窝沟釉质成形术对封闭剂渗透性及密合性的影响

目的 通过观察封闭剂的渗透性和密合性，进一步探讨窝沟釉质成形封闭术的作用机制。方法 取离体牙６０颗，分为Ａ、Ｂ、Ｃ３个组，Ａ、Ｂ两组分别用窝沟釉质成形封闭术（ｅｎａｍｅｌｏｐｌａｓｔｙ ｓｅａｌａｎｔ ｔｅｃｈｎｉｑｕｅ，ＥＳＴ）和窝沟杯刷清洗封闭术（ｃｕｐ－ｓｈａｐｅｄ ｂｒｕｓｈ ｓｅａｌａｎｔ ｔｅｃｈｎｉｑｕｅ，ＣＳＴ），Ｃ组作为对照组，进行酸蚀、隔湿干燥后涂封闭剂光照。采用染料浸染法和体     

癌基因c－erbB－2、抗癌基因p53在涎腺良恶性肌上皮瘤中的表达及分析

应用免疫组化方法对４０例涎腺肌上皮瘤（ＭＥ）、肌上皮瘤细胞生长活跃（ＭＡＰ）、恶性肌上皮瘤（ＭＭＥ）进行了ｃ－ｅｒｂＢ－２、ｐ５３基因蛋白表达的研究。结果：ｃ－ｅｒｂＢ－２表达总的阳性率为４２．５％（１７／４０），其中ＭＥ为１６．７％（２／１２）．ＭＡＰ为３７．５％（３／８），ＭＭＥ为６０％（１２／２０），三者间有显著差异（Ｐ＜０．０５），ｐ５３表达总的阳性率为２５％（１０／４０），ＭＥ，ＭＡＰ，ＭＭＥ三者间无显著差异（Ｐ＞０．０５）．ｃ－ｅｒｂＢ－２与ｐ５３在该瘤中的表达无相关性，结果表明ｃ－ｅｒｂＢ－２和ｐ５３的过量表达可能参与了涎腺肌上皮瘤的发生和分化过程；特别是癌基因ｃ－ｅｒｂＢ－２的检测对鉴别该瘤的良、恶性及早期诊断有一定的意义。     

传代的人牙髓成纤维细胞在体外培养下的增殖和表型变化

观察和比较了在ＤＭＥＭ加１０％ＦＣＳ；ＤＭＥＭ加１０％ＦＣＳ和５０μｇ／ｍｌＶｉｔＣ和１０ｍｍｏｌ／Ｌβ－磷酸甘油钠两种培养条件下，第５代人牙髓细胞的某些生物学特征，观察时间为３、９、１５、２１ｄ，通过ＣｏｎＡ和ＷＧＡ凝集素受体表达观察细胞的分化情况。结果表明第５代的人牙髓细胞可能是单一基因表型的细胞，其多层生长的能力减弱，牙髓细胞的凝集素受体（ＣｏｎＡ和ＷＧＡ）表达随培养时间延长未见明显改变。说明随着人牙髓细胞传代培养，人牙髓细胞的某些生物学特征发生了变化。     

热固化义齿软衬材料与基托树脂粘结的实验研究

测试了不同底胶、软衬材料中ＥＤＭＡ的含量及ＰＭＭＡ表面预处理对软衬材料与ＰＭＭＡ粘结强度的影响。结果表明，底胶Ｃ粘结效果最好，含有５％ＥＤＭＡ的软衬料与ＰＭＭＡ粘结强度最大，ＰＭＭＡ表面经处理后，粘结强度有明显提高。     

颌面部软组织韧带状瘤误诊3例报道

颌面部软组织韧带状瘤误诊３例报道ＴＨＲＥＥＣＡＳＥＯＦＤＥＳＭＯＩＤＴＵＭＯＲＩＮＭＡＸＩＬＬＯＦＡＣＩＡＬＲＥＧＩＯＮ陈燕杨威赵桂香韧带状瘤（ＤｅｓｍｏｉｄＴｕｍｏｒ）又称肌腱膜纤维瘤病、侵袭性纤维瘤病等，多发生在腹壁，颌面部甚为少见，常易误诊。现...     

