妊娠晚期妊高征患者血清sVCAM-1和sICAM-1水平检测及临床意义

目的 探讨妊娠晚期妊高征(PIH)患者血清可溶性血管细胞粘附分子-1(soluble vascular cell adhesion molecule-1,sVCAM-1)和可溶性细胞间粘附分子-1(soluble intercelluar adhcsion molecule-1,sICAM-1)检测的意义。方法 用ELISA法检测37例PIH患者及14例正常妊娠妇女血清sVCAM-1和sICAM-1的含量。结果 svcam-1在中重度妊高征组明显高于正常对照组和轻度妊高征组(P<0.05),轻度妊高征组与正常对照组无明显差异。(P>0.05);sICAM-1在各组间无明显差异(P>0.05)。结论 妊娠晚期血清sVCAM-1 水平明显升高,sICAM-1变化不明显。     

冠心病患者细胞粘附分子的水平变化和临床意义

目的：探讨冠心病患者外周血白细胞粘附分子β2整合素(β2-intergrin，CD18)和血清可溶性细胞间粘附分子-1(soluble intercellulal adhesion molecule-1，sICAM-1，CD54)、血管细胞粘附分子-1(soluble vascular cell adhesion molecule-1，sVCAM-1，CD106)变化及其意义。方法：采用流式细胞仪技术检测45例冠心病患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1的表达。结果：冠心病患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1表达显著增加。结论：冠心病患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1的水平对冠心病的早期诊断具有一定的临床意义。     

肝癌患者粘附分子和细胞因子的变化及其意义

目的：观察肝癌患者外周血清可溶性P-选择素(soluble P-selectin，CD62P)，细胞间粘附分子-1(soluble intercellular adhesion molecule-1，sICAM-1，CD54)，血管细胞粘附分子(soluble vascular cell adhesion molecule-1，sVCAM-1，CD106)和白细胞粘附分子β2整合素(β2-integrin，CD18)的变化，并探讨其临床意义。方法：采用流式细胞仪技术检测了48例不同期肝癌患者外周血清sP-selectin，sICAM-1 sVCAM-1水平和白细胞CD18的表达。结果：与正常对照组相比，血清sP-selectin，sICAM-1 sVCAM-1水平和白细胞CD18的表达显著增加。结论：肝癌患者外周血清sP-selectin，sICAM-1 sVCAM11水平和白细胞CD18的表达对肝癌的早期临床分期具有一定的参考价值。     

膀胱癌患者细胞粘附分子水平的变化及其意义

目的：探讨膀胱癌患者外周血白细胞粘附分子β2（整合素（β2－integrin,CD18)和血清可溶性细胞间粘附分子－1（soluble intercellular adhesion molecule-1 sICAM-1,CD54),血管细胞粘附分子（soluble vascular cell adhesion molecule-1,sVCAM-1 CD106)变化及其意义。方法：采用流式细胞技术检测30例膀胱癌患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1的表达，结果：膀胱癌患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1表达显著增加。结论：膀胱癌患者外周血白细胞CD18和血清sICAM-1和sVCAM-1的水平对膀胱癌的早期诊断病理分级、临床分期具有明确的预示作用。     

阿托伐汀对急性冠状动脉综合征患者血清可溶性细胞间黏附分子-1和可溶性血管细胞黏附分子-1的影响--附68例检测结果分析

目的:观察早期应用阿托伐汀对急性冠状动脉综合征患者的可溶性细胞间黏附分子-1(soluble intercellular adhesion molecules-1,sICAM-1)和可溶性血管细胞黏附分子-1(soluble vascularcell adhesion molecules-1,sVCAM-1)的影响,探讨阿托伐汀降低急性冠状动脉综合征炎症反应的可能机制.方法:稳定型心绞痛30例,急性冠状动脉综合征68例,后者再分为两亚组:阿托伐汀组35例,非阿托伐汀组33例.用酶联免疫吸附法测定急性冠状动脉综合征患者入院后第1、3、5、7、14日的sICAM-1和sVCAM-1水平,观察阿托伐汀对其sICAM-1和sVCAM-1水平的影响.结果:急性冠状动脉综合征患者入院时血清sICAM-1和sVCAM-1水平均比稳定型心绞痛组明显增高(P＜0.01).治疗后急性冠状动脉综合征两亚组的血清sICAM-1和sVCAM-1水平均有不同程度的降低,其中阿托伐汀组的下降更显著.结论:阿托伐汀可以降低炎症反应,在急性冠状动脉综合征的早期治疗中起重要作用.     

