回收式自体输血在凶险性前置胎盘剖宫产术中的临床应用

目的:探讨回收式自体输血在凶险性前置胎盘患者剖宫产术中的应用效果。方法:对2016年1月至2016年12月在我院住院因凶险性前置胎盘行择期剖宫产术的70例患者进行回顾性分析,根据患者术中是否行回收式自体输血分为两组。剖宫产术中行回收式自体输血的患者36例为自体输血组,剖宫产术中未行回收式自体输血的患者34例为对照组。观察两组患者及新生儿的基本情况,术中出血及输血情况,围手术期血液学指标的变化。结果:自体输血组的术中出血量为1850 ml(700~7500 ml);对照组的术中出血量为2000 ml(700~12000 ml),两组比较差异无统计学意义(P0.05)。自体输血组中27.78%的患者术中输注异体血,对照组73.53%患者输注异体血,两组比较差异有统计学意义(P0.05)。自体输血组患者术中自体血回输量为666.5 ml(200~1788ml),自体输血组患者术后住院天数少于对照组,差异有统计学意义(P0.05)。两组患者围手术期血液检验指标比较,组间差异无统计学意义(P0.05),但自体输血组及对照组术前(24~72小时)与术后(24~48小时)的蛋白指标,包括总蛋白、白蛋白、球蛋白比较,术后均明显低于术前,且差异有统计学意义(P0.05)。两组均无输血不良反应。结论:凶险性前置胎盘患者剖宫产术中行回收式自体输血可有效减少患者围手术期异体输血比例及异体输血量,能有效避免同种异体输血带来的潜在风险,同时也节约了血源。     

妊娠期及哺乳期妇女跟骨骨密度的超声定量检测

为探讨妊娠期及哺乳期妇女骨密度的变化规律 ,我们采用MARCURY超声骨密度仪对孕 16～ 2 0周的孕妇 4 1例 (A组 ) ,孕 30～ 33周的孕妇 35例 (B组 )及产后 4 0～ 6 0d的哺乳期健康妇女 36例 (C组 )进行跟骨骨密度测定。结果显示 ,A组孕妇跟骨骨密度为 ( 0 5 3± 0 12 )g/cm2 ,B组孕妇跟骨骨密度为 ( 0 5 0± 0 0 8) g/cm2 ,C组妇女跟骨骨密度为( 0 4 6± 0 0 5 ) g/cm2 ,A、B两组比较 ,差异无显著性(P >0 0 5 ) ,C组与A、B两组明显降低 ,差异有显著性 (P<0 0 1)。超声传导速度 :A组为 ( 1837± 12 0 )m/s,B组为( 1835± 133…     

吗啡对雌性大鼠性腺轴和骨组织的影响

目的:研究吗啡对雌性大鼠性腺轴和骨组织的影响。方法:选取3月龄雌性大鼠45只,随机分为吗啡组30只和对照组15只。吗啡组采用剂量递增法皮下注射盐酸吗啡12周,对照组注射同等体积的生理盐水12周。放射免疫法测定血清FSH、LH、E2、P;免疫组织化学检测下丘脑、垂体、卵巢雌激素受体(ER)和β-内啡肽(β-EP)的表达;原位杂交方法测定下丘脑、垂体和卵巢的μ-阿片受体mRNA的表达;双能X线骨密度测量仪测量不同部位的骨密度值;测量骨代谢生化指标;对骨组织切片进行形态计量分析;并用RT-PCR方法检测骨组织中雌激素受体(ER)mRNA的变化。结果:(1)吗啡组FSH、LH、E2、P基础分泌较对照组降低(P<0·01,P<0·05,P<0·01,P<0·05);(2)吗啡组大鼠性腺轴各组织中ER平均光密度值均显著降低(P均<0·01);(3)吗啡组大鼠下丘脑、垂体β-内啡肽的含量下降,而μ-阿片受体mRNA表达增强;(4)吗啡组大鼠股骨远侧干骺端和胫骨近侧干骺端骨密度以及骨组织中ERmRNA表达均较对照组显著下降(P<0·05),组织切片观察显示,吗啡组大鼠骨小梁纤细、断裂、形态结构完整性差,骨髓腔大小不一,对照组大鼠骨小梁粗壮、饱满、形态结构完整,骨髓腔相对较小,计量分析显示,吗啡组骨小梁面积明显低于对照组(P<0·05);骨代谢生化指标结果显示,吗啡组大鼠血清钙和尿钙以及TRAP、HOP较对照组增加显著(P<0·05)。结论:长期使用吗啡对下丘脑-垂体-卵巢轴及骨组织会有不同程度的损伤。     

