食管鳞状细胞癌中CK7蛋白表达及预后意义

目的观察细胞角蛋白7(CK7)在食管鳞状细胞癌中的表达及其与预后的关系。方法采用免疫组化ELPS法检测512例食管鳞状细胞癌中CK7的表达,分析其表达与临床病理特征及患者预后的关系。结果 512例食管鳞状细胞癌中CK7蛋白阳性率为24.6%,CK7表达与肿瘤分化差、淋巴结转移和临床分期较晚相关(P0.05)。生存分析显示,CK7阳性患者的无瘤生存期(disease free survival, DFS)和总生存期(overall survival, OS)较短(DFS:P=0.016;OS:P=0.020)。多因素生存分析显示,pTNM分期是影响患者预后的独立危险因素。进一步分析发现,在Ⅰ+Ⅱ期食管鳞状细胞癌中,CK7阳性患者的DFS和OS较短(DFS:P=0.047;OS:P=0.023),Ⅲ+Ⅳ期食管鳞状细胞癌中,CK7阳性患者的DFS和OS与CK7阴性患者相比,差异无显著性(DFS:P=0.631;OS:P=0.824)。结论 CK7阳性食管鳞状细胞癌可能存在较高的恶性程度。在Ⅰ+Ⅱ期食管鳞状细胞癌中,CK7表达与患者预后差相关,此发现有助于对这部分患者进行预后的危险度分级。     

ALDH1在食管鳞状细胞癌中的表达及其临床意义

目的研究食管鳞状细胞癌组织中乙醛脱氢酶-1（ALDH1）的表达与食管鳞状细胞癌的生物学行为和预后之间的相关性。方法应用免疫组织化学链霉抗生物素蛋白-过氧化物酶连接法（SP法）分别检测80例术前无放、化疗食管鳞状细胞癌标本的ALDH1表达情况。结果根据Q评分（quick score）,按Q〉120为高表达的标准,80例食管鳞状细胞癌标本中有25例高表达ALDH1,高表达率为31.25%。研究显示ALDH1的表达与食管鳞状细胞癌患者年龄、性别、TNM分期、淋巴结转移均无明显的相关关系（P均〉0.05）,而与食管鳞状细胞癌的组织分化程度有关（P〈0.05）,食管鳞状细胞癌患者中ALDH1高表达组的生存率明显低于低表达组（P〈0.05）。结论食管鳞状细胞癌ALDH1的表达与组织分化程度及患者的预后有密切关系,ALDH1可作为食管鳞状细胞癌预后评价的参考指标。     

食管鳞状细胞癌中FOXM1基因的表达及临床意义

目的探讨FOXM1基因在食管鳞状细胞癌中的表达和临床意义以及下调FOXM1表达对人食管鳞状细胞癌细胞株KYSE-30增殖活性的影响。方法采用qRT-PCR及免疫组化技术检测FOXM1基因在食管鳞状细胞癌及癌旁组织中的表达;通过RNA干扰技术(RNAi)下调KYSE-30细胞FOXM1表达,CCK-8法检测细胞的增殖活性。结果 qRT-PCR结果显示,食管鳞状细胞癌中FOXM1 mRNA表达显著高于对应癌旁组织(P0.01);FOXM1 mRNA在低分化食管鳞状细胞癌组织中的表达量显著高于高+中分化食管鳞状细胞癌组织(P0.01);FOXM1 mRNA过表达与食管鳞状细胞癌肿瘤分化程度(P=0.001)、淋巴结转移(P=0.000)、临床分期(P=0.004)显著相关;高表达FOXM1食管鳞状细胞癌患者生存率下降(P0.001)。免疫组化结果显示,FOXM1蛋白在食管鳞状细胞癌中的表达显著增高并与其分化程度密切相关(P0.01);下调KYSE-30细胞中FOXM1表达后,细胞增殖速度受抑制(P0.01)。结论 FOXM1在食管鳞状细胞癌组织中呈高表达,并与食管癌分化程度、淋巴结转移、临床分期及预后密切相关,FOXM1可能参与调控人食管鳞状细胞癌细胞KYSE-30的增殖。     

