中国延边朝鲜族和汉族D17S30非翻译区的可变数目串联重复序列多态性研究

[目的 ]探讨中国延边朝鲜族和汉族D17S3 0位点的非翻译区的可变数目串联重复序列 .[方法 ]应用聚合酶链反应法 .[结果 ]中国延边朝鲜族和汉族等位基因频率总体分布之间均有显著性差异 ;D17S3 0位点的非翻译区的可变数目串联重复序列在中国延边朝鲜族和汉族中的观察杂合性、多态信息含量、个体鉴别力均高于 70 % ;中国延边朝鲜族和汉族中D17S3 0非翻译区的可变数目串联重复序列位点频率分别以A3和A1的为最高 .[结论 ]D17S3 0非翻译区的可变数目串联重复序列在中国延边朝鲜族和汉族中的基因频率分布具有很强的多态性 .     

牡丹江市朝鲜族人群15个常染色体STR基因座的频率调查

目的：调查牡丹江市朝鲜族人群无关个体的15个常染色体STR基因座（D2S1338、D18S51、D7S820、D8S1179、D5S818、D13S317、D16S539、vWA、D19S433、TPOX、D3S1358、FGA、CSF1PO、D21S11、TH01）的多态性,研究其在法医学亲子鉴定和个人识别检验中的实际应用价值。方法：应用AmpFL STR IdentifilerTM试剂盒扩增牡丹江市500例朝鲜族无血缘关系个体的15个STR基因座,其产物用ABI 310遗传分析仪进行检测,包括电泳分离和分型检测,分析其基因频率和遗传平衡分析,并计算分析其遗传参数：杂合度（H）、多态性信息含量（PIC）、个人识别能力（DP）。结果：500名无血缘关系个体各基因座基因型分布均符合Hardy-Weinberg平衡（P〉0.05）。15个STR基因座的H都大于0.6,DP都大于0.8,PIC都大于0.5。结论：15个STR基因座在牡丹江市朝鲜族人群中等位基因分布较好,具有较高的遗传多态性,所得的群体遗传学数据可满足该地区人类遗传学、法医学亲子鉴定和个人识别等领域的研究需要。     

中国河北汉族人群4个STR位点遗传多态性

目的：检测中国河北省汉族人群D2S1328、D3S1358、D11S2010、D16S310 4个STR位点的群体遗传学数据，探究其在法医学应用中的意义。方法：从河北省无血缘关系汉族个体的抗凝血中提取DNA，进行PCR扩增，变性聚丙烯酰胺凝胶垂直电泳分型，银染显色。结果：4个位点基因频率分布均符合Hardy-weinberg平衡，遗传均符合孟德尔遗传规律，呈共显性遗传。4个基因座联合个人识别率（DP）为0.9995，累计多态信息量（PIC）为0.9807。结论：这4个多态位点在中国河北汉族人群中具有良好的多态性，所得到的遗传学数据可用于河北汉族人群法医学个人识别和亲子鉴定及遗传学研究。     

朝鲜族DXS6797STR位点的遗传多态性分析

[目的]研究延边地区朝鲜族人群短串联重复序列(STR)基因座DXS 6797的遗传多态性,获得该基因位点的群体遗传学数据.[方法]应用PCR扩增技术及聚丙烯酰胺凝胶电泳方法,对106名朝鲜族女性个体和186名朝鲜族男性个体的DXS 6797 STR基因座等位基因频率进行分析.[结果]在女性样本DXS 6797基因座中各检出7种等位基因和16种不同的基因型,分析基因型观察值和期望值,基因座多态性分布符合Hardy-Weinberg平衡定律,杂合度为0.717,多态信息含量为0.68,个人识别率为0.876;在男性样本DXS 6797基因座中共检出7种等位基因,单倍型多样性为0.745 5.[结论]STR基因座DXS 6797具有较高的个人识别力,可应用于法医学检验和朝鲜族人群遗传学分析.     

D1S80基因座在朝鲜族和汉族人群中的遗传多态性

[目的]研究VNTR基因座D1S80在朝鲜族和汉族群体中的遗传多态性.[方法]采用PCR扩增结合聚丙烯酰胺凝胶电泳技术和银染法对207名无关朝鲜族个体和203名无关汉族个体的D1S80基因座进行群体遗传多态性调查.[结果]D1S80基因座在朝鲜族和汉族人群中共检出26个等位基因,共103种基因型,朝鲜族群体中检出25个等位基因,73种基因型;汉族群体中检出22个等位基因,71种基因型;两个群体皆符合Hardy-Weinberg平衡,朝鲜族群体的杂合度观察值、多态性信息量、非父排除率和个人识别能力分别为0.917 9,0.893 8,0.705 9,0.968 3,汉族群体中分别为0.803 0,0.870 3,0.684 2,0.968 8;将两个群体的等位基因分布频率分别与其他不同地区、民族、种族群体进行比较,结果证实D1S80基因座在不同地区、民族和种族之间存在着不同程度的差异.[结论]D1S80基因座在朝鲜族和汉族群体中具有高度多态性,可用于法医学个体识别、亲权鉴定和群体遗传学调查.     

