黄芩苷对人淋巴细胞Toll样受体的调节

目的:研究黄芩苷对人淋巴细胞Toll样受体(TLR)的调节,探讨其在免疫调节方面的作用。方法:1.尼龙毛分离法分离人外周血T和B细胞后使用UF-50流式分析寻找最佳黄芩苷作用浓度;2.采用实时定量荧光RT-PCR,分析在最佳黄芩苷作用浓度处理前后TLRs的表达情况;3.通过加入鼠抗人TLR4单抗进行受体抑制实验并应用实时定量荧光RT-PCR探讨黄芩苷对TLRs的调节位点。结果:1.1 mg/ml的黄芩苷在体外可以显著促进人T、B淋巴细胞增殖(P<0.05);2.使用1mg/ml黄芩苷相对于未用黄芩苷处理的T、B细胞,其TLR3、TLR7、TLR8及TLR9 mRNA的表达均有显著增加(P<0.05),T细胞增加倍数分别为21、15、57和66倍,B细胞增加的倍数分别为24、20、61和63倍,而TLR1、TLR2、TLR4、TLR5、TLR6及TLR10 mRNAs表达变化不显著(P>0.05);3.在1 mg/ml黄芩苷作用下人T、B细胞TLR3、7、8和9 mRNA在12小时时表达已开始增加,除T细胞的TLR7 mRNA表达在48小时时略有下降外,其余表达基本一直持续增加,至48小时到达峰值;4.人T、B细胞在被黄芩苷作用前使用鼠抗人TLR4单克隆抗体封闭,会显著降低黄芩苷对TLR3、7、8和9 mRNA表达的诱导作用(P<0.05),T细胞下降比率分别为47.6%、66.7%、50.9%和45.5%;B细胞下降比率分别为50.0%、45.0%、50.8%和50.8%。结论:1.黄芩苷在体外能显著促进T、B淋巴细胞增殖;2.人外周血T、B细胞组成性表达含量差异较大的TLR1-10 mRNA,黄芩苷在体外可以显著上调人T、B淋巴细胞TLR3、TLR7、TLR8及TLR9 mRNAs的表达,但对TLR1、TLR2、TLR4、TLR5、TLR6及TLR10 mRNAs未见明显影响;3.黄芩苷可能通过TLR4发挥对人天然免疫能力的调节。     

庆大霉素复合制剂治疗轻型复发性阿弗他溃疡的疗效观察

复发性阿弗他溃疡又名复发性口腔溃疡,其患病率居口腔黏膜病之首[1],主要表现为口腔黏膜上皮、固有层及黏膜下层的炎症反应.患者口腔内灼痛明显,发作轻者数月一次,重者连续发作,严重影响患者的生活质量.在临床上分为轻型、重型和疱疹样型3种.由于其病因复杂,目前还没有找到根治的特效方法.我们对2009年6月至2010年8月的患者使用由庆大霉素、地塞米松、维生素C和丁卡因等组成的复合制剂对轻型复发性阿弗他溃疡进行治疗,取得了较好的疗效,现报告如下.     

黄芩苷对CA46细胞裸鼠异种移植瘤的作用及机制

目的: 研究黄芩苷抗CA46细胞裸鼠异种移植瘤的作用并探讨其机制。方法: 构建CA46细胞裸鼠异种移植瘤模型,将荷瘤裸鼠随机分为5组:阴性对照组、15 mg/kg黄芩苷组、30 mg/kg黄芩苷组、60 mg/kg黄芩苷组和4 mg/kg依托泊甙 (VP-16) 阳性对照组。采用腹腔注射方式给药,12 d后处死部分裸鼠,对剥取的瘤块称重计算抑瘤率,并进行透射电镜、病理学和Western blotting检测;观察各组未处死的裸鼠直至全部死亡,研究黄芩苷对荷瘤裸鼠生存时间的影响。结果: 黄芩苷明显抑制了CA46细胞裸鼠异种移植瘤的生长,引起肿瘤细胞的凋亡、坏死以及p-Akt、NF-κB、mTOR和p-mTOR蛋白表达的下调;随着黄芩苷剂量的增加,黄芩苷治疗组荷瘤裸鼠的生存时间明显延长。结论: 黄芩苷可抑制CA46细胞裸鼠异种移植瘤的生长,诱导肿瘤细胞的凋亡,并延长荷瘤裸鼠的生存时间,其机制可能与下调PI3K/Akt/NF-κB和PI3K/Akt/mTOR信号通路有关。     

