Effect of moxibustion on survival status and nutritional metabolic factor in tumor-bearing rats with gastric cancer

Objective:To observe the effect of moxibustion on the survival status and nutritional metabolic factors of tumor-bearing rats with gastric cancer and explore the inhibitory effect of moxibustion on the tumorbearing rats.Methods:A total of 40 SD rats were randomly divided into a blank group,a sham-operated group,a model group and a moxibustion group,10 rats in each group.Gastric cancer models were established in the model group and the moxibustion group.The rats of all the groups underwent the same constraining procedure for 20 min every day.Additionally,moxibustion was applied at acupoints for 20 min in the rats of the moxibustion group.There were two groups of acupoints.One group included"Zusanli(足三里ST 36)""Zhongwan(中脘CV12)"and"Guanyuan(关元CV4)".The other group included bilateral"Pishu(脾俞BL 20)"and"Weishu(胃俞BL 21)".Moxibustion was applied for 20 min every day at the acupoints of the two groups alternatively and lasted for 14 days.The survival status of rats was observed and the score of survival status and body weight of rats were recorded every day.At the end of intervention,the orbital blood was collected and the rats were sacrificed for sample collection.The viscera-free body weight was recorded.Using biochemical analyzer,the blood glucose(GLU),albumin(ALB),total protein(TP)and triglyceride(TG)in serum were detected.Results:(1)Regarding the score of the survival status,after modeling,the scores in the model group and the moxibustion group were all higher than those of the sham-operation group(both P0.05).After intervention,the score in the moxibustion group was lower than that of the model group(P0.05).(2)After modeling,the body weight in the moxibustion group and the model group was lower than those of the blank group,indicating a statistical significance(both P0.05).The body weight in the moxibustion group was higher than that of the model group after intervention,indicating the statistical significance(P0.05).(3)Regarding the viscera-free body weight,it was lower in the model group compared with the sham-operation group,indicating the statistical significance(P0.05).Compared with the model group,the viscera-free body weight was higher in the moxibustion group,indicating the statistical significance(P0.04).(4)Regarding the nutritional metabolic factors,compared with the sham-operation group,the levels of GLU and ALB in the model group were lower and TP was higher,presenting the statistical significance(all P0.05).Compared with the model group,the levels of GLU and ALB were higher in the moxibustion group and TP was lower,presenting the statistical significance(all P0.05).Conclusion:Moxibustion improves the survival status of tumor-bearing rats with gastric cancer,increases the body weight,the viscera-free body weight and the levels of GLU and ALB in serum and reduces TP of the tumor-bearing rats with gastric cancer and inhibits the growth of gastric cancer.     

脊髓横断后艾灸足三里对胃黏膜损伤大鼠Nrf2/HO-1信号通路的影响

目的： 观察艾灸足三里通过调节Nrf2/HO-1信号通路对应激性胃黏膜损伤大鼠的治疗作用,并探讨其胃黏膜保护作用与脊髓的关系。方法：SPF级SD大鼠50只,按体质量随机分为空白组、模型组、药物组、艾灸组和手术+艾灸组,每组各10只。采用无水乙醇结合阿司匹林混悬液灌胃建立大鼠胃黏膜损伤模型,艾灸组艾灸大鼠双侧“足三里”穴,药物组给予奥美拉唑肠溶片溶液(0.2 mg/kg)灌胃,连续治疗8 d;手术+艾灸组予以脊髓横断手术,术后进行胃黏膜损伤造模及艾灸治疗。治疗结束后,应用Guth法计算大鼠胃黏膜损伤指数(UI),HE染色观察大鼠胃黏膜形态学变化,ELISA实验检测血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)和谷胱甘肽还原酶(GSH-Px)的水平变化。Western-blot和RT-PCR检测胃黏膜组织中一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)、核转录因子E2相关因子2(Nrf2)和血红素加氧酶-1(HO-1)的蛋白及mRNA表达。结果：与模型组相比,艾灸组大鼠UI值显著降低(P＜0.01),胃黏膜组织损伤明显改善,明显降低了血清中MDA、NO水平,升高了SOD、GSH-Px的水平(P＜0.01)。显著下调了胃黏膜组织中iNOS和COX-2的蛋白及mRNA表达水平,上调了Nrf2和HO-1的表达水平(P＜0.01)。手术+艾灸组大鼠UI值、血清中MDA、NO水平及胃黏膜组织中iNOS和COX-2的表达较艾灸组升高,血清SOD、GSH-Px的水平及黏膜组织Nrf2和HO-1的表达水平明显降低,差异均具有显著性意义(P＜0.05)。结论：艾灸足三里可通过Nrf2/HO-1信号通路有效改善大鼠应激性胃黏膜损伤,脊髓参与了其修复作用及信号传导的过程。     

