KAll基因在肝硬化和肝细胞癌组织中的表达及其意义

目的研究转移抑制基因KAIl在肝硬化和肝细胞癌组织中的表达及该基因与肝细胞癌患者临床资料间的关系.方法采用Northern blot方法研究20例肝细胞癌组织、14例肝硬化组织和10例正常肝组织中KAll基因mRNA的表达水平,经mRNA的定量分析及统计处理判定KAIl基因与肝细胞癌患者临床特征的关系.结果肝硬化和肝细胞癌组织中KAll mRNA表达水平(0.113±0.110;0.139±0 078)显著低于正常肝组织中的表达(0.316±0.121),(P=0.0003;P=0.0000);肝硬化和肝细胞癌组织中KAll基因mRNA的表达无显著差异(p=0.4152);KAIlmRNA在不同分化程度的肝细胞癌组织中的表达无显著差异(P=0.8261),而Ⅲ期肝细胞癌组织中的KAll基因表达显著低于Ⅱ期的肝细胞癌组织(P=0.0221)结论转移抑制基因KAI1在肝硬化阶段亦有低表达;肝癌组织中KAI1基因的低表达与肝癌的转移有关.     

上皮-间充质转换与血管生成拟态形成在肝细胞癌中的表达及临床意义

目的研究上皮-间充质转换(epithelial-mesenchymal transition,EMT)相关蛋白及血管生成拟态(vasculogenic mimicry,VM)在肝细胞癌(hepatocellular carcinoma,HCC)中的表达及其与复发、转移的关系,探讨EMT在HCC VM形成中的可能作用机制。方法应用免疫组化法检测200例临床资料和随访资料完整的手术切除HCC病例中E-cadllerin的表达,CD31/PAS双重染色观察HCC中VM的分布特征。比较其在HCC中的临床病理意义和预后的关系。结果 200例HCC组织中38例存在VM,阳性率为19.0%;VM的阳性表达与病理分级、TNM分期及预后有关(P0.05)。200例HCC组织E-cadllerin表达97例,阳性率为48.5%;E-cadllerin的表达与病理分级、TNM分期、预后有关(P0.05)。且VM阴性组E-cadllerin表达率均高于VM阳性组(P0.05)。结论 HCC组织中存在VM;VM、E-cadllerin与HCC的侵袭、转移及预后密切相关。HCC可能通过EMT过程促进VM形成。     

p73基因在肝细胞癌中的表达及其临床意义

背景:p73基因是新发现的一种抑癌基因,其在肿瘤发生、发展中的作用尚不明确.目的:探讨p73基因在肝细胞癌中的表达、突变程度及其与临床预后的关系.方法:采用定量逆转录聚合酶链反应(RT-PCR)检测35例肝细胞癌和配对癌旁组织,以及10例转移淋巴结和10例正常淋巴结组织中p73基因的表达;采用内含子PCR产物StyⅠ酶切分析法调查标本的杂合性;采用PCR-单链构象多态性(SSCP)检测p73基因的突变情况.结果:35例肝细胞癌组织中有26例p73 mRNA呈高表达,癌旁组织则呈低表达,两者表达率有显著差异(P＜0.01);20例中高分化癌组织中有13例p73 mRNA呈中高表达,15例低分化癌组织中有13例p73 mRNA呈中高表达,两者阳性率之间有显著差异(P＜0.01);18例Ⅰ～Ⅱ期肝细胞癌标本中有9例p73 mRNA呈中高表达,17例Ⅲ～Ⅳ期肝细胞癌标本中p73 mRNA均呈中高表达,两者阳性率之间有显著差异(P＜0.05).10例转移淋巴结组织中p73 mRNA均呈高表达,而10例正常淋巴结组织中均呈低表达,两者之间有显著差异(P＜0.01).12例杂合性肝细胞癌组织标本中,有10例p73基因存在G/C:A/T双等位基因表达,在癌旁组织中则均为G/C表达,未见A/T表达.肝细胞癌和癌旁组织中未见p73基因突变.结论:p73基因的表达与肝细胞癌的分化程度、临床分期和预后有关,突变可能不是p73基因起作用的主要方式.     