黄芩苷抑制CA46细胞增殖和诱导凋亡的作用机制探讨

目的： 研究中药黄芩苷（baicalin）对人Burkitt淋巴瘤细胞株CA46细胞增殖、凋亡的影响并探讨其可能作用机制。 方法：应用MTT法绘制细胞生长曲线观察黄芩苷对CA46细胞增殖的影响;Annexin V-FITC/PI双染流式细胞术、细胞DNA片段化、TdT酶介导的原位缺口末端标记(TUNEL)法检测黄芩苷诱导CA46细胞凋亡的能力;RT-PCR法检测黄芩苷作用前后c-myc、bcl-2 mRNA表达水平的变化,Western blotting法检测c-Myc、Bcl-2、procaspase-3(caspase-3前体)、PARP(多聚ADP核糖聚合酶)蛋白水平的变化。结果：细胞生长曲线结果显示黄芩苷能明显抑制CA46细胞增殖,半数抑制浓度(IC50)约为10 μmol/L;Annexin V-FITC/PI双染流式细胞术早期凋亡的检出、TUNEL晚期凋亡细胞的检出和细胞DNA片段化凋亡梯带的检出,均证实黄芩苷能有效诱导CA46细胞凋亡,细胞凋亡率呈现浓度依赖性递增。黄芩苷作用后CA46细胞c-myc、bcl-2 mRNA和c-Myc、Bcl-2、procaspase-3、PARP(116 kD)蛋白的表达呈现时间依赖性递减,而PARP(85 kD)表达呈现时间依赖性递增。结论：黄芩苷能有效抑制CA46细胞增殖,诱导其凋亡;c-Myc、Bcl-2表达水平下调和caspase-3激活可能参与了这一作用过程。     

黄芩苷调节巨噬细胞极化减轻脂多糖诱导的大鼠急性肺损伤

目的 研究黄芩苷对脂多糖(LPS)诱导急性肺损伤(ALI)的保护作用,并探讨该作用与肺巨噬细胞M1/M2表型极化及其介导炎症的相关机制.方法 将60只SD大鼠分为6组:正常对照组、LPS组、(10、50、100)mg/kg黄芩苷联合LPS处理组、地塞米松(DEX)处理组.除正常对照组外,其余组采用气管滴注LPS建立AL...     

黄芩清热除痹胶囊对强直性脊柱炎患者疗效及氧化应激的影响

目的:观察黄芩清热除痹胶囊(HQC)对强直性脊柱炎(AS)患者疗效及氧化应激的影响,并探讨其作用机制。方法:将60例AS患者随机分为治疗组和对照组各30例,治疗组予以黄芩清热除痹胶囊(HQC)口服,3次/d,3粒/次,对照组予以柳氮磺吡啶(SASP)口服,2次/d,4片/次,连服3个月,同时选择30名健康对照组。应用ELISA法检测血清SOD、TAOC、MDA、LPO、IL-1β、TNF-α、IL-4、IL-10,仪器法测定红细胞沉降率(ESR),全自动生化仪分析仪检测超敏C-反应蛋白(hsCRP),观察治疗前后VAS、BASDAI、BASFI、BAS-G、BASMI,评价HQC对AS患者的临床疗效。结果:与正常组相比,AS组TAOC、SOD、IL-4、IL-10水平明显降低,MDA、LPO、TNF-α、IL-1β明显升高(P 0. 01);与治疗前相比,两组治疗后VAS、BASDAI、BASFI、BAS-G、BASMI、ESR、hs-CRP明显下降(P 0. 01),与SASP组相比,HQC组BASDAI、hs-CRP显著降低,BASDAI50显著升高(P0. 05);与治疗前相比,两组IL-4、IL-10、TAOC、SOD均明显上升,TNF-ɑ、IL-1β、MDA、LPO明显降低(P0. 01或P0. 05),与SASP组相比,HQC组IL-10、SOD显著升高,MDA显著降低(P0. 05)。结论:HQC可有效改善AS患者临床症状及实验室指标,对AS有一定治疗作用,其机制可能与纠正细胞因子失衡、改善氧化应激状态、减轻氧化损伤有关。     

建立复发性口疮大鼠模型和黄芩苷的干预

背景：细胞外基质的降解与重构是多种病理及生理过程的共同通路,参与了多种口腔疾病的发病过程。目的：分析复发性口疮大鼠细胞凋亡的细胞外基质重构参与作用及黄芩苷的干预作用,为相关新药开发和临床治疗提供参考。方法：40只SD大鼠随机分为4组：对照组、复发性口疮模型组、小剂量黄芩苷组和大剂量黄芩苷组。后3组大鼠采用口腔黏膜匀浆组织加弗氏免疫佐剂建立大鼠复发性口疮模型。小剂量黄芩苷组和大剂量黄芩苷组大鼠分别给予10 mg/kg和100 mg/kg黄芩苷灌胃,1次/d,连续14 d。结果与结论：与对照组相比,模型组大鼠血清超氧化物歧化酶水平明显降低,而丙二醛水平明显增高,口腔黏膜中Bax、Caspase3和基质金属蛋白酶2/9表达水平增加,而Bcl-2表达水平降低。而小剂量黄芩苷组和大剂量黄芩苷组大鼠上述指标明显恢复,且不同剂量黄芩苷的影响差异有显著性意义。提示黄芩苷主要通过抑制细胞外基质降解与重构过程治疗复发性口疮。中国组织工程研究杂志出版内容重点：肾移植;肝移植;移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植;组织工程     

