肾衰91冲剂对不同慢性肾衰大鼠毒素作用的实验研究

目的探讨肾衰９１冲剂对不同慢性肾衰大鼠毒素作用的机理。方法采用肾衰９１冲剂治疗０．７５％腺嘌呤和ｐｌａｔ法诱发慢性肾衰。结果与模型组相比,肾衰９１冲剂组血尿素氮、肌酐、中分子物质、甲基胍、胍基琥珀酸、磷显著降低,钙升高,两组上述各项指标均有显著性差异（Ｐ＜０．０５～０．０１）。结论肾衰９１冲剂组有降低尿毒证毒素,调整钙磷代谢紊乱的作用。     

丹参可改善肾功能

日本西冈五夫研究室等研究单位,将丹参浸膏投与腺嘌呤诱发肾功能不全的大白鼠时,可明显降低其血中尿毒症性毒素(尿素、肌酸酐、甲基胍、胍基琥珀酸),具有增加尿量,促进尿中尿素、肌酸酐、钠、钾、磷酸的排泄,增加肾组织的血流量,增强肾小球滤过率等改善肾功能不全     

虫草多糖对腺嘌呤诱发慢性肾衰大鼠的治疗作用

目的探讨虫草多糖对腺嘌呤诱发的慢性肾功能衰竭大鼠的治疗作用。方法采用腺嘌呤灌胃复制大鼠慢性肾衰(CRF)模型,将大鼠随机分为正常组,模型组,虫草多糖高、中、低剂量组和地塞米松组,观察治疗后大鼠的肾功能、血液生化指标、肾病理、肾脏指数及血清TNF-α的变化。结果虫草多糖各组肾功能受损程度明显轻于模型组,肾脏病理损伤明显减轻,血清TNF-α的含量明显减少,肾脏脂数明显下降。结论虫草多糖能够防治腺嘌呤诱发的慢性肾功能衰竭,有效预防慢性肾衰的发生、发展,改善肾功能。     

葆肾3号影响慢性肾功能衰竭的实验研究

目的:研究葆肾3号对慢性肾衰大鼠尿毒症毒素及其细胞凋亡的影响。方法:制作大鼠5/6肾切除肾衰模型,分别给予葆肾3号方及蒙诺治疗76d后,检测大鼠血清肌酐(Scr)、尿素氮(BUN)、甲基胍(MG)、胍基琥珀酸(GSA)、中分子物质(MMS)、24h尿蛋白定量以及肾组织细胞凋亡基因Fas/FasL的变化。结果:经葆肾3号方治疗后的慢性肾衰大鼠,血清BUN、Scr、MG、GSA、MMS水平明显下降,尿蛋白定量降低,与对照组相比有显著性差异;Fas/FasL表达在肾切除各组均显著增多,治疗后明显减少,尤以治疗组显著。结论:葆肾3号可以显著清除尿毒症毒素水平、改善肾功能,通过降低Fas/FasL基因表达起调控肾实质细胞调亡作用。     

肾衰冲剂对尿毒症毒素作用的实验研究

临床研究表明肾衰冲剂能明显改善慢性肾功能衰竭患者临床症状,显著降低患者血中大、中、小分子毒性物质,本研究采用Platt 法诱发大鼠慢性肾衰模型,观察肾衰冲剂对肾衰大鼠肾功能的影响,并探讨其作用机理。现报告如下。材料与方法1 材料1.1 动物 SD 大鼠48只,雄性,体重195～200g。上海中医药大学动物实验中心提供。喂饲市售固体饲料,饮用自来水,自由饮水和摄食。1.2 药物肾衰冲剂由党参15g 丹参15g 炮附子10g 仙灵脾15g 黄连5g 制大黄15g 虫草菌丝5g 组     

右归丸对慢性肾衰大鼠肾保护作用的实验研究

目的：观察右归丸对慢性肾衰大鼠的治疗作用。方法：采用腺嘌呤灌胃建立大鼠慢性肾衰模型，将大鼠随机分为正常组、模型组、右归丸组，观察治疗后大鼠的肾功能、肾病理、血中ET-1及AngⅡ含量的变化。结果：右归丸组大鼠血中尿素氮（BUN）、肌酐（Scr）含量低于模型组，肾脏病理明显改善，血清ET-1、AngⅡ含量明显减少。结论：温补肾阳方剂右归丸能延缓慢性肾衰的进展，可作为治疗慢性肾衰的基础方剂。     

