溃疡性结肠炎患者血清PGE2、PAF测定的临床价值

【目的】探讨溃疡性结肠炎（UC）患者血清前列腺素E2（PGE2）、血小板活化因子（PAF）的变化以及与疾病活动性的相关性。【方法】采用酶联免疫吸附试验检测32例活动期和缓解期UC患者血清PGE2、PAF含量变化,并同时进行临床活动指数（CAI）评分的比较。以30例健康体检者作为对照组。【结果】活动期UC患者血清PGE2、PAF含量均明显高于缓解期组和正常对照组（均P〈0．01）,缓解期组PGE2、PAF含量与正常对照组比较无明显差异（均P〉0．05）。活动期组{临床活动指数cAI评分为（7．16±3．32）,明显高于缓解期组CAI评分（2．09±1．46）（P〈0．01）。活动期PGE。、PAF含量与活动期CAI评分均呈正相关（r=0．619,P〈0．01;r=0．561,P〈0．01）。缓解期PGE2、PAF含量与缓解期CAI评分均呈正相关（r=0．418,P〈0．05;r=0．437,P〈0．05）。【结论】活动期UC患者血清PGE2、PAF水平明显升高,可作为判定UC患者疾病活动的指标,有利于疾病的监测和随访。     

检测CA199、CA125、CA153及CEA在肿瘤诊断中的意义

目的探讨血清癌抗原199（CA199）、癌抗原125（CA125）、癌抗原153（CA153）和癌胚抗原（CEA）检测在对不同类型恶性肿瘤的诊断价值。方法采用全自动化学发光免疫分析法,检测62例不同类型肿瘤患者、14例非肺癌患者组及30例正常对照组血清CA199、CA125、CA153及CEA水平。结果胰腺癌患者组CA199、CEA水平显著高于对照组（P〈0．01、P〈0．05）,CA199的阳性率达100％;胃癌患者组CA199、CA125、CA153、CEA水平显著高于对照组（P〈0．01或〈0．05）：乳腺癌组CA153、CA125、CA199水平均明显高于对照组（P〈0．01或〈0．05）;卵巢癌患者组CA125,CA153水平显著高于对照组（P〈0．01或P〈0．05）;肺癌患者组CA199,CA125及CEA水平明显高于非肺癌对照组及正常对照组（P均〈0．01）。CA199、CA125及CEA联检特异性92．9％、敏感性79．3％。结论CA199测定对胰腺癌的诊断提供可靠依据。CA199、CA125及CEA联合检测对肺癌的诊断有重要意义。CA199、CA125、CA153及CEA4项指标联检对胃癌和乳腺癌的诊断有一定价值。CA125、CA153对卵巢癌的诊断有一定价值。     

血清CA125和CA199检测对卵巢癌诊断应用价值的探讨

目的：通过测定血清中肿瘤标志物 CA125和 CA199水平,探讨其对卵巢癌诊断的应用价值。方法2011年1月至2012年10月确诊的卵巢癌患者61例,卵巢良性疾病患者68例,健康对照者50例,测定血清中 CA125和CA199水平。结果卵巢癌组血清 CA125和 CA199水平均明显高于卵巢良性疾病组和健康对照组,差异有统计学意义（P ＜0．05）;卵巢良性疾病组血清 CA125和 CA199水平与对照组比较,差异无统计学意义（P ＞0．05）;CA125和CA199联合检测的敏感性明显高于 CA125和 CA199单独检测（P ＜0．05）。结论CA125和 CA199水平进行测定及其联合检测对卵巢癌的诊断、分期和治疗有重要意义。     

基质金属蛋白酶-1及其组织抑制因子-1、肿瘤坏死因子-α在溃疡性结肠炎患者外周血中的表达及其意义

目的探讨基质金属蛋白酶-1（MMP-1）及其组织抑制因子-1（TIMP-1）、肿瘤坏死因子-α（TNF-α）在溃疡性结肠炎患者外周血中的表达及其意义。方法46例溃疡性结肠炎患者与40例健康体检者,分别设为UC组与对照组。清晨空腹采取静脉血,离心分离血浆,ELISA法检测血浆中MMP-1、TIMP-1、TNF-α水平。结果UC组患者（活动期,缓解期）血浆MMP-1、TIMP-1、TNF-α水平明显高于对照组（P〈0．05）;UC组活动期MMP-1、TIMP-1、TNF-α水平明显高于缓解期（P〈0．05）。不同病情UC患者血浆MMP-1、TIMP-1、TNF-α水平有显著差异（P〈0．05）,病情越重,血浆MMP-1、TIMP-1、TNF-α水平越高。结论外周血MMP-1、TIMP-1、TNF-α水平可反映UC病情变化。     

