雌激素受体、孕激素受体、血管内皮生长因子及其受体在子宫肌瘤中的表达和临床研究

目的:探讨子宫肌瘤的发生机制,检测子宫肌瘤组织中雌激素受体(ER)、孕激素受体(PR)和血管内皮生长因子(VEGF)及其受体(VEGFR1,VEGFR2)的阳性表达率,并探讨其临床意义.方法:应用免疫组织法(SP)测定30例子宫平滑肌瘤和相应子宫肌层组织的3种受体的表达.结果:子宫肌瘤ER阳性率为60.00%,PR阳性率为70.00%,相应子宫肌层组织ER、PR阳性率为40.00%、43.33%,两组ER和PR阳性率比较,差异有统计学意义(P＜0.05).子宫肌瘤组织中VEGF及VEGFR1、VEGFR2蛋白表达水平,无论在增殖期或分泌期,高于相应子宫肌层组织(P＜0.01);VEGFR2表达水平高于VEGFR1(P＜0.05).结论:ER、PR、VEGF均是反映子宫肌瘤的生物学行为的指标,研制ER、PR、VEGF及其受体蛋白的拮抗剂,使子宫肌瘤萎缩或凋亡,有望成为治疗子宫肌瘤新途径.     

PM_(2.5)暴露对小鼠卵巢雌、孕激素受体表达的影响

目的:通过小鼠PM_(2.5)染毒模型,观察小鼠卵巢组织病理变化及雌激素受体(ER)、孕激素受体(PR)表达的变化,探讨PM_(2.5)对卵巢的毒性作用。方法:将小鼠随机分为对照组、低剂量组(0.2592μg/μl)、中剂量组(1.56695μg/μl)和高剂量组(3.4560μg/μl),采用改良型气管滴注法,分别给予PBS或PM_(2.5)混悬液30μl/次,每3天1次,间断滴注7次。观察其染毒期间的精神、体重变化。染毒结束后于动情间期称量小鼠体重及卵巢重量,计算卵巢脏器系数;观察小鼠卵巢病理变化。免疫组化法检测卵巢ER、PR的分布,Western blot法检测卵巢ER、PR蛋白的表达变化。结果:与对照组比较,高剂量染毒组小鼠第14~18天体重减轻,差异有统计学意义(P0.05);中剂量组的卵巢湿重增加,差异有统计学意义(P0.05);高剂量组的卵巢湿重和卵巢脏器系数增加明显,差异均有统计学意义(P0.01,P0.05)。随着PM_(2.5)染毒剂量的增加,卵巢皮质变薄,结构混乱,间质纤维化,颗粒细胞排列较对照组紊乱,卵泡数减少,卵泡闭锁相对增多,黄体细胞明显增大变空,炎细胞浸润增多等。免疫组化染色结果示,高剂量组卵巢组织中ER、PR表达显著低于PBS对照组(P0.05)。Western blot法检测结果示,与对照组比较,随着染毒剂量的增加,卵巢中ER、PR蛋白表达逐渐降低,高剂量组中ER、PR蛋白表达均明显减少,差异有统计学意义(P0.05)。结论:随着PM_(2.5)暴露剂量增加,小鼠卵巢组织损害明显,提示暴露于高浓度的PM_(2.5)环境可损害卵巢组织结构,进而可能破坏卵巢功能。     