肾衰灵对慢性肾衰大鼠血脂和肾功能的影响

目的：观察肾衰灵对慢性肾衰（CRF）大鼠肾功能、血脂的影响。方法：采用5/6肾切除法建立慢性肾衰肾小球硬化大鼠模型,随机分为正常组、模型组、赖诺普利组、肾衰灵组,8周后测定各组大鼠肾功能、血脂。结果：模型组大鼠24小时尿蛋白、血肌酐、血尿素氮及血脂水平与正常组相比均明显增加（P〈0.05）,肾衰灵、赖诺普利组各指标明显低于模型组（P〈0.05）,肾衰灵组明显低于赖诺普利组（P〈0.05）。结论：肾衰灵有降低尿蛋白,改善肾功能,调节脂质代谢紊乱的作用,能延缓肾小球硬化进展,进而达到治疗肾功能衰竭的目的。     

精芪口服液对肾衰大鼠肾功能的影响

 为寻找治疗肾衰的有效药物，研究了精芪口服液对用甘油诱发的急性肾功能衰竭（ＡＲＦ）大鼠肾功能的影响。试验结果表明，精芪口服液可使肾衰大鼠２４ｈ尿量增加、血ＢＵＮ、血肌酐、血清Ｋ￣＋及Ｎａ￣＋降低，此作用优于地塞米松，为临床应用精芪口服液治疗ＡＲＦ提供实验资料。     

肾衰饮对慢性肾功能衰竭大鼠肾脏bmp-7、pdgf、timp-1 mRNA表达的影响

目的:通过观察肾衰饮对慢性肾功能衰竭大鼠肾脏bmp-7、pdgf、timp-1 mRNA表达的影响,探讨肾衰饮延缓慢性肾功能衰竭的药效学机制。方法:52只SD大鼠随机分为正常组、模型组、尿毒清组及肾衰饮组。采用腺嘌呤灌胃法制作大鼠肾衰模型。造模成功后肾衰饮组大鼠给予肾衰饮煎剂灌胃,尿毒清组大鼠给予尿毒清颗粒灌胃,各组大鼠连续给药4周,全自动生化分析仪检测血肌酐(SCr)、尿素氮(BUN)及白蛋白水平,HE染色及Masson染色观察各组大鼠肾组织病理变化,Real time-PCR技术检测大鼠肾脏bmp-7、pdgf、timp-1 mRNA表达情况。结果:与正常组比较,模型组血肌酐、尿素氮水平显著升高(P<0.01),白蛋白水平显著降低(P<0.01);与模型组比较,肾衰饮组和尿毒清组大鼠血肌酐、尿素氮水平均显著降低(P<0.01);白蛋白水平均显著升高(P<0.01)。HE及Masson染色显示模型组大鼠肾脏组织发生明显的病理学改变,肾衰饮组及尿毒清组大鼠肾脏病理改变发生明显改善。与正常组相比,模型组大鼠肾脏bmp-7基因mRNA表达水平显著下降(P<0.01),pdgf、timp-1基因mRNA表达水平显著升高(P<0.01);与模型组比较,肾衰饮组大鼠肾脏bmp-7基因mRNA表达水平显著升高(P<0.01),pdgf、timp-1基因mRNA表达水平显著下降(P<0.01)。结论:肾衰饮可能通过调节慢性肾功能衰竭大鼠肾脏bmp-7、pdgf、timp-1 mRNA表达,发挥其延缓慢性肾功能衰竭进展的重要生物学作用。     

肾衰宁片治疗慢性肾功能不全临床观察

目的：观察肾宁片治疗慢性肾功能不全的临床疗效。方法：采用随机对照治疗组（30例）给予口服肾衰宁片5片，每日3次，对照组给予口服包酣氧淀粉1包（5g)每日2次，两组均以1个月为1疗程。观察两组治疗前后的肾功能及中医临床疗效。结果：治疗组与对照组比较，血尿素氮（BUN）下降明显，P＜0．05，血肌酐（Cr)亦下降，中医临床症状改善显著，有效率达83．33％。明显高于对照组60％。结论：肾衰宁片能改善肾功能，延缓慢性肾功能衰竭进展。     