系统性红斑狼疮患者血清中可溶性细胞间黏附分子-1和可溶性血管内皮细胞黏附分子-1检测分析

系统性红斑狼疮（SLE）是一种以血管炎为病理特征的自身免疫性疾病,其发病机制不清,黏附分子在SLE发病中的作用日益受到重视.细胞黏附分子是一类介导细胞与细胞外基质间黏附作用的膜表面糖蛋白,而细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）属细胞黏附分子免疫球蛋白超家族成员,它们参与调节炎症反应,组织细胞的发育和分化,免疫应答反应,介导白细胞与内皮细胞以及细胞外基质间黏附作用,参与淋巴细胞归巢和再循环及肿瘤的浸润和转移等[1].为探讨循环中可溶性细胞间黏附分子-1（soluble intercellular aelhesion molecule-1,sICAM-1）和可溶性血管内皮细胞黏附分子-1（soluble vascular cell adhesion molecule-1,sVCAM-1）在SLE发病中的意义,我们检测了31例SLE患者血清中sICAM-1和sVCAM-1的水平,报告如下。     

血清sVCAM-1水平与2型糖尿病脑梗死关系探讨

目的:探讨血清中可溶性血管细胞黏附分子-1(sVCAM-1)水平与2型糖尿病(T2DM)患者并发脑梗死的关系探讨。方法:应用ELISA法检测了68例T2DM患者血浆sVCAM-1水平,并且与20例健康人作对照。结果:T2DM各组血清sVCAM-1水平明显高于健康对照组(P<0.01);无脑梗死组、小灶性脑梗死组、大面积脑梗死组含量逐步升高(P<0.01);血清sVCAM-1水平与LDL-C、TG、TC呈正相关(P<0.05)。结论:血清sVCAM-1参与2型糖尿病脑血管并发症的发生与发展,可作为早期2型糖尿病脑血管并发症发生的预测及检测指标。     

乙型肝炎肝硬化患者血清可溶性细胞间黏附分子-1、白细胞介素-18测定及临床意义

目的:探讨乙型肝炎肝硬化患者血清可溶性细胞间黏附分子-1(soluble intercellular adhesion molecule-1,sICAM-1)、白细胞介素(interleukin,IL)-18的变化及其临床意义.方法:采用ELIAS法对60例乙型肝炎肝硬化患者(肝硬化组)和30例健康体检者(对照组)血清sICAM-l、IL-18水平进行检测.结果:肝硬化组血清sICAM-1,IL-18水平高于对照组(P<0.01).肝硬化Child C级组血清siCAM-1,IL-18水平高于Child A级组和B级组,Child B级组高于Child A级组,差异均有统计学意义(P<0.01).肝硬化组血清sICAM-1,IL-18水平与凝血酶原活动度,白蛋白呈负相关,与总胆红素呈正相关,与丙氨酸转氨酶无相关性.血清sICAM-1水平与IL-18呈正相关.结论:sICAM-1,IL-18水平在一定程度上反映肝细胞损伤程度和肝硬化严重程度,可为临床诊治、评判预后提供依据.     

急性脑梗死患者可溶性共刺激分子B7-H3、可溶性细胞间黏附分子-1表达及临床意义

目的 探讨急性脑梗死患者可溶性共刺激分子B7-H3(soluble costimulatory molecule B7-H3,sB7-H3)、可溶性细胞间黏附分子-1(soluble intercellular adhesion molecule-1,sICAM-1)表达及临床意义.方法 选取2019年3月至2021年...     

