普米克吸入治疗儿童支气管炎哮喘65例临床疗效

目的:分析对儿童支气管炎哮喘行以普米克吸入治疗的临床疗效。方法:择取2016年7月至2018年8月人民医院儿科收治的65例支气管炎哮喘患儿,随机将所选患儿分成对照组和研究组,对照组32例患儿行以可必特吸入治疗,研究组33例患儿在对照组治疗基础上加行普米克吸入治疗,对两组临床疗效进行分析和对比。结果:治疗后,研究组临床总有效率为96.97%,对照组临床总有效率为68.75%,研究组临床总有效率明显比对照组高(P 0.05)。结论:对儿童支气管炎哮喘行以普米克吸入治疗的临床疗效显著,值得推广及应用。     

脾氨肽联合普米克令舒对小儿支气管哮喘疗效及对血清CD4+、CD8+、IgE水平的效果分析

目的:探究脾氨肽联合普米克令舒对小儿支气管哮喘疗效及对血清CD4+、CD8+、IgE水平的影响.方法:选取我院2015年7月到2016年12月间收治的支气管哮喘急性发作患儿76例,随机分为对照组和观察组,各38例,对照组患儿给予普米克令舒,在对照组用药基础上观察组患儿加用脾氨肽.比较两组患儿的临床疗效,CD4+、CD8+及血清IgE浓度.结果:观察组的治疗总有效率高于对照组(P<0.05);观察组CD4+及CD 8+水平均显著高于对照组(P<0.05),且血清IgE浓度低于对照组(P<0.05).结论:脾氨肽联合普米克令舒治疗小儿支气管哮喘疗效更高,可迅速改善患儿免疫功能,值得在临床推广.     

普米克令舒治疗儿童咳嗽变导型哮喘的临床观察

目的观察普米克令舒治疗儿童咳嗽变异型哮喘的临床效果。方法门诊诊治的68例咳嗽变异型哮喘患儿随机分为两组。治疗组34例,予普米克令舒雾化吸入,对照组34例,予必可酮雾化吸入,两组均合用酮替芬及舒喘灵。观察治疗后咳嗽改善情况,作统计学分析。结果治疗组与对照组差异有显著性,两组有统计学意义(p<0.05)。结论普米克令舒联合抗过敏药及支气管扩张剂能有效治疗儿童咳嗽变异型哮喘,应用更方便。     

普米克气雾剂治疗小儿咳嗽变异性哮喘87例疗效观察

目的探讨普米克气雾剂治疗小儿咳嗽变异性哮喘的临床效果。方法选择笔者所在医院2008年2月～2010年11月咳嗽变异性哮喘患儿174例，两组患儿随机分为观察组和对照组。两组均为87例。对照组采用常规治疗：给予抗过敏药、支气管扩张药、镇咳等药物，合并感染患儿给予抗生素。观察组患儿在常规治疗基础上给予普米克气雾剂吸人治疗。结果观察组总有效率与对照组比较，差异有统计学意义（P〈0．05）。结论普米克气雾剂吸人治疗能够显著改善咳嗽变异性哮喘患儿临床症状，临床效果显著，值得借鉴。     

雾化吸入博利康尼及普米克令舒治疗支气管哮喘急性发作疗效观察

目的:探讨雾化吸入博利康尼及普米克利舒治疗支气管哮喘急性发作的效果.方法:选择在我院接受治疗的80例支气管哮喘患者,随机分为治疗组和对照组,对照组采用传统治疗方法,控制感染,茶碱类、静滴肾上腺皮质激素、氧疗及维持水电解质、酸碱平衡等治疗,治疗组在此基础上加用博利康尼、普米克令舒雾化吸入,疗程7天.结果:治疗组总有效率95%,对照组总有效率70%,治疗组高于对照组.结论:雾化吸入博利康尼及普米克令舒治疗支气管哮喘急性发作安全有效,值得临床推广.     

普米克令舒联合爱全乐雾化吸入治疗支气管哮喘急性发作的临床疗效

目的:研究普米克令舒联合爱全乐雾化吸入治疗支气管哮喘急性发作的临床疗效.方法:2015年2月至2016年2月期间,选择本院收治的支气管哮喘急性发作患儿108例进行研究分析,随机将患儿分为2组,其中对照组共54例,采用抗感染、化痰、吸氧等常规治疗,观察组患儿共54例,在常规治疗的基础上增加普米克令舒联合爱全乐雾化吸入治疗,对两组患儿的临床疗效进行比较分析.结果:治疗后,观察组患儿显效率与有效率之和为94.44％,对照组患儿显效率与有效率之和为77.78％,观察组患儿治疗效果显著优于对照组,数据比较差异具有统计学意义(P＜0.05).结论:对支气管哮喘急性发作患儿采用普米克令舒联合爱全乐雾化吸入治疗能够明显缓解患儿的临床症状,治疗效果显著,值得推广应用.     