微波消解-电感耦合等离子体质谱法测定脉络宁注射液中25种矿物质元素

目的建立微波消解-电感耦合等离子体质谱(ICP-MS)直接稀释测定脉络宁注射液中25种矿物质元素(Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V、As、Cd和Hg)的方法。方法分别对微波消解条件和测试条件进行考察;样品经微波消解后,采用电感耦合质谱仪测定25种矿物质元素,并对测定方法学进行考察。结果确定最佳消解条件为3步缓慢升温:400 W 80℃升温10 min,保留5 min;600 W 120℃升温10 min,保留5 min;900 W 200℃升温20 min,保留20 min;25种矿物质元素在各自的线性范围内线性关系良好,r≥0.999 6,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为94.7%~106.1%,RSD在0.34%~2.79%。脉络宁注射液中检测出Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V,未检出As、Cd和Hg。结论该方法简便、迅速、准确,适用于脉络宁注射液中25种矿物质元素的同时测定。     

Die Lateralität und Lateralitätsinstabilität in der Diagnostik und Therapie

The disturbance and instability of laterality are obstacles to diagnostics and therapy. Correction prior to starting therapy is required. They also predispose toward defined health problems and unspecific diseases. Numerous research activities provide evidence of the relevance of undisturbed laterality in diagnostics and therapy. Techniques of testing and therapeutic corrections will allow for optimized therapy success.     

Yerba Mate or Paraguay Tea

正Beverages based on Ilex paraguariensis A.St.-Hil.are used in the south Brazil and other Latin American countries located at the so-called southern cone.It is known as chimarro or mate in south and southeast Brazil,tererêin     

Journal of Acupuncture and Tuina Science Instructions for Authors

The Journal of Acupuncture and Tuina Science, a journal with an international scope （IS SN 1672-3597, CN 31-1908/R, Bimonthly）, is embodied by ＇Springer Verlag＇ Database, Index Copernicus （IC） and Chinese Scientific and Technical Paper and Citations Data （CSTPCD）. You can search full text on http：//www, springerlink, com/content/1672 -3597.     

Ethnopharmacologic study of chinese rhubarb

This paper deals with an interdisciplinary study covering historic, botanical, phytochemical, pharmacological and clinical aspects of rhubarb and related species, to lay stress on the correlation between plant phylogeny, chemical constituents and purgative activity.

It was found that the official rhubarbs were exclusively restricted in the Sect. Palmata e.g. Rheum palmatum R. palmatum var. tanguticum R. officinale; the following criteria may serve as their standard, viz., the presence of sennoside derivatives and rhein, the occurrence of the reduced form of rhein and aloe-emodin, the leaves with any kind of palmate incision. Comprehensive multivariate analyses showed that there is a very close relationship between the leaf incision, existence of sennosides or rhein and purgative activity.     

基于ICP-MS法的加味左金丸中无机元素分析

目的 通过电感耦合等离子体质谱（inductively coupled plasma mass spectrometry,ICP-MS）法建立加味左金丸中Cd、Pb、As、Hg、Co、V、Ni、Cu、Li、Sb、Ba、Mo、Sn、Cr、Na、Mg、Al、Ca、Ti、Mn、Fe、Zn、Ga、Se、Sr、Tl共计26种无机元素的测定方法。方法 加味左金丸通过微波消解法处理后,根据相对分子质量的大小选择内标物,其中⁷Li、²³Na、²⁴Mg、²⁷Al、⁴⁰Ca、⁴⁸Ti、⁵¹V、⁵²Cr、⁵⁵Mn、⁵⁶Fe、⁵⁸Ni、⁵⁹Co、⁶³Cu、⁶⁶Zn、⁷⁰Ga、⁷⁵As、⁷⁷Se、⁸⁶Sr以⁷²Ge作为内标;⁹⁵Mo、¹¹⁴Cd、¹¹⁸Sn、¹²¹Sb、¹³⁷Ba以¹¹⁵In作为内标;²⁰²Hg、²⁰⁵Tl、²⁰⁸Pb以²⁰⁹Bi作为内标。对标准品溶液、空白溶液与供试品溶液进行分析,采用标准曲线法进行定量分析。通过ICP-MS法进行测定。结果 26种无机元素线性的相关系数r ≥ 0.999 6,检出限为0.001~1.500μg/L,定量限为0.01~5.00 μg/L,精密度与重复性试验的RSD均小于5%,平均回收率在82.64%~106.44%,RSD均小于5%。对3个厂家的12批样品进行了测定,26种元素的含量差异较大,其中Na、Mg、Ca、Fe 4种元素的含量比较高,均大于500 μg/g,Cd、Pb、As、Hg、Co、Li、Sb、Mo、Sn、Cr、Se、Tl的含量比较低,均小于1 μg/g。由结果可知,人体的常量元素,如Na、Mg、Ca的含量比较高,Cd、Pb、As、Hg等有害元素含量比较低。根据《中国药典》2020年版一部的要求,本品中Cd、Pb、As、Hg与Cu均符合规定。结论 该方法快速、准确,可以用于加味左金丸中无机元素的测定。     