阿胶牡蛎口服液联合锌铁钙复合制剂对维生素D缺乏性佝偻病患儿骨代谢的影响

目的观察阿胶牡蛎口服液联合锌铁钙复合制剂治疗维生素D缺乏性佝偻病临床疗效及对患儿骨代谢的影响。方法选取2016年6月至2017年9月新疆医科大学第二附属医院预防保健科收治的维生素D缺乏性佝偻病患儿80例作为观察对象,随机分为观察组和对照组各40例。所有患儿均给予维生素D常规治疗,在此基础上,对照组给予锌铁钙复合制剂治疗,观察组给予阿胶牡蛎口服液联合锌铁钙复合制剂治疗。治疗12周后比较两组患儿临床疗效及骨代谢指标水平。结果治疗12周后,观察组患儿临床总有效率为95.0%(38/40),显著高于对照组77.5%(31/40),差异有统计学意义(P0.05)。骨代谢指标显示,两组治疗后桡骨、尺骨骨密度和25-(OH)D3水平显著高于治疗前,骨碱性磷酸酶水平显著低于治疗前,差异有统计学意义(P0.05)。治疗后观察组患儿桡骨、尺骨骨密度和25-(OH)D3水平显著高于对照组,骨碱性磷酸酶水平显著低于对照组,差异有统计学意义(P0.05)。结论在维生素D治疗基础上应用阿胶牡蛎口服液联合锌铁钙复合制剂治疗维生素D缺乏性佝偻病效果良好,能够提高临床疗效,并有效改善患儿骨代谢指标,临床上值得应用。     

缓释左旋-18甲基炔诺酮皮埋避孕剂对骨密度和骨代谢的影响

对采用 Norplant R埋植剂和仿制 Norplant的国产埋植剂 型避孕的育龄妇女骨密度和骨代谢改变进行了 1年的随机前瞻性临床观察。 6 1例正常妇女被分为两组 :Norplant埋植剂组 30例 ,国产埋植剂组 31例。两组于埋植前和埋植后第 12个月时采用双能 X线骨密度测定仪 (DEXA)分别测定了腰椎 L2 ～ L4、股骨近端骨密度和骨矿含量。两组妇女埋植后第 12个月时腰椎 L2 ～ L4骨密度和骨矿含量均较埋植前明显增加 (P<0 .0 1) ,Norplant组骨密度平均增加2 .40 % ,骨矿含量平均增加 3.34 % ,国产埋植剂组分别增加 2 .75 %和 4.47% ;从年龄分析 ,以 2 5～ 2 9岁组腰椎 L2 ～ L4骨密度埋植后增加最为显著 ,Norplant组 P<0 .0 5 ,国产埋植剂组 P<0 .0 1。国产埋植剂组妇女埋植后第 12个月时股骨大粗隆骨密度和骨矿含量较埋植前明显增加 (P<0 .0 1) ;国产埋植剂组妇女空腹尿羟脯氨酸 /肌酐比值埋植后第 12个月时较埋植前明显下降 (P<0 .0 1) ;对使用妇女腰椎、股骨骨密度和骨代谢生化指标的影响 ,在两种埋植剂之间埋植前后比较均无显著差别 (P>0 .0 5 )。左旋 - 18甲基炔诺酮皮埋避孕剂对绝经妇女的骨骼是非有害的 ,对年轻妇女骨峰值的获得无明显影响。     