香芹酚拮抗大鼠卵巢储备化疗损伤的功能

目的探索香芹酚(CAR)对注射环磷酰胺(CTX)化疗损伤后大鼠卵巢功能的影响。方法 (1)将15只Fischer344大鼠随机分为3组,通过腹腔注射不同浓度CAR(10 mg/kg、50 mg/kg、100 mg/kg)在不同时间点利用化学发光法检测各组大鼠血清雌二醇(E_2)水平,通过酶联免疫吸附实验(ELISA)检测血清卵泡刺激素(FSH)水平。(2)64只Fischer344大鼠随机分为4组,分别为空白对照组、CAR组、CTX组和CTX+CAR组,每组16只,分别给予相应的药物后第60日和第90日,检测E_2和FSH水平,同时称量各组大鼠卵巢重量、计数卵泡数量以及卵泡直径,实时荧光定量PCR(q PCR)检测卵巢组织雌激素受体α(ERα)、雌激素受体β(ERβ)、孕激素受体(PR)和雄激素受体(AR)的表达。结果 50 mg/kg CAR组能有效降低E_2[(0.9±0.2)pg/m L]及FSH水平[(1.3±0.3)U/L],与10 mg/kg CAR组比较有统计学意义(P0.05),而与100 mg/kg CAR组无统计学差异(P0.05)。实验第60日质量[(38.5±4.1)mg]显著低于CTX+CAR组[(72.9±5.6)mg](P0.05),CTX组大鼠卵泡总数(1 250±95)显著低于CTX+CAR组(1 750±95),差异具有统计学意义(P0.05);此外,各组大鼠卵巢ERα、ERβ、PR以及AR基因表达无统计学差异(P0.05)。结论 CAR可通过降低E_2及FSH水平,提高卵巢重量和小卵泡数量而减轻CTX对大鼠卵巢功能的损伤。     

甲氨蝶呤不同方案治疗输卵管妊娠的疗效及副反应分析

目的　探讨甲氨蝶呤 (MTX)治疗输卵管妊娠的最佳剂量及给药途径。方法　回顾性分析 6 4 8例首选MTX治疗的未破裂输卵管妊娠 ,根据不同剂量及给药途径分为 6组 :MTX 10 0mg单剂量静脉推注 (组 1) ;MTX 10 0mg单剂量静脉推注 ,次日应用四氢叶酸 (组 2 ) ;MTX 10 0mg分 5d静脉推注 (组 3) ;MTX 10 0mg分 5d肌内注射 (组 4 ) ;MTX 75mg单剂量静脉推注 (组 5 ) ;MTX 75mg单剂量肌内注射 (组 6 )。比较 6组的重复给药率、成功率及副反应情况。结果　 (1)各组重复给药率、成功率比较 ,差异无显著性 (P >0 0 5 ) ,总成功率为 88 1% ,重复给药率为 2 3 1%。 (2 )肝丙氨酸转氨酶异常发生率 ,组 1至组 6分别为 10 3%、8 3%、6 4 3%、6 9 1%、8 7%、31 0 %。组 1、组 2、组 5低于其他各组 ,各组比较 ,差异均无显著性 (P均 <0 0 5 )。 (3)白细胞下降、口腔溃疡及呕吐发生率 ,各组均较低 ,各组比较 ,差异无显著性 (P >0 0 5 )。结论　MTX相同剂量、不同给药途径治疗输卵管妊娠的疗效相似 ;75mgMTX单剂量静脉推注的副反应发生率较低 ,在输卵管妊娠保守治疗时可作为首选方案。     