EFFECTS OF MOXIBUSTION ON BEHAVIORAL CHANGES AND BRAIN OXIDATIVE STATE IN APOLIPOPROTEIN E-DEFICIENT MICE

Objective: To determine whether moxibustion influences the learning and memory ability of the apolipoprotein E-deficient(ApoE-/-) male mice by altering oxidative stress markers(superoxide dismutase(SOD) and malondialdehyde(MDA)) and oxidized proteins(glial fibrillary acidic protein(GFAP) and mortalin) in the hippocampus.Methods: Thirtheen wild-type C57BL/6 normal mice were allocated into the control group while 33 ApoE-/- mice were randomly divided into 3 groups(n=11): model group, shammoxibustion group, and real moxibustion group.Mice of the real moxibustion group received 20 min of moxibustion 6 d per week for 12 weeks on acupoint Shenque(RN8).In the sham control group, the moxa stick was not burned.The step-down test and open-field test were conducted during the 13 th week.Brain GFAP and mortalin levels were evaluated using immunohistochemicalstaining.Levels of MDA and activity of SOD were determined by colorimetric method.Results: In the step-down test, in the aspect of learning record, the moxibustion group(5.82±5.30; 0.33±0.50) demonstrated shorter reaction time(P0.05) and committed less wrong times(P0.05) than the sham-moxibustion group(67.68±87.36; 2.17±1.94).In the open-field test, there was no significant difference between the moxibustion group and the sham control.Moreover, SOD activity was increased(P0.05) and MDA level was significantly decreased(P0.05) in the moxibustion group(104.68±15.06; 6.30±1.43) compared with that in the sham-control group(84.85±5.44; 8.01±1.20).Both the sham-moxibustion group and the moxibustion group had similar GFAP level in the hippocampus.There was a decreasing tendency of mortalin level in the real moxibustion group compared with the sham moxibustion group.Conclusion: Our findings suggest that moxibustion may enhance learning capability and tend to have an effect on cognitive abilities of ApoE-/- mice by scavenging oxygen free radicals, stimulating antioxidant enzyme activity and reducing oxidized proteins in the brain.Further studies are needed to investigate the effect of moxibustion on other brain oxidized protein.     

Effects of moxibustion and acupuncture at Zusanli(ST 36) and Zhongwan(CV 12) on chronic atrophic gastritis in rats

OBJECTIVE: To evaluate the effects of moxibustion and acupuncture of Zusanli(ST 36) and Zhongwan(CV 12) acupoints on chronic atrophic gastritis(CAG) in rats, and to study the mechanisms behind their actions.METHODS: Forty-four male Sprague-Dawley rats were induced with CAG by intragastric administration of 40% ethanol combined with free drinking of N-methyl-N′-nitro-N-nitrosoguanidine and irregular feeding for 12 weeks, followed by daily treatment with moxibustion or acupuncture for 2 weeks. Histopathologic examination, Western blotting of cytokines [epidermal growth factor(EGF),EGF receptor(EGFR), extracellular signal-regulated kinase(ERK), phosphorylated ERK(p-ERK)], and1 H NMR-based metabolic profiling of gastric tissues were used to measure changes related to CAG modeling and treatment.RESULTS: Moxibustion and acupuncture at Zusanli(ST 36) and Zhongwan(CV 12) each relieved CAG-induced abnormalities in histopathology and cytokine expression of ERK and p-ERK. Only moxibustion treatment regulated the expression of EGF and EGFR. Metabolites that were increased in gastric tissue by CAG induction(alanine, nicotinamide adenine dinucleotide phosphate, uracil DNA glycosylase, lactate, glycerol and adenosine) were restored to normal levels after moxibustion treatment; acupuncture treatment only normalized the levels of adenosine monophosphate and glycerol.CONCLUSION: Our findings suggest that moxibustion or acupuncture at Zusanli(ST 36) and Zhongwan(CV 12) can significantly improve the condition of CAG in rats. These treatments exert their effects on CAG through different mechanisms.     