RNA干扰HIF-1α对食管鳞癌血管生成拟态的影响

背景:研究表明大量高度恶性肿瘤组织中存在血管生成拟态(VM),其分子机制已形成相关假说,但确切机制和关键信号通路尚未明确。目的:探讨缺氧诱导因子(HIF)-1α在食管鳞癌VM形成中的作用。方法:设计合成3种HIF-1α-siRNA质粒,测序后瞬时转染293T细胞,蛋白质印迹法鉴定质粒的干扰效果。将筛选出的pGCsi-HIF3质粒稳定转染食管鳞癌细胞株Eca109和TE13,以蛋白质印迹法检测干扰效果,三维培养法观察VM形成情况,蛋白质印迹法检测VE-cadherin、EphA2、LN5γ2和MMP2蛋白表达。结果:成功构建了3个靶位的质粒,其中pGCsiHIF3的干扰效果最好。pGCsi-HIF3稳定转染食管鳞癌细胞株Eca109和TE13后,与对照组相比,HIF-1α表达明显降低,细胞体外管道形成能力被明显抑制(P0.05)。常氧下转染组细胞中VE-cadherin、EphA2、LN5γ2均显著下调(P0.05),MMP2表达无明显差异,且缺氧条件下上述指标蛋白表达无明显增加。结论:Eca109和TE13细胞能形成管状结构,HIF-1α可能通过调节VE-cadherin、EphA2、LN5γ2等的表达而调节食管鳞癌VM的形成。     

小干扰RNA沉默巨噬细胞移动抑制因子基因对肝癌细胞p27表达的影响

目的 观察巨噬细胞移动抑制因子(MIF)和细胞周期调控因子p27在肝细胞癌中表达的相互关系,探讨小干扰RNA(siRNA)沉默MIF基因对肝癌细胞p27表达的影响.方法 免疫组织化学法和荧光定量PCR法检测MIF、p27的蛋白和mRNA在肝癌及其癌旁组织中的表达情况.化学合成MIF siRNA和对照siRNA,脂质体法转染肝癌细胞PLC和Hep3B,荧光定量PCR法检测MIF和p27 mRNA在实验组及对照组中的表达情况.根据不同资料分别采用X2检验、Logistic回归分析或单因素方差分析.结果 MIF蛋白及其mRNA在肝癌组织中过表达,在癌旁组织中低表达;p27蛋白及其mRNA在癌组织中低表达,在癌旁组织中高表达.Logistic回归分析提示MIF为肝癌发生的危险因素,p27为保护因素.MIF mRNA在肝癌细胞株中过表达(F=61.036,P＜0.01),p27 mRNA在正常肝细胞L02中高表达(F=529.853,P＜0.01).经MIFsiRNA转染后,MIF mRNA在PLC及Hep3B中的表达水平降低,并且呈剂量依赖关系(F值分别为320.1和201.2,P值均＜0.01);p27 mRNA伴随MIF mRNA的降低而增加(F值分别为419.4和459.9,P值均＜0.01).结论 MIF在肝细胞癌中过表达,MIF siRNA能特异性抑制其在肝癌细胞中的表达;MIF可能参与了p27基因表达的调控.     

CD133和VEGF在肝细胞癌表达及其预后预测价值

目的研究肝细胞癌干细胞标记分子CD133与血管内皮生长因子VEGF在肝细胞癌组织中的表达及其预后价值。方法应用免疫组织化学染色方法检测CD133、VEGF在190例肝细胞癌组织中的表达水平,分析其与各项临床病理指标和无瘤生存之间的关系。结果 CD133、VEGF在肝细胞癌组织中的阳性表达率分别为22.1%(42/190)和52.1%(99/190)。CD133的表达水平与乙型肝炎病毒感染、肝细胞癌分化程度、镜下血管侵犯比较,差异有显著统计学意义(P0.05);CD133表达与VEGF表达水平比较,差异有显著统计学意义(P0.001)。生存分析表明,CD133阳性组术后无瘤生存期明显短于CD133阴性组,差异有显著统计学意义(P=0.036)。结论肝细胞癌组织中,CD133高表达与VEGF高表达、肝细胞癌低分化以及镜下血管侵犯密切相关;CD133表达水平与肝细胞癌术后无瘤生存呈负相关,CD133可能通过调节VEGF表达水平影响肿瘤局部新生血管形成从而影响肝细胞癌预后。     