普米克气雾剂治疗小儿变异性哮喘

目的观察普米克气雾剂治疗小儿变异性哮喘的临床效果。方法选择60例变异性哮喘患儿随机分为对照组和观察组,对照组给予抗过敏、扩张支气管、止咳等药物治疗,观察组在对照组治疗的基础上加用普米克气雾剂治疗。结果对照组总有效率63.3%,观察组总有效率90.0%,两组比较差异有统计学意义(P<0.05);观察组复发率16.6%,对照组复发率43.3%,两组比较差异有统计学意义(P<0.05)。结论普米克气雾剂治疗小儿变异性哮喘疗效好、复发率低、不良反应小,可在临床推广应用。     

普米克令舒混悬液雾化吸入治疗婴幼儿哮喘疗效观察

目的:观察普米克令舒雾化混悬液雾化吸入治疗婴幼儿哮喘的临床疗效。方法:选择132例婴幼儿哮喘患儿随机分为治疗组和对照组。治疗组70例,加用普米克令舒混悬液通过空气压缩泵雾化吸入治疗;对照组62例,不用普米克令舒雾化混悬液雾化吸入治疗。余治疗措施两组均相同。结果:治疗组有效68例,总有效率97.14%;对照组有效46例,总有效率74.6%。两组比较有显著差异(P<0.01)。结论:普米克令舒雾化混悬液雾化吸入治疗婴幼儿哮喘疗效显著,副作用小,值得临床推广使用。     

普米克令舒雾化治疗孕妇急性支气管哮喘的疗效观察

目的:探讨普米克令舒雾化治疗孕妇急性支气管哮喘的临床疗效。方法:将近3年呼吸科收治的60例孕妇急性支气管哮喘患者,随机分为观察组和对照组各30例,均采用常规治疗(抗感染、茶碱类等治疗)。观察组在此基础上,采用墙壁高流量氧气雾化吸入普米克令舒4 mg,2次/d。观察组治疗7天后,按《支气管哮喘防治指南》判定疗效。结果:观察组临床控制15例,显效9例,好转3例,总有效率为90%。对照组临床控制2例,显效3例,好转12例,总有效率为42%。两组疗效差异有统计学意义(P<0.05)。结论:普米克令舒雾化吸入治疗孕妇急性支气管哮喘疗效可靠,无全身不良反应,值得临床推广。     

支气管哮喘患儿血清炎性因子水平与氧化应激状态评估

目的探讨支气管哮喘患儿血清氧化应激状态与炎性因子水平及相互关系。方法选择支气管哮喘患儿97例作为观察组,其中发作期及缓解期分别有53例、44例。对照组选择健康儿童50例,分别检测两组儿童MCP-1、MDC、Hs-CRP及MDA、SOD、GSH。结果观察组缓解期患儿MCP-1、MDC、Hs-CRP较发作期均有显著性下降(P0.05),对照组MCP-1、MDC、Hs-CRP较发作期及缓解期患儿均有显著性下降(P0.05)。观察组缓解期患儿SOD及GSH较发作期均有显著性升高(P0.05),MDA显著下降(P0.05)。对照组SOD及GSH较发作期及缓解期均有显著性升高(P0.05),MDA较发作期有显著性下降(P0.05)。MCP-1与MDA、SOD、GSH呈显著相关(P0.05),MDC与SOD、GSH呈显著负相关(P0.05),Hs-CRP与MDA、SOD、GSH未见显著相关性(P0.05)。结论支气管哮喘患儿炎性因子水平失衡与氧化应激状态密切相关,是参与支气管哮喘发病过程的重要因素。     