EFFECTS OF ACUPUNCTURE AT DIFFERENT ACUPOINTS ON EXPRESSION OF SERUM IFN-γ AND HYPOTHALAMIC β-EP mRNA IN RATS AFTER IMMUNOSUPPRESSION

Objective: To compare the effects of acupuncture at different acupoints on the content of serum IFN-γ and expression of hypothalamic β-EP mRNA in rats after immunosuppression, and to investigate the mechanism of acupuncture in regulating immune function.Methods: Healthy male SD rats were randomly divided into normal control group, immunosuppressive group, Baihui group, Zusanli group, Baihui and Zusanli group, 8 rats in each group.Immunosuppression model was made by cyclophosphamide injection, and acupuncture was performed at Baihui(DU20) and/or Zusanli(ST36).Seven days after acupuncture, enzyme-linked immunosorbent assay(ELISA) was used to detect the content of serum IFN-γ, and real-time quantitative PCR(RT-PCR) was used to detect the expression of β-EP mRNA in all rats.Results: Compared with the normal control group, the levelof serum IFN-γ decreased significantly in the immunosuppressive group(P0.01).Compared with the immunosuppressive group,the content of serum IFN-γ in the Baihui group, the Zusanli group, and the Baihui and Zusanli group was improved significantly, and the Baihui and Zusanli group was better than the other two groups(P0.05).Compared with the normal control group, the expression of hypothalamus β-EP mRNA was decreased significantly(P0.01).Compared with the immunosuppressive group, the expression of hypothalamic β-EP mRNA in the Baihui group, the Zusanli group, and the Baihui and Zusanli group was promoted significantly, and the Baihui and Zusanli group was better than the other two groups(P0.05).Conclusion: The content of serum IFN-γ and expression of hypothalamic β-EP mRNA were increased in rats after immunosuppression by acupuncturing Baihui(DU20) and Zusanli(ST36), which may be one of the mechanisms of enhancing the immune function.     

艾灸预处理对应激性胃黏膜损伤大鼠血清IL-1p、TNF-α和IL-10的影响

目的 观察艾灸预处理对应激性胃黏膜损伤大鼠白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和白细胞介素-10(IL-10)的影响,探讨艾灸预处理抗胃黏膜炎症损伤的作用机制.方法 将SD大鼠随机分为空白组、模型组、艾灸穴位组、艾灸非穴点对照组.束缚冷应激法制作应激性胃黏膜损伤模型,按Guth法计算胃黏膜损伤指数(UI),放射免疫法测定血清IL-1β、TNF-α和IL-10的含量.结果 与模型组和艾灸非穴点对照组比较,艾灸足三里、中脘等穴可使应激性胃黏膜损伤UI明显下降、血清IL-1β含量降低、TNF-α含量降低、IL-10含量升高.结论 艾灸足三里、中脘等穴预处理可促进大鼠胃黏膜损伤的修复、减轻急性炎症反应,可能是通过抑制细胞炎症反应的免疫促炎因子IL-1β、TNF-α和促进抗炎因子IL-10而达到其抗胃黏膜损伤作用.     