溃疡性结肠炎患者血清PGE2、PAF水平变化及其临床意义

目的探讨血清前列腺素E2（PGE2）、血小板活化因子（PAF）在溃疡性结肠炎（UC）患者血清中的表达水平变化及临床意义。方法采用酶联免疫吸附试验检测59例活动期和21例缓解期UC患者血清中PGE2、PAF含量变化,并同时进行临床活动指数（CAI）评分的比较。以40例健康体检者作为对照组。结果活动期UC患者血清PGE2、PAF含量均明显高于缓解期组和正常对照组（均P〈0.01）,缓解期组PGE2、PAF含量与正常对照组比较无明显差异（均P〉0.05）。活动期组临床活动指数CAI评分明显高于缓解期组CAI评分（P〈0.01）。活动期组患者血PGE2、PAF含量与活动期CAI评分均呈正相关（P〈0.01）;缓解期组血PGE2、PAF含量与缓解期CAI评分均呈正相关（P〈0.05）。结论活动期UC患者血清PGE、PAF水平明显升高,可作为判定UC患者疾病活动的指标,对于判断溃疡性结肠炎病情变化,有一定的临床价值。     

溃疡性结肠炎患者血清抗β2糖蛋白Ⅰ抗体水平的检测及其意义

目的探讨溃疡性结肠炎患者血清中抗β2糖蛋白Ⅰ抗体（aβ2GPⅠ）水平及其意义。方法采用ELISA方法分别对活动期溃疡性结肠炎组、缓解期溃疡性结肠炎组及正常对照组血清中的aβ2GPⅠ水平和阳性率进行检测分析。结果①活动期及缓解期溃疡性结肠炎患者血清中IgM和IgG型aβ2GPⅠ水平明显高于正常对照组；活动期溃疡性结肠炎组与缓解期组相比，IgG型aβ2GPⅠ水平差异亦有统计学意义（P〈0．05）。②在aβ2GPⅠ阳性率的比较中，活动期溃疡性结肠炎组与缓解期溃疡性结肠炎组比较差异有统计学意义；二者与正常对照组相比，差异均有统计学意义。而活动期溃疡性结肠炎组与正常对照组相比差异有统计学意义（P〈0．01）。结论抗β2糖蛋白Ⅰ抗体可能和溃疡性结肠炎的发病机制有关，是其高凝状态的可能原因之一。     

溃疡性结肠炎患者血清TNF-α与内毒素水平检测及临床意义

目的探讨溃疡性结肠炎（uc）患者血清肿瘤坏死因子-α（TNF—α）及内毒素（ET）水平变化及临床意义。方法对UC患者组患者（轻度15例,中度22例,重度15例）及正常对照组的40例健康体检者血清11NF-α、ET水平进行检测,并进行比较分析。结果UC组患者血清TNF—α、ET平均水平均明显高于正常对照组,差异均具有统计学意义（P〈0．05）;重度UC患者血清TNF—α、ET水平显著高于轻、中度患者,差异亦有统计学意义（P〈0．05）;各不同病情UC患者血清TNF-α、ET水平均呈显著正相关（P〈0．05）。结论测定血清TNF—α、ET水平对溃疡性结肠炎患者的病情观察及疾病诊疗具有重要的,临床意义。     

溃疡性结肠炎患者血清细胞因子检测结果分析

目的：探讨溃疡性结肠炎（UC）患者血清细胞因子与病情严重性的关系。方法选取87例 UC 患者（疾病组）与26例健康人（对照组）作为研究对象。采用溃疡性结肠炎活动指数（UCAI）评估 UC 患者的严重程度。采用酶联免疫法（ELISA）定量测定 UC 患者血清白细胞介素-1β（IL-1β）、白细胞介素-4（IL-4）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）、干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α）的浓度,并比较 UC 患者与健康人以及不同严重程度 UC 的细胞因子水平。结果UC 患者血清 IL-1β、IL-8和 TNF-α水平显著高于对照组（P ＜0．05）,UC 患者血清中 IL-10水平显著低于对照组（P ＜0．05）,两组间的 IL-4与IFN-γ水平的差异无统计学意义（P ＞0．05）。根据 UCAI 标准评分,重度活动期、中度活动期、轻度或非活动期的 UC 患者数量分别为17例、29例和41例。重度活动期 UC 患者血清 IL-8、TNF-α水平显著高于中度、轻度或非活动期 UC 患者（P ＜0．05）;IL-1β、IL4、IL-10与 IFN-γ水平在不同活动期的 UC 患者中的差异无统计学意义（P ＞0．05）。结论细胞因子 IL-8、TNF-α与 UC患者病情相关,可能对 UC 的病情评估有帮助。     

