EB病毒感染实验室诊断方法探讨

目的 探讨EB病毒(EBV)感染的实验室诊断方法.方法 设计针对EBV基因组的引物和荧光标记探针,利用实时荧光定量聚合酶链反应(FQ-PCR)检测70例外周血标本.其中17例是确诊EBV感染及传染性单核细胞增多症患儿,27例是临床高度怀疑EBV感染患儿,26例是临床非EBV感染的患儿,并与检测EBV抗体VCA-IgM的酶联免疫吸附试验(ELISA)进行比较.结果 确诊组、疑似组和对照组患儿FQ-PCR检测EBV的阳性率分别为100.00%、81.48%和0.00%.与此对应VCA-IgM的阳性率分别为100.00%、25.93%和0.00%.确诊组EBV DNA的平均拷贝数为1.15×105/ml,疑似组为4.38×104/ml,均明显高于对照组(P＜0.05).结论 与VCA-IgM检测方法相比,FQ-PCR检测到EBV感染的阳性率更高,EBV DNA的定量检测可帮助诊断儿科活动性EBV感染,尤其是可疑患儿的临床诊断.     

儿童原发性传染性单核细胞增多症中血清EBV抗体与DNA载量检测的诊断意义

目的 探讨EB病毒(EBV)抗体及血清EBV-DNA载量检测在诊断儿童EB病毒感染传染性单核细胞增多症中的意义.方法 收集2007年1月～2008年10月间入住北京儿童医院住院治疗的130例EBV感染传染性单核细胞增多症患儿,采用间接免疫荧光法检测EBV特异性抗体及抗体亲和力,荧光定量PCR方法检测血清中EBV-DNA载量.结果 在130例儿童EBV感染传染性单核细胞增多症病例中,入院时血清EBV抗体反应存在多种类型,抗EBV-CA-IgM阳性率达95.4%,随着时间推移,EBV特异性抗体及抗体亲和力出现相应的变化.77例进行血清EBV-DNA检测,总阳性率为15.6%(12/77),平均拷贝数为1.5×104copies/ml,动态监测提示EBV-DNA载量亦随时间变化而变化.结论 血清EBV-CA-IgM结合EBV-CA-IgG亲和力检测,可以提高原发性EBV感染传染性单核细胞增多症诊断的敏感度.血清中EBV-DNA检测在原发性EBV感染传染性单核细胞增多症诊断中的作用有待进一步研究.     

EB病毒感染实验室诊断方法探讨

目的探讨EB病毒(EBV)感染的实验室诊断方法。方法设计针对EBV基因组的引物和荧光标记探针,利用实时荧光定量聚合酶链反应(FQ-PCR)检测70例外周血标本。其中17例是确诊EBV感染及传染性单核细胞增多症患儿,27例是临床高度怀疑EBV感染患儿,26例是临床非EBV感染的患儿,并与检测EBV抗体VCA-IgM的酶联免疫吸附试验(ELISA)进行比较。结果确诊组、疑似组和对照组患儿FQ-PCR检测EBV的阳性率分别为100.00%、81.48%和0.00%。与此对应VCA-IgM的阳性率分别为100.00%、25.93%和0.00%。确诊组EBV DNA的平均拷贝数为1.15×105/m l,疑似组为4.38×104/m l,均明显高于对照组(P<0.05)。结论与VCA-IgM检测方法相比,FQ-PCR检测到EBV感染的阳性率更高,EBV DNA的定量检测可帮助诊断儿科活动性EBV感染,尤其是可疑患儿的临床诊断。     