长链非编码RNA CCHE1在胰腺导管腺癌中的表达及临床意义

目的检测长链非编码RNA(lncRNA)CCHE1在胰腺导管腺癌(PDAC)细胞和组织中的表达,探讨lncRNA CCHE1与PDAC临床病理特征及预后间的关系。方法RT-PCR检测PDAC组织及配对癌旁组织以及PDAC细胞和胰腺导管上皮细胞中lncRNA CCHE1的表达;利用单因素方差分析探讨lncRNA CCHE1与PDAC患者临床病理特征之间的关系;利用生存曲线分析lncRNA CCHE1与PDAC患者术后5年生存率的关系;利用Cox风险比例模型分析lncRNA CCHE1与PDAC患者预后的关系。结果lncRNA CCHE1在PDAC组织和细胞中均呈过表达;lncRNA CCHE1的表达与PDAC的分化程度以及TNM分期密切相关(P<0.05);lncRNA CCHE1高表达的PDAC患者术后无病生存时间和总生存时间均低于低表达的患者,lncRNA CCHE1高表达是PDAC患者预后的独立危险因素。结论lncRNA CCHE1在PDAC中表达升高,与PDAC的分化程度、TNM分期以及生存时间及预后相关。     

长链非编码RNA ELFN1-AS1在子宫内膜癌中的表达及其临床意义

目的 基于生物信息学探讨长链非编码RNA(lncRNA) ELFN1-AS1在子宫内膜癌(UCEC)中的表达及其临床意义。方法 从癌症基因组图谱(TCGA)数据库下载552例UCEC组织样本和35例癌旁组织样本的转录组测序(RNA-seq)数据及相关临床资料，基于R语言进行相关生物信息学分析，以解析ELFN1-AS1在UCEC中的表达及其与临床病理特征、预后及免疫细胞浸润的相关性，并评估其在UCEC中的诊断价值。结果 ELFN1-AS1在UCEC组织中的表达呈升高趋势(P<0.001)，其表达水平与肿瘤组织学分级、残瘤分级显著正相关(P均<0.05)，且高表达患者的总生存期(OS)、疾病相关生存期(DSS)及无进展间隔期(PFI)均显著短于低表达患者(P<0.05)，是影响UCEC患者OS的独立危险因素。肿瘤免疫细胞浸润分析显示，ELFN1-AS1与辅助性T细胞2(Th2)的浸润水平呈正相关，而与CD56^bright NK、中性粒细胞、嗜酸性粒细胞以及未成熟树突状细胞(iDC)的浸润呈负相关(|Spearman’s r|>0.15,P<...     

食管鳞状细胞癌组织中Id1和Id2的表达及其意义

目的研究Id1、Id2在食管鳞状细胞癌中的表达及其意义。方法采用免疫组织化学SP法和图像分析技术检测122例食管鳞状细胞癌及90例癌旁正常组织中Id1、Id2的表达情况。结果122例食管鳞状细胞癌中Id1、Id2表达高于癌旁正常组织(P0.01);Id1、Id2表达强度与患者的性别、年龄、淋巴结转移未见明显相关性(P0.05);Id1表达高分化与低分化组差异有显著性(P0.05);Id2的表达与肿瘤的分化程度成负相关(P0.05),且与肿瘤浸润深度正相关(P0.05)。结论Id1、Id2的高表达可能是食管鳞状细胞癌一个重要的分子学改变,Id2可作为判断食管鳞状细胞癌生物学行为的潜在指标。     

喉癌组织中LKB1与VEGF -C表达的相关性研究

目的:检测喉癌组织中抑癌基因LKB1和血管内皮生长因子VEGF-C的表达情况,探讨VEGF-C在喉鳞状细胞癌、正常组织中的表达关系.方法:选取喉癌患者40例喉癌组织为实验组,40例癌旁正常组织(距肿瘤边缘＞5mm,且病理证实为正常组织)为对照组.采用逆转录聚合酶链反应(RT-PCR)技术分析两组之间LKB1mRNA和VEGF-CmRNA的表达情况.结果:LKB1在癌组织中阳性表达率低于癌旁组织(P＜0.05),VEGF-C在癌组织中阳性表达率高于癌旁组织(P＜0.05),均与组织分化程度有相关性(P＜0.05).LKB1与VEGF-C呈直线负相关.结论:在喉鳞状细胞癌发生的过程中,LKB1和VEGF -C发生异常表达,且与喉鳞状细胞癌的进展有关.     