溃疡性结肠炎患者CA125及内毒素检测价值的探讨

目的探讨检测癌抗原125(CA125)及内毒素对溃疡性结肠炎(UC)患者的临床意义。方法回顾性分析2009年6月至2010年12月80例UC患者的CA125及内毒素检测资料,并与同期80例健康体检者的CA125及内毒素检测资料相对照分析。结果 UC活动期患者的CA125水平明显高于UC缓解期患者和健康受试者(P<0.05),而UC缓解期患者的CA125水平与健康受试者之间无显著差异(P>0.05)。将UC活动期患者分为轻度、中度、重度,三组患者的CA125水平并无统计学差异(P>0.05)。UC活动期患者的内毒素水平明显高于UC缓解期患者和健康受试者(P<0.05),而UC缓解期患者的内毒素水平与健康受试者之间无差异(P>0.05)。将UC活动期患者分为轻度、中度、重度,内毒素水平随着病情加重逐渐上升,三组患者的内毒素水平有统计学差异(P<0.05)。结论活动期UC患者的CA125和内毒素水平高于缓解期患者和健康受试者,其内毒素水平与UC的病情严重程度相关。     

CA125和HE4联合检测在早期卵巢癌诊断中的应用价值

目的：研究血清糖类抗原125（CA125）和人附睾蛋白4（HE4）联合检测对早期卵巢癌诊断的应用价值。方法选取本院2014年1月至2015年12月88例卵巢癌患者作为观察组（卵巢组）,70例良性卵巢囊肿患者作为良性肿瘤对照组,同期健康体检70例作为健康对照组,采用电化学发光法检测血清CA125、HE4,比较三组患者的血清 HE4和CA125水平差异。结果卵巢癌组血清 HE4、CA125显著高于良性肿瘤组及健康对照组,有统计学差异（P＜0．05）。HE4、CA125联合检测灵敏度显著高于单独检测 HE4、CA125,具有统计学差异,（P＜0．05）。联合检测特异性与单独检测无统计学差异（P＞0．05）。结论血清CA125和HE4联合检测可显著提高早期卵巢癌诊断的敏感性及准确性。     

Cancer antigen 125 levels in inflammatory bowel diseases

Background: Cancer antigen 125 (CA‐125) is a tumor marker used for the diagnosis and monitoring of ovarian carcinoma. It can also be elevated in endometriosis, inflammations, and in nongynecological malignancies. Up to date, serum CA‐125 levels in inflammatory bowel diseases (IBD) have not been studied before. Aim: To assess the levels of CA‐125 in patients with ulcerative colitis (UC) and Crohn's disease (CD). Methods: Serum levels of CA‐125 were investigated in 68 cases with UC (male/female: 47/21), 32 CD (male/female: 21/11), and 31 healthy controls (male/female: 16/15). Levels of CA‐125 were also compared among UC patients according to lesion location, severity, and activity of CD. Results: Serum CA‐125 levels were 17.29±24.50 U/ml, 15.56±20.74 U/ml, and 8.85±2.62 U/ml in patients with UC, CD, and healthy controls, respectively. Serum CA‐125 levels were significantly higher in UC compared to control group (P=0.001). Serum CA‐125 levels were higher in CD patients compared to control group but there was no significance (P=0.087). Serum CA‐125 levels were higher in pancolitis compared to distal type and left‐sided UC. Conclusions: Our data suggest that serum CA‐125 levels may be increased in patients with IBDs. J. Clin. Lab. Anal. 23:244–248, 2009. © 2009 Wiley‐Liss, Inc.     