宫瘤消胶囊对子宫肌瘤模型大鼠雌孕激素水平及其受体表达的影响

目的探讨宫瘤消胶囊对子宫肌瘤模型大鼠雌、孕激素水平及雌激素受体(ER)、孕激素受体(PR)表达的影响。方法将SD大鼠随机分为正常对照组、模型组、宫瘤消小、中、大剂量组,各组10只。宫瘤消小、中、大剂量组各给予宫瘤消胶囊0.27 g/kg、0.54 g/kg和1.08 g/kg,除正常对照组外,其余各组采用雌、孕激素负荷法建立大鼠子宫肌瘤模型,分别连续灌胃4周。观察各组大鼠血清雌、孕激素水平及子宫肌层ER、PR的表达。结果宫瘤消小、中、大剂量组雌二醇水平分别为(321.49±23.40)pmol/L、(277.19±12.00)pmol/L、(259.90±12.60)pmol/L;孕酮水平分别为(87.51±16.05)nmo/L、(72.94±7.11)nmo/L、(66.04±9.11)nmo/L;ER表达率分别为(50.60±10.23)%、(23.61±7.46)%和(21.61±6.46)%,PR表达率分别为(59.28±7.37)%、(33.61±6.02)%和(30.61±6.46)%。其中宫瘤消小剂量组各指标与模型组比较,差异均无统计学意义(P0.05),而宫瘤消大剂量组各指标与模型组比较,差异均有统计学意义(P0.05)。结论宫瘤消胶囊可以降低雌二醇、孕酮水平,影响靶器官中ER、PR的表达,可能是宫瘤消胶囊抑瘤、缩瘤的机制之一。     

PRL-3抑制剂对子宫内膜异位症大鼠异位病灶的作用

目的:建立子宫内膜异位症动物模型,探索PRL-3抑制剂对SD大鼠异位病灶的影响。方法:选取36只雌性SD大鼠,将自体子宫内膜移植至腹壁造模,免疫组化实验鉴定病灶内膜的上皮及间质组织。内异症大鼠腹腔注射PRL-3抑制剂,按给药浓度分组:对照组(0mg/kg)、低剂量组(0.1mg/kg)、中剂量组(1mg/kg)和高剂量组(10mg/kg),比较处理前后各组病灶情况。结果:SD大鼠自体子宫内膜移植建模存活率为86.1%,存活大鼠的造模成功率为92.5%。SD大鼠各组处理后对比处理前,对照组病灶明显增大(P0.05),低剂量组和中剂量组病灶大小无明显变化,高剂量组病灶显著缩小(P0.05)。高剂量组的病灶显著小于对照组(P0.05)。结论:SD大鼠内异症模型可用于内异症的体内实验研究,是一种操作性强、重复性好且成本低的内异症模型。一定剂量的PRL-3抑制剂可抑制SD大鼠自体异位内膜病灶的生长;PRL-3抑制剂对内异症的靶向治疗有一定的潜在价值。     

DACH1在子宫内膜癌的表达及与ER、PR表达的相关性

目的:探讨细胞命运决定因子DACH1蛋白、ER、PR在子宫内膜癌的表达及分析其与子宫内膜癌临床病理指标及预后的关系。方法:免疫组化技术检测80例子宫内膜癌、20例正常子宫内膜、10例复杂增生子宫内膜和10例不典型增生子宫内膜组织标本中DACH1蛋白、ER、PR的表达,分析其与子宫内膜癌患者临床病理参数及预后的关系。结果:在子宫内膜癌、正常子宫内膜、不典型增生和复杂增生子宫内膜中DACH1蛋白表达的阳性率分别为36.3%(29/80)、95.0%(19/20)、8/10、10/10,DACH1蛋白在子宫内膜癌中的表达显著低于非癌组(P=0.000)。DACH1表达阳性率内膜癌Ⅰ期为47.7%,Ⅱ~Ⅳ期为22.2%,两者差异有统计学意义(P0.05)。DACH1表达阳性率与内膜癌的病理分级有关,G1~G2级显著高于G3级(43.6%vs 20.0%,P0.05)。内膜样癌中DACH1表达阳性率为42.2%,特殊类型癌中DACH1表达的阳性率为2/16,两者差异有统计学意义(P0.05)。单因素生存分析显示DACH1阳性组子宫内膜癌患者的总体生存率明显高于DACH1阴性组(P0.05)。80例子宫内膜癌组织中34例呈ER阳性表达,42例呈PR阳性表达,DACH1蛋白表达与ER蛋白表达呈正相关(ρ=0.245906,P0.05),与PR蛋白表达呈正相关(ρ=0.3527758,P0.01)。结论:DACH1蛋白在子宫内膜癌组织表达缺失,且和ER、PR表达有一定的相关性,它在子宫内膜癌的发生、发展及预后中可能起重要作用。     

