高能震波对狗睾丸的近期影响

用JDPN—Ⅳ型体外震波碎石机对20条狗的输尿管下段进行冲击,于冲击后第1、3、7、14、28天分别取睾丸组织作病理学检查及超微结构检查,以了解高能震波对睾丸组织的影响。结果发现经高能震波冲击后肉眼可见狗的附睾和睾丸被膜出血:光镜下可见睾丸组织毛细血管扩张、瘀血、毛细血管出血、成熟的生精细胞排列紊乱、Johnsen积分下降,电镜下可见生精细胞线粒体肿胀、嵴变平或消失、粗面内质网脱颗粒。     

内蒙古地区成人不典型淋巴细胞和不成熟细胞百分比参考范围调查

目的：探讨内蒙古地区汉族健康成人不典型淋巴细胞、不成熟细胞百分比的参考范围。方法：按说明操作BEKMAN-COULTER AC.T5diff血细胞仪,进行702例标本检测,测定不典型淋巴细胞、不成熟细胞百分比数值,并以x-±1.96s计算各项指标参考值范围。结果：BEKMAN-COULTER AC.T5diff所研究的内蒙古地区汉族健康成人不典型淋巴细胞、不成熟细胞百分比的参考范围分别为0%-3.25%、0%-3.65%。结论：内蒙古地区不典型淋巴细胞、不成熟细胞百分比男女性别组比较差异均无统计学意义（P〉0.05）,应建立正常人群不典型淋巴细胞、不成熟细胞百分比各项参数参考值。     

大鼠胚胎小脑组织移植的研究 Ⅰ.大鼠胚胎小脑组织移植的形态学观察

取孕17～20天的Wistar大鼠胚胎小脑组织植入同种大鼠小脑内。移植后10天,移植块与宿主脑组织融为一体,两者之间无隔膜,也无疤痕组织;移植物明显变大,充满预制脑腔,其外颗粒层细胞分裂相多,普肯野细胞结构特征出现,分子层、普肯野细胞层及内颗粒层依次排列,小脑皮质形成。移植后20天,普肯野细胞明显排成一层,尼氏体出现。移植后30天,外颗粒层消失,小脑皮质成熟。实验证明,同种胚胎脑组织移植,移植块可成活并按其固有模式生长成熟。     

人半规管的胚胎发育及胎儿壶腹嵴暗细胞的超微结构观察

收集10～37周的胎儿58例,用光镜观察半规管的发育,用透射电镜观察其中11例壶腹嵴暗细胞的发育。结果显示(1)膜半规管壁在胚胎10周时由单层立方上皮及很薄的基膜组成,14周时该壁由3层结构组成,20周时已成熟;外淋巴间隙在胚胎10周时即形成,16周时接近成熟;半规管的骨化始于胚胎15周,20周时骨半规管形成。(2)壶腹嵴暗细胞在胚胎10周时即具有吸收细胞的特征并参与耳石的代谢,17周时其形态已成熟;壶腹嵴暗细胞区的黑色     

钛合金颗粒存在下破骨细胞的体外诱导培养

目的 探讨钛合金颗粒存在下小鼠巨噬细胞体外诱导培养为成熟破骨细胞的方法。方法 获取小鼠骨髓巨噬细胞,采用巨噬细胞集落刺激因子依赖性前体细胞诱导法,于钛合金Ti-6Al-4V颗粒存在条件下进行诱导培养,培养后细胞行抗酒石酸酸性磷酸酶（TRAP）染色,RT-PCR法检测破骨细胞特异性TRAP、CK、CR mRNA表达;采用相同方法于骨片上诱导培养细胞并观察骨吸收陷窝。结果 Ti-6Al-4V颗粒存在下培养第6天细胞开始出现融合,第9天出现多核巨细胞,TRAP染色阳性,细胞内有破骨细胞特异性TRAP、CK、CR mRNA表达;于骨片上诱导培养的细胞可形成骨吸收陷窝。结论 钛合金颗粒存在下,成功将小鼠骨髓巨噬细胞体外诱导培养为成熟破骨细胞。     