Effects of Moxibustion Pretreating on SOD and MDA in the Rat of Global Brain Ischemia

Objective： To probe into the mechanism of moxibustion preconditioning in preventive brain-protecting effect. Methods： The global brain ischemia rat model was developed by blocking 4 arteries. Seventy-eight Wistar male rats were randomly divided into 5 groups： a normal control group, a sham-operation group, a brain ischemia group, a brain ischemia preconditioning group, a moxibustion pretreating group. The brains in the 5 groups were taken at 24 h, 48 h, and 72 h after operation respectively. Superoxide dismulase （SOD） activity was determined with xanthine oxidase method and malondialdehyde （MDA） content with thiobarbituric acid method. Results： After the operation, in the moxibustion preconditioning group, SOD activity significantly increased, especially 24 h after moxibustion preconditioning; and MDA content decreased, with a very significant difference as compared with that of the cerebral ischemia group （P〈0.01）. Conclusion： Moxibustion preconditioning protects the ischemic and anoxic brain tissue by increasing the activity of endogenous antioxidase.     

艾灸对大鼠应激性胃黏膜损伤修复及p-ERK1/2蛋白表达的影响

目的:探讨艾灸促进大鼠应激性胃黏膜损伤修复作用及细胞机制。方法:SD大鼠48只随机分为空白组、模型组、艾灸穴位组、艾灸非穴组,每组12只。束缚冷应激法制作应激性胃黏膜损伤大鼠模型,在造模之前,艾灸穴位组选取足三里、中脘、脾俞、胃俞等穴位行艾灸预处理8d。按Guth法计算胃黏膜损伤指数(UI),免疫组化、蛋白质印迹法(Western Blot)检测胃黏膜磷酸化细胞外信号调节激酶1/2(phosphorylating extracellularsignal-regulated kinase1/2,p-ERK1/2)蛋白表达。结果:与空白组比较,模型组大鼠UI值显著增高(P0.01)、胃黏膜细胞p-ERK1/2蛋白表达加强(P0.05,P0.01);与模型组、艾灸非穴组比较,艾灸穴位组大鼠UI值明显下降(P0.01)、胃黏膜细胞p-ERK1/2蛋白表达增强(P0.01)。结论:艾灸足三里、中脘等穴位预处理可促进束缚水浸应激所造成大鼠胃黏膜损伤的修复,其作用机制可能与上调胃黏膜p-ERK1/2蛋白表达有关。     

艾灸预处理对大鼠应激性胃黏膜损伤的保护作用

目的:探讨艾灸预处理对大鼠应激性胃黏膜损伤保护作用及抗炎症损伤机制。方法:将48只健康SD大鼠随机分为4组,即空白组、模型组、艾灸穴位组、艾灸非穴组。束缚冷应激法制作应激性胃黏膜损伤大鼠模型,按Guth法计算胃黏膜损伤指数(UI),光镜下观察大鼠胃黏膜组织学改变,放射免疫法测定血清IL-1β,TNF-α和IL-10的含量。结果:与艾灸非穴组比较,艾灸足三里、中脘等穴位可使应激性胃黏膜损伤大鼠UI明显下降(P0.01)、血清IL-1β含量降低(P0.05)、TNF-α含量降低(P0.01)、IL-10含量升高(P0.05)。结论:艾灸足三里、中脘等穴位预处理可促进束缚水浸应激所造成大鼠胃黏膜损伤的修复、减轻急性炎症反应,该保护作用可能是通过抑制细胞炎症反应的免疫促炎因子IL-1β、TNF-α和促进抗炎因子IL-10而达到其抗胃黏膜损伤作用。     

Research on the treatment of ulcerative colitis （UC） guided by the mechanism of moxibustion

Objective To investigate the efficacy and the mechanism of moxibustion in ulcerative colitis(UC) rats model. Methods Forty general clean-class health Wistar rats, were randomly divided into four groups(each with 10 rats), namely the normal group, the blank group, moxibustion group and the control groups.     