着床窗期宫腔镜下子宫内膜形态与性激素及其受体表达的研究

目的:探讨着床窗期宫腔镜下子宫内膜形态与性激素及其受体表达的关系.方法:对55例不孕患者及16例正常生育者在排卵后7～9天,行宫腔镜子宫内膜形态的检查,并检测血雌二醇、孕酮及内膜雌激素受体(ER)、孕激素受体(PR).结果:不孕组宫腔镜下子宫内膜血管及腺体发育较对照组差,差异有统计学意义(P＜0.05).内膜差组腺上皮细胞ER表达较内膜佳组弱,差异有统计学意义(P＜0.05);内膜差组间质PR表达较内膜佳组弱,差异有统计学意义(P＜0.05).结论:不孕患者子宫内膜ER、PR的低表达,可能是导致子宫内膜腺体和血管网发育不良的重要原因之一.通过宫腔镜观察子宫内膜腺体和血管形态是一种较好的、简单可行的评估子宫内膜容受性的方法.     

雌/孕激素受体和HOXA-9基因在输卵管和异位妊娠输卵管中的表达

目的:探讨雌激素受体(ER)、孕激素受体(PR)和HOXA-9基因在人输卵管中的表达及与输卵管妊娠的关系。方法:采用免疫组化和原位杂交方法对34例正常输卵管和38例妊娠输卵管黏膜中ER、PR和HOXA-9基因表达进行研究。结果:ER、PR在正常增生期和分泌期输卵管黏膜上皮中的表达率较高;在妊娠输卵管黏膜上皮中的表达率明显下降(P<0.01)。HOXA-9基因在正常增生期输卵管中的表达率较高,分泌期输卵管中表达降低,而在妊娠输卵管中表达率又有所增高(高于分泌期输卵管的表达率,P<0.05)。结论:ER、PR在输卵管黏膜上皮中的表达下降和HOXA-9基因表达增强与输卵管妊娠密切相关;在正常分泌期输卵管黏膜上皮中,ER、PR表达水平上调和HOXA-9基因表达下调可能起着屏障作用,防止输卵管着床的发生。     

孕三烯酮对大鼠血清雌孕激素及在位和异位子宫内膜雌孕激素受体的影响

目的:探讨孕三烯酮(gestrinone)治疗子宫内膜异位症的作用机理。方法:用自体移植术建立大鼠子宫内膜异位症动物模型,随机分组给药,3周后剖腹,取血测定雌二醇(E2)、孕酮(P)浓度,取出子宫及移植物,固定、切片,免疫组化SP法测定各组动物子宫及移植物的雌激素受体(ER)和孕激素受体(PR)的表达。结果:孕三烯酮高剂量使大鼠血清E2、P水平降低,而中低剂量对E2、P水平影响不大。免疫组化结果则显示移植物的ER、PR含量较子宫内膜低,孕三烯酮高、中剂量可下调在位和异位子宫内膜ER、PR表达,而低剂量孕三烯酮则不能明显改变ER、PR的表达量。结论:孕三烯酮可通过降低体内雌孕激素水平以及下调在位及异位子宫内膜ER、PR的表达,发挥对子宫内膜异位症的治疗作用。     

Examination of the effects of methotrexate on histological and steroid receptor changes in the endosalpinx of the rat

Objectives

Methotrexate (MTX) has been a prevalent drug in conservative treatment of unruptured tubal pregnancy for many years. However, few researchers have performed morphological and protein analysis simultaneously to evaluate the specific toxic effects of MTX on the fallopian tubes. The aim of this study was to investigate acute and long-term toxic effects of increasing doses of MTX on the fallopian tubes and a possible dose–effect relationship. We also discuss the potential implications for subsequent pregnancies.