非绝经期子宫切除保留卵巢对骨钙代谢影响的临床研究

目的　探讨子宫切除对骨钙代谢的影响以及术后预防措施。方法　 2 0 0 1年 1月至 2 0 0 2年 12月郑州铁路中心医院对 10 0例非绝经期子宫切除保留卵巢的患者 ,采用自身对照设计的方法进行前瞻性研究 ,分别于术前及术后 6个月进行骨密度检测。其中 70例术后为普通饮食 ,30例术后为高钙饮食。同时取条件相当的 10 0名正常女性作对照。结果　普通饮食组术后骨密度下降 5 2例 ,其中 12例发生骨质疏松 ;高钙饮食组术后骨密度下降 7例 ,高钙饮食组与普通饮食组术后比较 ,差异有显著性意义 (P <0 0 1)。结论　非绝经期保留双侧卵巢的子宫切除可使骨丢失加速 ,骨量减少 ,甚至发生骨质疏松症 ,这一情况可通过术后高钙饮食予以预防     

替勃龙上调卵巢切除大鼠腰椎护骨素mRNA的表达

目的:建立绝经后骨质疏松(postmenopausalosteoporosis,PMOP)的大鼠模型,研究替勃龙对卵巢切除大鼠腰椎组织中护骨素(OPG)基因mRNA表达水平的影响,探讨其预防和治疗PMOP的作用机制。方法:6月龄未交配健康雌性SD大鼠40只,随机分为SHAM组,OVX组,OVX+戊酸雌二醇(E2)组和OVX+替勃龙(tibolone,TIB)组。灌胃13周后处死动物,第四腰椎行骨组织形态计量指标测定,第二腰椎采用RT-PCR方法,对OPGmRNA表达水平进行检测。结果:OVX组大鼠较SHAM组TBV%显著下降;OSV%明显升高(P<0·05);E2和TIB均可使OVX大鼠的TBV%明显升高,OSV%明显下降;OPGmRNA表达水平在OVX大鼠组织中下调,与SHAM组相比有显著性差异(P<0.05),E2和TIB均可上调OVX大鼠骨组织OPGmRNA表达水平(P<0.05)。结论:E2和TIB均通过抑制骨转换预防和治疗PMOP;PMOP的发生与OPG有关,TIB和E2一样,其抗骨吸收效应很可能是通过OPG介导的。     

围生期孕妇骨量及骨代谢影响因素的临床分析

目的:探讨整个孕期影响骨代谢的相关因素.方法:选择在我院产科单胎足月分娩的健康产妇168名为调查对象,取入院待产后空腹血和晨尿,测定尿钙(Ca)、肌酐(Cr)、血碱性磷酸酶(ALP),产后3天内用双能X线骨密度仪测量左股骨颈骨密度.了解孕产史、母系骨质疏松家族史、孕期补钙及喝牛奶情况.结果:①发生骨量减少的产妇82例(48.8%),其中5例达到骨质疏松;②初产年龄及分娩前体重与左侧股骨颈骨密度值呈正相关(r=0.159,P=0.037);③发生骨量减少的孕妇中伴有母系骨质疏松家族史11例(73.3%),较骨量正常的孕妇伴母系骨质疏松家族史4例(26.7%)明显增多,差异有统计学意义(χ2=3.965,P=0.046);④持续补钙组孕妇左侧股骨颈骨密度高于未持续补钙组,骨形成指标血ALP、骨吸收指标尿Ca/Cr低于未持续补钙组,差异均有统计学意义(P<0.05).结论:孕期容易发生骨量减少,尤其对于伴有母系骨质疏松家族史的孕妇.孕期适当增加体重、持续补钙可以防治孕期骨质疏松,降低骨转换率.     

