的制订太极升降丸的薄层色谱鉴别方法,以控制其质量。方法采用薄层色谱法对方中大黄、冰片和姜黄进行定性鉴别。结果在薄层色谱中可分别检出大黄、冰片和姜黄的特征斑点。结论所建立的方法专属性强,可用于太极升降丸的质量控制。     

蜂房丸的薄层色谱鉴别

目的探讨蜂房丸的定性鉴别方法。方法采用薄层色谱法对蜂房丸中主要药材进行鉴别。结果蜂房丸中的青皮、柴胡、甘草、苦甘草等4味药材可采用薄层色谱法进行特异性鉴别。结论所建立的薄层色谱法可有效地控制蜂房丸的质量。     

康瘫丸的质量标准

目的：建立康瘫丸的质量标准。方法：用薄层色谱法对康瘫丸中的黄芪、赤芍、羌活、当归进行定性鉴别;HPLC法测定制剂中芍药苷含量;检测波长230nm,流速1．0ml·min^-1。结果：薄层色谱斑点清晰,芍药苷在21．6～216．0μg·ml^-1浓度范围内线性关系良好（r=0．9999）,平均回收率为99．5％,RSD为1．15％（n=6）。结论：本方法简便、准确,可控制该制剂的质量。     

茜草炭的质量标准提升研究

目的在现行中国药典中茜草炭标准的基础上增加薄层鉴别和含量测定。方法该研究于 2022年 6—8月以 10批茜草和相应的茜草炭为研究对象,鉴别研究采用薄层色谱法,含量测定采用液相色谱法。结果茜草炭薄层色谱中,在与异茜草素对照品色谱相应的位置上,显相同颜色的荧光斑点,可明显区分茜草和茜草炭;含量测定以异茜草素为指标,在 8.2~820.0 mg/L范围内与峰面积呈线性关系( r=0.999 9),精密度、稳定性和重复性良好( RSD分别为 0.6%、1.8%和 2.4%),平均加样回收率 99.1%。结论建立的薄层鉴别和含量测定方法简便可行,可为茜草炭的生产及质量控制提供依据。     

舒肝健胃丸薄层色谱鉴别方法研究

目的:建立舒肝健胃丸的延胡索薄层色谱(TLC)鉴别方法。方法:对延胡索进行薄层色谱鉴别。结果:薄层色谱斑点清晰,阴性对照无干扰。结论:所用方法简便,重现性好,可用于舒肝健胃丸的质量控制。     

巴戟甲素亲水凝胶骨架肠溶缓释片的质量研究

目的:建立巴戟甲素亲水凝胶骨架肠溶缓释片质量控制方法。方法:按中国药典附录ⅠA片剂项下要求依次检查片重,采用转篮法检查制剂的释放度;采用薄层色谱法鉴别制剂中巴戟甲素,采用高效液相-蒸发光色谱法(HPLC-ELSD)测定巴戟甲素含量。结果:制剂片重和释放度均符合制剂通则标准;制剂巴戟甲素薄层行为良好,且阴性对照无干扰;测得5批制剂中巴戟甲素含量在38.33～40.89 m.g片-1之间,为标示量的93.0%～107.0%。结论:制剂指标检查符合制剂通则标准;所建立的薄层色谱法可准确鉴别制剂中的巴戟甲素;制剂中巴戟甲素含量符合规格要求,研制的骨架肠溶包衣缓释片质量可控、稳定。本方法准确、可靠、重复性好,可用于巴戟甲素亲水凝胶骨架肠溶缓释片的质量控制。     

The choice of paper and thin-layer chromatographic systems for the analysis of basic drugs

Thirty-seven paper and thin-layer chromatographic systems in general use for the analysis of basic drugs have been examined. Their discriminating powers were measured, both individually and when used in combination. The better systems were found to be thin-layer systems of silica gel sprayed with 0·1n NaOH, dried and run using one of the following solvents: (1) chloroform-methanol (90:10), (2) cyclohexane-toluene-diethylamine (75:15:10) and (3) acetone. A thin-layer cellulose system using n-butanol-water-citric acid (87:13: 0–48) was suitable if speed was not a requirement and a reversed phase paper system run with an aqueous buffer solution (pH 4·58) at 95? was the fastest system examined. Any of these five systems could be used in combination since their correlation coefficients were never higher than 0·61.     