肝细胞癌患者FOXM1的表达及临床意义

目的探讨FOXM1基因在肝细胞癌及对应癌旁组织中的表达水平及临床意义。方法采用qRT-PCR技术检测FOXM1 mRNA在肝细胞癌及配对癌旁组织中的表达。结果肝细胞癌组织中FOXM1 mRNA表达高于癌旁组织(P0.01);FOXM1 mRNA在低分化肝细胞癌组织中的表达量显著高于中-高分化组(P0.01);FOXM1 mRNA高表达与肝细胞癌患者肿瘤数目(P=0.010)、肿瘤分化程度(P=0.002)、脉管浸润(P=0.029)、血清AFP水平(P=0.004)、临床分期(P=0.003)显著相关;FOXM1mRNA过表达的肝细胞癌患者生存率下降(P=0.010)。结论 FOXM1在肝细胞癌组织中呈高表达,FOXM1基因有可能成为肝细胞癌早期诊断和预后判断的分子标记物。     

环氧化酶抑制剂吲哚美辛对胰腺癌新生血管生成的抑制作用

目的 通过观察环氧化酶(COX)抑制剂吲哚美辛对胰腺癌血管内皮生长因子(VEGF)表达及对体内外血管生成的的影响,探讨其对胰腺癌生长抑制的作用机制.方法 用RT-PCR方法检测不同浓度吲哚美辛对BxPC3细胞VEGF mRNA表达的调节作用;应用小管形成实验研究吲哚美辛对ECV304细胞体外血管生成的影响;建立裸鼠胰腺癌BxPC3细胞种植瘤模型,应用吲哚美辛给荷瘤裸鼠灌胃(3 mg·kg-1·d-1体重,共4周),观察其对种植瘤生长曲线、瘤重及种植瘤组织VEGF mRNA表达的影响;采用Ⅷ因子免疫组化染色评估瘤组织中微血管密度(MVD).结果 (1)小管形成实验中,吲哚美辛组ECV304细胞数较少,细胞排列差,偶可见条索状细胞链,中空闭合管状结构缺如.(2)吲哚美辛呈剂量依赖性抑制BxPC3细胞VEGF mRNA表达.(3)应用吲哚美辛给荷瘤裸鼠灌胃4周后,平均瘤重(0.495±0.31)g,显著小于对照组瘤重(1.57±1.06)g,抑瘤率为67%.吲哚美辛显著下调种植瘤VEGF mRNA表达.(4)对照组MVD为5.98±1.27,吲哚美辛组为2.02±0.28,差异有显著性(P＜0.01).结论 COX抑制剂吲哚美辛可下调VEGF mRNA的表达,抑制胰腺癌体内、体外血管的生成,抑制裸鼠种植瘤新生血管的生成,这可能是其抑制胰腺癌裸鼠种植瘤生长的重要原因.     

肝细胞癌组织中miR-21、miR-26a及miR-338的表达及意义

目的探讨肝细胞癌组织中miR-21、miR-26a及miR-338在肝细胞癌发生发展中的作用。方法选择本院肿瘤中心组织标本库的肝细胞癌组织标本30例份(肝细胞癌组)及相应的癌旁组织标本30例份(对照组),采用Real-time PCR法检测两组miR-21、miR-26a及miR-338的表达,分析其表达与肝细胞癌患者临床病理特征的关系。结果肝细胞癌组miR-21的表达高于对照组,miR-26a、miR-338表达均低于对照组(P均<0.01)。肝细胞癌组淋巴结转移、伴有肝硬化、高分化及TNM分期Ⅲ~Ⅳ期者miR-21相对表达量较高,淋巴结转移、不伴肝硬化、中低分化者miR-26a相对表达量较高,无淋巴结转移、TNM分期Ⅲ~Ⅳ期者、有门静脉癌栓者miR-338相对表达量较高(P均<0.05)。miR-21的表达与肝硬化、瘤栓、肿瘤分化和TNM分期等呈正相关(P均<0.05);miR-26a的表达与肝硬化和肿瘤分化呈负相关(P<0.05);miR-338表达与肿瘤TNM分期和门静脉癌栓呈正相关(P均<0.05)。结论miR-21表达增高、miR-26a及miR-338表达降低可促进肝癌的发生及发展。     