Study design

At the 10th day and the 2nd and 3rd months after MTX treatment, a total of 108 females SD rats in estrus stage (27 rats in each group) were collected according to the dose of MTX i.p.: 1, 2, 5 mg/kg body weight in groups I, II and III respectively and physiological saline i.p. in group IV for control. Nine rats in each group were killed at each time point and tissues close to the ampulla of the fallopian tubes were dissected for HE staining and routine histological observation. Estrogen receptor (ER) and progesterone receptor (PR) expression was detected by immunohistochemistry and Western blot.

Results

Morphological observation showed acute endosalpingitis in group I, becoming more intense with increasing doses of MTX in groups II and III in a reversible mode. Expression of ER in the endosalpinx significantly decreased in parallel with increasing doses of MTX in a dose–effect manner, which was reversible in groups I and II and irreversible in group III. Furthermore, ER and PR could recover close to normal levels in groups I and II after the 3rd month, while they could not restore to normal in group III.

Conclusions

These results provide the first evidence that MTX (≥5 mg/kg) can induce long-term, irreversible damage to steroid hormone receptors in the fallopian tubes in a dose-dependent manner. We tentatively suggest that MTX should be used in a relatively small and safe range of dosage in order to avoid impairment and potential risk of subsequent tubal pregnancy or infertility.     

Light and electron microscope examination of the effects of methotrexate on the endosalpinx

OBJECTIVE: To examine the effects of increasing doses of methotrexate (Mtx) on the fallopian tubes. STUDY DESIGN: The study was carried out on 24 female rats (Albino Wistar type, 250-300 g). The rats were randomly divided into four groups of six. Different doses of Mtx were given to the rats by i.p. injection: 1mg/kg to those in group 1, 5mg/kg in group 2 and 10 mg/kg in group 3. Rats in group 4 received injections of physiological serum only and were treated as the control group. Ten days after the injection, the fallopian tubes of the rats were removed for examination separately by light and electron microscopy (EM) for comparison. RESULTS: Light microscopy showed that in group 1 the surface epithelial cells were normal and the lamina propria was infiltrated by numerous inflammatory cells with a prevalence of polymorphonuclear leucocytes. Findings in groups 2 and 3 were similar: the lamina propria was infiltrated with granulocytes in one specimen from each of the two groups, and granulocytes were also observed among epithelial cells. In the control group all surface structures were found to be in a normal condition. Electron microscopy showed cilial loss in the epithelial cells and central crystolysis in mitochondria in all group 1 specimens. Findings in groups 2 and 3 were similar. The cytoplasm of the epithelial cells seemed to be dense, there was prominent crystolysis (crystalloid formation) in the mitochondria, and vacuolisation (vacuole formation) in the cytoplasm seemed to be augmented. Cilial loss was prominent, and the basal membrane was irregular. Epithelial cell nuclei were in disorder. Lipid granules were observed extensively in epithelial cells. Eosinophils seemed to be dominant in connective tissues below the epithelium. In all control group specimens the epithelium seemed to be normal with all organelles in place; the condition of intercellular junctions, ciliated epithelium and all mitochondria also seemed to be normal, and the basal membrane was observed to be in order. CONCLUSION: In view of these findings, we conclude that the ultrastructural derangements resulting from administration of Mtx in doses in excess of 1mg/kg can cause a reduction in the surface epithelium's ability to make rhythmic lashing movements and can impair the patency of the fallopian tubes. All these disturbances could be involved to some degree in the causation of infertility and recurrent ectopic pregnancy. Therefore, the dosage of Mtx should be limited to use of the lowest effective dose to avoid these adverse effects.     

Transvaginal intratubal methotrexate treatment of ectopic pregnancy.

