人参蛤蟆油胶囊细胞免疫调节作用的实验研究

目的探讨人参蛤蟆油胶囊对机体的细胞免疫调节作用。方法实验组分别给予0.05、0.50、1.50 g/kgBW的人参蛤蟆油胶囊,同时设空白对照组(给大豆油),测定足跖肿胀度和淋巴细胞转化程度。结果人参蛤蟆油胶囊能增强小鼠的迟发型变态反应,是否肿胀度达到0.75 mm,提高ConA诱导的小鼠脾淋巴细胞转化能力OD差值达到0.173。结论人参蛤蟆油胶囊具有增强细胞免疫调节作用。     

三氯乙烯染毒小鼠免疫功能变化研究

目的观察三氯乙烯对NIH小鼠染毒后免疫功能的变化.方法给予NIH小鼠皮下三氯乙烯染毒后研究胸腺、脾、肝脏等脏器系数变化情况、进行足跖肿胀实验、脾细胞转化实验等.结果三氯乙烯染毒小鼠胸腺、脾脏器系数未见明显变化.24h、48h时小鼠足跖肿胀系数明显高于溶剂对照组.80 mg/L三氯乙烯可抑制溶剂对照组脾淋巴细胞的增殖,但可促进三氟乙烯染毒组脾淋巴细胞增殖.结论三氯乙烯可能诱导NIH小鼠产生迟发性变态反应.     

食品级番茄红素对小鼠的免疫调节功能

目的研究番茄红素对免疫系统的调节作用。方法实验小鼠随机分成3组：对照组、低剂量组（10mg／kg）、高剂量组（20mg／kg）。低剂量组、高剂量组分别用色拉油稀释的番茄红素灌胃，对照组用色拉油灌胃。30天后检测小鼠的脾淋巴细胞增殖程度、NK细胞的杀伤活性、迟发性变态反应的程度、巨噬细胞吞噬功能。结果与对照组比较，高剂量组能显著增强脾淋巴细胞的增殖能力，提高自然杀伤细胞的活性和巨噬细胞吞噬功能，增强小鼠的足跖肿胀度（P〈0．05）。除增殖反应外，低剂量组其他指标与对照组比较，差异均无显著性。结论番茄红素具有增强小鼠免疫调节的功能。     

人参天麻蜂王浆口服液免疫作用的实验研究

[目的]了解人参天麻蜂王浆口服液对小鼠免疫功能的影响。[方法]经连续经口给药30d，对小鼠进行碳廓清能力、迟发型变态反应、血清溶血素、抗体生成细胞、脾淋巴细胞增殖转化能力、NK细胞活性和腹腔巨噬细胞吞噬鸡红细胞等指标的测定。[结果]低剂量组小鼠胸腺指数高于对照组；高剂量组能提高二硝基苯氟苯（DNFB）诱导的小鼠迟发型变态反应能力，增强小鼠脾淋巴细胞的增殖转化能力，提高血清溶血素的水平，促进抗体生成细胞的生成，提高小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬指数及增强NK细胞的活性；而对小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬率、碳廓清吞噬指数均无影响。[结论]人参天麻蜂王浆口服液的功效成分10-羟基-2-癸烯酸和人参皂甙是较强的免疫调节功效因子，具有增强小鼠免疫功能的作用。     

杜仲叶茶功效成分对小鼠免疫功能影响

目的研究以杜仲叶为原料制成的绿茶对小鼠免疫功能的影响。方法杜仲叶茶中绿原酸和总黄酮含量分别用高效液相色谱法和分光光度法测定。小鼠细胞免疫功能测定：迟发型变态反应（DTH）实验采用绵羊红细胞（SRBC）诱导小鼠DTH（足跖增厚法）,刀豆蛋白A（ConA）诱导的小鼠脾淋巴细胞转化试验,用四甲基偶氮唑盐微量酶反应比色法（MTT法）。小鼠体液免疫功能的测定;用血凝法测定血清溶血素,Jerne改良玻片法检测抗体生成细胞。小鼠碳廓清试验法测定单核-巨噬细胞功能：用半体内法进行小鼠腹腔巨噬细胞噬鸡红细胞试验。用乳酸脱氢酶（LDH）测定法进行天然杀伤细胞（NK细胞）活性测定。结果杜仲叶茶中绿原酸含量为1.20g/100g,总黄酮含量（以芦丁计）为465mg/100g,经口给予小鼠不同剂量的杜仲叶茶30d后,与对照组比较,杜仲叶茶能增加小鼠的足跖肿胀度,提高小鼠的淋巴细胞增殖能力,增强小鼠的腹腔巨噬细胞吞噬鸡红细胞的能力和小鼠碳廓清吞噬指数（P〈0.05）;各剂量组小鼠的胸腺/体重比值、脾脏/体重比值、小鼠血清溶血素和NK细胞活性转换值的测定结果与对照组比较,差异均无统计学意义（P〉0.05）。结论杜仲叶茶对小鼠的细胞免疫功能和单核-巨噬细胞功能均具有明显的增强作用,而对小鼠的体液免疫功能未见明显影响。     