生长抑素受体表达与肝细胞癌病理特征及预后的关系

目的研究生长抑素受体(SSTR)在肝细胞癌组织中表达水平及其与肝细胞癌临床病理特征及预后的关系。方法所有肝细胞癌标本取自2012年7月-2014年12月就诊于解放军第三医院及大连医科大学附属二院肝胆科行切除术后经病理组织学检查证实为HCC的患者(n=80,试验组),另选取同期疑似肝部疾病且行肝穿刺确诊的非肝细胞癌患者(n=80,对照组)。采用RT-PCR检测SSTR-2、SSTR-3 mRNA水平,采用免疫组化检测SSTR-2、SSTR-3蛋白表达的水平。计量资料组间比较采用t检验,计数资料组间比较采用χ2检验,Kaplan-Meier法分析患者生存情况,Cox分析肝细胞癌患者预后的影响因素。结果对照组SSTR-2、SSTR-3 mRNA及蛋白均显著高于试验组(t值分别为6.456、8.128,χ2值分别为7.992、9.157,P值均<0.05)。单因素分析显示,SSTR-2和SSTR-3 mRNA与肿瘤结节(t=6.533、5.041,P值均<0.05)、分化程度(t=4.672、4.013,P值均<0.05)、浸润深度(t=6.735、7.019,P值均<0.05)、病毒性肝炎(t=4.929、4.535,P值均<0.05)、酒精性肝炎(t=4.032、4.362,P值均<0.05)、糖尿病(t=4.372、6.293,P值均<0.05)等显著相关;SSTR-2和SSTR-3蛋白与肿瘤结节(χ2=25.223、15.399,P值均<0.05)、分化程度(χ2=7.535、10.944,P值均<0.05)、浸润深度(χ2=22.520、9.968,P值均<0.05)密切相关。SSTR-2和SSTR-3阴性表达组的累积术后无瘤生存率明显低于SSTR-2和SSTR-3亚型的阳性表达组(P值分别为0.015、0.004),SSTR-2和SSTR-3阴性表达组的术后总体生存率明显低于SSTR-2和SSTR-3亚型的阳性表达组(P值分别为0.009、<0.001)。Cox模型分析显示,SSTR-2蛋白、SSTR-3蛋白、肿瘤结节数目、肝硬化、静脉浸润是HCC术后总生存期的独立危险因素(P值均<0.05)。结论SSTR-2和SSTR-3亚型在肝细胞癌组织中表达低于非肝细胞癌患者,其低表达与肝细胞癌的常见侵袭转移特征及不良预后密切相关。生长抑素受体可能成为肝细胞癌的预后标志物。     

A Call to Action to Enhance Filovirus Disease Outbreak Preparedness and Response

The frequency and magnitude of recognized and declared filovirus-disease outbreaks have increased in recent years, while pathogenic filoviruses are potentially ubiquitous throughout sub-Saharan Africa. Meanwhile, the efficiency and effectiveness of filovirus-disease outbreak preparedness and response efforts are currently limited by inherent challenges and persistent shortcomings. This paper delineates some of these challenges and shortcomings and provides a proposal for enhancing future filovirus-disease outbreak preparedness and response. The proposal serves as a call for prompt action by the organizations that comprise filovirus-disease outbreak response teams, namely, Ministries of Health of outbreak-prone countries, the World Health Organization, Médecins Sans Frontières, the Centers for Disease Control and Prevention—Atlanta, and others.     

Control of intraabdominal hemorrhage and shock: a comparison of fluid resuscitation, MAST, and balloon occlusion

Our study examined the efficacy of four treatment modalities in controlling hemorrhage and achieving hemodynamic stabilization in hemorrhagic shock: intravenous fluid replacement (IV); military antishock trousers used concomitantly with fluids (MAST); balloon occlusion at the level of the diaphragm with concomitant fluid replacement (balloon); and a combination of MAST inflation, balloon occlusion, and fluid resuscitation (MAST and balloon). Twenty-eight mongrel dogs were anesthetized, and the spleen was exposed and completely crushed. The abdomen was closed, and treatment was initiated and continued for four hours or until the dog died. For all conditions the hematocrit dropped during the course of the experiment; balloon occlusion was effective at slowing this drop (P less than .0001), but MAST had no statistically significant effect. Animals with balloons bled more slowly into the abdominal cavity than did animals in the other two groups (P less than .0001). MAST also were effective at slowing the bleeding (P less than .05). Of the balloon and the MAST and balloon dogs, all except one survived the entire four hours; this difference between balloon and nonballoon dogs is significant (P = .002). MAST did not have a statistically significant effect on survival. Perfusion pressure (PP) declined during the course of the experiment, and the balloon was effective at slowing this decline (P less than .0001); none of the other comparisons was statistically significant.     