OBJECTIVE: To evaluate the efficacy of transvaginal intratubal methotrexate (MTX) treatment of tubal ectopic pregnancy (EP). SETTING: Outpatient setting in University Hospital. PATIENTS: Forty women with early EP and rising serum beta-human chorionic gonadotropin (beta-hCG) levels. INTERVENTION: Transvaginal intratubal administration of MTX (1 mg/kg body weight). MAIN OUTCOME MEASURES: Success was defined as declining serum beta-hCG to undetectable levels, no tubal dilatation on ultrasound examination, and no further intervention was required. RESULTS: Treatment was associated with a 70% success rate. No difference was found in the success rate between women with an embryo (76.9%) and those with no embryo in their fallopian tubes (66.7%). The initial serum beta-hCG levels were also not different between patients who were successfully treated and those who failed to respond to the treatment. Despite declining serum beta-hCG levels, tubal rupture occurred in two patients. CONCLUSIONS: Treatment of EP by transvaginal MTX administration is associated with a 70% success rate. This is independent of the presence of an embryo or the initial serum beta-hCG levels. Rupture of EP can still occur despite low and declining serum beta-hCG levels.     

康普瑞汀磷酸钠对大鼠胚胎发育毒性的研究

目的:探讨抗肿瘤药物康普瑞汀磷酸钠(combretastatin A-4 disodium phosphate,CA4P)对SD孕鼠胚胎发育的毒性作用。方法:80只孕鼠随机分为低剂量(0.15 mg/kg)组、中剂量(0.50 mg/kg)组和高剂量(1.50 mg/kg)组及对照组(生理盐水),每组20只,给药容积均为10 m L/kg。于妊娠第6~15日尾静脉注射给药,每日1次。妊娠第20日解剖孕鼠,检查母体妊娠与胎鼠畸形情况。结果:与对照组比较,高剂量组的着床率、胚胎吸收率均有显著升高,活胎率降低,差异均有统计学意义(P0.05);妊娠后期高剂量组孕鼠体质量增长显著低于对照组,差异有统计学意义(P0.05);中、高剂量组的胎盘质量、活胎体质量、活胎顶臀长以及尾椎数、掌骨数、枕骨数、胸骨数均显著减少(P0.05);各剂量组未见胎鼠外观及内脏畸形。结论:本实验条件下,CA4P对亲代孕鼠未观察到不良反应的剂量水平(NOAEL)为0.50 mg/kg,对胚胎、胎仔发育的NOAEL为0.15 mg/kg,对胎仔致畸作用的NOAEL为1.50 mg/kg。     

氯化镉对大鼠睾丸超微结构的损伤

目的:观察氯化镉(CdCl2)对大鼠睾丸组织超微结构的损伤。方法:18只成年雄性SD大鼠随机均分为对照组(A组)与2个CdCl2实验组,实验组分别腹腔注射CdCl20.2mg/kg(B组)和0.8mg/kg(C组)2周。应用透射电镜观察睾丸超微结构的变化。结果:与对照组相比,B组和C组普遍观察到曲细精管基膜变薄,电子密度低,厚薄不均,呈波浪式皱褶;睾丸间质细胞、支持细胞、精原细胞分别有部分细胞出现胞质空泡,线粒体水肿、空泡化,内质网扩张、空泡化,溶酶体增多。C组部分精原细胞核固缩,少数精子部分顶体脱离细胞核。结论:CdCl2染毒可导致大鼠睾丸间质细胞、基膜、支持细胞、精原细胞和精子发生超微结构的改变,剂量增加时病理改变加重。     