C反应蛋白在儿童溃疡性结肠炎患者临床疗效和预后评估中的作用

目的对儿童溃疡性结肠炎(UC)患者进行检测血清中C反应蛋白(CRP)水平,并分析CRP与UC患儿的临床疗效和预后之间的关系。方法收集25例活动期UC患儿、14例缓解期UC患儿及32例健康儿童血清标本,检测CRP水平,研究CRP与UC疾病活动程度的关系。对UC患儿进行有效治疗,进一步观察CRP与临床治疗效果和预后的相关性。结果活动期UC患儿血清CRP水平比缓解期患儿及正常儿童明显升高,差异有统计学意义(P0.001)。活动性UC患儿经有效治疗后,病情缓解,血清中CRP水平显著下降(P0.001),接近缓解期UC患儿和健康者。结论 CRP在活动性UC患儿血清中显著升高,并能判断疾病的预后转归。     

HIF-1α、COX-2、CRP及ESR在溃疡性结肠炎患者外周血及黏膜组织中的表达及临床意义

目的研究溃疡性结肠炎(UC)患者外周血和黏膜组织中缺氧诱导因子1α(HIF-1α)、环氧酶-2(COX-2)、C-反应蛋白(CRP)和红细胞沉降率(ESR)表达水平,并探讨其临床意义。方法回顾性选取2018年1月至2020年12月在北京中医医院顺义医院治疗的UC患者120例作为研究对象,根据Walmsley评分分为缓解期40例(Walmsley评分<4分)和活动期80例(Walmsley评分>4分)。并选择同期结肠镜检测健康患者15例作为对照组。比较3组患者外周血HIF-1α、COX-2、CRP及ESR水平,以及黏膜组织HIF-1α、COX-2、CRP的表达水平。结果与对照组相比,UC患者血清HIF-1α、COX-2、CRP含量以及外周血ESP显著升高,差异均有统计学意义(P<0.05);活动期UC患者血清HIF-1α、COX-2、CRP含量以及外周血ESP显著高于缓解期UC患者,差异均有统计学意义(P<0.05)。与对照组相比,UC患者黏膜组织HIF-1α、COX-2和CRP mRNA和蛋白表达水平显著升高,差异均有统计学意义(P<0.05);活动期UC患者黏膜组织HIF-1α、COX-2和CRP mRNA和蛋白表达水平显著高于缓解期UC患者,差异均有统计学意义(P<0.05)。结论HIF-1α、COX-2、CRP及ESR不仅与UC的发病有关,而且与UC疾病进展有关。     

溃疡性结肠炎患者外周血单个核细胞中TREM-lmRNA 的表达水平

目的 通过比较髓系细胞触发受体-1(TREM-1)mRNA在活动期和缓解期溃疡性结肠炎(UC)患者以及健康体检者外周血单个核细胞(PBMC)中的表达水平,探讨TREM-1与UC疾病活动性的关系.方法 依据结肠镜检和病理活检结果,将UC患者分为活动期和缓解期2组,采用实时荧光定量逆转录(FQ-RT)-PCR检测70例UC患者(活动期患者38例,缓解期患者32例)和20名健康体检者PBMC中TREM-1 mRNA的表达水平,采用相对定量ACt值比较基因表达水平的高低,并比较TREM-1 mRNA表达水平与ESR和C反应蛋白(CRP)的相关性.结果 UC活动期患者PBMC中TREM-1 mRNA的表达水平(4.19±1.86)显著高于uC缓解期患者(5.29±1.71,P=0.007)和健康体检者(5.19±1.04,P=0.032),而UC缓解期患者与健康对照组差异无统计学意义(P=0.892);TREM-1 mRNA表达的受试者操作特性曲线(ROC)下面积为0.763(P<0.001),表明TREM-1 mRNA表达水平对UC疾病活动性具有诊断价值;当△Ct值为4.63时,是判断UC活动性的最佳临界点,其诊断的敏感度和特异度分别为73.7%和68.7%;UC活动期患者中TREM-1 mRNA的表达与ESR显著相关(r=0.582,P=0.03),而与血清中CRP水平不相关(r=0.447,P=0.055).结论 TREM-1mRNA的表达与UC的活动程度明显相关,TREM-1可能参与了UC的炎症进程.TREM-1 mRNA的表达水平可能与ESR相关,而与血清中CRP水平不相关.     