沙棘全果油对小鼠免疫功能调节作用的实验研究

目的评价沙棘全果油对昆明种小鼠的免疫调节作用。方法将小鼠适应性喂养到一定体重后随机分为4组,每组40只共160只,按照受试物低、中、高、阴性对照4个不同浓度进行30 d灌胃。其中免疫1组进行巨噬细胞吞噬率和吞噬指数测定试验;免疫2组进行淋巴细胞增殖能力测定和NK细胞活性测定;免疫3组进行溶血空斑数、抗体水平积数测定和足跖肿胀度测定;免疫4组进行碳廓清能力试验。实验结束进行体重和脏器的测定及脏器/体重比值的计算,对各免疫小组的试验数据根据不同灌胃剂量进行方差分析或秩和检验。结果 4个免疫组不同灌胃剂量的动物在试验中期、末期的体重相比较差异无统计学意义,脾脏/体重比值和胸腺/体重比值分别与阴性对照组相比,均无显著性差异。单核-巨噬细胞吞噬功能试验、淋巴细胞转化试验、体液免疫试验和碳廓清能力试验结果均为阴性,差异无统计学意义。足趾肿胀试验和NK细胞活性试验结果为阳性。结论沙棘全果油对小鼠具有增强免疫力的功能。     

北京蜂胶对小鼠免疫功能影响的实验研究

目的探讨北京蜂胶对正常小鼠免疫功能的影响。方法采用0.183、0.367、1.101g/kg.bw剂量的北京蜂胶给小鼠经口灌胃30d,分别测定免疫器官脏器/体重比值,进行迟发型变态反应(DTH)(足跖增厚法)、ConA诱导的小鼠淋巴细胞转化实验(MTT法)、抗体生成细胞检测(Jerne改良玻片法)、半数溶血值(HC50)测定和小鼠碳廓清实验、NK细胞活性的测定(同位素3H-TdR测定法)。结果与阴性对照组比较,0.367、1.101g/kg.bw剂量能明显提高小鼠的迟发型变态反应的能力,1.101g/kg.bw剂量能明显提高小鼠抗体生成细胞数、半数溶血值,明显增强小鼠单核—巨噬细胞碳廓清能力,明显提高小鼠NK细胞活性。三个剂量对小鼠体重增长、胸腺体重比值、脾脏体重比值、ConA诱导的小鼠淋巴细胞转化能力均无明显影响。结论北京蜂胶具有增强免疫力作用。     

龟鹿口服液对小鼠免疫功能影响的实验研究

目的　了解龟鹿口服液对小鼠免疫功能的影响。方法　采用雌性昆明种小鼠经口连续给予 2 5、5 0、15 0ml/kg·bw的龟鹿口服液 30d ,进行迟发型变态反应 (足跖厚度增加法 )、小鼠脾淋巴细胞转化试验、溶血素滴度测定、抗体生成细胞检测等试验。结果　受试物对小鼠的体重、脾脏和胸腺重量无明显影响 (P >0 0 5 ) ;对小鼠迟发型变态反应试验中 ,各剂量组足跖增厚值均高于对照组 ;对ConA诱导的小鼠脾淋巴细胞转化试验 ,低剂量组吸光度差值高于对照组 ;对受试动物血清溶血素抗体滴度水平的影响 ,中、高剂量组的抗体积数高于对照组 ;对受试动物脾细胞抗体生成水平的影响 ,各剂量组的脾细胞抗体生成水平 (空斑数 )均高于对照组 ,差异均有显著性 (均P <0 0 5 )。对小鼠碳廓清吞噬功能和对小鼠腹腔巨噬细胞吞噬鸡红细胞吞噬功能以及NK细胞活性测定中 ,各剂量组与对照组比较 ,差异均无显著性 (均P >0 0 5 )。结论　龟鹿口服液具有增强小鼠细胞和体液免疫功能的作用。     