两种剂量来曲唑对SD大鼠子宫内膜VEGF、IGF-Ⅰ的影响及意义

目的:研究两种剂量的来曲唑(LE)和克罗米酚(CC)对大鼠着床窗期子宫内膜血管内皮生长因子(VEGF)、胰岛素样生长因子-Ⅰ(IGF-Ⅰ)等子宫内膜容受性因子表达的影响及意义。方法:将48只清洁级SD大鼠随机分为空白对照组、5.2mg/kg CC组、0.26mg/kg LE组、0.52mg/kg LE组。免疫组化及RT-PCR技术检测各组大鼠子宫内膜VEGF、IGF-Ⅰ表达的强弱。结果:0.26mg/kg LE组、0.52mg/kg LE组大鼠子宫内膜VEGF、IGF-Ⅰ的表达与对照组差异无统计学意义;但CC组的子宫内膜VEGF及IGF-Ⅰ表达显著低于对照组,差异有统计学意义(P<0.01)。结论:0.26、0.52mg/kg LE不影响大鼠着床窗期VEGF、IGF-Ⅰ等容受性标志物的表达,而5.2mg/kg CC对大鼠子宫内膜容受性有一定的抑制作用。     

敌枯双和环磷酰胺对SD大鼠胚胎致畸作用比较

目的:寻找作为SD大鼠胚胎-胎仔发育毒性试验(Ⅱ段生殖毒性)较理想的阳性对照药物。方法:在大鼠胚胎-胎仔发育毒性试验中,分别在妊娠第10日给予不同剂量敌枯双和环磷酰胺,于妊娠第20日解剖观察胎仔畸形,从致畸种类、致畸率等指标分析比较致畸作用。结果:敌枯双11.0mg/kg组和13.8mg/kg组外观畸形率分别为84.62%和86.84%(P<0.01),而且种类较多,如:脊柱裂、心脏外露、尾畸形、肛门闭锁、骨骼畸形等;环磷酰胺组发生的畸形较少,以骨骼发育迟缓为主。结论:敌枯双对大鼠的致畸率高、致畸种类多,是较好的阳性对照药物。     

环境内分泌干扰物2-溴丙烷对大鼠睾丸毒性的研究

目的 :研究 2 -溴丙烷对大鼠睾丸的毒性。方法 :雄性成年 SD大鼠 40只 ,分为 1 80 0mg/ kg、6 0 0 mg/ kg、2 0 0 mg/ kg和对照组。腹腔注射 2 -溴丙烷 ,每日 1次 ,连续 5d。结果 :高剂量组大鼠体重下降 ,睾丸湿重和重量系数与对照组相比显著降低 ,而附属性腺的重量无显著改变。随着染毒剂量的增加 ,曲细精管损伤率升高 ( P<0 .0 5) ,精原细胞占曲细精管中生精细胞的比率显著降低 ,高剂量组大鼠的曲细精管面积减小。结论 :睾丸是 2 -溴丙烷生殖毒性的靶器官 ,2 -溴丙烷最早损伤精原细胞且毒作用最严重。     

L-肉碱在奥硝唑致雄性大鼠生殖系统氧化损伤中的作用

目的:探讨L-肉碱(LC)在奥硝唑(ORN)所致雄性大鼠生殖系统氧化损伤中的保护作用。方法:40只雄性SD大鼠随机均分为5组,分别连续灌胃20d。A组(对照组):0.5%羧甲基纤维素钠(溶剂);B组:400mg/kgORN;C组:800mg/kgORN;D组:ORN(400mg/kg)+LC(100mg/kg);E组:ORN(800mg/kg)+LC(100mg/kg)。末次给药24h后,麻醉处死所有大鼠,分别检测各组睾丸、附睾组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)水平及总抗氧化能力(TAC)。结果:B组、C组及E组大鼠附睾MDA水平明显高于A组(P均<0.01),睾丸中MDA水平则各组间没有差异;B组、C组及E组睾丸、附睾SOD水平和TAC则分别显著低于A组(P均<0.05),而D组与A组相比无显著性差异;与C组相比,E组大鼠附睾MDA水平显著降低(P<0.01),而TAC则显著升高(P<0.05);虽然E组大鼠附睾、睾丸SOD水平及睾丸中的TAC与C组比无统计学差异,但有升高的趋势。结论:LC可保护大鼠生殖系统对抗ORN所致的氧化损伤,对大鼠生殖功能具有一定的保护作用。