四环素-雌酮和雌酮作用去势大鼠的骨形态计量学对照研究

目的比较四环素－雌酮和雌酮对卵巢切除（ＯＶＸ）大鼠股骨远端骨干骨形态参数的影响。方法２０只雌性大鼠随机分成４组,每组５只：四环素－雌酮治疗组、雌酮治疗组、ＯＶＸ组和假手术组,建立ＯＶＸ大鼠骨质疏松动物模型,药物喂养１３周后处死,用骨形态计量学方法研究四环素－雌酮和雌酮对骨形态和动力学参数的影响。结果与假手术组相比,四环素－雌酮组和雌酮组骨小梁的连接性均明显地高于假手术组（Ｐ＜０．０５）。与ＯＶＸ组相比,四环素－雌酮和雌酮组四环素标记表面和类骨质表面均明显增加,四环素－雌酮组的结果又明显高于雌酮组,更明显高于其它两组（Ｐ＜０．０５）。结论四环素－雌酮和雌酮可以明显地加强骨小梁的连接性,四环素－雌酮提高骨激活频率的作用优于雌酮。     

Wnt/LRP5/β-catenin信号通路在绝经后骨质疏松发病中的作用

目的 探讨Wnt/LRPS/β-catenin信号通路在绝经后骨质疏松发病中的作用.方法 选取50只6月龄雌性Wistar大鼠,随机分为对照组(n=24)和去卵巢组(n=26),去卵巢组大鼠行双侧卵巢切除术,对照组打开大鼠腹腔后不作任何处理即缝合.去卵巢后0、4和8周分别测定两组大鼠血雌二醇水平和骨密度,4和8周用逆转录-PCR技术检测骨组织中低密度脂蛋白受体相关蛋白5( LRP5)、β连环蛋白(β-catenin)及骨形成关键基因—runt相关基因2(Runx2)mRNA的表达.结果 去卵巢组大鼠术后4和8周,出现雌二醇水平明显下降,术后4周时为( 92±15) pmol/L,对照组为( 117±29) pmol/L;术后8周时为(95±22) pmol/L,对照组为(114±15) pmol/L;两组分别比较,差异均有统计学意义(P＜0.05).术后4周时去卵巢组骨密度为(0.076±0.016) g/cm2,对照组为(0.098 +0.016) g/cm2;术后8周时去卵巢组为(0.052±0.013) g/cm2,对照组为(0.095±0.028)g/cm2;两组分别比较,差异均有统计学意义(P＜0.01).去卵巢组大鼠术后4周骨组织中LRP5、β-catenin和Runx2 mRNA表达水平明显减少,分别为0.97±0.04、0.58±0.05、0.86±0.03,对照组为1.02±0.06、1.04±0.05、1.07±0.21,两组各指标分别比较,差异均有统计学意义(P＜0.05).结论 Wnt/LRP5/β-catenin信号通路抑制可能是绝经后骨质疏松重要发病机制之一.     

Allogeneic and autologous ovarian orthotopic transplantation without a vascular pedicle: morphological, endocrinologic and natural pregnancy assessment

BACKGROUND: To assess the natural pregnancy of rabbits submitted to bilateral oophorectomy and orthotopic allogeneic or autologous intact and sliced ovarian transplantation without a vascular pedicle, and to determine the morpho-functional aspects of the transplanted ovaries. METHODS: Fifty-six female rabbits were studied. The ovaries were removed and orthotopically transplanted or replaced without vascular anastomoses: Group 1 (n = 8), only laparotomy and laparorrhaphy; Group 2A (n = 8), intact ovaries were transplanted on both sides; Group 2B (n = 8), both ovaries were sliced and orthotopically transplanted; Group 2C (n = 8), an intact ovary was transplanted on one side and a sliced ovary on the other side; Groups 3A-C, the ovaries were reimplanted according to the procedure and subgroups described for Group 2. Three months later, the animals were paired with males for copulation. Estradiol, progesterone, FSH and LH hormone levels were assessed. A histological study was carried out, and the number of pregnancies and litters were also determined. Chi-squared test compared the number of pregnancies and litters. One-way anova and the Tukey-Kramer tests compared the hormonal dosages. RESULTS: Pregnancies occurred in seven rabbits of Group 1, in 37.5% of rabbits in Groups 2A and 3A, in 50% of groups 2B, 3B and 3C, and in 62.5% of group 2C. Hormone levels and histology confirmed the vitality of all ovaries. CONCLUSIONS: Intact or sliced orthotopic allogeneic and autologous ovarian transplantation without a vascular pedicle is viable in rabbits, and preserves their hormonal and fertile functions.     