Effect and Mechanism of Moxibustion on Acupoints Zusanli （ST 36） and Liangmen （ST 21） in the Prevention and Treatment of Stress Ulcer

目的观察艾灸足三里等穴对应激性溃疡大鼠胃粘膜细胞增殖及凋亡的影响,探讨艾灸促进胃粘膜损伤修复的细胞分子生物学机制.方法60只SD大鼠随机分为4组,即束缚对照组、模型组、艾灸足三里和梁门穴组和艾灸非穴对照点组.束缚水浸应激法制备胃溃疡模型,放射免疫方法测定胃粘膜转化生长因子(TGF-α)的含量,免疫组织化学方法检测HSP70蛋白,胃粘膜增殖细胞核抗原(PCNA)及细胞凋亡指数.结果束缚水浸应激法造模后可致胃粘膜损伤指数升高,胃粘膜TGF-α含量下降;PCNA下降,HSP70蛋白增加;细胞凋亡指数增加(P＜0.01).艾灸足三里和梁门可降低胃粘膜损伤指数,增加胃粘膜TGF-α含量,促进HSP70和PCNA的表达,降低胃粘膜细胞凋亡指数,与模型组和对照组比较有显著性差异(P＜0.01).结论艾灸足三里和梁门对应激性溃疡胃粘膜具有保护作用,机制可能是促进TGF-α合成、刺激胃粘膜细胞增殖、抑制细胞凋亡,而这一过程与艾灸诱导HSP70表达有关.     

艾灸对应激性胃溃疡大鼠胃粘膜细胞增殖和凋亡的影响及其与热休克蛋白表达关系的研究

目的:观察艾灸“足三里”等穴对应激性胃溃疡大鼠胃粘膜细胞增殖及凋亡的影响,分析热休克蛋白70基因表达(HSP70 mRNA)与上述效应的关系,探讨艾灸促进胃粘膜损伤修复的细胞分子生物学机制。方法:60只健康SD大鼠随机分为4组,即束缚对照组、模型组、艾灸“足三里”-“梁门”穴组和艾灸非穴对照点组。束缚水浸应激法制备胃溃疡模型,采用放射免疫方法测定胃粘膜转化生长因子(TGFα-)的含量,RT-PCR法测定HSP70 mRNA,免疫组织化学方法检测胃粘膜增殖细胞核抗原(PCNA)及细胞凋亡指数。结果:束缚水浸应激法造模后可致胃粘膜损伤指数升高,胃粘膜TGFα-含量下降,PCNA下降,细胞凋亡指数、HSP70 mRNA增加(P<0.05或P<0.01)。艾灸“足三里”“梁门”可降低胃粘膜损伤指数,增加胃粘膜TGFα-含量,促进HSP70 mRNA和PCNA的表达,降低胃粘膜细胞凋亡指数,与模型组和对照组比较有显著性差异(P<0.05或P<0.01)。结论:艾灸“足三里”“梁门”对应激性胃溃疡的胃粘膜具有保护作用,其机制可能是促进了TGFα-合成,刺激胃粘膜细胞增殖,抑制细胞凋亡,而这一过程可能与艾灸诱导HSP70表达有关。     

艾灸预处理对应激性胃黏膜损伤大鼠血清IL-1β、TNF-α和IL-10的影响

目的观察艾灸预处理对应激性胃黏膜损伤大鼠白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的影响,探讨艾灸预处理抗胃黏膜炎症损伤的作用机制。方法将SD大鼠随机分为空白组、模型组、艾灸穴位组、艾灸非穴点对照组。束缚冷应激法制作应激性胃黏膜损伤模型,按Guth法计算胃黏膜损伤指数（UI）,放射免疫法测定血清IL-1β、TNF-α和IL-10的含量。结果与模型组和艾灸非穴点对照组比较,艾灸足三里、中脘等穴可使应激性胃黏膜损伤UI明显下降、血清IL-1β含量降低、TNF-α含量降低、IL-10含量升高。结论艾灸足三里、中脘等穴预处理可促进大鼠胃黏膜损伤的修复、减轻急性炎症反应,可能是通过抑制细胞炎症反应的免疫促炎因子IL-1β、TNF-α和促进抗炎因子IL-10而达到其抗胃黏膜损伤作用。     