Wirksamkeit der Akupunktur bei der Therapie von idiopathischen Schmeckstörungen: Eine randomisierte Placebo-kontrollierte Studie

Background

At present, the state-of-the-art medication in treating idiopathic taste disorder (gustatory dysfunction) is zinc. If zinc medication was unsuccessful, not tolerated or if it is contraindicated the persons affected can hardly be helped.

Objectives

In a randomized placebo-controlled clinical trial an attempt was made to determine the efficacy of acupuncture.

Methods

The treatment group was treated with acupuncture needles, the placebo group with an inactive acupuncture-laser. Endpoints of the study were changes in the taste test, the quality of life and the severity of symptoms of depression (BDI) as well as mood changes (ZMS). Satisfaction with the success of the respective therapy was also evaluated. Data were collected before and after therapy and again for the treatment group 10 weeks after completion of the treatment period.

Findings

Treatment with real acupuncture showed a significant improvement in gustatory function as well as a significant improvement in psychological symptoms. Greater satisfaction with the treatment results was evident in the treatment group. The treatment results of acupuncture remained stable over a period of ten weeks after completion of the treatment.

Conclusion

Acupuncture was effective in the therapy of idiopathic taste disorders and therefore it is an effective alternative when zinc medication is not tolerated or unsuccessful. But it is also suitable as a therapy of first choice. Acupuncture can be considered as an extension of the therapeutic spectrum in the difficult treatment of taste disorders.     

Erstaunliches Ergebnis einer Schädelakupunktur nach Yamamoto bei Hemihypsarrhythmie rechts

A girl, three and a half years old at the respective date, premature in 25th week of gestation, had been presented. On day three after birth, she had had a IVH III° right and left with severe involvement of parenchyma and other multiple complications, e. a. infant respiratory distress syndrome up to the 42nd day postpartum, and epileptic attacks with apnoeic events.     

Veränderungen der Herzratenvariabilität (HRV) während Low Level Lasernadelakupunktur: Placebokontrollierte Studie an 19 herzgesunden Probanden

Background

Experimental and clinical studies postulate a neurobiological mechanism of acupuncture by modulating central pain systems, neurohormones and -transmitters. It seems to be quite likely that the autonomic nervous system (ANS) plays an important mediating role in this neuromodulation.

Aim

In measuring heart rate variability (HRV), as a non-invasive approach to the ANS-function, the object of this study was to monitor probable acupuncture-induced changes of autonomic balance. The innovative technology of laser-needle acupuncture offers for the first time a stimulation method which fulfils the demands on a placebo-controlled acupuncture trial not requiring controversial sham acupuncture.

Method

19 healthy voluntary subjects underwent 3 treatments in randomized sequence, consisting of placebo-, 1-point- (PC 6) and simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle-stimulation over 20 minutes. Before, during and after placebo-/verum laser-needle acupuncture, HRV-data for normalized HF(high frequency)- and LF(low frequency)-Power (nHF, nLF) and their quotient nLF/nHF obtained by power spectrum analysis (FFT), were measured and analyzed statistically at 5 different time points (ANOVA repeated measures, p < 0,05).

Results

During both verum applications, the 1-point- (PC 6) as well as the simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle stimulation, significant changes of the ANS-activity were found in comparison to the placebo application. The most significant modification of HRV was an increase of nHF (p < 0,05 (PC 6), p < 0,0001 (PC 6, LR 3, SI 3)) as an expression of growing vagal activity during acupuncture.

Conclusion

Laser-needle acupuncture causes in 1-point-stimulation (PC 6) as well as in simultaneous 3-point-stimulation (PC 6, LR 3, SI 3) significant changes in HRV in comparison to placebo application. HRV-monitoring seems to be a suitable approach in exploration of acupuncture-induced changes of ANS and could possibly be established in combination with laser needle acupuncture as a standard for further scientific and clinical acupuncture investigations which are greatly needed.