Detection of plasmodium falciparum lactate dehydrogenase. A promising approach to quick diagnosis of malaria.

To establish a quick and applicable diagnostic method has been a major target in immunological research on malaria. Of all approaches studied, circulating antigen (CAg) detection seems to be the most promising. Scientists have encountered tough difficulties in the analysis and purification of malaria parasite antigen because of its high complexity. Studies showed that plasmadium falciparum (P.f) lactate dehydrogenase (pLDH), as a specific CAg of malaria parasite. is apparently different from that of human erythrocytes (rLDH) both in their physical and biochemical characteristics. and is very easy to be identifed. Consequently, pLDH detection has a great potential to be developed into a method for assessing parasitemia. This paper reviewed the methods for the purification. separation, identification of pLDH and the prospect of its clinical applications as an ideal detector of the presence of malaria parasite in order to speed up this research.     

Homotransplantation of testis. A report of 11 cases.

Testis homotransplantation has been carried out in 11 patients since 1986. Of them, 8 suffered from congenital testicular dysplasia and the other 3 from orchiatrophy as a result of orchitis. Preoperatively, manifestations were a small penis, small testes, sexual dysfunction, indistinct secondary sex characteristics and low serum testosterone (1.7-7.35 mmol/L). Three cases had cadaver transplants and 8 had living relatives' transplants. The patients were followed up for 7 months to 4 years. The secondary sex characteristics and sexual function were improved remarkably after transplantation. Serum testosterone increased up to 12.8-18.2 mmol/L. All of 5 married patients resumed sexual intercourse satisfactorily. Three others got married after transplantation and have enjoyed normal sexual functions. Spermatozoa have been found in the semen in 3 of the cases.

     

Biliary papillomatosis: analysis of 18 cases

Biliary papillomatosis （BP） is an extremely rare pathological condition, which is characterized by papillary proliferation of the bile duct epithelia. Although initially thought to be a benign entity, this tumor has been shown to have a tendency for malignant transformation. As reported in the literature, malignant degeneration occurs in 20% to 35% of cases. Because patients typically present with abdominal pain, obstructive jaundice and fever, they are usually mistaken as having more serious pathology such as cholangiocarcinoma, cholangitis and so forth. In general, the rate of preoperative diagnosis is very low. At present, the number of published articles about this illness is limited, mainly as single case reports. In addition, the diagnosis, treatment and prognosis of BP have not been well known. We, therefore, discuss age, gender, clinical manifestations, diagnostic approaches, histopathological findings, treatments and prognosis by reviewing the clinical data of eighteen patients with BP treated in our hospital, so as to better understand this rare illness.     

Expression of p30, the major surface antigen of Toxoplasma gondii, in baculovirus-insect cell system and the evaluation of immune response induced by p30

T~ gondii is an intiacellular ProtOZOan Par- optima gondii have been idelldried. One Of the s~easite and widely knoWn tO be infective in hUman, agricul- antigens, P30, has been isolated from the taChyAnte andthai and domestic ~s. It was estimated that as many idennded to have potential use as subedt yao.ine[3]. TIsas one ed Of the world population may have serological PIDtein is also PI'eSent in most of T~. p30 isevidence of infectionlll. Because of the POSSilile long in- highly anti…     

Chemoprevention of gastric cancer: current status

The development of gastric cancer is a multi-factor process.In addition to genetic factors,environmental factors including smoking,low gastric acidity,excessive intake of salt or salty food and low consumption of fresh fruits and vegetables all contribute to the development of gastic cancer.Of particular interest,epidemiological and experimental studies have demonstrated that Helicobacter pylori(H.pylori)infection is causally linked to gastric cancer.Most studies using micronutrient supplementation have failed to demonstrate any preventive effect against the development of gastric cancer.The use of non-steroidal antiinflammatory drugs has been consistently observed to protect against the development of gastric cancer. Recently,eradication of H.pylori infection by a chemopreventative approach is being studied in a number of trials.Studies using precancerous lesions as an end point of the treatment have produced conflicting and mostly negative results.Trials using cancer as an end point are being cautiously carried out in high-risk populations,and will provide the definitive answer to this important question.In the end,vaccination may be proven to be the optimal strategy in human for the management of H.pylori infection and prevention of gastric cancer.     

