IL-18、TNF-α、VEGF在宫腔粘连患者子宫内膜中的表达及临床意义

目的探讨白介素-18(IL-18)、肿瘤坏死因子-α(TNF-α)及血管内皮生长因子(VEGF)在宫腔粘连患者子宫内膜中的表达及临床意义。方法收集我院2010年1月~2013年收治的经宫腔镜检查确诊的宫腔粘连患者80例进行回顾性分析,80例子宫粘连患者中重度粘连者40例,中度粘连者40例,另选择同期行宫腔镜下子宫纵膈切除术的非宫腔粘连患者40例作为对照组,三组患者均采用免疫组化SP法染色检测IL-18、TNF-α及VEGF在子宫内膜组织中的表达。结果重度粘连组子宫内膜中IL-18、TNF-α及VEGF的表达高于对照组及中度粘连组,差异有统计学意义(P0.05);中度粘连组中IL-18、TNF-α及VEGF的表达高于对照组,差异有统计学意义(P0.05)。结论白介素-18(IL-18)、肿瘤坏死因子-α(TNF-α)及血管内皮生长因子(VEGF)均参与宫腔粘连的发生发展。     

宫腔粘连与子宫内膜雌激素受体和孕激素受体的表达特点及意义分析

目的：探讨宫腔粘连与子宫内膜雌激素受体和孕激素受体的表达特点及意义。方法25例宫腔粘连患者为观察组,25例患者子宫内膜正常部位为对照组。分析比较两组患者子宫内膜雌激素受体和孕激素受体的表达情况。结果观察组患者子宫内膜雌激素受体表达阳性率明显高于对照组,两组相比较差异具有统计学意义（P〈0.05）;观察组患者子宫内膜孕激素受体表达阳性率与对照组相当,两组相比较差异无统计学意义（P〉0.05）。结论通过研究表明,宫腔粘连患者子宫内膜雌激素受体表达阳性率较高,雌激素受体可能与宫腔发生粘连有联系。同时,为临床宫腔粘连的治疗提供了重要的参考价值。     

NF-κB和HBD-2的表达情况与宫腔粘连的相关性分析

目的 探讨核因子κB(NF-κB)和β-防御素2(HBD-2)表达与宫腔粘连的关系.方法 选取治疗的51例宫腔粘连患者的子宫内膜作为观察组,同时选取42例正常内膜组织作为对照组,采用免疫组织化学检测2组NF-κB和HBD-2表达水平,RT-PCR检测NF-κB mRNA和HBD-2 mRNA表达.结果 NF-κB在观察组中的高表达比例为80.39％明显高于对照组的11.90％,差异有统计学意义(P＜0.05);HBD-2在观察组中的高表达比例为94.12％,明显高于对照组的16.67％,差异有统计学意义(P＜0.05);不同程度宫腔粘连患者NF-κB和HBD-2高表达差异无统计学意义(P＞0.05);观察组NF-κB mRNA与HBD-2 mRNA相对表达量分别为(9.56±2.27)和(9.60±2.31),明显高于对照组,差异有统计学意义(P＜0.05).结论 NF-κB和HBD-2在宫腔粘连内膜组织中呈高表达,两者可能与宫腔粘连的发生发展有关.     

奈达铂同步化疗对中晚期宫颈癌患者uPA、VEGF及Ki-67表达的影响

目的 探讨奈达铂同步化疗对中晚期宫颈癌患者uPA、VEGF及Ki-67表达的影响.方法 收治的80例原发性宫颈癌患者随机分为观察组和对照组,每组40例.观察组采用奈达铂同步化疗治疗,对照组采用单纯放疗治疗,比较2组患者治疗效果及治疗前后uPA、VEGF和Ki-67表达阳性率变化.结果 观察组治疗总有效率为95骀.0%显著高于对照组的80.0%(P<0.05);随访期2组患者生存率、复发率和远处转移率差异有统计学意义(P<0.05);宫颈癌患者癌变组织中uPA、VEGF和Ki-67表达阳性率显著高于正常宫颈组织,差异有统计学意义(P<0.05);2组患者治疗后uPA、VEGF和Ki-67表达阳性率显著下降(P<0.05),且观察组下降程度显著高于对照组(P<0.05);观察组患者奈达铂同步化疗Ki-67阳性组化疗有效率显著高于阴性组,差异有统计学意义(P<0.05);观察组与对照组不良反应率比较差异无统计学意义(P>0.05).结论 奈达铂同步化疗能够显著降低中晚期宫颈癌患者宫颈组织中uPA、VEGF和Ki-67表达阳性率,提高中晚期宫颈癌患者治疗疗效,且患者能够耐受相关不良反应,值得临床推广使用.     