Nd：YAG激光照射釉质,牙本质和牙骨质的形态变化

应用不同能量的脉冲Ｎｄ：ＹＡＧ激光照射釉质、牙本质和牙骨质表面，用ＳＥＭ观察其形态变化。结果表明：这些组织的表面产生许多密集、形态和边缘不规则的凹窝结构，深度１５～４０μｍ不等，但３种组织表面形态的改变和程度各不相同。分析讨论了粗糙的表面对复合树脂粘结的潜在意义，并提出合适的激光能量参数     

几株口腔厌氧菌胞外膜泡,外膜蛋白复合物的提取

采用盐析、超滤、超速离心等方法提取并比较了５种口腔常见厌氧菌（ＰｇＷ５０，Ｐｇ３８１，ＰｅＡＴＣＣ３５４０６，ＰｉＡＴＣＣ２５２６１，ＰｍＡＴＣＣ２５８４５）的胞外膜泡（ＥＣＶ）、外膜蛋白复合物（ＯＭＰＣ），结果显示：二种提取物外观性状均较好，呈淡灰色，其中ＰｇＷ５００ＯＭＰＣ的湿沉淀有一定粘度。ＥＣＶ的提取率由高至低分别为：ＰｇＷ５０（１９．８４ｍｇ／Ｌ）、Ｐｇ３８１（１８．７５ｍｇ／Ｌ）、Ｐｅ（１５．６５ｍｇ／Ｌ）、Ｐｍ（９．６０ｍｇ／Ｌ）、Ｐｉ（７．４０ｍｇ／Ｌ）；ＯＭＰＣ提取率分别为：ｐｇＷ５０（８７．３６ｍｇ／Ｌ）、Ｐｇ３８１（７２．５６ｍｇ／Ｌ）、Ｐｉ（５７．４０ｍｇ／Ｌ）Ｐｍ（４１．２８ｍｇ／Ｌ）、Ｐｅ（３２．７６ｍｇ／Ｌ）．在ＥＣＶ的提取中，采用超滤和超离心法提取ＥＣＶ的量要高于盐析法，表明营养条件不是细菌ＥＣＶ形成的决定因素。     

儿童口腔粘膜原发恶性黑色素瘤1例

儿童口腔粘膜原发恶性黑色素瘤１例ＡＣＡＳＥＯＦＭＡＬＩＧＮＡＮＴＭＥＬＡＮＯＭＡＩＮＣＨＩＬＤＯＲＡＬＭＵＣＯＳＡ张俊明作者单位：天津市静海县中医院口腔科（３０１６００）口腔粘膜恶性黑色素瘤较为少见，约占所有恶性黑色素瘤的０．４％～１．４％［１］此瘤...     

细菌膜泡、外膜蛋白复合物的十二烷基磺酸钠－聚丙烯凝胶电泳分析

本实验用连续测定法观察了细菌的生长率的变化，结果表明：三株卟啉菌在３ｄ的生长率基本相似，均以指数方式生长，其中ＰｇＷ５０在以后的几天中仍基本保持平衡，Ｐｇ３８１在５～６ｄ后开始呈下降趋势，而Ｐｅ在３～４ｄ到达顶点后，即开始呈下降趋势。ＳＤＳ－ＰＡＧＥ显示：ＥＣＶ的泳带要比同菌ＯＭＰＣ的泳带多，且大多集中在２０ＫＤ到１００ＫＤ之间．各带的含量（峰高）亦不相同。     

窝沟封闭剂用于正畸儿童的临床观察

窝沟封闭剂用于正畸儿童的临床观察朱红马炳岿杜辉目前我国儿童的龋齿发病率仍然很高，据调查６～１２岁儿童龋齿发病率达４０～５０％，其中面窝沟龋占８０％以上．在我科接受正畸治疗的患儿中，有一半以上的孩子治疗前已出现不同程度的第一恒磨牙龋．由于戴入矫正器后...     

窝沟封闭剂的实验研究

参照美国封闭剂Concise配方组成,自制了可见光固化和自凝两种封闭剂.实验室研究结果表明,在粘结性能、吸水性和磨耗率方面,自制封闭剂和Concise无差异.在表面硬度值方面,两种自制封闭剂均极显著低于相应固化方式的Concise(P<0.01).在抗压强度方面,自制光固化封闭剂与光固化Concise无差异;自制自凝封闭剂则显著低于自凝Concise.在径向抗张强度方面,自制光固化封闭剂显著高于光固化Concise;而自制自凝封闭剂极显著低于自凝Concise.总体评价是:自制封闭剂的性能和Concise大体相似.     

不同固化方式对封闭剂性能的影响

考察光固化、化学固化两种固化方式对封闭剂性能的影响。从粘结性能看，粘度值相近，树脂突长度无显著差异，但抗剪粘结强度光固化优于化学固化。在机械性能上，自制材料表面硬度、径向抗张强度光固化高于化学固化，在抗压强度上两者差异不大（Ｐ＞０．０５），但Ｃｏｎｃｉｓｅ材料结果与此相反，原因有待进一步研究，吸水性和磨耗率都是光固化小。总体评价，光固化实验室性能优于化学固化。     