聚乳酸基形状记忆材料对兔输卵管黏膜的影响

目的:探讨聚乳酸基形状记忆输卵管避孕材料的安全性。方法:雌性大白兔15只随机分为实验组、自身对照组及空白组,分别于术后1、4、8周,取输卵管制作标本,以光镜及透射电镜观察黏膜变化。结果:实验组及自身对照组手术侧,肉眼可见材料植入体内后变短粗,后期逐渐变细小,光镜下初期可见输卵管皱襞被压缩,后皱襞内逐渐有纤维增生。电镜下上皮细胞内有异物颗粒,后期肌细胞间可见大量胶原。结论:聚乳酸基形状记忆材料植入兔输卵管后能刺激输卵管纤维组织增生,材料可降解,对机体无危害。     

Successful fetal surgery for the repair of a 'myelomeningocele-like' defect created in the fetal rabbit

OBJECTIVE: To study the correction of a 'myelomeningocele-like' defect in fetal rabbits. METHODS: Twelve pregnant rabbits had a spinal defect surgically created in 40 of their fetuses at 23 days of gestation. Immediate repair was performed in 30 fetuses (group I), and 10 remained uncorrected (group II). After 30 days, the fetuses were harvested and the anatomopathological aspects where compared using Fisher's exact test. RESULTS: Three different techniques to apply a cellulose graft were used for correction in 8 (technique A), 7 (technique B), and 15 animals (technique C), but only one (technique C) was successful. The survival rate at 30 days was 66.7% (10/15) in group I and 80% (8/10) in group II. A 'myelomeningocele-like' defect was present in all fetuses in group II, while in group I the defect was successfully repaired in 80% of the surviving fetuses (p < 0.01). CONCLUSION: The surgically created spinal defect was successfully repaired, and also the fetal rabbit could be established as a model for the study of intrauterine correction of a myelomeningocele-like defect.     

尼尔雌醇作用下去势雌兔骨组织形态计量学改变

目的：评估尼尔雌醇对绝经后骨质疏松症的疗效，并探讨其机理。方法：将３０只雌性新西兰兔随机分成３组（每组１０只）：治疗组［摘除卵巢＋尼尔雌醇（每月２ｍｇ）］、模型组（摘除卵巢）和正常对照组（假手术），６个月后处死取材，并在处死前行四环素标记，ＶＩＤＡＳ图象分析系统下作骨组织形态计量学测定。结果：治疗组的单位体积骨小梁骨量（ＢＶ／ＴＶ）、相对类骨质量（ＯＶ／ＢＶ）、成骨细胞指数（ＯＢＩ）和组织水平的骨形成速率［ＢＦＲ（Ｔ）］明显高于模型组和（或）正常对照组。结论：尼尔雌醇能增加成骨细胞数量，可能是一种成骨细胞募集和（或）增殖的刺激剂。     

Three-dimensional cephalometric evaluation of maxillary growth following in utero repair of cleft lip and alveolar-like defects in the mid-gestational sheep model