The value of serum CA 125 for the management of tubo-ovarian abscesses

OBJECTIVE: The aim of this study was to compare the value of CA 125, ESR and CRP in predicting the outcome of tubo-ovarian abscess (TOA) treatment and to investigate whether TOA treatment could be improved by following the dynamics of CA 125 serum levels. STUDY DESIGN: This prospective study included 36 patients with unilateral TOA, confirmed on the basis of history and physical examination, laboratory findings, and ultrasound (US) investigation. Venous blood samples for estimation of serum CA 125, CRP, and ESR were taken on hospital admission and then every 5 days. On day 20, the success of treatment was evaluated by clinical and sonographical re-assessment, and the patients were divided into two groups: those with complete regression of the TOA and those with residual inflammatory disease. Statistical analysis was performed by nonparametric Mann-Whitney U-test with multiple regression. RESULTS: On admission, CA 125 levels were increased (> 16.7 U/ml) in 32 patients (88.9%). The CA 125 level amounted to 55.7 U/ml on the average. A significant difference in CA 125 values between the above mentioned two groups was observed from day 10 of treatment onwards (p = 0.00015). On comparison of ESR, CRP, and CA 125, the latter had the highest predictive value for disease outcome after day 10 (p < 0.0011), while at the time of diagnosis, ESR proved more reliable in predicting the outcome. CONCLUSIONS: The dynamics of serum CA 125 allow an objective evaluation of the course and outcome of TOA, in combination with clinical parametres and US investigation.     

血清STIP-1、CA125a和CA125b诊断子宫腺肌病的临床价值

目的观察血清磷酸化应激诱导蛋白1(STIP-1)、CA125a和CA125b对子宫腺肌病的诊断价值。方法选择2018年1月至2019年12月在该院就诊、病理确诊为子宫腺肌病的患者93例为子宫腺肌病组。选择同期在该院健康体检者49例为健康对照组。观察两组研究对象血清STIP-1、CA125a、CA125b和CA125水平的变化及对子宫腺肌病的诊断效能;观察血清STIP-1、CA125a和CA125b水平与子宫腺肌病严重程度、痛经的关系,以及STIP-1、CA125a和CA125b之间的相关性。结果子宫腺肌病组患者血清STIP-1、CA125a、CA125b和CA125水平明显高于健康对照组(P<0.01)。血清STIP-1、CA125a和CA125b水平对子宫腺肌病的诊断效能明显优于CA125(P<0.05);STIP-1、CA125a、CA125b三者联合检测的灵敏度为94.6%,特异度为91.8%,受试者工作特征曲线下面积(AUC)为0.975,均明显高于STIP-1、CA125a和CA125b单项检测,而STIP-1、CA125a和CA125b3项单项检测的灵敏度、特异度、AUC差异均无统计学意义(P>0.05)。子宫腺肌病患者血清STIP-1、CA125a和CA125b水平随着病理分级升高而升高,有痛经的患者血清STIP-1、CA125a和CA125b水平高于无痛经的患者(P<0.05)。子宫腺肌病患者血清STIP-1水平与CA125a(r=0.761,P<0.01)和CA125b水平(r=0.814,P<0.01)呈正相关,而CA125a水平与CA125b水平呈正相关(r=0.786,P<0.01)。结论血清STIP-1、CA125a和CA125b参与了子宫腺肌病的病理生理过程,在诊断子宫腺肌病时具有较高的诊断效能,值得临床推广。     

Does the urinary excretion of α1-microglobulin and albumin predict clinical disease activity in ulcerative colitis?

Introduction  There remains some difficulty in determining disease activity during the development of inflammatory bowel disease (IBD). The excretion levels of some inflammatory response molecules increase as a result of the onset of this disease. We studied urinary alfa-1-microglobulin (α1-MG) and albumin levels in patients with active and inactive ulcerative colitis (UC) and investigated whether we could use these parameters as an activity index. Methods  The study was carried out at Gazi University Faculty of Medicine, Nephrology and Gastroenterology Departments, between December 2003 and March 2006. In total, 35 patients (male/female: 16/19, mean age: 38.3±2.4 years) and 13 healthy controls (male/female: 6/7, mean age: 35.8±2.8 years) were enrolled in the study. Nineteen patients had symptoms of active disease and the remaining 16 patients had inactive disease. Results  There was a significant difference in serum C-reactive protein (CRP), urinary albumin excretion, and α1-MG excretion levels between patients and controls. Patients with active disease had significantly higher serum CRP and α1-MG levels than those with inactive disease and controls. Patients with active disease had higher microalbuminuria levels than inactive patients, but this difference was not statistically significant. Urinary albumin and α1-MG excretion did not correlate with serum CRP levels. Conclusion  The present study suggests that, as with CRP, urinary levels of albumin and α1-MG increase during the active period of UC. During the inactive period, concentrations of these parameters are comparable to controls. The measurement of α1-MG and/or microalbuminuria could provide information on disease severity and response to treatment.