Retention of a fissure sealant with caries reduction in Finnish children after six months

abstract — The purpose of this study was to test the retention as well as the caries reduction potential of a fissure sealant under Finnish dietary conditions. The 150 children who served as subjects were selected on the basis of having at least one pair of sound permanent molars. Two hundred first molars, 43 premolars and 119 primary molars were sealed, while the respective tooth from the other side of the jaw was left untreated and used as the control. The findings after 6 months revealed that all 362 sealants persisted. Among the sealants of the first permanent molars, 99% were in excellent condition. All sealants of the permanent premolars and of the primary molars were excellent. Among the permanent control molars 43 (22%) showed carious fissures while among the sealed permanent molars, only 3 (1.5%) had carious fissures. This gives a 93% reduction of the fissure caries. Both the control and the sealed premolars were all sound. None of the sealed primary molars were decayed compared to 7.6% decayed on the control side.     

Er：YAG激光对儿童窝沟封闭疗效影响的临床观察

目的：将Er：YAG激光应用于年轻恒牙窝沟封闭术,比较封闭剂的总保留率。方法：选择6～9岁、双侧下颌第一磨牙窝沟形态复杂的儿童60例为研究对象,随机分为A、B两组,采用自身半口对照方法：A组儿童对照侧实验牙应用金刚砂车针行釉质成形术,酸蚀,涂布窝沟封闭剂,实验侧实验牙应用Er：YAG激光行釉质成形术,涂布窝沟封闭剂;B组儿童对照侧实验牙处理同A组,实验牙应用Er：YAG激光行釉质成形术,酸蚀,涂布窝沟封闭剂。术后让受试儿童对治疗评分,定期随访,观察封闭剂保留情况。结果：63．3％的受试儿童愿意主动选择激光治疗。3、6个月复诊时,两组封闭剂的总保留率没有统计学差异（P＞0．05）;12、18个月复诊时,封闭剂材料总保留率：激光＋酸蚀剂组＞传统车针组＞激光组,差异有统计学意义（P＜0．05）。结论：激光是更容易被儿童接受的治疗方式,Er：YAG激光应用于恒牙釉质窝沟封闭时需要配合使用酸蚀剂,增强封闭剂固位。     

Properties of eight methacrylated beta-cyclodextrin composite formulations.

OBJECTIVES: Methacrylated beta-cyclodextrins (MCDs) are novel candidate dental monomers if all or some of the hydroxyl groups of beta-cyclodextrin are substituted with methacrylate groups. The main objective of this study was to evaluate mechanical properties of a number of composite formulations having MCDs as novel dental comonomers. The properties determined were flexural strength (FS), volumetric shrinkage (VS), and degree of conversion (DC). METHODS: A mass fraction of 50% of MCD monomers was mixed with a mass fraction of 50% each of a series of dimethacrylate or monomethacrylate diluent comonomers to produce consistent formulations of a workable viscosity. For comparison a resin mixture of a mass fraction of 50% Bis-GMA and a mass fraction of 50% triethyleneglycol dimethacrylate (a typical dental resin mixture) was also studied. The mixtures were activated with camphorquinone and ethyl 4-dimethylamino benzoate. One part by mass of each activated resin formulation was mixed with three parts by mass of glass filler. Samples for the FS tests were prepared in (2 x 2 x 25) mm3 molds by light-curing the composites for 2 min on each side. The cured samples were immersed in 37 degrees C water for 24 h, and FS was measured with an Instron machine at a crosshead speed of 0.5 mm/min. VS was measured by a computer-controlled mercury dilatometer. DC was measured by near-infrared spectroscopy. RESULTS: The properties of the MCD-based composites depended on the kind of diluent used. With these MCD monomers, diluents of triethyleneglycol dimethacrylate, 1,10-decamethylenediol dimethacrylate, or benzyl methacrylate yielded the best composite properties. SIGNIFICANCE: Although not yet fully optimized, MCD-based composite formulations containing triethyleneglycol dimethacrylate, 1,10-decamethylenediol dimethacrylate, or benzyl methacrylate yielded flexural strength and volumetric shrinkage values were comparable to those of the Bis-GMA/triethyleneglycol dimethacrylate controls. These findings lend support for further development and evaluations of polymerizable cyclodextrin derivatives for use in dental materials.     