表皮生长因子受体和P53与食管鳞状细胞癌放疗敏感性的关系

目的 分析食管鳞状细胞癌患者标本中表皮生长因子受体(EGFR)和P53表达水平与其临床病理特征的相关性,探讨术前放疗对EGFR和P53表达的影响,为临床食管鳞状细胞癌手术联合放疗的治疗策略提供理论依据.方法 采用免疫组织化学方法检测食管鳞状细胞癌患者标本中EGFR、P53蛋白的表达水平,分析其表达与食管鳞状细胞癌临床病理参数的关系,对比术前放疗对患者癌组织中EGFR和P53表达水平的影响.结果 与正常食管黏膜上皮组织相比,食管鳞状细胞癌组织中EGFR和P53的表达水平均显著升高;食管鳞状细胞癌组织中EGFR和P53的表达均与其组织学分级、浸润程度、有无区域淋巴结转移呈正相关;术前放射治疗可显著降低食管鳞状细胞癌组织中EGFR和P53的表达水平.结论 在食管鳞状细胞癌中,EGFR和P53的表达水平与其临床病理特征有密切关系,且呈正相关,检测两种蛋白的表达水平对食管鳞状细胞癌的恶性程度及预后判断具有重要的临床意义.     

HINT1基因在喉鳞状细胞癌中的表达和意义

目的组氨酸三聚体核苷结合蛋白1(histidine triad nucleotide-binding protein1,HINT1)基因在喉鳞状细胞癌组织中的表达情况及其与患者临床病理特征的关系,探讨HINT1基因在喉鳞状细胞癌发病过程中的作用。方法通过实时定量PCR和免疫组织化学检测82例喉鳞状细胞癌组织和56例癌旁组织中HINT1mRNA和蛋白水平的表达,分析其蛋白表达与患者临床病理特征的关系。结果喉鳞状细胞癌组织HINT1mRNA水平显著低于癌旁组织(P<0.01),HINT1蛋白表达阳性率显著低于癌旁组织(P<0.05)。晚期喉鳞状细胞癌组织中HINT1蛋白表达显著低于早期喉鳞状细胞癌组织(P<0.05)。结论 HINT1在喉鳞状细胞癌中可能发挥抑癌基因的作用。     

食管鳞状细胞癌组织中Periostin、VEGF的表达及意义

目的：探讨Periostin、VEGF蛋白在食管鳞状细胞癌组织中的表达及意义。方法采用免疫组化SP法检测130例食管鳞状细胞癌组织及癌旁正常食管黏膜组织中Periostin、VEGF蛋白的表达。结果食管鳞状细胞癌组织中Periostin和VEGF的阳性率明显高于正常食管黏膜组织(P<0．05)。 Periostin表达与食管鳞状细胞癌浸润深度、淋巴结转移有显著相关性(P<0．05)。 VEGF表达与食管鳞状细胞癌分化程度、浸润深度和淋巴结转移有显著相关性(P<0．05)。 Periostin与VEGF表达呈正相关(P<0．05),66例食管鳞状细胞癌患者获得临床随访资料,随访时间1~48个月,Kaplan-Meier生存曲线分析显示,Peri-ostin阳性组患者的生存率明显低于 Periostin阴性组( P<0．05), VEGF阳性组患者的生存率明显低于 VEGF 阴性组( P <0．05)。结论 Periostin与VEGF表达密切相关,联合检测Periostin、VEGF可作为判定食管鳞状细胞癌侵袭、转移能力的客观指标,对食管鳞状细胞癌的预后判断具有重要意义。     