复发性鼻窦炎鼻息肉患者行修正性手术及综合治疗对VEGF、COX-2阳性率的影响

目的:探讨复发性鼻窦炎鼻息肉患者行修正性手术及综合治疗对VEGF、COX-2阳性率的影响。方法:选定某院于2017年7月～2019年3月收诊的74例复发性鼻窦炎鼻息肉患者,等距抽样法分为观察组(修正性手术及综合治疗)与对照组(传统手术)各37例,比较两组复发性鼻窦炎鼻息肉患者的临床有效率、环氧酶-2(COX-2)阳性率、血管内皮生长因子(VEGF)阳性率与并发症发生率。结果:观察组复发性鼻窦炎鼻息肉患者的COX-2阳性率、VEGF阳性率与鼻腔粘连、眼睑气肿等并发症的发生率均低于对照组(P0.05);观察组复发性鼻窦炎鼻息肉患者的临床有效率高于对照组(P0.05)。结论:修正性手术及综合治疗方法可提高复发性鼻窦炎鼻息肉患者的治疗效果与诊治安全性,减低其COX-2阳性率、VEGF阳性率,值得使用推广。     

宫腔镜术后加用自拟中药方对宫腔粘连患者受孕成功率的影响

目的 探究在宫腔镜术后加用自拟中药方对宫腔粘连患者受孕成功率的影响.方法 选取2018年1月至2019年12月南昌市医学科学研究院收治的宫腔粘连患者80例患者作为研究对象,依据随机图表法分为对照组(40例)与观察组(40例),对照组患者采用宫腔镜术后联用常规西药治疗,观察组采用宫腔镜术后联合自拟中药方治疗.比较两组患者的治疗效果、粘连程度与受孕率.结果 观察组治疗总有效率、受孕成功率均高于对照组,差异有统计学意义(P＜0.05).治疗前两组宫腔粘连程度评分比较,差异无统计学意义(P＞0.05),治疗后两组宫腔粘连程度评分均低于治疗前,且治疗后观察组宫腔粘连程度评分低于对照组,差异有统计学意义(P＜0.05).结论 于宫腔镜术后加用自拟中药方,可有效改善宫腔粘连患者病情,减轻患者粘连程度,显著提高受孕成功率,值得推广.     

吡柔比星联合顺铂膀胱灌注化疗对早期膀胱癌VEGF和HER-2表达的影响

目的 探讨吡柔比星联合顺铂膀胱灌注化疗对早期膀胱癌血管内皮生长因子(VEGF)、人类表皮生长因子受体-2 (HER-2)表达的影响.方法 收治的早期膀胱癌患者80例,参照抽签法分为对照组和试验组,每组40例.2组患者均于术后进行化疗,对照组给予顺铂常规化疗,研究组基于对照组联合吡柔比星行膀胱灌注化疗,比较2组患者治疗后VEGF水平、HER-2表达情况,生活质量变化,临床疗效,复发率及生存率,毒副反应.结果 治疗后,试验组VEGF低于对照组,比较差异有统计学意义(P＜0.05);试验组HER-2低表达率、过表达率优于对照组,差异有统计学意义(P＜0.05);试验组生活质量优于对照组(P＜0.05);试验组客观缓解率为87.50％高于对照组的62.50％,差异有统计学意义(P＜0.05);试验组复发率、生存率优于对照组(P＜0.05);2组毒副反应比较差异无统计学意义(P＞0.05).结论 吡柔比星联合顺铂膀胱灌注化疗能够显著改善早期膀胱癌患者VEGF水平,调节HER-2表达,提高患者的生活质量.     

中重度宫腔粘连患者宫腔镜术后二次探查的临床价值

目的探讨中重度宫腔粘连患者宫腔镜术后二次探查的临床价值。方法选取我院2015年1月～2017年1月接收的50例中重度宫腔粘连患者作为此次研究对象,入院确诊后均行宫腔镜下宫腔粘连分解术(TCRA),按照随机数字表法将其分为对照组与观察组,各25例,两组术后人工周期连续治疗3个月,后行宫腔镜检查了解术后宫腔情况,并以此为最终结论。观察组于术后第一次月经干净第3～7天行二次宫腔探查,对照组未行宫腔镜二次探查。对比两组临床价值。结果治疗前,两组患者子宫内膜增长及月经量水平对比差异无统计学意义(P 0.05);治疗后观察组子宫内膜及月经量明显高于(多于)对照组,差异有统计学意义(P 0.05);统计两组术后3个月后宫腔再粘连发生率得出观察组明显低于对照组,差异有统计学意义(P 0.05)。结论为中重度宫腔粘连行宫腔粘连分解术后二次宫腔镜探查,有助于了解患者宫腔恢复情况,及时采取相应措施处理,从根源上预防宫腔再粘连,值得在临床推广应用。     