普米克令舒和万托林雾化吸入治疗毛细支气管炎疗效观察

目的：探讨普米克令舒和万托林雾化吸入治疗小儿毛细支气管炎（毛支）的疗效。方法：将42例毛支患儿随机分为两组,两组均采用综合治疗,观察纽加用普米克令舒和万托林雾化吸入,临床观察气急缓解时间、喘鸣音消失时间及总有效率。结果：观察组在治愈率、缓解喘憋、缩短哮鸣音及咳嗽持续时间、改善肺功能、均明显优于对照组（P〈0．05）。结论：普米克令舒和万托林雾化吸入治疗小儿毛支,可缩短病程,改善肺功能,且方便、安全,可作为治疗毛支的主要药物。     

An open-label dose-response study of lymphocyte glutathione levels in healthy men and women receiving pressurized whey protein isolate supplements

BACKGROUND: High-pressure treatment of whey protein may increase digestibility and bioavailability of cysteine. The purpose of the study was to determine whether total lymphocyte glutathione (gamma-glutamyl-cysteinyl-glycine [GSH]) levels (oxidized+reduced) can be augmented from three different doses of pressurized whey protein supplements in a dose-dependent manner over a 2-week period. METHODS: Eighteen healthy males and 18 healthy females were randomized into three different groups, with 31 finishing the study. Each group ingested 15, 30, or 45 g/day pressurized whey protein in the morning in bar format for 14 days. Each group was blinded to the amount of whey protein they were ingesting. Ten millilitres of blood was withdrawn before and after the 2-week period to assess blood lymphocyte levels pre and post supplementation. RESULTS: There was no change in body weight or reported physical activity levels pre and post supplementation. Pre-lymphocyte GSH levels were not significantly different between groups (3.7+/-0.7 micromol/l). Least-squares linear regression showed that the change in lymphocyte GSH levels from pre to post supplementation was affected by the amount of whey protein ingested daily (P=0.037). The group that ingested 45 g/day pressurized whey protein augmented GSH levels the most (by approximately 24%), and the group that ingested 15 g/day did not increase lymphocyte GSH levels. CONCLUSIONS: We conclude that there is a significant relationship between the dosage of supplementation and the change in lymphocyte GSH levels. Furthermore, the increase in GSH was linear with the amount of whey protein ingested. Pressurized whey protein supplementation of 45 g/day for 2 weeks can increase lymphocyte GSH by 24%.     

Selenium glycinate supplementation increases blood glutathione peroxidase activities and decreases prostate-specific antigen readings in middle-aged US men

Two concepts are often currently applied to selenium in adult men in the United States:

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Intake is generally enough to maximize blood glutathione peroxidase activities.

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In such men, selenium supplementation does not reduce risk of prostate cancer.

In contrast to these concepts, 30 healthy middle-aged men were studied to test the following hypothesis: 6-week supplementation of 200 μg of selenium as glycinate can raise activities of 2 blood selenium enzymes and lower a marker of prostate cancer risk. The hypothesis was confirmed, in that selenium supplementation raised activities for erythrocyte and plasma glutathione peroxidase as well as lowered values for plasma prostate-specific antigen. The enzyme activity increases were not extremely large, but based on a chicken study, changes in blood glutathione peroxidase activities can reflect bigger changes in the prostate. Placebo treatment did not duplicate the selenium effects in 30 other men. In conclusion, this study suggests that US middle-aged men may not typically consume optimal amounts of selenium.     

Plasma selenium and plasma and erythrocyte glutathione peroxidase activity increase with estrogen during the menstrual cycle

OBJECTIVE: This study was designed to determine the timing and magnitude of changes in selenium status in relation to the fluctuation of 17-beta-estradiol during the menstrual cycle and the effect of different phases of the menstrual cycle on dietary selenium intake. METHODS: Plasma 17-beta-estradiol and plasma and erythrocyte selenium and glutathione peroxidase (GPx) activity were measured in fasting blood samples collected in the morning at four times over three phases of the menstrual cycle: early follicular (EF: days 1-3 menstruation), periovulatory (PO; E-1: 1 day before estrogen peak and E: during estrogen peak) and mid-luteal (ML: 7-9 days after ovulation) in healthy women (n = 14) aged 21 to 39 years and with regular menstrual cycles (26 to 30 days). The estrogen peak was confirmed by measurement of the luteinizing hormone surge. Dietary records (three-day) coincided with blood collection for each phase. RESULTS: Plasma selenium and plasma and erythrocyte GPx activity were greatest during the periovulatory phase, coinciding with the estrogen peak. No differences were observed for erythrocyte selenium or dietary selenium throughout the cycle. A linear relationship existed between estradiol and plasma selenium (p < 0.0027), plasma GPx activity (p < 0.0001), and erythrocyte GPx activity (p < 0.0001). CONCLUSIONS: These results indicate that blood selenium parameters fluctuate during the menstrual cycle such that the phase of the cycle should be considered when assessing selenium status.     