Expression of long non-coding RNA ZEB1-AS1 in esophageal squamous cell carcinoma and its correlation with tumor progression and patient survival

Background: LncRNA ZEB1-AS1 has been identified as a tumor oncogene in hepatocellular carcinoma. However, the clinical significance in esophageal squamous cell carcinoma (ESCC) is still unknown. The aim of this study was to explore ZEB1-AS1 expression levels and evaluated its clinical significance in ESCC patients. Methods: LNCRNA ZEB1-AS1 expression was determined by quantitative real-time PCR (QRT-PCR) in 87 pairs of ESCC specimens and adjacent non-tumor tissues. Then, the association of ZEB1-AS1 expression with clinicopathological factors or survival of ESCC patients were determined. Results: LNCRNA ZEB1-AS1 was found up-regulated in ESCC tissues compared to adjacent non-tumor tissues. Increased lncRNA ZEB1-AS1 expression was significantly associated with tumor grade, depth of invasion, and lymph node metastasis. Kaplan-Meier analysis revealed that ESCC patients with high ZEB1-AS1 expression had a poorer overall survival and disease-free survival. Furthermore, multivariate analysis suggested that ZEB1-AS1 expression was identified as an independent prognostic factor in patients with ESCC. Conclusion: These results indicated that lncRNA ZEB1-AS1 was associated with tumor progression and could be an independent prognostic factor for ESCC patients.     

Overexpression of long non-coding RNA UCA1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma

Introduction: Long non-coding RNAs (lncRNAs) have been shown to have important regulatory roles in cancer biology, and the lncRNA UCA1 is upregulated in several cancers such as bladder cancer, breast cancer and colorectal cancer, however, the contributions of UCA1 to esophageal cancer remain largely unknown. Methods: Expression levels of lncRNA UCA1 in esophageal squamous cell carcinoma (ESCC) patients and esophageal cancer cell lines were evaluated by quantitative real-time PCR (qRT-PCR), and its association with overall survival of patients was analyzed by statistical analysis. Small interfering RNA was used to suppress UCA1 expression in esophageal cancer cell line. In vitro assays were conducted to further explore its underlying roles in tumor progression. Results: The relative level of UCA1 was significantly higher in ESCC tissues compared to the adjacent non-tumor tissues, and remarkably higher expression of UCA1 was found in esophageal cancer cell lines compared with the immortalized esophageal epithelial cell line NE1. The ESCC patients with higher UCA1 expression had an advanced clinical stage and a poorer prognosis than those with lower expression. In vitro assays, our data indicated that downregulation of UCA1 decrease cell proliferation, migration, and invasion ability. Conclusions: lncRNA UCA1 might be considered as a novel molecule involved in ESCC progression, which provides a potential prognostic biomarker and therapeutic target.     

Increased expression of the lncRNA PVT1 promotes tumorigenesis in non-small cell lung cancer

Introduction: Non-small cell lung cancer (NSCLC) is the major cause of cancer death worldwide. Increasing evidence shows that long non coding RNAs (lncRNAs) are widely involved in the development and progression of NSCLC. lncRNA PVT1 in several cancers has been studied, its role in lung cancer remains unknown. Our studies were designed to investigate the expression, biological role and clinical significance of PVT1 in lung cancer. Methods: lncRNA PVT1 expression in 82 NSCLC tissues and 3 lung cancer cell lines was measured by quantitative Real-time PCR (qRT-PCR). Its association with overall survival of patients was analyzed by statistical analysis. RNA interference (RNAi) approaches were used to investigate the biological functions of PVT1. The effect of PVT1 on proliferation was evaluated by MTT, cell migration and invasion ability was evaluated by cell migration and invasion assays. Results: lncRNA PVT1 expression was significantly upregulated in NSCLC tissues and lung cancer cells when compared with corresponding adjacent normal tissues and normal bronchial epithelial cells. Increased PVT1 expression was significantly correlated with histological grade and lymph node metastasis. In addition, NSCLC patients with PVT1 higher expression have shown significantly poorer overall survival than those with lower PVT1 expression. And PVT1 expression was an independent prognostic marker of overall survival in a multivariate analysis. In vitro assays our results indicated that knockdown of PVT1 inhibited cell proliferation, migration, and invasion. Conclusions: Our data indicated that lncRNA PVT1 is significantly upregulated in NSCLC tissues and may represent a new biomarker and a potential therapeutic target for NSCLC intervention.     