EB病毒和调节性T细胞检测在诊断儿童急性B淋巴细胞白血病中的意义

目的探讨EB病毒(EBV)和调节性T细胞(Treg)检测在诊断儿童急性B淋巴细胞白血病(B-ALL)中的意义。方法2014年9月至2015年12月收治的45例B-ALL纳入B-ALL组,40例健康体检儿童纳入对照组,收集外周血标本,流式细胞仪测定Treg平均荧光强度(MFI),ELISA检测血浆抗EBV VCA IgG、抗EBV核抗原(EBNA)IgG(潜伏感染),抗EBV VCA IgM(急性感染)。分析B-ALL组EBV感染情况和Treg比例变化及意义。结果 B-ALL组Treg比例与FoxP3 MFI明显高于对照组,差异均有统计学意义(P=0.004,P=0.001)。B-ALL组中EBV急性感染、潜伏感染和血清学检查阴性者的年龄、性别、血红蛋白、血小板计数、白细胞计数和骨髓原始细胞比例比较,差异均无统计学意义(P0.05)。B-ALL组潜伏感染者Treg比例和FoxP3MFI均高于其他被试,差异有统计学意义(P0.05);对照组血清反应阴性者Treg比例和FoxP3MFI均低于其他被试,差异均有统计学意义(P0.05);其他类型被试Treg比例和FoxP3 MFI比较,差异均无统计学意义(P0.05)。Common-B-ALL者较pro-B-ALL和pre-B-ALL者Treg比例减少,差异有统计学意义(P=0.020),而FoxP3 MFI和EBV感染情况比较,差异无统计学意义(P0.05)。EBV潜伏感染评估对B-ALL发病风险的OR值为4.060,95%CI为1.21~13.64(P=0.020),即EBV潜伏感染是B-ALL发病的危险因素。结论 EBV潜伏感染和Treg比例升高在儿童B-ALL的诊断中具有重要意义,潜伏感染是儿童B-ALL发病的危险因素。     

血液系统恶性肿瘤患者巨细胞病毒和EB病毒定量检测的临床价值

目的评价实时荧光定量聚合酶链反应对血液系统恶性肿瘤患者巨细胞病毒(CMV)感染和EB病毒(EBV)感染的早期诊断价值。方法应用实时荧光定量PCR检测334例血液系统恶性肿瘤患者血液中CMV DNA和EBV DNA载量。结果 334例患者中EBV检测阳性率为13.2%,CMV检测阳性率为19.2%,DNA拷贝数介于(102~107)copy/mL之间。治疗有效者EBV DNA和CMV DNA载量迅速下降,2~3周后转阴。结论实时荧光定量PCR动态监测血液系统恶性肿瘤患者CMVDNA和EBV DNA水平对于CMV感染和EBV感染的早期诊断和疗效判断具有重要意义。     

霍奇金淋巴瘤初次治疗患者EBV感染的预后研究

目的:探讨EB病毒(EBV)感染对初次治疗霍奇金淋巴瘤(HL)患者生存的影响,为临床研究提供参考依据。方法:共纳入120例诊断为霍奇金淋巴瘤的患者作为研究对象,采用检测EBV编码的RNA(EBER)或蜡块中潜伏膜蛋白-1(LMP-1)确诊EBV感染情况,将患者分为EBV+和EBV-组,分析EBV感染与临床特征的相关性,并随访观察其对患者生存的影响,将患者分为死亡组和存活组,对影响生存状况的因素进行分析。结果:120例HL患者中有36例(30.0%)存在EBV感染。EBV~+组中男性、6-15和61-74岁、混合细胞型比例明显高于EBV-组,而结节硬化型明显低于EBV-组(P0.05)。EBV+组1和2年总生存率分别为88.9%和83.3%,明显低于EBV-组97.6%和95.2%(P0.05)。120例HL患者中,死亡10例(8.3%)。死亡组61-74岁、未接受过放疗、EBV~+比例明显高于存活组(P0.05)。多因素分析发现:61-74岁及EBV+是影响生存状况的危险因素(P0.05)。结论:EBV感染与HL有一定相关性,EBV~+与EBV~-组患者的临床特征存在差异,且EBV~+组总生存时间较EBV~-组短,EBV~+是影响HL患者总生存时间的危险因素。     

深圳市罗湖区儿童EB病毒感染情况及流行病学分析

目的了解深圳市罗湖区儿童EB病毒(EBV)感染情况。方法回顾性分析深圳市罗湖医院集团2018年1616例疑似EBV感染患儿EBV检测结果。用荧光定量PCR方法检测全血或咽拭子EBV DNA载量,用酶联免疫法检测EBV血清学抗体,对不同性别、年龄、季节的患儿EBV感染情况进行统计分析。结果1616例疑似EBV感染患儿总体感染率为25.6%,其中男性感染率为23.4%,女性为28.7%。不同年龄组间EBV感染率差异有统计学意义(P<0.01),其中学龄组(6~12岁)阳性率最高(40.2%)。秋季感染率最高(32.0%),四季感染率差异有统计学意义(χ2=20.693,P<0.001)。支气管肺炎是EBV感染引起的最常见疾病,发生率高达40.2%。结论深圳市罗湖区儿童EBV感染率偏低,秋季感染率最高,且随年龄增长感染有上升趋势,感染无性别差异。EBV感染相关疾病主要以支气管肺炎为主,无相关重症疾病感染。     