某冲剂抗衰老和免疫调节作用的研究

目的 观察以大豆磷脂、维生素E、低聚果糖为主要成分的某冲剂对果蝇寿命,小鼠抗氧化及免疫功能的影响。方法 果蝇生存试验,观察含不同浓度受试物培养基中生长的果蝇的生存时间,计算平均寿命、平均最高寿命和半数死亡时间;抗氧化试验;给予不同剂量受试物30天后测定老龄小鼠血清中脂质过氧化降解产物－丙二醛（MDA）和含量和超氧化物歧化酶（SOD）的活力;免疫功能试验;分别以足跖增厚法、Jerne改良玻片法、碳廓清试验测定小鼠的细胞免疫、体液免疫及巨噬细胞吞噬功能。结果 该冲剂能显著延长果蝇的平均寿命和平均最高寿命、降低小鼠血清MDA含量,提高血清SOD活力,能显著增强小鼠的足跖肿胀度,显著增加小鼠抗体生成细胞数,显著提高小鼠的碳廓清能力。     

某蛋白粉增强免疫功能的研究

目的评价某蛋白质粉对小鼠免疫功能的影响。方法按《保健食品检验与评价技术规范》中增强免疫力功能检验方法,经口给予小鼠0.83、1.67、5.0g/kg·bw剂量的蛋白质粉30d,测定细胞免疫、体液免疫、单核-巨噬细胞吞噬功能指标,以及NK细胞活性。结果高剂量组足跖肿胀度为(3.05±0.12)mm,淋巴细胞增殖能力[OD差值为(0.127±0.034)],溶血空斑数(×10~3/全脾)为(159.90±50.17),抗体积数为(188.31±39.73),均显著高于正常对照组(P0.05),实验组能显著提高小鼠淋巴细胞转化能力、迟发型变态反应能力、血清溶血素水平和抗体生成细胞数。各剂量组对小鼠体重增长、胸腺/体重比值、脾脏/体重比值、单核一巨噬细胞碳廓清能力、巨噬细胞吞噬鸡红细胞的能力、NK细胞活性均无显著影响(P0.05)。结论蛋白质粉具有增强免疫力的功能。     

牛初乳的免疫调节作用

目的验证牛初乳的免疫调节作用。方法根据卫生部保健食品功能学评价程序和检验方法的要求,对完达山牌牛初乳进行了迟发型变态反应、半数溶血值测定实验、碳廓清实验。结果①在迟发型变态反应中,高剂量组24h后的足跖肿胀度与普通奶粉对照组间差异有统计学意义(P0.01)。②在半数溶血值测定实验中,高剂量组与普通奶粉对照组的半数溶血值差异有统计学意义(P0.01)。③在碳廓清实验中,高、中剂量组的吞噬指数与普通奶粉对照组比较,差异有统计学意义(P0.05)。结论牛初乳能增强小鼠的细胞免疫、体液免疫、单核-巨噬细胞的吞噬功能,即具有免疫调节作用。     

All-trans retinoic acid biases immune response induced by DNA vaccine in a Th2 direction

Vitamin A deficiency diminishes Th2-mediated Ab responses. Providing Vitamin A or its active metabolites reverses this defect. All-trans retinoic acid (ATRA), an acid derivation of Vitamin A, regulates the balance of immune response induced by TR421-hCGbeta DNA vaccine. Compared to DNA vaccine alone or treatment with vehicle, significantly higher level of antibody against the protein encoded by DNA vaccine was observed in mice 6 weeks after the first immunization. The IgG2a/IgG1 ratio was lower in mice treated with ATRA. We also found that treatment with ATRA also diminishes specific cellular immune response induced by gene immunization by measuring the marker of cellular immune response. We conclude that ATRA biases the immune response to Th2 direction induced by DNA vaccine and acts as a candidate adjuvant and immunomodulatory molecule.     