Interplay between interferon-mediated innate immunity and porcine reproductive and respiratory syndrome virus

Innate immunity is the first line of defense against viral infection, and in turn, viruses have evolved to evade host immune surveillance. As a result, viruses may persist in host and develop chronic infections. Type I interferons (IFN-α/β) are among the most potent antiviral cytokines triggered by viral infections. Porcine reproductive and respiratory syndrome (PRRS) is a disease of pigs that is characterized by negligible induction of type I IFNs and viral persistence for an extended period. For IFN production, RIG-I/MDA5 and JAK-STAT pathways are two major signaling pathways, and recent studies indicate that PRRS virus is armed to modulate type I IFN responses during infection. This review describes the viral strategies for modulation of type I IFN responses. At least three non-structural proteins (Nsp1, Nsp2, and Nsp11) and a structural protein (N nucleocapsid protein) have been identified and characterized to play roles in the IFN suppression and NF-κB pathways. Nsp's are early proteins while N is a late protein, suggesting that additional signaling pathways may be involved in addition to the IFN pathway. The understanding of molecular bases for virus-mediated modulation of host innate immune signaling will help us design new generation vaccines and control PRRS.     

Disease Pandemics and Major Epidemics Arising From New Encounters between Indigenous Viruses and Introduced Crops

Virus disease pandemics and epidemics that occur in the world’s staple food crops pose a major threat to global food security, especially in developing countries with tropical or subtropical climates. Moreover, this threat is escalating rapidly due to increasing difficulties in controlling virus diseases as climate change accelerates and the need to feed the burgeoning global population escalates. One of the main causes of these pandemics and epidemics is the introduction to a new continent of food crops domesticated elsewhere, and their subsequent invasion by damaging virus diseases they never encountered before. This review focusses on providing historical and up-to-date information about pandemics and major epidemics initiated by spillover of indigenous viruses from infected alternative hosts into introduced crops. This spillover requires new encounters at the managed and natural vegetation interface. The principal virus disease pandemic examples described are two (cassava mosaic, cassava brown streak) that threaten food security in sub-Saharan Africa (SSA), and one (tomato yellow leaf curl) doing so globally. A further example describes a virus disease pandemic threatening a major plantation crop producing a vital food export for West Africa (cacao swollen shoot). Also described are two examples of major virus disease epidemics that threaten SSA’s food security (rice yellow mottle, groundnut rosette). In addition, brief accounts are provided of two major maize virus disease epidemics (maize streak in SSA, maize rough dwarf in Mediterranean and Middle Eastern regions), a major rice disease epidemic (rice hoja blanca in the Americas), and damaging tomato tospovirus and begomovirus disease epidemics of tomato that impair food security in different world regions. For each pandemic or major epidemic, the factors involved in driving its initial emergence, and its subsequent increase in importance and geographical distribution, are explained. Finally, clarification is provided over what needs to be done globally to achieve effective management of severe virus disease pandemics and epidemics initiated by spillover events.     

Barrett's Esophagus: Where Do We Stand?

Barrett''s esophagus (BE) is a precursor for esophageal adenocarcinoma, which has an increased incidence rate over the last few decades. Its importance stems from the poor five-year survival of esophageal adenocarcinoma and current data that suggest a survival benefit when surveillance programs are implemented. In this review, we will cover the pathophysiology and natural history of BE and the different endoscopic findings. The prevalence of BE in different geographic areas and the incidence of high-grade dysplasia and adenocarcinoma in this patient population is reviewed. Recent recommendation for screening and surveillance of BE has been covered in this review as well as the efficacy of nonconventional imaging modalities and endoscopic ablation therapies.     

Routine diagnosis of intestinal tuberculosis and Crohn’s disease in Southern India