针灸对衰老模型小鼠皮肤中MDA、GSH-Px和Hyp影响的实验研究

目的:观察针灸对衰老模型小鼠皮肤组织形态的影响.方法:长期颈背部皮下注射D-半乳糖造成小鼠亚急性衰老模型,随机分为针刺组、艾灸组、模型组和空白组,检测皮肤组织中丙二醛(Malondialdehyde,MDA)含量、谷胱甘肽过氧化物酶(Glutathione Peroxidase,SH-Px)活力、羟脯氨酸(Hydroxyproline,Hyp)含量.结果:针灸足三里穴能够使衰老模型小鼠皮肤组织中的MDA含量减少,GSH-Px活力增强,Hyp含量增多.结论:针灸足三里穴对小鼠皮肤衰老具有拮抗作用.     

基于TLR4/MyD88/IκB信号通路探讨针刺合募配穴预防应激性胃溃疡的机制

目的:观察合募配穴对应激性胃溃疡(SGU)大鼠血清及胃组织氧化应激及炎性反应相关指标Toll样受体4(TLR4)、髓样分化因子88(MyD88)、核转录因子κB抑制蛋白α(IκB-α)的影响,探讨合募配穴预防SGU的机制。方法:Wistar大鼠随机分为空白组、模型组、阳性药组和合募配穴组,每组9只。采用束缚-水浸应激法建立SGU大鼠模型。造模前10 d,合募配穴组予以电针"中脘""足三里"(2 Hz, 0.6 mA),10 min/次,阳性药组予以奥美拉唑溶液(20 mg/kg)灌胃,两组均隔日干预1次,共10 d。肉眼及苏木精-伊红染色法观察各组大鼠胃黏膜的形态、计算溃疡指数(UI)和病变积分;TBA法和比色法检测各组大鼠血清和胃组织中丙二醛(MDA)、髓过氧化物酶(MPO)、谷胱甘肽过氧化物酶(GSH-Px)的水平;ELISA法检测各组大鼠血清肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)含量;Western blot法检测胃组织中TLR4、MyD88、IκB-α蛋白的相对表达量。结果:空白组大鼠胃黏膜层光滑完整,细胞排列整齐,未见毛细血管扩张充血和炎性细胞浸润。模型组大鼠胃黏膜上皮结构破坏,可见大量黏膜上皮细胞死亡及炎性细胞浸润。阳性药组和合募配穴组胃黏膜损伤和炎细胞浸润程度较模型组轻。与空白组比较,模型组大鼠的UI及胃黏膜病变积分显著升高(P<0.05),血清和胃组织中MDA含量、MPO活性显著升高(P<0.05),GSH-Px活性显著降低(P<0.05),血清中TNF-α、IL-6含量显著增加(P<0.05),胃组织中TLR4、MyD88蛋白表达水平显著升高(P<0.05)、IκB-α表达水平显著降低(P<0.05);与模型组比较,阳性药组与合募配穴组的UI及胃黏膜病变积分显著降低(P<0.05),血清和胃组织中MPO活性显著降低(P<0.05)、GSH-Px活性显著升高(P<0.05),血清中TNF-α、IL-6含量显著降低(P<0.05),胃组织中MDA含量和TLR4、MyD88蛋白表达水平显著减少(P<0.05)、IκB-α表达水平显著增加(P<0.05);合募配穴组血清TNF-α的含量低于阳性药组(P<0.05)。结论:合募配穴预电针可保护SGU大鼠的胃黏膜组织,其机制可能与抗氧化、抗炎和调节TLR4/MyD88/IκB信号通路有关。     