Metabolism of selenite and selenomethionine in the rhesus monkey

Female rhesus monkeys were fed a commercial monkey diet and given selenium (Se) as either selenite or selenomethionine (SeMet) in the drinking water for 11 mo. Muscle and liver biopsies were taken initially and at the end of the experiment for determination of Se levels and glutathione peroxidase (GPX) activity. Blood was collected at monthly to bimonthly intervals, and the plasma and erythrocytes were subjected to gel filtration to determine the distribution of Se among proteins of various molecular weights. At the end of the experiment, there was significantly more Se in liver, muscle and hair from the monkeys given SeMet than in tissues from those given selenite, but there were no differences in liver or muscle GPX activity between the two treatment groups. The erythrocyte and plasma Se levels were significantly higher in the monkeys given SeMet than in those receiving selenite, but there were no differences in the GPX levels between these groups. About 68% of erythrocyte Se was associated with GPX in monkeys given selenite whereas only 34% was associated with GPX in those administered SeMet. The correlation coefficient for blood Se level and erythrocyte GPX activity was 0.92 in monkeys given selenite but only 0.37 in those given SeMet. Gel filtration of plasma revealed only one Se peak for plasma from the monkeys given selenite but at least two major Se peaks for plasma from monkeys receiving SeMet. The possible implications of these results for humans are discussed, including the reasons for poor correlations of GPX activity and blood Se levels.     

Effect of different hydrolysates of whey protein on hepatic glutathione content in mice

This study was designed to compare the effects of diets prepared with enzymatic hydrolysate of a whey protein concentrate (WPC) by pancreatin, protamex (Novo Nordisk, Bagsvaerd, Denmark), and alcalase proteases on the hepatic glutathione content in mice. The undenatured WPC was produced in a pilot plant by membrane technology (microfiltration/diafiltration) after separation of the casein clot through a conventional process. All three hydrolysates with 20% degree of hydrolysis showed an amino acid profile similar to WPC. Male A/J mice were fed on diets containing 20% WPC or hydrolysates. Commercial casein was used as a reference protein in the biological assays. The glutathione content was determined after liver extraction through high-performance capillary electrophoresis. WPC and its pancreatin and protamex hydrolysates showed higher ability to stimulate liver glutathione synthesis than alcalase hydrolysate. This difference was probably related to an amino acid sequence in the peptides that were formed during hydrolysis of whey proteins. Commercial casein and WPC alcalase hydrolysate produced lower stimulation of liver glutathione synthesis (7.09 and 5.66 micromol/g of wet weight) compared with WPC and pancreatin and protamex hydrolysates (8.72, 8.71, and 8.45 micromol/g of wet weight, respectively). These results indicate that the hydrolysates obtained by treatment with pancreatin and protamex are good sources of peptides with activity to stimulate glutathione synthesis.     

Selenium and vitamin E status of healthy and institutionalized elderly subjects: analysis of plasma, erythrocytes and platelets

Levels of selenium in whole blood, plasma, erythrocytes and platelets, glutathione peroxidase (EC 1.11.1.9; GSH-Px) activity in erythrocytes and platelets and vitamin E, low-density-lipoprotein (LDL)-cholesterol and vitamin E: LDL cholesterol in plasma were measured in seventy-five healthy subjects aged less than 65 years and twenty-eight healthy and twenty-three institutionalized elderly people aged greater than 65 years. Healthy elderly subjects had significantly lower levels of Se in whole blood and plasma when compared with younger subjects. Other measurements of Se status were not significantly different. In the healthy subjects plasma levels of vitamin E and LDL-cholesterol increased with age to 60 years and decreased above 80 years. Vitamin E: LDL cholesterol values were not affected by age. Measurements of Se and vitamin E status in the institutionalized elderly compared with the healthy elderly were all reduced with the exception of platelet Se levels and erythrocyte GSH-Px activity. Ageing per se had minimal effect on Se and vitamin E status but intercurrent illness and decreased food intake can lead to reduced levels in the elderly.     