OBJECTIVE: To evaluate maxillary growth following in utero repair of surgically created cleft lip and alveolar (CLA)-like defects by means of three-dimensional (3D) computer tomographic (CT) cephalometric analysis in the mid-gestational sheep model. METHODS: In 12 sheep fetuses a unilateral CLA-like defect was created in utero (untreated control group: 4 fetuses). Four different bone grafts were used for the alveolar defect closure. After euthanasia, CT scans of the skulls of the fetuses, 3D reconstructions, and a 3D-CT cephalometric analysis were performed. RESULTS: The comparisons between the operated and nonoperated skull sides as well as of the maxillary asymmetry among the experimental groups revealed no statistically significant differences of the 12 variables used. CONCLUSIONS: None of the surgical approaches used for the in utero correction of CLA-like defects seem to affect significantly postsurgical maxillary growth; however, when bone graft healing takes place, a tendency for almost normal maxillary growth can be observed.     

The influence of dehydroepiandrosterone on histology of selected organs in rabbits on an atherogenic diet.

The influence of dehydroepiandrosterone (DHEA) in fodder on the histology of selected organs in rabbits with induced hypercholesterolemia and in healthy rabbits was studied. Rabbits were randomly assigned into four groups: (1) control; (2) atherogenic diet; (3) atherogenic diet with addition of DHEA; (4) normal diet with addition of DHEA. After 12 weeks, the rabbits were bled. Tissue samples were collected, fixed in a 0.4% solution of buffered formalin, dehydrated and embedded in paraffin. Fragments of 5-7 microns were stained with hematoxylin and eosin as well as according to the van Gieson method. Histological analysis showed features of steatosis and intense degenerative changes in analyzed organs of animals from group 2, i.e. liver, kidneys, adrenal glands, lungs and bone. The degenerative changes in the group which in addition to a fat-rich diet received DHEA, were similar to group 2, but much less intense. Histological pictures of organs of the rabbits which received DHEA and normal diet did not differ significantly from the control group. In animals with experimental hyperlipidemia, DHEA acts protectively, decreasing degenerative changes in internal organs caused by an atherogenic diet. DHEA does not change the histological picture of organs in healthy animals.     

A different technique to create a 'myelomeningocele-like' defect in the fetal rabbit

OBJECTIVE: To evaluate the fetal survival rate using a modified technique to surgically create a 'myelomeningocele-like' defect in a rabbit model. METHODS: Six white New Zealand rabbits had a spinal defect created in their fetuses at 23 days of gestation. At 30 days of gestation, the fetuses were harvested for anatomo-pathologic evaluation. RESULTS: The defect was created in 19 fetuses and an attempt to correct it was made in 15 cases (group I), and 4 fetuses where left without correction (group II). At 30 days, a 'myelomeningocele-like' defect was present in all surviving fetuses. The total survival rate was 73.68% (14/19); 11 fetus survived in group I and 3 in group II. CONCLUSION: The technical modifications, including fetal positioning and exposure of its back prior to the incision of the lamina, associated with a different technique for myometrium closure, offer an alternative and probably safer method to surgically create a spinal defect in the fetal rabbit.     