荧光定量PCR检测儿童非典型EB病毒感染的临床评价

目的探讨荧光定量PCR检测对诊断和治疗非典型EB病毒(EBV)感染的临床意义。方法对2007-04-2009-10诊断为非典型EBV感染90例患儿,抽静脉血2 ml,注入枸橼酸钠抗凝剂与静脉血充分混匀,用核酸扩增荧光PCR法测定基因拷贝数,同时进行常规外周血及异型淋巴细胞检查,对照病因和首发临床表现,分析儿童感染非典型EBV时荧光定量PCR检测的基因拷贝数变化。结果 EBV-DNA拷贝数能早期反映出非典型EBV感染,拷贝数越高,出现多器官损害概率越高。结论荧光定量PCR检测EBV-DNA拷贝数有助于早期诊断非典型EBV感染,能减少诊疗的盲目性,对临床有一定指导意义。     

肾移植术后受者EB病毒感染回顾性分析

目的 探讨肾移植受者术后EB病毒(EBV)的感染情况和对预后的影响. 方法 回顾性分析2012年11月至2016年4月进行肾移植手术受者共127例资料,根据术后外周血EBV DNA(实时荧光定量PCR方法)的结果,分析受者EBV感染的特点. 结果 127例肾移植病例中,EBV DNA阳性23例,阳性率18.11％;定量对数平均值(3.48±0.42) IU/mL;EBV最早检出时间为术后第2周,最晚检出时间为术后第11周,其中第5周检出率最高;使用更昔洛韦抗病毒治疗,73.91％阳性受者在1～3周转阴.EBV阳性组与阴性组术后3个月血清尿素、肌酐平均水平分别为(11.2±2.1) mmol/L、(152.8±15.8)μmol/L和(8.4±1.7)mmol/L、(108.6±35.7)μmol/L,两组间差异无统计学意义(P＞0.05).结论 EBV感染是肾移植术后的并发症并且感染发生率较高,临床医生应该引起足够的重视,积极采取有效预防措施和治疗方案,将有助于改善患者的预后.     

原发与再激活EB病毒感染相关噬血细胞性淋巴组织细胞增生症的比较分析

目的:比较原发性EB病毒(EBV)感染及EBV再激活相关噬血细胞性淋巴组织细胞增生症(HLH)患儿的临床特征，探讨不同EBV感染状态对HLH临床指标及预后的影响。方法:收集河南省儿童医院2016年6月至2021年6月收治的51例EBV相关HLH患儿的临床资料，根据血浆EBV抗体谱检测结果，将患者分为EBV原发性感染HLH组(18例)和EBV再激活HLH组(33例)。分析比较两组的临床特征、实验室指标及预后。结果:两组患者在年龄、性别、肝肿大、脾肿大、淋巴结肿大、外周血中性粒细胞数、血红蛋白含量、血小板数、血浆EBV-DNA载量、乳酸脱氢酶、谷丙转氨酶、谷草转氨酶、白蛋白、纤维蛋白原、甘油三酯、铁蛋白、骨髓噬血现象、NK细胞活性、sCD25等方面均无显著差异(P> 0.05),EBV再激活HLH组中枢神经系统受累及CD4/CD8明显高于原发EBV感染HLH组(P <0.05)，而总胆红素明显低于原发EBV感染HLH组(P <0.05);参照HLH-2004方案治疗后，EBV再激活HLH组患者的缓解率、5年OS率、5年EFS率均明显低于EBV原发感染HLH组(P <...     