艾灸对胃黏膜损伤大鼠胃黏膜表皮生长因子、转化生长因子-α及其受体的影响

目的:观察艾灸对应激性胃黏膜损伤大鼠胃黏膜表皮生长因子(EGF)、转化生长因子-α(TGF-α)与表皮生长因子受体(EGFR)的影响,探讨艾灸修复胃黏膜损伤的作用机制。方法:SD大鼠随机分为正常组、模型组、艾灸组,每组12只。束缚冷应激法制作应激性胃黏膜损伤大鼠模型,在造模之前,艾灸组选取"足三里""中脘""脾俞""胃俞"行艾灸预处理8d。酶联免疫法检测胃黏膜组织中EGF、TGF-α的含量,免疫组化法检测胃黏膜EGFR蛋白表达。结果:与正常组比较,模型组大鼠胃黏膜TGF-α的含量明显增加(P<0.05);与模型组比较,艾灸组大鼠胃黏膜EGF、TGF-α的含量及EGFR蛋白表达明显上调(P<0.05,P<0.01)。结论:预先艾灸"足三里""中脘""脾俞""胃俞"可上调应激性胃黏膜损伤大鼠胃黏膜EGF、TGF-α的含量及EGFR表达,可能与其对胃黏膜的保护作用有关。     

芩连合剂对实验性胃溃疡大鼠胃黏膜保护作用的机制研究

目的探讨芩连合剂(QLHJ)对急性胃溃疡大鼠胃黏膜的保护作用及其作用机制.方法采用无水乙醇灌胃法复制大鼠急性胃溃疡模型,测定胃黏膜细胞内丙二醛(MDA)、超氧化物歧化酶(SOD)的表达、谷胱苷肽过氧化物酶(GSH-Px)活力及钙离子浓度.结果模型组大鼠胃黏膜细胞内钙离子浓度较其他各组明显升高,与芩连合剂组和正常组比较有非常显著性差异(P＜0.01);SOD、GSH-Px的表达较其他各组明显降低,MDA表达较其他各组明显升高,与芩连合剂组、地尔硫组和正常组比较有显著性差异(P＜0.05,P＜0.01).结论QLHJ通过降低胃黏膜细胞内钙离子浓度的水平,降低胃黏膜细胞内MDA的表达和提高胃黏膜细胞内SOD和GSH-Px的表达,从而发挥其保护胃溃疡大鼠胃黏膜细胞的作用.     

针灸足三里穴对衰老模型小鼠皮肤中MDA、GSH-Px和Hyp的影响

目的观察了针灸足三里穴对衰老小鼠皮肤组织形态的影响。方法长期颈背部皮下注射D-半乳糖造成小鼠亚急性衰老模型,随机分为针刺组、艾灸组、模型组和空白组,检测皮肤组织中丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活力、羟脯氨酸(Hyp)含量。结果针灸足三里穴能够使衰老模型小鼠皮肤组织中的MDA含量减少,GSH-Px活力增强,Hyp含量增多。结论针灸足三里穴对小鼠皮肤衰老具有拮抗作用。     

足三里配不同穴改善急性胃黏膜损伤作用的对比研究

目的:观察足三里配不同穴改善急性胃黏膜损伤的作用,探讨其机理。方法:100只大鼠随机分为10组,即“足三里”组(A组)、“足三里”配“内关”组(B组)、“足三里”配“中脘”组(C组)、“足三里”配“公孙”组(D组)、“足三里”配“内关”“中脘”组(E组)、“足三里”配“内关”“公孙”组(F组)、“足三里”配“中脘”“公孙”组(G组)、“足三里”配“内关”“中脘”“公孙”组(H组)、模型组(I组)、空白对照组(J组),每组10只。用无水乙醇按0·6mL/100g灌胃,造成胃黏膜损伤模型。检测各组治疗结束后胃黏膜损伤指数(UI)、表皮生长因子(EGF)、一氧化氮(NO)、胃泌素(GAS)含量。结果:各电针组EGF含量明显高于模型组(P<0·01),并能提高NO含量,降低GAS含量,与模型组比较差异有显著性意义(P<0·05);A组、B组、D组之间比较差异无显蓍性意义,而C组、F组、H组与A组比较差异有显著性意义;H组与其他各电针组比较差异有显著性意义。结论:“足三里”不同配穴能改善急性胃黏膜损伤程度,尤以“足三里”配“内关”“中脘”“公孙”组改善急性胃黏膜损伤作用最强。