Elevated expression of MDR1 associated with Line-1 hypomethylation in esophageal squamous cell carcinoma

Background: The aim is to discuss the relationship of Line-1 methylation and the MDR1 expression in esophageal squamous cell carcinoma (ESCC). Methods: We analyzed the methylation level of Line-1 by quantitative real-time MSP, and the expression of MDR1 by real-time RT-PCR in 310 ESCC and corresponding non-tumor tissues. Results: We found that the methylation index (MI) of Line-1 decreased from 0.90 in non-tumor tissues toward 0.78 in ESCC. The cumulative survival was significantly shorter in ESCC patients with MI ≤ 0.78 (34 months) than that in patients with MI > 0.78 (43 months). There was a statistical difference between MI ≤ 0.78 and MI > 0.78 cases with these clinicopathologic parameters (age, AJCC stage, differentiation; P = 0.010, P < 0.0001, P = 0.015, respectively). These results implied that Line-1 hypomethylation could be more in ESCC patients with older, advanced tumor and poor differentiation group. Meanwhile, ESCC with demethylation of Line-1 were shown elevated MDR1 expression in tumor (Mean_-∆∆Ct = 0.21), but ESCC with hypermethylation of Line-1 were considered to be decreased MDR1 expression in tumor (Mean_-∆∆Ct = -0.86). Conclusions: Line-1 hypomethylation could be as a biomarker of poor prognosis in ESCC patients. MDR1 gene could be activated via epigenetic mechanisms with demethylation of Line-1 in ESCC, and enhance tumor progression.     

Expression and prognostic value of FOXP1 in esophageal squamous cell carcinoma

BackgroundForkhead box protein P1 (FOXP1) has been suggested as a prognostic marker in several malignant tumors. However, the significance of FOXP1 in esophageal squamous cell carcinoma (ESCC) is still unclear. The purpose of this study was to investigate the expression pattern of FOXP1 in normal esophageal tissue and ESCC and to analyze the clinicopathological significance and prognostic value of FOXP1 in ESCC.MethodsFOXP1 was detected by immunohistochemistry using tissue microarrays containing tumor tissues and adjacent normal tissues from 270 ESCC patients with oncological follow-up data.ResultsNormal esophageal tissues predominantly showed an exclusive nuclear FOXP1 (n-FOXP1) expression pattern, and no exclusive cytoplasmic FOXP1 (c-FOXP1) staining was found. In ESCC, the expression rates of exclusive n-FOXP1-positive, exclusive c-FOXP1-positive, both nuclear and cytoplasmic positive and complete negative were 14.4%, 28.9%, 10.4% and 46.3%, respectively. High n-FOXP1 expression was significantly correlated with decreased postoperative recurrence and distant metastasis (P < 0.05). Furthermore, elevated c-FOXP1 expression was significantly associated with regional lymph node metastasis and distant metastasis (P < 0.05). High c-FOXP1 expression had an effect on shorter overall survival (OS) time, but the difference was not statistically significant (P > 0.05). Kaplan–Meier analysis showed that ESCC patients with high n-FOXP1 expression survived significantly longer than patients with low n-FOXP1 expression. Multivariate analysis confirmed that patients with high n-FOXP1 staining exhibit good prognosis and n-FOXP1 was an independent factor for ESCC prognosis.ConclusionsOur results suggest that FOXP1 plays an essential role in ESCC progression and prognosis and may be a useful biomarker for predicting survival.     