中药复方制剂通心络对心绞痛患者血清细胞因子影响的研究--附90例检测结果分析

目的:观察中药复方制剂通心络对心绞痛患者血清细胞因子水平的影响,探讨其治疗心绞痛的可能机制.方法:把90例心绞痛患者分为2组,45例在常规治疗的基础上加用通心络治疗为通心络组,余45例只用常规治疗为对照组,分别用ELISA和放射免疫法测定两组治疗前与治疗8周后血清可溶性细胞间黏附分子-1(soluble intercellular adhesion molecules 1,sICAM-1)、可溶性血管细胞黏附分子-1(soluble vascular cellular adhesion molecules 1,sVCAM-1)、肿瘤坏死因子-α(tumor necrosisfactor-α,TNF-α)和转化生长因子-β(transforming growth factor-β,TGF-β)的水平,观察通心络对心绞痛患者血清细胞因子水平的影响及临床症状改善情况.结果:通心络组治疗后血清sICAM-1,sVCAM-1和TNF-α水平较治疗前明显下降(均为P＜0.05),TGF-β水平较治疗前增加(P＜0.05).而对照组治疗前后上述4项指标比较差异均无统计学意义(均为P＞0.05).与对照组治疗后比较,通心络组sICAM-1、sVCAM-1和TNF-α水平降低更明显(均为P＜0.05),TGF-β水平升高更明显(P＜0.05).结论:通心络可抑制动脉粥样硬化的炎症反应,具有良好的心血管保护作用,可明显改善心绞痛患者的症状.     

HMGB1和细胞凋亡在宫颈病变中的表达及意义

[目的]探讨宫颈病变的高迁移率族蛋白B1(HMGB1)表达和细胞凋亡情况.[方法]分别应用免疫组化和末端核酸转移酶脱氧尿嘧啶标记法(TUNEL).检测并比较正常宫颈组织、宫颈上皮内瘤变(CIN)各期、宫颈鳞癌ⅠA期中HMGB1的表达和细胞凋亡情况.[结果]HMGB1在正常宫颈、CINⅠ、CINⅡ、CINⅢ、宫颈鳞癌ⅠA...     

脑实质囊虫病亚临床期的1H-MRS成像研究

目的应用单体素~1H-MRS波谱成像前瞻性研究对其脑实质囊虫病亚临床期的诊断价值。材料与方法筛选110例脑实质囊虫病亚临床期为研究对象,并与130例脑实质囊虫病活虫期为对照,进行单体素~1H-MRS波谱成像检查,观测脑实质囊虫(亚临床期)病灶及其周围区域某些微量代谢产物比值的变化,并进行对照评价。结果脑实质囊虫病亚临床期与正常镜像区组各生化代谢物比值结果均无显著性差异;脑实质囊虫病亚临床期组与活虫期组统计学分析评价,其中NAA/Cho比值、Cho/Cr比值及Lip/Cr比值有差异性统计学意义,NAA/Cho比值高于活虫期组,Cho/Cr比值及Lip/Cr比值均低于活虫期组,并选择NAA/Cho比值及Lip/Cr比值应用ROC曲线分析评价脑实质囊虫病亚临床期与活虫期鉴别诊断效能。结论揭示脑实质囊虫病亚临床期和活虫期病灶~1H-MRS波谱变化特征及其变化规律,制定出相应的NAA/Cho比值及Lip/Cr比值诊断指标,对于早期诊断及早治疗具有非常重要的指导意义。     

1035例乳腺肿物细针吸取细胞学的分析

目的：分析乳腺肿物细针吸取细胞学（FNAC）对乳腺恶性肿瘤诊断的敏感性。方法：复习乳腺肿物FNAC检查1035例,与其中手术后获病理组织学诊断502例做对照比较。结果：乳腺肿物FNAC对乳腺恶性肿瘤诊断的敏感性为98.9％,特异性为85.7％。细胞学诊断为阳性者（IV、V级）,术后除1例（IV级）外,均证实为恶性。结论：FNAC诊断乳腺癌较为敏感。     