AIM:To investigate whether routinely measured clinical variables could aid in differentiating intestinal tuberculosis(ITB)from Crohn’s disease(CD).METHODS:ITB and CD patients were prospectively included at four South Indian medical centres from October 2009 to July 2012.Routine investigations included case history,physical examination,blood biochemistry,ileocolonoscopy and histopathological examination of biopsies.Patients were followed-up after 2 and 6 mo of treatment.The diagnosis of ITB or CD was re-evaluated after 2 mo of antituberculous chemotherapy or immune suppressive therapy respectively,based on improvement in signs,symptoms and laboratory variables.This study was considered to be an exploratory analysis.Clinical,endoscopic and histopathological features recorded at the time of inclusion were subject to univariate analyses.Disease variables with sufficient number of recordings and P<0.05 were entered into logistic regression models,adjusted for known confounders.Finally,we calculated the odds ratios with respective confidence intervals for variables associated with either ITB or CD.RESULTS:This study included 38 ITB and 37 CD patients.Overall,ITB patients had the lowest body mass index(19.6 vs 22.7,P=0.01)and more commonly reported weight loss(73%vs 38%,P<0.01),watery diarrhoea(64%vs 33%,P=0.01)and rural domicile(58%vs 35%,P<0.05).Endoscopy typically showed mucosal nodularity(17/31 vs 2/37,P<0.01)and histopathology more frequently showed granulomas(10/30vs 2/35,P<0.01).The CD patients more frequently reported malaise(87%vs 64%,P=0.03),nausea(84%vs 56%,P=0.01),pain in the right lower abdominal quadrant on examination(90%vs 54%,P<0.01)and urban domicile(65%vs 42%,P<0.05).In CD,endoscopy typically showed involvement of multiple intestinal segments(27/37 vs 9/31,P<0.01).Using logistic regression analysis we found weight loss and nodularity of the mucosa were independently associated with ITB,with adjusted odds ratios of 8.6(95%CI:2.1-35.6)and 18.9(95%CI:3.5-102.8)respectively.Right lower abdominal quadrant pain on examination and involvement of≥3 intestinal segments were independently associated with CD with adjusted odds ratios of 10.1(95%CI:2.0-51.3)and 5.9(95%CI:1.7-20.6),respectively.CONCLUSION:Weight loss and mucosal nodularity were associated with ITB.Abdominal pain and excessive intestinal involvement were associated with CD.ITB and CD were equally common.     

Second and third line treatment options for Helicobacter pylori eradication

Helicobacter pylori is a highly successful bacterium with a high global prevalence and the infection carries significant disease burden. It is also becoming increasingly difficult to eradicate and the main reason for this is growing primary antibiotic resistance rates in a world where antibiotics are frequently prescribed and readily available. Despite knowing much more about the bacterium since its discovery, such as its genomic makeup and pathogenesis, we have seen declining treatment success. Therefore, clinicians today must be prepared to face one, two or even multiple treatment failures, and should be equipped with sufficient knowledge to decide on the appropriate salvage therapy when this happens. This article discusses the factors contributing to treatment failure and reviews the second and thirdline treatment strategies that have been investigated. Established empiric second line treatment options include both bismuth based quadruple therapy and levofloxacin based triple therapy. Antibiotic testing is recommended prior to initiating third line treatment. In the event that antibiotic susceptibility testing is unavailable, third line treatment options include rifabutin, rifaximin and sitafloxacin based therapies.     

岳阳市城区小学生血吸虫病防治健康教育现状调查

目的 调查湖南省岳阳市城区小学生血吸虫病防治知识、行为现状及健康教育需求情况,为制定科学有效的小学生血吸虫病健康教育方案提供参考依据。方法 采用分层整群抽样方法,抽取岳阳市城区洞庭湖湖畔学校和中心城区学校各2所,每所再从五、六年级分别抽取2个班的学生,通过调查问卷了解小学生血吸虫病相关知识、预防行为及健康教育需求,并进行统计分析。结果 共调查湖畔小学353人、中心城区小学363人,两组学校小学生年龄、性别、年级构成差异无统计学意义（t=-0.494,χ²_性别=1.615,χ²_年级=2.152;P均>0.05）。学生血吸虫病防治知识总知晓率为42.60%（305/716）,其中,湖畔学校小学生血防知识知晓率（52.97%）高于中心城区（32.51%）,差异有统计学意义（χ²=30.661,P<0.05）;学生行为正确率为76.68%（549/716）,血防知识知晓组行为正确率（81.31%）高于不知晓组（71.24%）,差异有统计学意义（χ²=6.384,P<0.05）。学生血防知识主要来源于老师（47.49%）;91.90%的学生愿意了解更多的血防知识,最喜欢的血防知识学习方式是参加课外活动（50.42%）。结论 岳阳市城区小学生的血防知识知晓率及行为正确率偏低,对血吸虫病防治健康教育需求较高。建议针对学生开展形式多样的血防健康教育,以增强学生的血吸虫病防护意识,提高自我防护能力。