对妊娠中晚期孕妇行超声检测胎儿鼻骨发育状况以筛查唐氏综合征

目的 探讨超声检测胎儿鼻骨发育状况对产前筛查唐氏综合征的临床应用价值.方法 2004年10月至2007年3月,应用二维超声对中山大学附属第一医院1863例中晚期孕妇(正常组)和25例妊娠唐氏综合征胎儿的孕妇(研究组)检测其胎儿鼻骨,观察两组中正常胎儿和唐氏综合征胎儿的鼻骨缺失、鼻骨短小的发生率.鼻骨缺失为胎儿面部矢状切面、横切面和冠状切面均未能显示鼻骨声像;鼻骨短小为鼻骨超声测值小于同孕周正常值的第2.5百分位数.计算鼻骨发育异常值筛查唐氏综合征的诊断试验结果.结果 (1)正常组孕妇中成功检测胎儿鼻骨1761例,102例受胎位等因素影响未能得到清晰图像,检测成功率为94.5%(1761/1863);(2)正常组中1761例胎儿鼻骨长度与孕周呈正相关关系(r=0.605,P＜0.05),其中鼻骨缺失3例(0.2%,3/1761),鼻骨短小44例(2.5%,44/1761);(3)研究组唐氏综合征胎儿中,鼻骨缺失7例(28.0%,7/25),鼻骨短小15例(60.0%,15/25);(4)以鼻骨缺失为截断值筛查唐氏综合征的敏感度为28.O%,特异度为99.8%,阳性似然比164.45(95%可信区间为45.11～599.60),阴性似然比0.72(95%可信区间为0.57～0.92);以鼻骨短小为截断值筛查唐氏综合征的敏感度为60.0%,特异度为97.5%,阳性似然比24.03(95%可信区间为7.15～80.71),阴性似然比0.41(95%可信区间为0.29～0.59).结论 胎儿鼻骨发育异常尤其是鼻骨缺失,与唐氏综合征关系密切,可作为产前超声筛查唐氏综合征的指标应用于临床.     

Morphologic,endocrinologic and natural pregnancy assessment of allogeneic ovarian orthotopic transplantation without a vascular pedicle in rabbits

Objective

The objective was to assess the natural pregnancy of rabbits subjected to bilateral oophorectomy and orthotopic allogeneic intact and sliced ovarian transplantation without a vascular pedicle, and to determine the morphofunctional aspects of the transplanted ovaries.

Study design

Thirty-two female rabbits were studied. The ovaries were removed and orthotopically transplanted without vascular anastomoses between the two breeds of rabbits. In Group 1 (n = 8), only laparotomy and laparorrhaphy were performed, in Group 2A (n = 8) intact ovaries were transplanted on both sides, in Group 2B (n = 8) both ovaries were sliced and orthotopically transplanted, and in Group 2C (n = 8) an intact ovary was transplanted on one side and a sliced ovary on the other side. Three months later, the animals were paired with males for copulation. Estradiol, progesterone, follicle-stimulating hormone, and luteinizing hormone levels were assessed. A histologic study was carried out and the numbers of pregnancies and litters were also determined.

Results

Pregnancies occurred in seven rabbits in Group 1, 37.5% of rabbits in Group 2A, 50% in Group 2B, and 62.5% in Group 2C. Hormone levels and histology confirmed the vitality and function of all ovaries.

Conclusions

Intact or sliced orthotopic allogeneic ovarian transplantation without a vascular pedicle is viable in rabbits, and preserves their hormonal and fertile functions.     

模拟子宫内膜微环境体外诱导兔骨髓间充质干细胞向子宫内膜上皮细胞分化的实验研究

目的:探讨模拟子宫内膜微环境体外诱导兔骨髓间充质干细胞(BMSCs)向子宫内膜上皮细胞方向分化的可行性。方法:原代培养兔BMSCs并鉴定,提取子宫内膜条件培养液,用子宫内膜条件培养液和雌激素(β-雌二醇,1×10-7mol/L)体外诱导分化BMSCs,用细胞免疫荧光法和Western blot检测分化的BMSCs是否表达上皮细胞特异性标记蛋白。结果:兔BMSCs具有较强的增殖潜能,呈克隆性生长,表达CD44和CD90,表达率分别为99.91%、98.64%;不表达CD45。子宫内膜条件培养液和雌激素培养BMSCs5天后,大部分细胞形态由间质细胞向上皮样细胞分化;细胞免疫荧光鉴定诱导分化后的BMSCs表达角蛋白;Western blot检测实验组角蛋白表达量明显增加,与对照组相比有统计学差异(P<0.05)。结论:子宫内膜条件培养液联合雌激素可以诱导兔BMSCs向子宫内膜上皮细胞方向分化。体外模拟的子宫内膜微环境在BMSCs向子宫内膜上皮细胞分化中起重要作用。