低聚半乳糖对小鼠免疫功能的影响

目的研究低聚半乳糖对小鼠免疫功能的影响。方法昆明种小鼠随机分为低、中、高3个剂量组和1个阴性对照组,剂量组动物分别灌胃给予83.3、833和2500mg/kg体重的低聚半乳糖,阴性对照组给予去离子水,连续灌胃30d后,对小鼠进行细胞免疫指标、体液免疫指标、巨噬细胞吞噬功能及NK细胞活性测定。结果中、高剂量组小鼠足跖肿胀度值和血清半数溶血值(HC50)明显高于阴性对照组(P<0.01),低、中剂量组小鼠碳廓清指数明显高于阴性对照组(P<0.01)。结论在该试验条件下,低聚半乳糖对小鼠具有免疫增强作用。     

Chemotypical variations in Withania somnifera lead to differentially modulated immune response in BALB/c mice

Withania somnifera (Ashwagandha) is a plant with known ethnomedicinal properties and its use in Ayurvedic medicine in India is well documented. The present investigation reports on immunomodulatory efficacy of aqueous-ethanol extracts of roots of three selected Withania somnifera chemotypes designated as NMITLI 101R, NMITLI 118R and NMITLI 128R. Each chemotype was administered 10-100 mg/kg orally to BALB/c mice once daily for 14 days. The immunomodulatory consequences were recorded by determining the humoral immune response with the help of hemagglutination, plaque forming cell assay and cellular response by measuring delayed type hypersensitivity reaction. Additionally, other immune parameters such as proliferation of T and B cells, intracellular and secreted Th1 and Th2 cytokines along with modulation in ROS production by peritoneal macrophages were monitored after feeding with lower doses (3-30 mg/kg/day) of these three chemotypes individually. NMITLI 101R incited both humoral and cellular immune response in terms of higher number of antibody producing cells and enhanced foot pad swelling at the 10 mg dose as also dose dependent B and T cell proliferations. Levels of intracellular and secreted cytokines post-NMITLI 101R treatment illustrated generation of mixed Th1/Th2 response that remained more polarized towards Th1. This chemotype also generated maximum reactive oxygen species. NMITLI 118R provoked comparatively reduced immune response in all humoral and cellular parameters at lower doses but induced highly polarized Th1 cytokine response. In contrast, NMITLI 128R led to enhanced antibody production with minimal cellular response demonstrating marginally Th2 dominance at a lower dose. Taken together, it may therefore be concluded that there were distinct modulation in the immune response exhibited by the three chemotypes of Withania somnifera and NMITLI 101R appeared to possess a better immunostimulatory activity than the other chemotypes at lower doses.     

人参皂苷Rg1对运动性疲劳小鼠脑氨基酸含量的影响

目的:探讨人参皂苷Rg1在运动性中枢疲劳中的作用机制,并研究其剂量效应关系. 方法:健康雄性昆明种小鼠,按体质量随机分为六组:即对照组和人参皂苷Rg1 5、10、20、40和80 ms/kg的5个剂量组,每组20只.每天分别用蒸馏水和5、10、20、40和80 ms/kg的人参皂苷Rg1灌胃.2周后,进行负重力竭游泳实验和无负重游泳90 min实验,测定力竭游泳时间以及无负重游泳后,脑组织中氨基酸含量的变化. 结果:与空白运动对照组相比,5、10和20 ms/kg人参皂苷Rgl组,脑组织中支链氨基酸(BCAA)含量和BCAA/AAA比值显著升高(P<0.01、P<0.01和P<0.05),而5、10、20和40 ms/kg人参皂苷Rg1组,脑组织中芳香族氨基酸(AAA)含量显著降低(P<0.01、P<0.05、P<0.05和P<0.05),其中色氨酸(Trp)含量,各组均显著降低(P<0.01);5和10 mg/kg组谷氨酸(Glu)含量显著降低(P<0.01和P<0.01),而天冬氨酸(Asp)、甘氨酸(Gly)各组间未见显著性差异.各组力竭游泳时间均有延长,其中5 ms/kg组(P<0.01)、10 ms/kg组(P<0.01)、20 ms/kg组(P<0.05),差异均有显著性意义. 结论:人参皂苷Rg1具有抑制中枢性疲劳,提高运动耐力的作用,以中低和低剂量效果最佳.     