Experimental study on human umbilical cord blood scheduled transplanta-tion in severe combined immunodeficient mice

UInbilical cold blood (CB), an alternative somee ofhUInan helnatopoietic stelnlP~hc cells (HSClllPC),has been c~ under heated discussion in helhatology.~s studies, in vitro and Paxtly ba aide, have shownthat HSC/gr of CB had a high potentiality Of Pndiferation and mplantation as compel with those of adult acemarrow (BM) and mobilized periphery nlooa (re). Simultaneously, ace have also explode some soluhons,such as hew umbilical cold blood ~fan(HCar) ho some ~ated donors or expansion of…     

The application of nano biomaterials in diabetic wounds

The number of diabetes mellitus, especially type 2 diabetes patients is increasing due to fast population growth, aging, urbanization and increasing prevalence of obesity, as well as lack of physical activity. The delayed wound healing even non-healing is one of the diabetes complications, representing a huge burden for patients and health services. Many new medical technologies have been utilized in this field in recent years. A systematic evaluation of nano biomaterials in diabetic wounds will be summarized and reviewed in this article.     

Study on the Regulation of Bcl-2 Gene on Rat Spermatogenic Cells Apoptosis in Transcription Level

Apoptosis is regarded as a natural phenomenon accompanying the whole periodof spermatogenesis.Therefore,the number of mature sperm was only5 0 % to75 %of the estimated number.Some interventions,such as vasoligation,could promoteapoptosis.Bcl-2 gene,known as an anti-apoptosis gene,plays a very importantrolein spermatogenic cells apoptosis in rats with vasoligation and vasostomy[1 ] ,but thestudies on this key gene have been almostlimited atprotein level so far[2～ 4] .We de-signed this experime…     

Prevention and Treatment of Pneumoconiosis in China

Silicosis is one of the most serious occupational iseases which affect many workers‘ health in China.Under the guidance of policies from the Chinese Government,the industries,health care instituteions,scientists and workers were organized and have made great efforts in prevention and treatment of sillicosis as well as pneumoconissis.Since the 1960s,the national health standards and chest X-ray diagnosis criteria for silicosis were established and have been revised recently.A nationwide epidemiological survey revealed that the cumulative pneumoconiosis cases were about 4800000(1949-1992).The high prevalence rate has been the motivation to search for anti-silicotic drugs.Several kinds of anti-silicotic drugs were studied and found to be able to retard progression of silicosis and cause changes in X-ray picture.The mechanism of drug efficiency and the development of silicosis have attracted the interest of scientists in this fields and fruitful results have been obtained.For prevention of dust havards,the systematic design for facilities of dust collection and the isolation of dust source appropriate to township industries have been worked out and used widely.Recently,under the supervision of the Ministry of Public Health,China,a pilot study on countermeasure for industrial hazards and health care service in township industries has been instituted.     

The Research of Artificial Cervical Disc Replacement

Cervical arthroplasty after anterior decompression with insertion of a prosthetic total disc replacement has been suggested as an alternate to anterior cervical fusion. It develops quickly during recent years. Currently there are several cervical arthroplasty devices. Each device varies in terms of materials, range of motion and constraint. Early studies suggest that in the short term, the complication rate and efficacy is no worse than fusion surgery. Long-term results have not yet been reported. This review examines the current prostheses as well as discussing issues regarding indications and technique. It is hoped that an improvement of cervical arthroplasty occurs in terms of materials and design as spinal surgeons enter a new times of the management of cervical spine disease.     

Evaluation of MRI in the diagnosis of hepatic tuberculoma

Tuberculosis is common in developing countries but hepatic tuberculosis is rare, especially in the form of hepatic tubereulomas or maeronodular tubereuloma. Most of the eases have been misdiagnosed as hepatic tumours on medical imaging. Many reports have described its magnetic resonance image (MRI) and differential diagnosis. This paper is a summary of MRI findings and analysis of 10 cases with hepatic tubereuloma.     

Tripterygium Wilfordii： Structure, Chemistry and Activity of Male Antifertility Constituents

Extracts of the twining vine Lei Gung Teng (Tripterygium wilfodii Hook F.) have been used in traditional Chinese medicine for many centuries for the treatment of inflammatory conditions and skin disorders . The powdered roots of the vine were also used in China as a natural insecticide     

Hepatocellular Carcinoma： the Curative Attempts

Objective The curative result or long-term survival of hepatocellular carcinoma （HCC） has been pursued ever since its discovery even most of the cases are evaluated as absolute-non-cure before their management. A number of treatment approaches have been applied and option for a certain case is absolutely necessary. The objective of this article is to evaluate today＇s treatment methods and find some information for the option. Methods The characteristics of most of these approaches are studied and compared for their role in the curative management. The key points, advantages and disadvantages of these approaches are discussed. Results Current treatments fall into categories of surgical, percutaneous, chemical and physical as well as biomedical ones Different modes of regional cancer therapy for HCC have been tried, but the relative efficacy remains unclear. Anti-angiogenic agents, gene therapy and tumor vaccine will probably play a role, particularly in the prevention of tumor recurrence. Some issues remain to be solved. Conclusion For the recent future, the ideal strategy for curative results might be the establishment of a comprehensive way of combined approaches.     