西藏藏族15个STRs位点多态性及其与其他民族群体的遗传关系

目的:研究西藏藏族人群15个短串联重复序列(short tandem repeats,STRs)位点(D8S1179,D21S11,D7S820, CSF1P0,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818,FGA)的多态性分布及群体遗传学和法医学应用价值.并分析它们与西藏其他民族及其他亚洲人群间的遗传学关系.方法:采用AB13100遗传分析仪检测STRs基因多态性,用ARLEQUIN 3.1软件计算等位基因频率和各种多态性参数.并将其结果与文献报道的其他亚洲人群的STRs结果进行比对,DISPAN软件计算遗传距离(DA)、基因分化系数(Gst)和杂合度(Ht),MEGA4.0软件绘制进化树,SPSS14.0进行多维量表法(MDS)分析.结果:藏族群体中共检出132种等位基因,频率分布0.0050～0.5990;杂合度、个体识别力、多态性信息量等群体遗传学指标分析显示,15个STRs位点具有中度或高度多态性,中国藏族群体具有较独立的遗传结构.结论:所选择的15个STRs位点具有较高的个体识别力和多态性信息量,可用于群体遗传学和法医学研究.     

延边朝鲜族人群Ⅰ型胶原蛋白A2基因MspI多态性位点研究

[目的 ]了解中国朝鲜族人群的Ⅰ型胶原蛋白A 2基因MspI多态性位点的存在和等位基因频率 ,并与其它地区人群进行比较 .[方法 ]按酚 氯抽提法提取人类基因组DNA ,采用聚合酶链反应 限制性片段长度多态性方法对Ⅰ型胶原蛋白A 2基因MspI多态性位点进行检测 .[结果 ]12 0名个体中 ,无酶切位点的纯合个体为 89例 ,P-P-基因型频率为 74 2 % ;有酶切位点的杂合个体为 2 2例 ,P+ P-基因型频率为 18 3% ;有酶切位点的纯合个体为 9例 ,P+ P+ 基因型频率为 7 5 % ;2 4 0个等位基因中MspI酶切位点基因P+ 的频率为 16 7% .[结论 ]延边朝鲜族人群Ⅰ型胶原蛋白A 2基因存在多态性 ,Ⅰ型胶原蛋白A 2基因MspI多态性分布与其它地区人群的分布有所不同 ,可以作为朝鲜族的一个遗传标记 .     

朝鲜族人群DXS6854基因座的遗传多态性研究

[目的]探讨吉林省延边地区朝鲜族人群短串联重复序列(STR)基因座DXS 6854的遗传多态性分布,获取相应多态位点的群体遗传学数据.[方法]采用PCR扩增技术及聚丙烯酰胺凝胶电泳方法,分析101名朝鲜族女性和160名朝鲜族男性个体的DXS 6854 STR基因座等位基因频率.[结果]在女性样本DXS 6854基因座中各检测出6种等位基因和17种不同的基因型,基因座多态性分布符合Hardy-Weinberg平衡定律,杂合度为0.772 3,多态信息含量为0.67,个人识别率为0.857;在男性样本DXS 6854基因座中检测出6种等位基因,单倍型多样性为0.734.[结论]DXS 6854基因座具有较高的个人识别力、杂合度及多态信息量,是较理想的遗传标记系统,对朝鲜族群体的个人识别、亲子鉴定及民族遗传学研究具有重要意义.     

深圳地区汉族群体9个STR位点的遗传多态性调查

目的 :了解深圳汉族人群 9个 STR位点各等位基因的频率分布 ,获得汉族群体的遗传数据。方法 :采用 Amp Fl STR Profiler Plus试剂盒 ,测序胶电泳分离 ,结合 ABIPrism377自动荧光测序仪对深圳汉族人群 D3S1 35 8、v WA、FGA、D8S1 1 79、D2 1 S1 1、D1 8S5 1、D5 S81 8、D1 3S31 7和 D7S82 0 9个 STR位点遗传多态性进行分析。结果 :9个 STR位点累积个人识别率分为 0 .99999999983和累积单亲父子非父排除率 0 .9930 ,累积父母子非父排除率为 0 .99973。结论 :9个 STR位均具有遗传多态性 ,具有较高的个体识别能力 ,适用于法医学个体识别及亲子鉴定 ,所得数据为群体遗传学研究和法医学应用提供了依据     

吉林地区汉族群体两个STR基因座的遗传多态性调查

目的 分析吉林地区汉族人群两个短串联重复序列（STR）位点即D5S818和D19S400的遗传多态性分布,获得群体遗传学数据。方法 从吉林地区103名汉族无血缘关系的个体抗凝血样中提取DNA,进行PCR扩增,非变性聚丙烯酰胺凝胶电泳分型并银染。结果 得到两个基因座各等位基因的基因频率,基因型频率分布符合Hardy-Weinberg平衡。杂合度和多态性信息含量较大。结论 这两个基因座具有高度多态性,是较理想的遗传标记。所得到的遗传学数据可为法医学个人识别、亲子鉴定及群体遗传学研究提供依据。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.