T1 mapping评价扩张型心肌病心肌纤维化

目的探讨T1 mapping评价扩张型心肌病(DCM)心肌纤维化的应用价值。方法对32例DCM患者(DCM组)和16名健康体检者(对照组)行T1mapping和延迟钆增强(LGE)扫描。根据DCM患者是否存在LGE分为LGE(+)亚组和LGE(-)亚组。根据LGE形态和区域,将LGE(+)亚组心肌分为线状LGE区、斑片状LGE区、弥漫性LGE区及远程区(正常心肌区)。分别测量对照组、DCM组、线状LGE区、斑片状LGE区、弥漫性LGE区、远程区T1值,并进行统计学分析。结果 DCM组T1值[(1332.55±61.34)ms]明显高于对照组[(1222.52±45.59)ms,P<0.001]。对照组心肌T1值与线状LGE区[(1359.44±77.93)ms]、斑片状LGE区[(1456.49±110.27)ms]、弥漫性LGE区[(1524.17±52.30)ms]及远程区[(1329.11±64.12)ms]比较,差异均有统计学意义(P均<0.001)。LGE(+)亚组T1值[(1341.35±65.01)ms]与LGE(-)亚组[(1310.06±45.57)ms]差异无统计学意义(P=0.199)。LGE和T1mapping成像诊断DCM组心肌纤维化的ROC曲线下面积分别为0.84、0.95。结论 T1mapping评价DCM心肌纤维化具有较高的应用价值。     

冠心病患者血浆大内皮素水平与N末端脑钠肽及心功能关系的研究

目的 观察冠心病（CHD）患者血浆大内皮素（Bigendothelin-1，BigET-1）含量变化并探讨其与N末端脑钠肽（NT-proBNP）及心功能的关系。方法 选择经冠状动脉造影确诊的CHD患者1292例，健康对照者160名，用酶联免疫吸附分析（ELISA）法测定其血浆BigET-1和NT-proBNP水平，并进行对比分析。结果 CHD患者组血浆BigET-1和NT-proBNP水平[（2．82fmol／m1）及（643．70fmol／m1）]显著高于健康对照组[（2．06fmol／ml）及（397．6fmol／ml）]，差异有统计学意义（P〈0．01）；且冠心病心肌梗死（MI）组[（2．90fmol／ml）及（801．55fmol/ml）]〉心绞通（AP）组[（2．77fmol／ml）及（539．95fmol／ml）]〉健康对照组[（2．06fmol／ml）及（397．6fmol／ml）]，P〈0．01。方差分析显示CHD患者组血浆BigET-1和NT-proBNP浓度都随着冠心病的严重程度加重（NYHA心功能分级升高）而显著上升，各组间差异均有统计学意义（P：0．024，P〈0．01）；多因素相关分析表明血浆BigET-1和NT-proBN浓度与美国纽约心脏病协会（NYHA）心功能分级之间具有良好的正相关性（r=0．359，P〈0．01及r=0．602，P〈0．01），BigET-1和NT-proBNP也密切相关（r=0．315，P〈0．01）。结论 BigET-1和NT-proBNP可能参与了冠心病及心功能损伤的病理生理过程，血浆BigET-1升高与NT-proBNP升高呈正相关。     

Secondary conditions in a community-based sample of women with physical disabilities over a 1-year period

OBJECTIVE: To examine prevalence and predictors of secondary conditions in women with physical disabilities. DESIGN: Cross-sectional. SETTING: Women were recruited through private and public health clinics and various community organizations. PARTICIPANTS: A sample of 443 predominantly ethnic minority women with physical disabilities. INTERVENTIONS: Not applicable. MAIN OUTCOME MEASURE: Health Conditions Checklist interference score. RESULTS: Aggregated data over a 1-year period showed that nearly the entire sample reported interference from pain (94.5%) and fatigue (93.7%) and that at least three quarters of the sample reported problems with spasticity (85.4%), weakness (81.8%), sleep problems (80.2%), vision impairment (77.9%), and circulatory problems (77.9%). Obesity was substantially more prevalent in this sample (47.6%) than in the general population of women (34.0%). The mean number of secondary conditions per woman +/- standard deviation was 14.6+/-6.2 (range, 1-42), with 75% of the sample endorsing 10 or more conditions. On average, women reported experiencing 5.7+/-4.03 (range, 0-20) conditions that they rated as significant or chronic. A third (33.4%) of the variance in interference scores was accounted for in the regression analysis, with significant variance accounted for by race, disability type (women with joint and connective tissue disorders and women with postpolio reported the highest overall interference scores), greater functional limitations, and lower levels of general mental health. CONCLUSIONS: Secondary conditions in women with physical disabilities are substantially more problematic than reported previously in the literature. Further research is needed to determine health disparities of women with and without disabilities. Measurement issues and the clinical relevance of these findings are discussed.     