Overexpression of long non-coding RNA CCAT1 is a novel biomarker of poor prognosis in patients with breast cancer

Introduction: Recent studies have demonstrated that lncRNA CCAT1 was increased in many types of cancers and was involved in various cellular processes related to carcinogenesis. However, the clinical significance and prognostic value of lncRNA CCAT1 in breast cancer (BC) haven’t been investigated. Methods: Expression levels of lncRNA CCAT1 in 92 pairs of BC cancer tissues and adjacent normal tissues were detected by quantitative real-time PCR. In order to determine its prognostic value, overall survival and progression-free survival were evaluated using the Kaplan-Meier method, and multivariate analysis was performed using the Cox proportional hazard analysis. Results: Expression levels of lncRNA CCAT1 in BC tissues were significantly higher than those in adjacent normal tissues. High expression of lncRNA CCAT1 was associated with differentiation grade, TNM stage, and lymph node metastases. Kaplan-Meier analysis with the log-rank test indicated that high expression of lncRNA CCAT1 had a decreased overall survival and progression-free survival. Multivariable analysis was further identified high expression of lncRNA CCAT1 as an independent prognosis factor for overall survival and progression-free survival. Conclusions: Our findings provided that the expression of lncRNA CCAT1 was up-regulated in BC and associated with overall survival as well as progression-free survival, suggesting that lncRNA CCAT1 could be a potential prognostic biomarker for BC progression.     

Up-regulation of long non-coding RNA CCAT2 correlates with tumor metastasis and poor prognosis in cervical squamous cell cancer patients

Background: Dysregulation of long non-coding RNAs (lncRNAs) plays critical roles in tumor progression. The purpose of this study was to investigate the relationship between lncRNA CCAT2 expression and cervical squamous cell cancer susceptibility and prognosis. Methods: Expression levels of lncRNA CCAT2 in 123 cervical squamous cell tumor specimens were determined by quantitative real-time PCR (qRT-PCR), to clarify the clinical significance of lncRNA CCAT2 in cervical squamous cell cancer, we further discussed the relationship between lncRNA CCAT2 expression and overall survival (OS) and progression-free survival (PFS). Results: In the present study, we found that lncRNA CCAT2 was up-regulated in cervical squamous cell cancer tissues compared to the adjacent non-tumor tissues. In addition, the high lncRNA CCAT2 expression was significantly associated with the FIGO stage, lymph node metastasis and depth of cervical invasion (P<0.05). Furthermore, patients with high expression of lncRNA CCAT2 had poor OS (HR=2.813, 95% CI: 1.504-6.172; P=0.017), and PFS rates (HR=3.072, 95% CI: 1.716-8.174; P=0.008). Multivariate Cox proportional hazard model analysis demonstrated that high lncRNA CCAT2 expression was an independent poor prognostic factor for cervical squamous cell cancer patients. Conclusions: Our study suggested that high expression of lncRNA CCAT2 is related to the prognosis of cervical squamous cell cancer; it may be a new prognostic biomarker and potential therapeutic target for cervical squamous cell cancer intervention.     

Mnk2蛋白水平与食管鳞癌预后的相关性

目的:检测丝裂原活化蛋白激酶相互作用激酶2(mitogen-activated protein kinase-interacting kinase-2,Mnk2)在食管鳞状细胞癌中的表达水平,并探讨其与患者生存预后的相关性。方法:收集临床食管鳞癌标本86例及癌旁正常食管组织54例,应用Western blot法和免疫组化SP法检测肿瘤组织及正常食管黏膜组织中Mnk2蛋白表达水平,并用Kaplan-Meier生存曲线和Cox比例风险回归模型的方法探究其与食管鳞癌患者预后的关系。结果:Mnk2在食管癌组织中呈高表达,并且Mnk2蛋白表达与食管鳞癌的TNM分期密切相关(P0.05),同时Mnk2蛋白高表达组的无疾病进展生存期和总生存期均少于Mnk2低表达组,多因素分析提示Mnk2是食管鳞癌的独立预后因子。结论:Mnk2在食管鳞癌组织中的表达与TNM分期有关,同时可作为预测食管鳞癌患者预后的指标。