宫腔粘连治疗中应用雌激素联合氧化再生纤维素防粘连膜的疗效分析

目的 探讨雌激素联合氧化再生纤维素防粘连膜在宫腔粘连治疗中的临床效果.方法 收集2015年1月至2015年8月入院的80例宫腔粘连患者随机分为两组,对照组患者给予雌激素治疗,实验组患者则加施氧化再生纤维素防粘连膜干预,比较两组患者治疗前后月经量与子宫内膜厚度、宫腔粘连总体治疗效果、粘连复发率、药物不良反应与妊娠情况.结果 实验组患者治疗后3个月月经量为(52.82±8.50),子宫内膜厚度为(8.59±0.34),显著高于对照组的(34.33±7.27)、(8.03±0.45),差异具有统计学意义(均P＜ 0.01);实验组患者治疗总有效率(100.00％)明显高于对照组(87.50％),差异具有统计学意义(P＜0.05);实验组患者粘连复发率(10.00％)明显低于对照组(31.43％),差异具有统计学意义(P＜0.05);两组患者药物不良反应率(12.50％比7.50％)比较差异无统计学意义(P＞0.05).结论 雌激素联合氧化再生纤维素防粘连膜在宫腔粘连治疗中的临床效果显著,安全性较高,借鉴意义重大.     

透明质酸钠对宫腔手术患者围术期微循环及粘连状态的影响观察

目的观察透明质酸钠对宫腔手术患者围术期微循环及粘连状态的影响。方法选取2009年1月~2012年1月于本院进行宫腔手术治疗的74例患者为研究对象,将其根据随机数字表法分为对照组37例与观察组37例。对照组进行常规用药,观察组则在对照组的基础上加用透明质酸钠。比较两组患者的总粘连率及手术前后的血清FN、LN、TGF-β、VEGF、宫颈微循环指标。结果观察组的总粘连率为5.41%（2/37）,对照组为13.51%（5/37）,两组比较,差异有统计学意义（P〈0.05）;手术后2、4周,观察组的血清FN、LN、TGF-β、VEGF水平均低于对照组,观察组手术后4周的上述指标水平高于手术后2周（P均〈0.05）;手术后2、4周,观察组的宫颈血流量与宫颈血管清晰率均高于对照组（P均〈0.05）。结论透明质酸钠可有效预防宫腔手术术后粘连,可明显改善患者的围术期微循环及粘连状态。     

Efficacy of gut lavage,hemodialysis, and hemoperfusion in the therapy of paraquat or diquat intoxication

Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.

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Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.

     

In vitro studies on sterically stabilized liposomes (SL) as enzyme carriers in organophosphorus (OP) antagonism

This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine.     

Steroidal sapogenin contents in some domestic plants

In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins.     

Synthesis,Cytotoxicity and Antileishmanial Activity of Some N-(2-(indol-3-yl)ethyl)-7-chloroquinolin-4-amines

We report herein the condensation of 4,7-dichloroquinoline (1) with tryptamine (2) and D-tryptophan methyl ester (3) . Hydrolysis of the methyl ester adduct (5) yielded the free acid (6) . The compounds were evaluated in vitro for activity against four different species of Leishmania promastigote forms and for cytotoxic activity against Kb and Vero cells. Compound (5) showed good activity against the Leishmania species tested, while all three compounds displayed moderate activity in both Kb and Vero cells.     

Aquatic Insects and Trace Metals: Bioavailability,Bioaccumulation, and Toxicity

Abstract

The uptake of metals from food and water sources by insects is thought to be additive. For a given metal, the proportions taken up from water and food will depend both on the bioavailable concentration of the metal associated with each source and the mechanism and rate by which the metal enters the insect. Attempts to correlate insect trace metal concentrations with the trophic level of insects should be made with a knowledge of the feeding relationships of the individual taxa concerned. Pathways for the uptake of essential metals, such as copper and zinc, exist at the cellular level, and other nonessential metals, such as cadmium, also appear to enter via these routes. Within cells, trace metals can be bound to proteins or stored in granules. The internal distribution of metals among body tissues is very heterogeneous, and distribution patterns tend to be both metal and taxon specific. Trace metals associated with insects can be both bound on the surface of their chitinous exoskeleton and incorporated into body tissues. The quantities of trace meals accumulated by an individual reflect the net balance between the rate of metal influx from both dissolved and particulate sources and the rate of metal efflux from the organism. The toxicity of metals has been demonstrated at all levels of biological organization: cell, tissue, individual, population, and community. Much of the literature pertaining to the toxic effects of metals on aquatic insects is based on laboratory observations and, as such, it is difficult to extrapolate the data to insects in nature. The few experimental studies in nature suggest that trace metal contaminants can affect both the distribution and the abundance of aquatic insects. Insects have a largely unexploited potential as biomonitors of metal contamination in nature. A better understanding of the physico-chemical and biological mechanisms mediating trace metal bioavailability and exchange will facilitate the development of general predictive models relating trace metal concentrations in insects to those in their environment. Such models will facilitate the use of insects as contaminant biomonitors.