HL-60细胞在诱导分化中的超微结构及细胞化学研究

用电镜及超微结构细胞化学方法,观察并比较了RA及DMSO诱导HL-60细胞分化的演变过程,两种诱导剂均可使HL-60细胞分化为有吞噬功能的粒细胞,但DMSO处理组尚出现少量分化为单核细胞。超微结构和细胞化学的结果说明RA诱导HL-60细胞分化有一个清晰的成熟演变过程。在诱导剂处理四天中,从最初表现为核变形至分裂、胞浆A颗粒由疏松变为致密以及S颗粒和吞噬活动出现;MPO反应从开始时出现于内质网、核膜腔、A颗粒,演变为最终只存在于A颗粒。DMSO处理细胞在第二、三天间有一个诱导分化的转折点。对上述结果进行了初步讨论、     

钛合金颗粒存在下破骨细胞的体外诱导培养

目的 探讨钛合金颗粒存在下小鼠巨噬细胞体外诱导培养为成熟破骨细胞的方法.方法 获取小鼠骨髓巨噬细胞,采用巨噬细胞集落刺激因子依赖性前体细胞诱导法,于钛合金Ti-6Al-4V颗粒存在条件下进行诱导培养,培养后细胞行抗酒石酸酸性磷酸酶（TRAP）染色,RT-PCR法检测破骨细胞特异性TRAP、CK、CR mRNA表达;采用相同方法于骨片上诱导培养细胞并观察骨吸收陷窝.结果 Ti-6Al-4V颗粒存在下培养第6天细胞开始出现融合,第9天出现多核巨细胞,TRAP染色阳性,细胞内有破骨细胞特异性TRAP、CK、CR mRNA表达;于骨片上诱导培养的细胞可形成骨吸收陷窝.结论 钛合金颗粒存在下,成功将小鼠骨髓巨噬细胞体外诱导培养为成熟破骨细胞.     

短期糖皮质激素治疗对新生儿肾上腺皮质束状带影响的电镜观察

本文对9例足月新生儿死亡的新鲜肾上腺组织进行了电镜检查。根据激素应用与否分为激素组5例,对照组4例,观察短期(3～6天)大剂量激素(地塞米松0.25～0.5 mg/kg/天)治疗对新生儿肾上腺皮质束状带细胞超微结构的影响。电镜下示激素组肾上腺皮质束状带外层细胞线粒体含嵴数量减少,出现大量管状嵴,可见退变的巨大线粒体:滑面内质网平滑膜管减少,有些则呈串孢状扩张,腔内含有紫状物:类脂滴体积扩大,密度坛加。上述改变说明即使是短期应用大剂量激素治疗亦可致新生儿HPA轴抑制,肾上腺皮质功能减退。     

迷走神经切断术影响大鼠胸腺细胞亚型分布的实验研究

目的 探讨迷走神经在胸腺细胞成熟发育和输出过程中的作用.方法 实验采取颈部右侧迷走神经切断术,应用免疫组织化学和流式细胞术检测大鼠胸腺CD4、CD8阳性细胞表达及分布变化情况.结果 CD4、CD8阳性细胞分布于大鼠胸腺特定区域,单侧迷走神经切断后阳性细胞分布面积减小,未成熟胸腺细胞百分比降低,成熟胸腺细胞百分比升高.结论 迷走神经切断术后,胸腺中未成熟T细胞减少,成熟T细胞数量相对增多.迷走神经在T细胞发育及输出过程中起重要作用.     

衰老大鼠垂体前叶超微结构的定量分析——(Ⅱ)参与激素合成与分泌的细胞器年龄性变化

在对衰老动物垂体各内分泌细胞的胞核、核仁、线粒体及脂褐素和核质比进行立体定量分析研究的基础上,又对其激素合成与分泌的亚细胞结构进行研究。发现衰老时垂体各种内分泌细胞中粗面内质网、高尔基复合体和内分泌颗粒的Vv、Sv和Nv都有不同程度的增加或减少。在ACTH、LH/FSH细胞和TSH细胞中,粗面内质网的Vv、Sv随年龄的增长而减少,其中,LH/FSH细胞的高尔基复合体Vv、Sv和ACTH细胞的高尔基复合体Sv也同样出现年龄性降低.此外,GH细胞成熟或未成熟分泌颗粒Vv、Nv随年龄增长而有所增加;在PRL细胞中,成熟分泌颗粒Vv、Nv表现为增龄性增加,而未成熟分泌颗粒的Vv、Nv却反而减少。TSH细胞成熟分泌颗粒Vv增加,而未成熟分泌颗粒Nv和LH/FSH细胞的未成熟颗粒Nv却随年龄增长而减少.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.