黄芩苷抑制NLRP3炎性小体与TLR4/NF-κB信号通路缓解感染性早产大鼠子宫平滑肌收缩

目的 探讨黄芩苷对LPS诱导的感染性早产大鼠子宫平滑肌的改善作用及可能机制.方法 将孕鼠分为空白组(Control)、感染性早产组(LPS)、黄芩苷低剂量组(Baicalin L)及黄芩苷高剂量组(Baicalin H),每组10只.检测子宫肌张力;HE观察子宫病理组织学变化;ELISA检测各组血清中SOD、CAT、MDA、IL-1β、IL-6和TNF-α含量;Western-blot检测NLRP3、pro-IL1β和pro-caspase 1以及TLR4/NF-κB信号通路的表达.结果 黄芩苷改善感染性早产大鼠子宫肌张力,减轻子宫病理损伤,降低炎症反应,抑制氧化应激;同时下调NLRP3、pro-IL1β和pro-caspasel表达水平,抑制TLR4/NF-KB信号通路蛋白表达水平.结论 黄芩苷缓解感染性早产大鼠子宫平滑肌收缩,与抑制NLRP3炎性小体与TLR4/NF-κB信号通路有关.     

中药制剂的质量控制因素与科学化分析

目的：就中药制剂的质量控制因素进行分析,并提出科学化的整改措施,以提高中药制剂产品的质量。方法对我院近年来中药制剂的制作及质量影响因素进行回顾性分析,并结合相关文献报道,寻找科学化的质控方案来提高中药制剂的质量。结果处方、制备工艺、贮存条件是影响中药制剂质量的重要因素。结论严格审核中药处方、加强环节管理可以有效的提高中药制剂的质量,这可为临床提供优质的中药制剂。     

清开灵眼用凝胶的制备与质量控制

研发清开灵眼用凝胶并对其质量进行控制。以羟丙甲纤维素（HPMC）主要基质刺成眼用凝胶,采用定性和定量方法进行质量控制,定量方法采用紫外可见分光光度仪测定其主要有效成分：黄芩苷和胆酸的含量。清开灵眼用凝胶成型性、澄明度好,且成本低,质量可控。本方法简便、准确、重现性好,可用于清开灵眼用凝胶的质量控制。     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

Recovery of responsiveness of cells of lateral geniculate nucleus of rat

1. Recovery of responsiveness of single cells in lateral geniculate nucleus of rat has been determined in both P and I cells. There are three types of recovery curve among P cells; (a) early recovery, (b) early partial recovery followed by depression and then complete recovery, (c) prolonged depression followed by cyclic recovery. Type (c) is by far the commonest recovery curve. In contrast to the spike in a P cell, the synaptic potential recovers to its full amplitude in about 20 msec. All I cells exhibit similar rapid recovery curves after a prolonged depression.2. Conditioning stimuli applied to visual cortex also produce a prolonged depression in most P cells but I cells can be re-excited at short intervals from cortex. Decortication does not prevent the prolonged depression of the multineuronal response produced by optic nerve stimulation.3. A neuronal model is proposed to explain these observations. It is supposed that I cells (interneurones) are innervated by axon collaterals of the P cells (principal cells, projecting to visual cortex) and that the I cells exert an inhibitory influence on the P cells.     

Modes of Inheritance of Errors of Refraction

Eighteen families in which both parents had refractions within the range of +4·0 D to −4·0 D and axial lengths seen in emmetropia (22·3-26·0 mm) showed coefficients of correlation of the order 0·5 indicative of polygenic inheritance. Such coefficients were seen for axial length (0·407) and for the cornea (0·487), but not for the lens (which is known to be yoked to the axial length). No such coefficients were seen in 19 families in which one of the parents had axial length outside the emmetropic range (nine families with long axes and 10 with short axes).

The pattern of polygenic inheritance for emmetropia (completely correlated optical components) and errors of refraction up to 4·0 D (inadequately correlated components: correlation ametropia) follows that seen in stature and other measurable characters. In contrast the high refractive errors with their abnormal axial lengths (component ametropia) are—like the extremes in stature—pathological anomalies with monofactorial inheritance.

     

Evaluation of antibacterial activity of extracts of five species of wood-colonizing fungi

Screening new organisms for antibacterial activity and searching for new antibacterial drugs is important due to the constant generation of new antibiotic-resistant strains of pathogenic bacteria. An E. coli broth microdilution test was used to evaluate the results of the Vibrio fischeri bioluminescence test in five of the most antibacterially active species of wood-colonizing fungi. Serpula lacrymans was found to be a potential source of thermostable antibiotic(s) and the Vibrio fischeri bioluminescence test was confirmed to be a useful method for screening for antibacterial activity.