Analysis of Epstein-Barr virus in hodgkin's disease: Experience of a single university hospital in Korea

Hodgkin's disease is known to be associated with Epstein-Barr virus (EBV) infection in Western countries, and viral nucleic acids and proteins have been identified within Reed-Sternberg (RS) cells, which are the histopathologic hallmark of the disease process. Twenty-five cases of Hodgkin's disease from a single university hospital in Korea were studied for evidence of EBV by in situ hybridization for EBV DNA and RNA and immunohistochemistry for an EBV latent protein. EBV nucleic acids were studied by a rapid (60 minutes) in situ hybridization procedure, which utilized biotinylated DNA probes specific for the following nucleic acid sequences: (1) EBV EBER1 RNA (an abundant RNA sequence expressed during latent EBV infection), (2) EBV Notl repeats (a tandemly repeated DNA sequence, which has been established to identify amplified EBV genome in lytic EBV infection), and (3) BAM HI W (a DNA sequence reiterated 11 times within the viral genome). In addition, immunohistochemistry for EBV latent membrane protein, a protein that is capable of inducing cellular transformation in cell culture, was also performed. EBV was identified within the neoplastic RS cells by at least one method in 19/25 cases (76%). The mixed cellularity subtype was the most common subtype associated with EBV infection (11/13–85%). In situ hybridization for EBV EBER1 RNA was the most sensitive method for EBV detection and was present in 17/25 cases. A significant proportion of Korean Hodgkin's disease cases is associated with EBV infection. © 1994 Wiley-Liss, Inc.     

EB病毒感染机制及临床研究进展

EB病毒（EBV）属于疱疹病毒γ亚科,是双链DNA病毒,人群普遍感染。其可逃避机体的免疫攻击,在B淋巴细胞内建立持续终身的潜伏感染。EBV感染可引起机体不同程度的免疫反应,可累及机体几乎所有脏器和组织,引起多种非恶性淋巴增殖性疾病、肿瘤性疾病及自身免疫病等。EBV的实验室检查有助于EBV感染的诊断及感染时相的判别。EBV感染机制复杂,感染后临床疾病谱广,轻重不一,预后千差万别,了解EBV感染机制及其相关疾病的预后情况及预后相关因素,对临床上进行更好的预防及治疗有重大意义。本文就EBV感染相关疾病、临床检测、治疗及预后的研究进展作一综述。     

Real-time Epstein-Barr virus PCR for the diagnosis of primary EBV infections and EBV reactivation.

BACKGROUND: The serological diagnosis of primary Epstein-Barr virus (EBV) infections is often difficult, whereas the relevance of elevated immunoglobulin G (IgG) antibodies against early antigen (EA) for the diagnosis of EBV reactivation has increasingly become a matter of dispute. Recently, EBV PCR has been added as a diagnostic tool. Positive EBV PCR has been demonstrated in the serum of patients with primary EBV infections and EBV reactivation. OBJECTIVES: To compare classical serological diagnosis of primary EBV infection and EBV reactivation with real-time EBV PCR. STUDY DESIGN: Sera from 45 patients were selected with detectable immunoglobulin M (IgM) antibodies against EBV viral capsid antigen (VCA), and 62 sera were selected with a reactivation profile. A real-time EBV PCR was performed with DNA extracted from these sera. RESULTS: Based on serological data, the diagnosis of primary EBV infection was established for 24 of the 45 IgM VCA-positive patients. By performing PCR, seven extra cases of primary infection were diagnosed for which no heterophilic antibodies could be detected. In five cases of primary infection, no EBV DNA could be detected by PCR. Only in two of the 62 sera with a reactivation seroprofile could EBV DNA be detected. CONCLUSIONS: Based on these data, we suggest that for the diagnosis of primary infections, EBV PCR could lead to an increase of >16% in the number of positive diagnoses by confirming a positive IgM VCA in the absence of heterophilic antibodies. Furthermore, EBV PCR is positive in only 3% of sera with elevated antibodies against EA, raising doubt as to the utility of EA titers for diagnosing EBV reactivation.     

儿童EB病毒感染临床相关因素分析

目的 探讨儿童EB病毒(EBV)感染的临床特点,提高对儿童EB病毒感染的认识.方法 对经酶联免疫染色法(ELISA)检测EBV抗体确定为EB病毒感染的50患儿进行回顾性分析.结果 50例EB病毒感染患儿中,男32例,女18例,男女之比1.8:1,以呼吸道感染为主29例(58.0%),其中上呼吸道感染12例(24.0%)、扁桃体炎8例(16.0%)、支气管炎7例(14.0%)、肺炎2例(4.0%),传染性单核细胞增多症11例(22.0%).结论 小儿EB病毒感染可累及全身多系统,不典型表现者居多,易误诊漏诊,需提高对本病的认识,综合分析,早期诊断,早期治疗.     