Porphyrins and Their Determination. A Review1

Using neutron activation analysis the mercury concentration in blood of non-exposed control persons has been shown to be 6–12 ng/g. In a group of persons exposed to methyl mercury compounds the mercury content in whole blood was 70–180 ng/g, in plasma 3–10 ng/g. A group of persons exposed to metal mercury vapors had a mercury concentration in whole blood of 12–90 ng/g, in plasma 5–74 ng/g. The mercury concentration in urine was hardly increased in the alkyl mercury group but there was a fair increase in the metal mercury group.

It is evident that the distribution of mercury in blood is quite different after exposure to different mercurials and so is the rate of excretion in urine. The distribution-excretion pattern may be used for diagnostic purposes.

The excretion of mercury cannot be used to evaluate the exposure to alkyl mercury compounds.     

A nine year follow-up study of patients with lymphoedema cholestasis syndrome 1 (LCS1/Aagenaes syndrome)

Abstract

The risks of developing energy or nutrient deficits are of great concern in infants and children with the rare lymphoedema cholestasis syndrome 1 (LCS1)/Aagenaes syndrome. In adolescents and adults, it is not known whether LCS1 patients need specific dietary advice outside periods of cholestasis. The primary objective of the present study was to evaluate the progression of the liver disease and nutritional status in patients with LCS1 over a period of nine years. Dietary and biochemical data were obtained for patients and healthy controls in two cross-sectional studies, a baseline (2000) and a follow-up study (2009). Thirteen patients above 18?years of age with LCS1 (65%) were included (six females). Dietary intake and biochemical measures were stable in the patients from baseline until follow-up. Compared to healthy controls, the patients had significantly higher serum levels of alkaline phosphatase (p?=?.015 and p?=?.002), gamma-glutamyltransferase (p?=?.001 and p?p?=?.037 and p?=?.016), and fibrinogen (p?=?.046 and p?p?=?.033 and p?p?=?.011 and p?=?.003) at baseline and follow-up. Despite stable liver function, the presence of a low grade of hepatobiliary dysfunction in these patients was suggested. Patients with LCS1 had a nutritional status similar to healthy controls, with no clinical deterioration of liver function during the nine-year period. The findings presented in this paper support that more than 50% of patients with LCS1 can expect a normal lifespan.     

脑多发性硬化1H-MR质子波谱分析应用研究

目的探讨1H-MRS技术在脑多发性硬化（multiple sclerosis,MS）的诊断及定性中的应用价值。材料与方法选择29例经临床确诊的MS患者进行MR及1H MRS检查,并与同期相同年龄组MRI显示正常者进行对照研究,计算1H-MRS代谢产物N-乙酰天冬氨酸（NAA）、肌酸（Cr）、胆碱复合物（Cho）及乳酸（LAC）的峰下面积,并对NAA/（Cr＋Cho）、Cho/Cr及LAC/Cr等各参数进行对比研究。结果MS组NAA/（Cr＋Cho）、Cho/Cr及LAC/Cr值分别为0.56、1.49及3.35,对照组分别为0.78、1.19及0.23。MS患者NAA/（Cr＋Cho）值下降,Cho/Cr、LAC/Cr值升高,差异有统计学意义（P值均〈0.05）。结论1H MRS有很高的特异性,可以为MS的早期诊断提供可靠的诊断信息,对指导临床治疗和判断预后有重要意义。     

甲型H1N1流感患者心理健康状况调查

目的：探讨甲型 H1N1流感患者的心理健康状况,为临床心理干预提供依据。方法对71例甲型H1N1流感患者采用症状自评量表进行测评分析。结果甲型 H1N1流感患者症状自评量表总均分、人际关系敏感、焦虑及恐怖因子分显著高于国内成人常模（ P＜0．05或0．01）,其他项目评分比较差异无显著性（ P＞0．05）。结论甲型 H1N1流感患者存在人际关系敏感、焦虑、恐怖等心理问题,在常规药物治疗的基础上联合有针对性的心理干预,对促进患者的早日康复具有重要意义。