ART restorations and glass ionomer sealants in Zimbabwe: survival after 3 years

Atraumatic restorative treatment (ART) consists of removing demineralised tooth tissues with hand instruments only, restoring the prepared cavity and sealing the adjacent pits and fissures with an adhesive filling material. This relatively painless, no-handpiece, minimal intervention approach to controlling dental caries is described. ART was applied in an oral health care programme in Zimbabwe that was carried out amongst secondary school students from 1994 to 1997. A new glass ionomer (Fuji IX) was used as the restorative and sealant material. Sealants were placed in high caries risk students using the ‘press-finger’ technique. A total of 297 one-surface ART restorations and 95 glass ionomer sealants were placed in 142 and 66 students, respectively. After 3 years, the lost-to-follow-up percentages for one-surface ART restorations and glass ionomer sealants were 30.6% and 30.5%, respectively. Actuarial (life table) analysis resulted in 3-year survival rates of one-surface ART restorations of 88.3% (95% CI: 92.4%–84.2%), ranging from 94.3% to 65.4% per operator. A total of 28 ART restorations placed in 25 students failed. Reasons for failure related to the material and the operator (11 restorations or 5.3% each), and to caries adjacent to the restoration (one restoration or 0.5%). Reasons for failure were not recorded for five restorations (2.3%). Seal-ants were placed on surfaces diagnosed as early enamel lesions. After 3 years, 71.4% (95% CI: 81.7%–61.1%) of the fully and partially retained sealants survived with a range of 100% to 55.6% per operator. Of the sealed surfaces 96.3% (95% CI: 100%–92.2%) survived 3 years without developing caries. Experienced operators placed better ART restorations than inexperienced operators. This study has demonstrated that ART with a glass ionomer restorative material and sealants provided high quality preventive and restorative dental care to this student population. ART has become one of the treatment modalities available to oral health workers in managing dental caries.     

不同自酸蚀黏接系统对窝沟封闭剂微渗漏影响的实验研究

目的探讨不同自酸蚀黏接系统对窝沟封闭剂封闭性能和渗透性的影响。方法收集新鲜拔除的离体第三磨牙36颗,随机分成4组,每组9颗,面釉质以杯刷清洁30s;以37%正磷酸酸蚀作为对照组,以ClearFil SE Bond、XenoⅢ以及iBond分别对牙釉质进行黏接作为实验组。涂布可见光固化窝沟封闭剂3MConcise White,光照固化。每组中8颗用1%亚甲基蓝进行染色法检查微渗漏,1颗采用扫描电镜观察封闭剂渗入窝沟的深度以及窝沟封闭剂和牙体组织之间的结合界面的状况。结果Clear FilSE Bond以及XenoⅢ自酸蚀黏接时,对封闭剂微渗漏的影响与对照组相比差异无统计学意义;扫描电镜下,磷酸酸蚀、ClearFil SE Bond以及XenoⅢ组在窝沟封闭中使封闭剂与牙釉质密合性好。结论第五代自酸蚀黏接剂ClearFil SE Bond以及第六代XenoⅢ与牙釉质之间具有良好的封闭能力,可以推荐临床使用。     

3种窝沟封闭剂微渗漏的实验研究

目的:比较3种窝沟封闭剂的微渗漏情况,为选择操作技术难度低且具良好边缘密合性的窝沟封闭剂提供实验依据.方法:收集正畸减数的上颌前磨牙40颗,随机分为5组,每组8颗.即:Helioseal F组、GC Fuji Ⅶ组、GC Fuji Ⅶ+唾液污染组、Embrace WetBond组、Embrace WetBond+唾液污...     

四种因素对窝沟封闭剂渗透性影响的体外研究

目的：体外比较不同窝沟处理方法,窝沟是否污染,不同封闭材料,不同光照时间对窝沟封闭剂渗透性的影响。方法：48颗离体前磨牙,随机分为16组,每组3颗牙,分别采用不同封闭材料及方法进行窝沟封闭,体视显微镜下观察,对窝沟封闭剂的渗透率进行测定。结果：窝沟封闭材料、窝沟处理方法两种处理因素自身不同水平比较,差异具有统计学意义（P〈0.05）,窝沟封闭材料和窝沟处理方法之间存在交互作用（P〈0.05）。结论：窝沟釉质成形术与封闭剂Helioseal F联合应用进行窝沟封闭具有很好的临床应用价值。     

Factors influencing the effectiveness of sealants – a meta-analysis

Abstract – Meta-analysis of published studies was applied to analyze the effectiveness of fissure sealants in preventing dental caries. Studies were identified by a MEDLINE search, supplemented by a hand search of the references in the articles recovered. All articles were assessed on the basis of quality, and were combined with the Mantel-Haenszel method. The prevented fraction in the exposed population (PF) was estimated using pooled relative risk. Heterogeneity of the effect and publication bias were also analyzed. Twenty-four studies were included in the meta-analysis. The overall effectiveness of autopolymerized fissure sealants was 71.36% (95% confidence interval 69.69-72.94%). Effectiveness in preventing caries decreased with time, and increased when drinking water was fluoridated (82.69% vs 72.28%). Heterogeneity was significant in most cases, and was further studied with multiple linear regression analysis. It is concluded that autopolymerizing sealants should be used. More research is needed to compare the effectiveness of dental hygienists and dentists in applying sealants.