Immunomodulatory activity of aqueous extract of Actinidia macrosperma

Actinidia macrosperma (AM) is a medicinal plant in China and has been well known for its activities against cancers, especially of lung, liver and digestive system. The immunomodulatory effects of AM aqueous extract were examined using S180-bearing mice. Young adult (20 +/- 2 g) ICR mice inoculated with S180 cells were divided randomly into six groups: S180-bearing control group, normal control group, positive control group (Ginseng Radix Rubra 2000 mg/kg) and 50, 100, 250 mg/kg AM treatment groups. Each group consisted of ten mice. Body and tumor weights were obtained after 12 consecutive days, and their humoral, cellular and nonspecific immune functions were also determined by relative assays. The results showed that the aqueous extract of AM was lack of significant inhibit on transplantable sarcoma S180, with a inhibit rate of 1.5% -14.8% (dose at 250 mg/kg was the best), but significantly increased the overall immune functions (especially at 100 and 250 mg/kg). The immunomodulatory effect was dose-dependent in a nonlinear fashion with the optimal dose of 100 mg/kg. The AM-induced antitumor effects were at least partially indirect and were associated with the modulation of immune functions.     

近交系小鼠微卫星位点的遗传标记多态性研究

目的研究近交系小鼠微卫星位点的遗传标记多态性及其意义。方法随机选取不同染色体的5个微卫星位点,应用PCR技术对BALB/C、豫医无毛小鼠、CBA、C57、DBA/2、C3H/HeJ、FVB/NJ及昆明小鼠共八种小鼠的DNA多态性进行研究。结果5对引物在近交系小鼠各基因座上均出现一条清晰的带,D16m it4、D3m it17、D3m it18具有多态性,D4m it2、D2Nds3不具有多态性。结论筛选出的引物D16m it4、D3m it17、D3m it18可用于检测近交系小鼠的品系来源和遗传背景,且所检测的小鼠符合近交要求。     

萘乙酸对睾丸细胞增殖与凋亡的影响

目的探讨萘乙酸(NAA)对小鼠睾丸细胞增殖与凋亡的影响。方法将25只健康雄性昆明种小鼠随机分为5组,每组5只。正常对照组和阳性对照组小鼠喂饲普通颗粒饲料,高、中、低剂量组的饲料中加入萘乙酸的剂量分别为5000、1000、200mg/kg,喂养4周处死小鼠,阳性对照组于处死前3天每天腹腔注射环磷酰胺20mg/kg。取睾丸组织,用MTT法测定各组睾丸细胞增殖活性,用免疫组织化学方法检测各组增殖细胞核抗原(PCNA)和半胱天冬酶(Caspase-3)表达。结果高剂量组和正常对照组的睾丸细胞增殖活性分别为(0.501±0.069)和(0.875±0.082),差别有显著性(P<0.05)。高剂量NAA组PCNA和Caspase-3阳性表达率分别为8.9%和41.3%,正常对照组分别为34.9%和9.1%,差异均有显著性(P<0.05)。结论萘乙酸有一定抑制睾丸细胞增殖活性、诱发睾丸细胞凋亡的作用。     

Antitumoral Potency by Immunomodulation of Chloroform Extract from Leaves of Nitraria retusa,Tunisian Medicinal Plant,via its Major Compounds β-sitosterol and Palmitic Acid in BALB/c Mice Bearing Induced Tumor

This study evaluated the antitumoral effect of Chloroform extract from Nitraria retusa leaves, via its major compounds β-sitosterols and palmitic acid. BALB/c mice were subcutaneously inoculated with B16-F10 cells, then treated intra-peritoneally after 7 days with the chloroform extract for 21 days. They were then euthanized, and the tumors were weighed. Lung parenchyma was analyzed. Lymphocyte and macrophages proliferation, cytotoxic T lymphocyte (CTL) activities were evaluated using the MTT assay. Macrophage phagocytosis was evaluated by measuring the lysosomal activity and nitric oxide production. Antioxidant activity was studied by cellular antioxidant activity on macrophage and splenocytes and by lipid peroxidation inhibitory activity in liver cells, kidney, and serum.

β-sitosterols and palmitic acid, major compounds of chloroform extract, impeded remarkably the expansion of the transplantable tumor, protected the lung parenchyma, and increased splenocytes proliferation and both CTL activities in tumor-bearing mice. β-sitosterols and palmitic acid were also seen to have enhanced lysosomal activity of host macrophages and antioxidant cellular activity. Also, they showed an inhibitory effect of lipid peroxidation.

Our results suggest that antitumoral effect of β-sitosterols and palmitic acid from chloroform extract is related with its immunomodulatory activity, and opens the way for a nutrition application and coprocessing phytotherapy against cancer.