Pharmacological effects and active phytoconstituents of Swietenia mahagoni: a review

The usage of Swietenia mahagoni, a popular medicinal plant in India and some African countries, dates back to ancient times for its curative properties in diseases like malaria, diabetes, and diarrhea. It is also used as an anti-pyretic, bitter tonic and astringent. Its pharmacological activities are being widely explored. Although many important groups of phytochemicals have been identified and isolated from various parts of the plant, most of these researches have been focused on seeds. Toxicological studies have established the safety of many of these plant extracts, and found insignificant side effects. Here we present a comprehensive review of all the pharmacological effects and constituent phytochemicals of the plant.     

Analysis of nuclear DNA content of cells from liver cell dysplasia and hepatocellular carcinoma

Nuclear DNA content of malignant cells was measured by OPTON III microspectrophotometry in 19 cases of hepatocellular carcinoma (HCC), as well as cells from the paraneoplastic liver tissue. The mean DNA value increased greatly from non-liver cells dysplasia (LCD), LCD to HCC (P<0. 05—0. 01), In histograms, non-LCD hepatocytes showed a single peak type located at 2C. HCC showed lower multiple peaks which migrated to 4C and spread widely with more aneuploid (>5C) cells, This histograms of LCD were between non-LCD and HCC,and there were also some aneuploid cells in LCD. These results indicate that LCD is a group of proliferating cells and those with relatively high ratio of aneuploid cells might be premalignant. Recently, many reports on analysis of nuclear DNA content of esophageal, gastric, nasopharyngeal and lung cancer, as well as cells of precancerous lesions have been published. Most results showed that nuclear DNA content of the conditions above mentioned and their number of chromsome were often alterated. Analysis on nuclear DNA content of hepatocellular carcinoma (HCC) and liver cell dysplasia (LCD) are vary rare. And so far, there have been various considerations on behavor of LCD and its relationship to HCC. We would attempt to further approach biologically nature of LCD and its association with HCC by measuring nuclear DNA content.     

Improving long-term outcomes of coronary intervention in patients with diabetes

In China, the prevalence of type 2 diabetes mellitus is increasing due to the aging of the population, increased frequency of obesity, and suboptimal nutritional habits. The current number of diabetic patients in China is around 40 million. Patients with diabetes even without prior cardiovascular disease have the same event rates as individuals without diabetes but with prior myocardial infarction, Diabetes is present in 25% of patients undergoing percutaneous coronary intervention （PCI）, and a more diffuse and accelerated form of atherosclerosis accompanied by smaller vessel size, longer lesions, and greater plaque burden in diabetic patients may contribute to the well-documented increased risk of neointimal hyperplasia and restenosis after stent implantation in these patients. Clinical as well as angiographic outcomes have been consistently worse for diabetics compared with non-diabetics.     

Immunocytochemical characteristics of cultured human retinal pigment epithelial cells

Rehnal Pigment epithelial cells (RPEC), as one ofthe most ~t cell layers in the eye, po~ a nof fUnctions cmcial in PreServing the integhty Of the visualsystem. The hantenance of healthy RPEC is critical fornOImal retinal funCtion, and the etiologies Of many retinaldisolders may have their Prifnaly origins in the pigment epitheliam. The PIDlilbmtion of RPEC in foe yi~s and onthe surfaCe Of retina is the pathological cause Of Pmlifelative yi~whnOPathy (PVR). On the other hand the a…     

Clinical Application of Lymphaticovenous Anastomosis

Since 1979,16 cases of lymphedema oflimbs have been treated withlymphaticovenous anastomosis in our depart-ment.These patients have been followed upfor 3—9 years.Of 16 patients,14 had lymphedma of lowerlimb and 2 upper limb.One patient was withcongenital lymphedema and 15 secondarycases.The age ranged from 14—56 years.Theduration of illness varied from 2—14 years.