Development and evaluation of the tablets coated with the novel formulation termed thin-layer sugarless coated tablets

The purpose of this study was to develop and evaluate the thin-layer sugarless coated tablets containing Vitamin C, Vitamin E, Vitamin B2, calcium pantothenate, and L-cysteine. As a result of the formulation study, three coating layers, 2% under coating (UC), 38% build-up coating (BC), and 5% syrup coating (SC) were necessary for sufficient impact toughness, elegant appearance, and improvement of appearance stability after storage at 25 degrees C/75% RH for 6 months under open conditions. We demonstrated that the thin-layer sugarless coated tablets are superior to the sugar-coated tablets in terms of small tablet size and stability of calcium pantothenate. It was due to the coating method, the continuous spray mist method, which can minimize the thicknesses of coating layers and the moisture content in the tablets. We also demonstrated that the thin-layer sugarless coated tablets are superior to the film-coated tablets in terms of masking ability of the unpleasant odor and the appearance, stability of the appearance, and low hygroscopicity. It was due to the dense, opaque, and stable coating layers mainly consist of erythritol. We revealed that thin-layer sugarless coated tablets have both advantages of film-coated tablets and sugar-coated tablets.     

复方金连冲剂薄层色谱鉴别

复方金连冲剂系由黄连、桅子、厚朴等数昧中药制成的复方中药制剂，具有清热化湿、和胃消导、调畅气机之功效，用于肠胃湿热及慢性胃肠炎等症。本文应用TLC法对方中黄连、柜子、厚朴进行鉴别。习材料与试剂复方金连冲剂（本院制剂室，批号950502、950915、960625）；阴性对照液，取处方药味除去被测药材，其余药味按复方金连冲剂的制备方法及供试品溶液的制备方法制备；盐酸小经碱、柜子式、厚朴酚、和厚朴酚对照品、黄连对照药材（。11国药品生物制品检定所入硅胶G（浙江雁荡山试剂厂）；沈胶C风。（青岛海洋化工厂），所用试剂均为分析…     

I. Isolation and characterization of the transformation products of maridomycin III.

Three transformation products of maridomycin (MDM) III, a macrolide antibiotic, by Streptomyces lavendulae were isolated by silica gel and alumina chromatography, and designated as spots 1 (starting MDM III), 2, 3 and 4, in the order of their decreasing Rf values on thin-layer chromatogram. By mass- and NMR-spectrometry and thin-layer chromatography, spot 2 was identified as 18-dihydro-MDM III, spot 3 as 4'-depropionyl-MDM III, and spot 4 as 18-dihydro-4'-depropionyl-MDM III. The relationship between starting MDM III and these transformation products were also discussed.     

薄层色谱法测定地红霉素中的有关物质

目的采用薄层色谱法检查地红霉素中的有关物质。方法地红霉素供试品经无水甲醇溶解后 ,以正己烷 二氯甲烷 甲醇 浓氨水 (2∶3∶0 .8∶0 .0 6)为展开剂 ,在硅胶GF2 5 4薄层板上展开 ,以硫酸显色后观察。结果确立供试品有关物质限度。结论此法简便、灵敏、准确 ,可作为地红霉素有关物质的快速测定     

Chiral separation by chromatographic and electromigration techniques. A review

This review gives a survey of different chiral separation principles and their use in high-performance liquid chromatography (HPLC), gas chromatography (GC), supercritical fluid chromatography (SFC), thin-layer chromatography (TLC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) highlighting new developments and innovative techniques. The mechanisms of the different separation principles are briefly discussed and some selected applications are shown.     

Assay and purity control of metacycline by thin-layer chromatography combined with UV and fluorescence densitometry--a comparison with liquid chromatography

A thin-layer chromatographic (TLC) method involving UV and fluorescence densitometry is described for the assay and purity control of metacycline. With a mobile phase dichloromethane-methanol-water (58:35:7, v/v/v) and a silica gel thin-layer, previously sprayed with 10% sodium edetate solution adjusted to pH 9.0, all the potential impurities of metacycline were well separated from the main component and from each other. Results obtained with UV densitometry (TLC-UV) and fluorescence densitometry (TLC-F) were compared with those obtained by a liquid chromatography (LC) method using a poly(styrene-divinylbenzene) stationary phase. The correlation coefficients (r) for TLC-UV and LC or TLC-F and LC were better than 0.9999. For TLC-UV the relative standard deviation (RSD) for the assay of the main component was less than 2%, for TLC-F less than 3.0% and for LC less than 1.0%.