Clinical reliability of IgG, IgA, and IgM antibodies in detecting Epstein-Barr virus at different stages of infection with a commercial nonrecombinant polyantigenic ELISA

We studied the diagnostic reliability of a modification of the Enzygnost EBV test (Behringwerke, Germany) for the detection of IgG, IgA, and IgM antibodies (Abs) in the diagnosis of Epstein-Barr virus (EBV) disease. One hundred and twenty-three serum samples were studied: 14 asymptomatic subjects without EBV infection, 48 patients with primary infection, 46 subjects with past EBV infection (11 patients with other acute infections), 8 patients without EBV infection but with other viral infection, and 7 patients with probable acute clonal stimulation of B lymphocytes caused by different microorganisms. Enzygnost EBV is based on an ELISA test with a pool of viral antigens. In our series the reliability of IgM for the diagnosis of recent primary EBV infection was: sensitivity 100%, specificity 95%, positive predictive value 90.5%, and negative predictive value 100%. The IgG detection with Enzygnost was: sensitivity 98%, specificity 100%, positive predictive value 100%, and negative predictive value 91.7%. Only two subjects had positive IgA. The Enzygnost test is an efficient method for the diagnosis of EBV infection although a few IgM false positives can occur.     

X-linked lymphoproliferative syndrome. Natural history of the immunodeficiency.

The X-linked lymphoproliferative syndrome is characterized by immunodeficiency to Epstein-Barr virus (EBV) manifested by severe or fatal infectious mononucleosis and acquired immunodeficiency. We studied immune responses in six males of a well-characterized kindred with the X-linked lymphoproliferative syndrome. Two males were studied before and during acute fatal EBV infection. Both individuals demonstrated normal cellular and humoral immunity before EBV infection. During acute EBV infection, both individuals developed vigorous cytotoxic cellular responses against EBV-infected and -uninfected target cells. Anomalous killer and natural killer T cell activity was demonstrated against a variety of lymphoid cell lines, autologous fibroblasts and autologous hepatocytes. Effector cells responsible for anomalous killing reacted with a pan-T cell monoclonal antibody, and belonged to the OKT.8 T cell subset. Death in each case was caused by liver failure, but one patient developed extensive liver necrosis, whereas the other developed a massive infiltration of the liver with EBV-infected immunoblasts after aggressive immunosuppressive therapy. Immunological studies were performed on four males who had survived EBV infection years previously. They demonstrated global cellular immune defects with deficiencies of lymphocyte proliferative responses to mitogens and antigens, humoral immune deficiencies, abnormalities of regulatory T cell subsets and deficient natural killer cell activity. We propose that an aberrant immune response triggered by acute EBV infection results in unregulated anomalous killer and natural killer cell activity against EBV infected and uninfected cells. These studies suggest that global immune defects appearing in males with X-linked lymphoproliferative syndrome who survive EBV infection are epiphenomenon.     

Transfusion‐related Epstein‐Barr virus infection among stem cell transplant recipients: a retrospective cohort study in children

BACKGROUND: Blood safety warrants strict screening measures to minimize the risk of transmitting blood‐borne pathogens. However, transfusion‐transmitted infections for which testing is not currently performed continue to be a concern. Among these untested agents is Epstein‐Barr virus (EBV) which, in the transplant setting, is associated with lymphoproliferative disease, a potentially fatal cancer. The aim of this study was to analyze the incidence of posttransplant EBV infection and its association with administration of blood products in children receiving a hematopoietic stem cell (HSC) graft. STUDY DESIGN AND METHODS: This retrospective cohort study sought to review charts of pediatric recipients of HSC grafts to collect information on the presence of EBV antibodies in the recipients' pretransplant sera and in HSC donor sera, incidence of posttransplant EBV infection, and patients' transfusion history. Cumulative incidence of posttransplant EBV infection was estimated by Kaplan‐Meier methods according to pretransplant serology. The association between blood products and EBV infection was measured by Cox regression models. RESULTS: The pretransplant EBV seroprevalence was 77.9% for recipients and 61.8% for graft donors. Virtually, all recipients received blood products during the peritransplant period. Among seronegative recipients, the 30‐ and 60‐day cumulative incidences of posttransplant EBV infection were 4.6 (95% confidence interval [CI], 1.2‐17.3) and 13.4% (95% CI, 5.8%‐29.4%), respectively. The 60‐day cumulative incidence was 8.3% (95% CI, 2.2%‐29.4%) when restricting the analysis to seronegative recipients of cord blood grafts. Importantly, there was a clear positive trend associating EBV infection to transfusion volume. CONCLUSION: This study suggests an association between transfusions and posttransplant EBV infection in HSC transplant recipients.     