Relationship between blood antioxidants and occupational exposure to polycyclic aromatic hydrocarbons in coke oven workers

Background We investigated if blood Cu⁺⁺/Zn⁺⁺ superoxide dismutase, glutathione peroxidase, and catalase activities are increased and total plasma antioxidant concentration is decreased in coke oven workers exposed to polycyclic aromatic hydrocarbons. Methods Ninety-six coke oven workers participated in the study. Nonexposed workers (n = 105) were randomly sampled among power plant workers in the same age range. The examination included a questionnaire on health status, occupational history, smoking, and dietary habits. Blood samples completed the examination. Coke oven workers were classified into low-, middle-, and high-exposure groups based on the benzo[a]pyrene (B[a]P) air concentrations and were further classified into the categories “topside” and “non-topside,” according to their proximity to the ovens. Results Erythrocyte glutathione peroxidase activity increased with age (r = 0.18, P = 0.061) in power plant workers, whereas plasma glutathione peroxidase activity decreased with age (r = −0.18, P = 0.068) and erythrocyte glutathione peroxidase activity was inversely correlated with the number of cigarettes per day (r = −0.28, P = 0.08) in coke oven workers. Comparison of blood antioxidant enzyme activities and total plasma antioxidant concentration between coke oven and power plant workers showed that erythrocyte glutathione peroxidase activity was significantly lower in coke oven workers, even after adjustment for potential confounding factors. No differences were found either in other blood antioxidant enzyme activities or in total plasma antioxidant concentration between coke oven and power plant workers. Moreover, no trends toward decreased glutathione peroxidase activity among the three subgroups of B[a]P exposure were observed, and no differences either in blood antioxidant enzyme activities or in total plasma antioxidant concentration between the two groups of job categories were found. Conclusions Production of reactive oxygen species seems not to be increased in coke oven workers. Am. J. Ind. Med. 34:272–279, 1998. © 1998 Wiley-Liss, Inc.     

Plant-Based,Antioxidant-Rich Snacks Elevate Plasma Antioxidant Ability and Alter Gut Bacterial Composition in Older Adults

Plant-rich diets alleviate oxidative stress and gut dysbiosis and are negatively linked to age-associated chronic disorders. This study examined the effects of consuming plant-based, antioxidant-rich smoothies and sesame seed snacks (PBASS) on antioxidant ability and gut microbial composition in older adults. Healthy and sub-healthy older adults (n = 42, 79.7 ± 8.6 years old) in two senior living facilities were given PBASS for 4 months. Blood and fecal samples were collected from these individuals at the baseline and after 2 and 4 months of PBASS consumption. After 2 months, serum levels of albumin and high-density lipoprotein-cholesterol and the ratio of reduced to oxidized glutathione (GSH/GSSG) had increased significantly and erythrocytic glutathione, GSH/GSSG and superoxide dismutase activity had decreased significantly compared with baseline levels (p < 0.05). After 4 months, red blood cells, hematocrit, serum blood urea nitrogen and erythrocyte glutathione peroxidase activity had decreased significantly, whereas plasma and erythrocyte protein-bound sulfhydryl groups had increased significantly. Furthermore, plasma glutathione and total antioxidant capacity were significantly greater after 2 months and increased further after 4 months of PBASS consumption. The results of next generation sequencing showed that PBASS consumption prompted significant decreases in observed bacterial species, their richness, and the abundance of Actinobacteria and Patescibacteria and increases in Bacteroidetes in feces. Our results suggest that texture-modified, plant-based snacks are useful nutrition support to benefit healthy ageing via the elevation of antioxidant ability and alteration of gut microbiota.     

Selenium, vitamin E and the response to swimming stress in the rat.

Experiments were conducted to determine the effects of exercise on rat glutathione peroxidase system enzymes and lipid peroxidation among animals supplemented and unsupplemented with selenium (Se) and vitamin E (E). Liver, muscle and blood were taken before, immediately after and 24 hours after exercising to exhaustion by swimming. No effect of exercise was found on muscle or liver enzymes, although exercise resulted in depressed glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) activities in erythrocytes immediately after exercise. Dietary Se supplementation did result in increased hepatic muscle and erythrocyte glutathione peroxidase activity, and decreased hepatic GR, G6PD and "malic enzyme" activities. Thiobarbituric acid reactive substances, and indicator of lipid peroxidation, increased in liver and muscle subsequent to exercise. This increase was reduced in liver, but not eliminated, by dietary E supplementation. The increase was not affected by dietary E in muscle, nor by dietary Se in either tissue.