CD3-negative lymphoproliferative disease of granular lymphocytes containing Epstein-Barr viral DNA.

Lymphoproliferative disease of granular lymphocytes (LDGL) is a heterogeneous disorder and the pathogenesis is likely to be complex. Some patients with chronic active EBV (CAEBV) infection also have LDGL. To investigate the relationship between EBV infection and the pathogenesis of LDGL, we conducted a survey for EBV DNA sequences by Southern blot analysis of DNA obtained from the peripheral blood of seven patients with LDGL, including one with CAEBV infection. Interestingly, EBV DNA was detected in the sample from the patient with CAEBV infection, and in the samples from four other patients with CD3-LDGL. Moreover, a single band for the joined termini of the EBV genome was demonstrated in two samples, suggesting a clonal disorder of those LDGL. These findings strongly suggest that EBV may play a pathogenic role in some cases of LDGL.     

EB病毒IgM、IgG及DNA检测在儿童呼吸道感染性疾病中的诊断价值分析

目的分析EB病毒(EBV)IgM、IgG及EBV-DNA在儿童呼吸道感染性疾病中的诊断价值。方法选取400例呼吸道感染患儿作为观察组,100例非呼吸道感染住院患儿作为对照组,采用酶联免疫吸附法(ELISA)检测患儿的EBV-IgM和IgG,荧光定量PCR(FQ-PCR)检测病毒DNA。比较观察组和对照组患儿的EBV-IgM、IgG及EBV-DNA阳性率,评价不同指标的诊断价值。结果观察组患儿EBV-IgM阳性率51.00%,EBV-IgG阳性率58.50%,EBV-DNA阳性率56.25%;对照组患儿EBV-IgM阳性率4.00%,EBV-IgG阳性率11.00%,EBV-DNA阳性率5.00%,差异具有统计学意义(P0.05)。EBV-IgM灵敏度51.00%,特异度96.00%,曲线下面积0.617;EBV-IgG灵敏度58.50%,特异度89.00%,曲线下面积0.583;EBV-DNA灵敏度56.25%,特异度95.00%,曲线下面积0.642。结论 EBV-IgM、IgG及EBV-DNA在儿童呼吸道感染中具有诊断价值。     

荧光定量聚合酶链反应法检测咽拭子EBV—DNA在早期诊断EBV感染中的作用

目的 探讨咽拭子荧光定量聚合酶链反应（FQ-PCR）法检测非洲淋巴细胞瘤病毒脱氧核糖核（EBV—DNA）在早期诊断EBV感染中的应用价值。方法对于疑诊EBV感染的患儿在疾病初期分别采用咽拭子PCR法检测EBV—DNA,同时用酶联免疫吸附测定（ELISA）法检测静脉血EBV—VCA—IgM和（或）IgG,对于前者阳性而后者阴性的病例在1周后复查EBV—VCAIgM和IgG,比较两者在早期诊断EBV感染中的价值。同期检测健康儿童50例作为对照组。结果①共检测疑诊病例985例,其中EBV—DNA阳性344例,阳性率34．9％,EBV—VCA—IgM和（或）IgG阳性164例,阳性率16．6％;健康对照组检测50例,仅1例EBVDNA阳性,所有病例EBV—VCA—IgM和（或）IgG均阴性。②在纳入研究病例中,诊断为传染性单核细胞增多症者38例,其中EBV—DNA37例阳性、EBV—VCAIgM和（或）IgG27例阳性（P〈0．05）。诊断为非典型EBV感染的312例,其中EBVDNA阳性307例,阳性率98．4％,EBV—VCA—IgM和（或）IgG阳性137例,阳性率43．9％（P〈0．01）。结论咽拭子PCR法检测EBV—DNA敏感度高,特异度强,且取材简单,方法无创,家长易于接受,与检测EBV-VCA—IgM、IgG相比,在早期诊断EBV感染性疾病,尤其是非典型EBV感染很有应用价值。